Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 1,533,266 T→C Y262H (TAC→CAC)  PA14_RS07170 → enoyl‑CoA hydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084631,533,2660TC100.0% 1971.1 / NA 498Y262H (TAC→CAC) PA14_RS07170enoyl‑CoA hydratase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (232/266);  total (232/266)

Only 100 of 498 total aligned reads displayed.

GCGCAGATCAAGGAAGTGGTCCTGGCCGGCGCCGACCTGCCGCTGGACCAGGCCCTGGCGCTGGAGCGCAAGGCCTTCCAGTTGCTGTTCGACAGCCACGACCAGAAGGAGGGCATGCGTGCCTTCCTGGAAAAACGCACAGCGGAGTACCTGGGAAAATGAGCGAGCCATCGATCGTTCTCATCGGTATCGTCGGTACCGGTGCCATGGGCCAGGGCATCGCCCAGCTCGCTGCCTGTGCCGGGCTGTCGGTGCTGCTCTACGACAGCCGCCAGGGGGCGGCGCTGCAAGCGC  >  NC_008463/1533119‑1533412
                                                                                                                                                   |                                                                                                                                                  
gcgcAGATCAAGGAAGTGGTCCTGGCCGGCGCCGACCTGCCGCTGGACCAGGCCCTGGCGCTGGAGCGCAAGGCCTTCCAGTTGCTGTTCGACAGCCACGACCAGAAGGAGGGCATGCGTGCCTTCCTGGAAAAACGCACAGCGGAGCAc                                                                                                                                                  >  2:5338675/1‑150 (MQ=255)
  gcAGATCAAGGAAGTGGTCCTGGCCGGCGCCGACCTGCCGCTGGACCAGGCCCTGGCGCTGGAGCGCAAGGCCTTCCAGTTGCTGGTCGACAGCCACGACCAGAAGGAGGGCATGCGTGCCTTCCTGGAAAAACGCACAGCGGAGCACCt                                                                                                                                                >  2:261457/1‑150 (MQ=255)
   cAGATCAAGGAAGTGGTCCTGGCCGGCGCCGACCTGCCGCTGGACCAGGCCCTGGCGCTGGAGCGCAAGGCCTTCCAGTTGCTGTTCGACAGCCACGACCAGAAGGAGGGCATGCGTGCCTTCCTGGAAAAACGCACAGCGGAGCACCTg                                                                                                                                               <  2:3903348/150‑1 (MQ=255)
     gATCAAGGAAGTGGTCCTGGCCGGCGCCGACCTGCCGCTGGACCAGGCCCTGGCGCTGGAGCGCAAGGCCTTCCAGTTGCTGTTCGACAGCCACGACCAGAAGGAGGGCATGCGTGCCTTCCTGGAAAAACGCACAGCGGAGCACCTggg                                                                                                                                             >  2:9859712/1‑150 (MQ=255)
     gATCAAGGAAGTGGTCCTGGCCGGCGCCGACCTGCCGCTGGACCAGGCCCTGGCGCTGGAGCGCAAGGCCTTCCAGTTGCTGTTCGACAGCCACGACCAGAAGGAGGGCATGCGTGCCTTCCTGGAAAAACGCACAGCGGAGCACCTggg                                                                                                                                             >  1:6997436/1‑150 (MQ=255)
      aTCAAGGAAGTGGTCCTGGCCGGCGCCGACCTGCCGCTGGACCAGGCCCTGGCGCTGGAGCGCAAGGCCTTCCAGTTGCTGTTCGACAGCCACGACCAGAAGGAGGGCATGCGTGCCTTCCTGGAAAAACGCACAGCGGAGCACCTGGGa                                                                                                                                            <  2:9566530/150‑1 (MQ=255)
      aTCAAGGAAGTGGTCCTGGCCGGCGCCGACCTGCCGCTGGACCAGGCCCTGGCGCTGGAGCGCAAGGCCTTCCAGTTGCTGTTCGACAGCCACGACCAGAAGGAGGGCATGCGTGCCTTCCTGGAAAAACGCACAGCGGAGCACCTGGGa                                                                                                                                            <  2:8929691/150‑1 (MQ=255)
       tCAAGGAAGTGGTCCTGGCCGGCGCCGACCTGCCGCTGGACCAGGCCCTGGCGCTGGAGCGCAAGGCCTTCCAGTTGCTGTTCGACAGCCACGACCAGAAGGAGGGCATGCGTGCCTTCCTGGAAAAACGCACAGCGGAGCACCTGGGaa                                                                                                                                           >  1:1211109/1‑150 (MQ=255)
       tCAAGGAAGTGGTCCTGGCCGGCGCCGACCTGCCGCTGGACCAGGCCCTGGCGCTGGAGCGCAAGGCCTTCCAGTTGCTGTTCGACAGCCACGACCAGAAGGAGGGCATGCGTGCCTTCCTGGAAAAACGCACAGCGGAGCACCTGGGaa                                                                                                                                           <  1:6058515/150‑1 (MQ=255)
        cAAGGAAGTGGTCCTGGCCGGCGCCGACCTGCCGCTGGACCAGGCCCTGGCGCTGGAGCGCAAGGCCTTCCAGTTGCTGTTCGACAGCCACGACCAGAAGGAGGGCATGCGTGCCTTCCTGGAAAAACGCACAGCGGAGCACCTGGGaaa                                                                                                                                          >  2:814476/1‑150 (MQ=255)
         aaGGAAGTGGTCCTGGCCGGCGCCGACCTGCCGCTGGACCAGGCCCTGGCGCTGGAGCGCAAGGCCTTCCAGTTGCTGTTCGACAGCCACGACCAGAAGGAGGGCATGCGTGCCTTCCTGGAAAAACGCACAGCGGAGCACCTGGGaaaa                                                                                                                                         <  2:1523057/150‑1 (MQ=255)
            gAAGTGGTCCTGGCCGGCGCCGACCTGCCGCTGGACCAGGCCCTGGCGCTGGAGCGCAAGGCCTTCCAGTTGCTGTTCGACAGCCACGACCAGAAGGAGGGCATGCGTGCCTTCCTGGAAAAACGCACAGCGGAGCACCTGGGAAAATga                                                                                                                                      <  1:5511081/150‑1 (MQ=255)
              aGTGGTCCTGGCCGGCGCCGACCTGCCGCTGGACCAGGCCCTGGCGCTGGAGCGCAAGGCCTTCCAGTTGCTGTTCGACAGCCACGACCAGAAGGAGGGCATGCGTGCCTTCCTGGAAAAACGCACAGCGGAGCACCTGGGAAAATgagc                                                                                                                                    >  2:2800864/1‑150 (MQ=255)
                tGGTCCTGGCCGGCGCCGACCTGCCGCTGGACCAGGCCCTGGCGCTGGAGCGCAAGGCCTTCCAGTTGCGGTTCGACAGCCACGACCAGAAGGAGGGCATGCGTGCCTTCCTGGAAAAACGCACAGCGGAGCACCTGGGAAAATgagcga                                                                                                                                  <  2:6124531/150‑1 (MQ=255)
                 ggTCCTGGCCGGCGCCGACCTGCCGCTGGACCAGGCCCTGGCGCTGGAGCGCAAGGCCTTCCAGTTGCTGTTCGACAGCCACGACCAGAAGGAGGGCATGCGTGCCTTCCTGGAAAAACGCACAGCGGAGCACCTGGGAAAATgagcgag                                                                                                                                 <  1:4117932/150‑1 (MQ=255)
                  gTCCTGGCCGGCGCCGACCTGCCGCTGGACCAGGCCCTGGCGCTGGAGCGCAAGGCCTTCCAGTTGCTGTTCGACAGCCACGACCAGAAGGAGGGCATGCGTGCCTTCCGGGAAAAACGCACAGCGGAGCACCTGGGAAAATgagcgagc                                                                                                                                >  1:7624029/1‑150 (MQ=255)
                     cTGGCCGGCGCCGACCTGCCGCTGGACCAGGCCCTGGCGCTGGAGCGCAAGGCCTTCCAGTTGCTGTTCGACAGCCACGACCAGAAGGAGGGCATGCGTGCCTTCCTGGAAAAACGCACAGCGGAGCACCTGGGAAAATGAGCGAGCCat                                                                                                                             <  1:9791347/150‑1 (MQ=255)
                      tGGCCGGCGCCGACCTGCCGCTGGACCAGGCCCTGGCGCTGGAGCGCAAGGCCTTCCAGTTGCTGTTCGACAGCCACGACCAGAAGGAGGGCATGCGTGCCTTCCTGGAAAAACGCACAGCGGAGCACCTGGGAAAATGAGCGAGCCatc                                                                                                                            <  1:7221195/150‑1 (MQ=255)
                       ggCCGGCGCCGACCTGCCGCTGGACCAGGCCCTGGCGCTGGAGCGCAAGGCCTTCCAGTTGCTGTTCGACAGCCACGACCAGAAGGAGGGCATGCGTGCCTTCCTGGAAAAACGCACAGCGGAGCACCTGGGAAAATGAGCGAGCCatcg                                                                                                                           >  1:2792805/1‑150 (MQ=255)
                        gCCGGCGCCGACCTGCCGCTGGACCAGGCCCTGGCGCTGGAGCGCAAGGCCTTCCAGTTGCTGTTCGACAGCCACGACCAGAAGGAGGGCATGCGTGCCTTCCTGGAAAAACGCACAGCGGAGCACCTGGGAAAATGAGCGAGCCatcga                                                                                                                          >  1:877435/1‑150 (MQ=255)
                         ccGGCGCCGACCTGCCGCTGGACCAGGCCCTGGCGCTGGAGCGCAAGGCCTTCCAGTTGCTGTTCGACAGCCACGACCAGAAGGAGGGCATGCGTGCCTTCCTGGAAAAACGCACAGCGGAGCACCTGGGAAAATGAGCGAGCCatcgat                                                                                                                         >  2:10222875/1‑150 (MQ=255)
                            gcgcCGACCTGCCGCTGGACCAGGCCCTGGCGCTGGAGCGCAAGGCCTTCCAGTTGCTGTTCGACAGCCACGACCAGAAGGAGGGCATGCGTGCCTTCCTGGAAAAACGCACAGCGGAGCACCTGGGAAAATGAGCGAGCCATCGATCGt                                                                                                                      >  1:9441788/1‑150 (MQ=255)
                               ccGACCTGCCGCTGGACCAGGCCCTGGCGCTGGAGCGCAAGGCCTTCCAGTTGCTGTTCGACAGCCACGACCAGAAGGAGGGCATGCGTGCCTTCCTGGAAAAACGCACAGCGGAGCACCTGGGAAAATGAGCGAGCCATCGATCGTtct                                                                                                                   >  1:572321/1‑150 (MQ=255)
                               ccGACCTGCCGCTGGACCAGGCCCTGGCGCTGGAGCGCAAGGCCTTCCAGTTGCTGTTCGACAGCCACGACCAGAAGGAGGGCATGCGTGCCTTCCTGGAAAAACGCACAGCGGAGCACCTGGGAAAATGAGCGAGCCATCGATCGTtct                                                                                                                   <  2:681043/150‑1 (MQ=255)
                               ccGACCTGCCGCTGGACCAGGCCCTGGCGCTGGAGCGCAAGGCCTTCCAGTTGCTGTTCGACAGCCACGACCAGAAGGAGGGCATGCGTGCCTTCCTGGAAAAACGCACAGCGGAGCACCTGGGAAAATGAGCGAGCCATCGATCGTtct                                                                                                                   <  1:2733309/150‑1 (MQ=255)
                                cGACCTGCCGCTGGACCAGGCCCTGGCGCTGGAGCGCAAGGCCTTCCAGTTGCTGTTCGACAGCCACGACCAGAAGGAGGGCATGCGTGCCTTCCTGGAAAAACGCACAGCGGAGCACCTGGGAAAATGAGCGAGCCATCGATCGTtctc                                                                                                                  <  1:7767288/150‑1 (MQ=255)
                                     tGCCGCTGGACCAGGCCCTGGCGCTGGAGCGCAAGGCCTTCCAGTTGCTGTTCGACAGCCACGACCAGAAGGAGGGCATGCGTGCCTTCCTGGAAAAACGCACAGCGGAGCACCTGGGAAAATGAGCGAGCCATCGATCGTTCTCATCgg                                                                                                             >  1:8683393/1‑150 (MQ=255)
                                     tGCCGCTGGACCAGGCCCTGGCGCTGGAGCGCAAGGCCTTCCAGTTGCTGTTCGACAGCCACGACCAGAAGGAGGGCATGCGTGCCTTCCTGGAAAAACGCACAGCGGAGCACCTGGGAAAATGAGCGAGCCATCGATCGTTCTCATCgg                                                                                                             >  1:6499941/1‑150 (MQ=255)
                                      gCCGCTGGACCAGGCCCTGGCGCTGGAGCGCAAGGCCTTCCAGTTGCTGTTCGACAGCCACGACCAGAAGGAGGGCATGCGTGCCTTCCTGGAAAAACGCACAGCGGAGCACCTGGGAAAATGAGCGAGCCATCGATCGTTCTCATCGGt                                                                                                            <  2:4757955/150‑1 (MQ=255)
                                        cGCTGGACCAGGCCCTGGCGCTGGAGCGCAAGGCCTTCCAGTTGCTGTTCGACAGCCACGACCAGAAGGAGGGCATGCGTGCCTTCCTGGAAAAACGCACAGCGGAGCACCTGGGAAAATGAGCGAGCCATCGATCGTTCTCATCGGTAt                                                                                                          >  2:3269568/1‑150 (MQ=255)
                                        cGCTGGACCAGGCCCTGGCGCTGGAGCGCAAGGCCTTCCAGTTGCTGTTCGACAGCCACGACCAGAAGGAGGGCATGCGTGCCTTCCTGGAAAAACGCACAGCGGAGCACCTGGGAAAATGAGCGAGCCATCGATCGTTCTCATCGGTAt                                                                                                          <  1:2189341/150‑1 (MQ=255)
                                        cGCTGGACCAGGCCCTGGCGCTGGAGCGCAAGGCCTTCCAGTTGCTGTTCGACAGCCACGACCAGAAGGAGGGCATGCGTGCCTTCCTGGAAAAACGCACAGCGGAGCACCTGGGAAAATGAGCGAGCCATCGATCGTTCTCATCGGTAt                                                                                                          >  2:8616202/1‑150 (MQ=255)
                                         gCTGGACCAGGCCCTGGCGCTGGAGCGCAAGGCCTTCCAGTTGCTGTTCGACAGCCACGACCAGAAGGAGGGCATGCGTGCCTTCCTGGAAAAACGCACAGCGGAGCACCTGGGAAAATGAGCGAGCCATCGATCGTTCTCATCGGTAtc                                                                                                         <  2:2352531/150‑1 (MQ=255)
                                         gCTGGACCAGGCCCTGGCGCTGGAGCGCAAGGCCTTCCAGTTGCTGTTCGACAGCCACGACCAGAAGGAGGGCATGCGTGCCTTCCTGGAAAAACGCACAGCGGAGCACCTGGGAAAATGAGCGAGCCATCGATCGTTCTCATCGGTAtc                                                                                                         >  1:8425082/1‑150 (MQ=255)
                                         gCTGGACCAGGCCCTGGCGCTGGAGCGCAAGGCCTTCCAGTTGCTGTTCGACAGCCACGACCAGAAGGAGGGCATGCGTGCCTTCCTGGAAAAACGCACAGCGGAGCACCTGGGAAAATGAGCGAGCCATCGATCGTTCTCATCGGTAtc                                                                                                         <  2:4675379/150‑1 (MQ=255)
                                              aCCAGGCCCTGGCGCTGGAGCGCAAGGCCTTCCAGTTGCTGTTCGACAGCCACGACCAGAAGGAGGGCATGCGTGCCTTCCTGGAAAAACGCACAGCGGAGCACCTGGGAAAATGAGCGAGCCATCGATCGTTCTCATCGGTATCGTCgg                                                                                                    <  1:1414850/150‑1 (MQ=255)
                                                cAGGCCCTGGCGCTGGAGCGCAAGGCCTTCCAGTTGCTGTTCGACAGCCACGACCAGAAGGAGGGCATGCGTGCCTTCCTGGAAAAACGCACAGCGGAGCACCTGGGAAAATGAGCGAGCCATCGATCGTTCTCATCGGTATCGTCGGTa                                                                                                  >  1:7121211/1‑150 (MQ=255)
                                                 aGGCCCTGGCGCTGGAGCGCAAGGCCTTCCAGTTGCTGTTCGACAGCCACGACCAGAAGGAGGGCATGCGTGCCTTCCTGGAAAAACGCACAGCGGAGCACCTGGGAAAATGAGCGAGCCATCGATCGTTCTCATCGGTATCGTCGGTAc                                                                                                 <  1:6195281/150‑1 (MQ=255)
                                                   gCCCTGGCGCTGGAGCGCAAGGCCTTCCAGTTGCTGTTCGACAGCCACGACCAGAAGGAGGGCATGCGTGCCTTCCTGGAAAAACGCACAGCGGAGCACCTGGGAAAATGAGCGAGCCATCGATCGTTCTCATCGGTATCGTCGGTACCg                                                                                               <  2:3592560/150‑1 (MQ=255)
                                                    cccTGGCGCTGGAGCGCAAGGCCTTCCAGTTGCTGTTCGACAGCCACGACCAGAAGGAGGGCATGCGTGCCTTCCTGGAAAAACGCACAGCGGAGCACCTGGGAAAATGAGCGAGCCATCGATCGTTCTCATCGGTATCGTCGGTACCgg                                                                                              >  2:7498775/1‑150 (MQ=255)
                                                        ggCGCTGGAGCGCAAGGCCTTCCAGTTGCTGGTCGACAGCCACGACCAGAAGGAGGGCATGCGTGCCTTCCTGGAAAAACGCACAGCGGAGCACCTGGGAAAATGAGCGAGCCATCGATCGTTCTCATCGGTATCGTCGGTACCGGTGcc                                                                                          <  2:10063879/150‑1 (MQ=255)
                                                           gcTGGAGCGCAAGGCCTTCCAGTTGCTGTTCGACAGCCACGACCAGAAGGAGGGCATGCGTGCCTTCCTGGAAAAACGCACAGCGGAGCACCTGGGAAAATGAGCGAGCCATCGATCGTTCTCATCGGTATCGTCGGTACCGGTGCCATg                                                                                       >  2:1281668/1‑150 (MQ=255)
                                                            cTGGAGCGCAAGGCCTTCCAGTTGCTGTTCGACAGCCACGACCAGAAGGAGGGCATGCGTGCCTTCCTGGAAAAACGCACAGCGGAGCACCTGGGAAAATGAGCGAGCCATCGATCGTTCTCATCGGTATCGTCGGTACCGGTGCCATgg                                                                                      <  1:10060594/150‑1 (MQ=255)
                                                                aGCGCAAGGCCTTCCAGTTGCTGTTCGACAGCCACGACCAGAAGGAGGGCATGCGTGCCTTCCTGGAAAAACGCACAGCGGAGCACCTGGGAAAATGAGCGAGCCATCGATCGTTCTCATCGGTATCGTCGGTACCGGTGCCATGGGCCa                                                                                  <  2:8423124/150‑1 (MQ=255)
                                                                aGCGCAAGGCCTTCCAGTTGCTGTTCGACAGCCACGACCAGAAGGAGGGCATGCGTGCCTTCCTGGAAAAACGCACAGCGGAGCACCTGGGAAAATGAGCGAGCCATCGATCGTTCTCATCGGTATCGTCGGTACCGGTGCCATGGGCCa                                                                                  <  1:10409910/150‑1 (MQ=255)
                                                                 gcgcAAGGCCTTCCAGTTGCTGTTCGACAGCCACGACCAGAAGGAGGGCATGCGTGCCTTCCTGGAAAAACGCACAGCGGAGCACCTGGGAAAATGAGCGAGCCATCGATCGTTCTCATCGGTATCGTCGGTACCGGTGCCCTGGGCCAg                                                                                 >  1:9257995/1‑150 (MQ=255)
                                                                 gcgcAAGGCCTTCCAGTTGCTGTTCGACAGCCACGACCAGAAGGAGGGCATGCGTGCCTTCCTGGAAAAACGCACAGCGGAGCACCTGGGAAAATGAGCGAGCCATCGATCGTTCTCATCGGTATCGTCGGTACCGGTGCCATGGGCCAg                                                                                 <  1:497416/150‑1 (MQ=255)
                                                                 gcgcAAGGCCTTCCAGTTGCTGTTCGACAGCCACGACCAGAAGGAGGGCATGCGTGCCTTCCTGGAAAAACGCACAGCGGAGCACCTGGGAAAATGAGCGAGCCATCGATCGTTCTCATCGGTATCGTCGGTACCGGTGCCATGGGCCAg                                                                                 <  1:2063904/150‑1 (MQ=255)
                                                                  cgcAAGGCCTTCCAGTTGCTGTTCGACAGCCACGACCAGAAGGAGGGCATGCGTGCCTTCCTGGAAAAACGCACAGCGGAGCACCTGGGAAAATGAGCGAGCCATCGATCGTTCTCATCGGTATCGTCGGTACCGGTGCCATGGGCCAgg                                                                                <  2:6536158/150‑1 (MQ=255)
                                                                     aaGGCCTTCCAGTTGCTGTTCGACAGCCACGACCAGAAGGAGGGCATGCGTGCCTTCCTGGAAAAACGCACAGCGGAGCACCTGGGAAAATGAGCGAGCCATCGATCGTTCTCATCGGTATCGTCGGTACCGGTGCCATGGGCCAGGGCa                                                                             <  1:3480122/150‑1 (MQ=255)
                                                                            tCCAGTTGCTGTTCGACAGCCACGACCAGAAGGAGGGCATGCGTGCCTTCCTGGAAAAACGCACAGCGGAGCACCTGGGAAAATGAGCGAGCCATCGATCGTTCTCATCGGTATCGTCGGTACCGGTGCCATGGGCCAGGGCATCGCCCa                                                                      >  1:5830437/1‑150 (MQ=255)
                                                                                gTTGCTGTTCGACAGCCACGACCAGAAGGAGGGCATGCGTGCCTTCCTGGAAAAACGCACAGCGGAGCACCTGGGAAAATGAGCGAGCCCTCGATCGTTCTCATCGGTATCGTCGGTACCGGTGCCATGGGCCAGGGCATCGCCCAGCTc                                                                  >  1:3801712/1‑150 (MQ=255)
                                                                                gTTGCTGTTCGACAGCCACGACCAGAAGGAGGGCATGCGTGCCTTCCTGGAAAAACGCACAGCGGAGCACCTGGGAAAATGAGCGAGCCATCGATCGTTCTCATCGGTATCGTCGGTACCGGTGCCATGGGCCAGGGCATCGCCCAGCTc                                                                  <  1:1589049/150‑1 (MQ=255)
                                                                                 ttGCTGTTCGACAGCCACGACCAGAAGGAGGGCATGCGTGCCTTCCTGGAAAAACGCACAGCGGAGCACCTGGGAAAATGAGCGAGCCATCGATCGTTCTCATCGGTATCGTCGGTACCGGTGCCATGGGCCAGGGCATCGCCCAGCTCg                                                                 >  1:2913903/1‑150 (MQ=255)
                                                                                  tGCTGTTCGACAGCCACGACCAGAAGGAGGGCATGCGTGCCTTCCTGGAAAAACGCACAGCGGAGCACCTGGGAAAATGAGCGAGCCATCGATCGTTCTCATCGGTATCGTCGGTACCGGTGCCATGGGCCAGGGCATCGCCCAGCTCGc                                                                <  2:9194466/150‑1 (MQ=255)
                                                                                  tGCTGTTCGACAGCCACGACCAGAAGGAGGGCATGCGTGCCGTCCTGGAAAAACGCACAGCGGAGCACCTGGGAAAATGAGCGAGCCATCGATCGTTCTCATCGGTATCGTCGGTACCGGTGCCATGGGCCAGGGCATCGCCCAGCTCGc                                                                <  1:2431844/150‑1 (MQ=255)
                                                                                       ttCGACAGCCACGACCAGAAGGAGGGCATGCGTGCCTTCCTGGAAAAACGCACAGCGGAGCACCTGGGAAAATGAGCGAGCCATCGATCGTTCTCATCGGTATCGTCGGTACCGGTGCCATGGGCCAGGGCATCGCCCAGCTCGCTGCCt                                                           <  1:3069526/150‑1 (MQ=255)
                                                                                       ttCGACAGCCACGACCAGAAGGAGGGCATGCGTGCCTTCCTGGAAAAACGCACAGCGGAGCACCTGGGAAAATGAGCGAGCCATCGATCGTTCTCATCGGTATCGTCGGTACCGGTGCCATGGGCCAGGGCATCGCCCAGCTCGCTGCCt                                                           <  2:9385569/150‑1 (MQ=255)
                                                                                        tCGACAGCCACGACCAGAAGGAGGGCATGCGTGCCTTCCTGGAAAAACGCACAGCGGAGCACCTGGGAAAATGAGCGAGCCATCGATCGTTCTCATCGGTATCGTCGGTACCGGTGCCATGGGCCAGGGCATCGCCCAGCTCGCTGCCtg                                                          <  2:10818448/150‑1 (MQ=255)
                                                                                             aGCCACGACCAGAAGGAGGGCATGCGTGCCTTCCTGGAAAAACGCACAGCGGAGCACCTGGGAAAATGAGCGAGCCATCGATCGTTCTCATCGGTATCGTCGGTACCGGTGCCATGGGCCAGGGCATCGCCCAGCTCGCTGCCTGTGCCg                                                     <  1:7943970/150‑1 (MQ=255)
                                                                                             aGCCACGACCAGAAGGAGGGCATGCGTGCCTTCCTGGAAAAACGCACAGCGGAGCACCTGGGAAAATGAGCGAGCCATCGATCGTTCTCATCGGTATCGTCGGTACCGGTGCCATGGGCCAGGGCATCGCCCAGCTCGCTGCCTGTGCCg                                                     <  2:8774783/150‑1 (MQ=255)
                                                                                              gCCACGACCAGAAGGAGGGCATGCGTGCCTTCCTGGAAAAACGCACAGCGGAGCACCTGGGAAAATGAGCGAGCCATCGATCGTTCTCATCGGTATCGTCGGTACCGGTGCCATGGGCCAGGGCATCGCCCAGCTCGCTGCCTGTGCCgg                                                    <  2:5773737/150‑1 (MQ=255)
                                                                                                 aCGACCAGAAGGAGGGCATGCGTGCCTTCCTGGAAAAACGCACAGCGGAGCACCTGGGAAAATGAGCGAGCCATCGATCGTTCTCATCGGTATCGTCGGTACCGGTGCCATGGGCCAGGGCATCGCCCAGCTCGCTGCCTGTGCCGGGCt                                                 >  1:8917149/1‑150 (MQ=255)
                                                                                                 aCGACCAGAAGGAGGGCATGCGTGCCTTCCTGGAAAAACGCACAGCGGAGCACCTGGGAAAATGAGCGAGCCATCGATCGTTCTCATCGGTATCGTCGGTACCGGTGCCATGGGCCAGGGCATCGCCCAGCTCGCTGCCTGTGCCGGGCt                                                 >  1:5119059/1‑150 (MQ=255)
                                                                                                   gACCAGAAGGAGGGCATGCGTGCCTTCCGGGAAAAACGCACAGCGGAGCACCCGGGAAAATGTGCGAGCCATCGTTCGTTCTCATCGGTATCGTCGGTACCGGTGCCTTGTGCCTGGGCATCGCCCAGCTCGCTGCCTGTGCCGGGCTGt                                               <  1:3218389/150‑1 (MQ=255)
                                                                                                     ccAGAAGGAGGGCATGCGTGCCTTCCTGGAAAAACGCACAGCGGAGCACCTGGGAAAATGAGCGAGCCATCGATCGTTCTCATCGGTATCGTCGGTACCGGTGCCATGGGCCAGGGCATCGCCCAGCTCGCTGCCTGTGCCGGGCTGTCg                                             >  1:4848784/1‑150 (MQ=255)
                                                                                                     ccAGAAGGAGGGCATGCGTGCCTTCCTGGAAAAACGCACAGCGGAGCACCTGGGAAAATGAGCGAGCCATCGATCGTTCTCATCGGTATCGTCGGTACCGGTGCCATGGGCCAGGGCATCGCCCAGCTCGCTGCCTGTGCCGGGCTGTCg                                             >  1:7355432/1‑150 (MQ=255)
                                                                                                      cAGAAGGAGGGCATGCGTGCCTTCCTGGAAAAACGCACAGCGGAGCACCTGGGAAAATGAGCGAGCCATCGATCGTTCTCATCGGTATCGTCGGTACCGGTGCCATGGGCCAGGGCATCGCCCAGCTCGCTGCCTGTGCCGGGCTGTCgg                                            <  1:6643224/150‑1 (MQ=255)
                                                                                                      cAGAAGGAGGGCATGCGTGCCTTCCTGGAAAAACGCACAGCGGAGCACCTGGGAAAATGAGCGAGCCATCGATCGTTCTCATCGGTATCGTCGGTACCGGTGCCATGGGCCAGGGCATCGCCCAGCTCGCTGCCTGTGCCGGGCTGTCgg                                            >  2:7928884/1‑150 (MQ=255)
                                                                                                      cAGAAGGAGGGCATGCGTGCCTTCCTGGAAAAACGCACAGCGGAGCACCTGGGAAAATGAGCGAGCCATCGATCGTTCTCATCGGTATCGTCGGTACCGGTGCCATGGGCCAGGGCATCGCCCAGCTCGCTGCCTGTGCCGGGCTGTCgg                                            <  2:6956254/150‑1 (MQ=255)
                                                                                                        gAAGGAGGGCATGCGTGCCTTCCTGGAAAAACGCACAGCGGAGCACCTGGGAAAATGAGCGAGCCATCGATCGTTCTCATCGGTATCGTCGGTACCGGTGCCATGGGCCAGGGCATCGCCCAGCTCGCTGCCTGTGCCGGGCTGTCGGtg                                          >  2:10627734/1‑150 (MQ=255)
                                                                                                        gAAGGAGGGCATGCGTGCCTTCCTGGAAAAACGCACAGCGGAGCACCTGGGAAAATGAGCGAGCCATCGATCGTTCTCATCGGTATCGTCGGTACCGGTGCCATGGGCCAGGGCATCGCCCAGCTCGCTGCCTGTGCCGGGCTGTCGGtg                                          >  1:9886340/1‑150 (MQ=255)
                                                                                                         caggaggGCATGCGTGCCTTCCTGGAAAAACGCACAGCGGAGCACCTGGGAAAATGAGCGAGCCATCGATCGTTCTCATCGGTATCGTCGGTACCGGTGCCATGGGCCAGGGCATCGCCCAGCTCGCTGCCTGTGCCGGGCTGTCGGtgc                                         <  2:7344542/149‑1 (MQ=255)
                                                                                                          aggaggGCCTGCGTGCCTTCCTGGAAAAACGCACAGCGGAGCACCTGGGAAAATGAGCGAGCCATCGATCGTTCTCATCGGTATCGTCGGTACCGGTGCCATGGGCCAGGGCATCGCCCAGCTCGCTGCCTGTGCCGGGCTGTCGGtgct                                        <  1:10607318/150‑1 (MQ=255)
                                                                                                          aggaggGCATGCGTGCCTTCCTGGAAAAACGCACAGCGGAGCACCTGGGAAAATGAGCGAGCCATCGATCGTTCTCATCGGTATCGTCGGTACCGGTGCCATGGGCCAGGGCATCGCCCAGCTCGCTGCCTGTGCCGGGCTGTCGGtgct                                        >  1:4323835/1‑150 (MQ=255)
                                                                                                              gggCATGCGTGCCTTCCTGGAAAAACGCACAGCGGAGCACCTGGGAAAATGAGCGAGCCATCGATCGTTCTCATCGGTATCGTCGGAACCGGTGCCATGGGCCAGGGCCTCGCCCAGCTCGCTGCCTGTGCCGGGCTGTCGGTGCTGctc                                    >  1:7462036/1‑150 (MQ=255)
                                                                                                                gCATGCGTGCCTTCCTGGAAAAACGCACAGCGGAGCACCTGGGAAAATGAGCGAGCCATCGATCGTTCTCATCGGTATCGTCGGTACCGGTGCCATGGGCCAGGGCATCGCCCAGCTCGCTGCCTGTGCCGGGCTGTCGGTGCTGCTCTa                                  <  2:3785733/150‑1 (MQ=255)
                                                                                                                gCATGCGTGCCTTCCTGGAAAAACGCACAGCGGAGCACCTGGGAAAATGAGCGAGCCATCGATCGTTCTCATCGGTATCGTCGGTACCGGTGCCATGGGCCAGGGCATCGCCCAGCTCGCTGCCTGTGCCGGGCTGTCGGTGCTGCTCTa                                  >  1:4503538/1‑150 (MQ=255)
                                                                                                                   tGCGTGCCTTCCTGGAAAAACGCACAGCGGAGCACCTGGGAAAATGAGCGAGCCATCGATCGTTCTCATCGGTATCGTCGGTACCGGTGCCATGGGCCAGGGCATCGCCCAGCTCGCTGCCTGTGCCGGGCTGTCGGTGCTGCTCTACGa                               >  1:4630735/1‑150 (MQ=255)
                                                                                                                    gCGTGCCTTCCTGGAAAAACGCACAGCGGAGCACCTGGGAAAATGAGCGAGCCATCGATCGTTCTCATCGGTATCGTCGGTACCGGTGCCATGGGCCAGGGCATCGCCCAGCTCGCTGCCTGTGCCGGGCTGTCGGTGCTGCTCTACGAc                              <  2:4968464/150‑1 (MQ=255)
                                                                                                                    gCGTGCCTTCCTGGAAAAACGCACAGCGGAGCACCTGGGAAAATGAGCGAGCCATCGATCGTTCTCATCGGTATCGTCGGTACCGGTGCCATGGGCCAGGGCATCGCCCAGCTCGCTGCCTGTGCCGGGCTGTCGGTGCTGCTCTACGAc                              <  1:2034355/150‑1 (MQ=255)
                                                                                                                    gCGTGCCTTCCTGGAAAAACGCACAGCGGAGCACCTGGGAAAATGAGCGAGCCATCGATCGTTCTCATCGGTATCGTCGGTACCGGTGCCATGGGCCAGGGCATCGCCCAGCTCGCTGCCTGTGCCGGGCTGTCGGTGCTGCTCTACGAc                              <  1:8841008/150‑1 (MQ=255)
                                                                                                                         ccTTCCTGGAAAAACGCACAGCGGAGCACCTGGGAAAATGAGCGAGCCATCGATCGTTCTCATCGGTATCGTCGGTACCGGTGCCATGGGCCAGGGCATCGCCCAGCTCGCTGCCTGTGCCGGGCTGTCGGTGCTGCTCTACGACAgccg                         >  1:9138638/1‑150 (MQ=255)
                                                                                                                          cTTCCTGGAAAAACGCACAGCGGAGCACCTGGGAAAATGAGCGAGCCATCGATCGTTCTCATCGGTATCGTCGGTACCGGTGCCATGGGCCAGGGCATCGCCCAGCTCGCTGCCTGTGCCGGGCTGTCGGTGCTGCTCTACGACAgccgc                        <  2:10392843/150‑1 (MQ=255)
                                                                                                                            tCCTGGAAAAACGCACAGCGGAGCACCTGGGAAAATGAGCGAGCCATCGATCGTTCTCATCGGTATCGTCGGTACCGGTGCCATGGGCCAGGGCATCGCCCAGCTCGCTGCCTGTGCCGGGCTGTCGGTGCTGCTCTACGACAGCCGCCa                      >  2:4045103/1‑150 (MQ=255)
                                                                                                                              cTGGAAAAACGCACAGCGGAGCACCTGGGAAAATGAGCGAGCCATCGATCGTTCTCATCGGTATCGTCGGTACCGGTGCCATGGGCCCGGGCATCGCCCAGCTCGCTGCCGGTGCCGGGCTGTCGGTGGTGCTCTACGACAGCCGCCAgg                    >  2:4192541/1‑150 (MQ=255)
                                                                                                                                 gAAAAACGCACAGCGGAGCACCTGGGAAAATGAGCGAGCCATCGATCGTTCTCATCGGTATCGTCGGTACCGGTGCCATGGGCCAGGGCATCGCCCAGCTCGCTGCCTGTGCCGGGCTGTCGGTGCTGCTCTACGACAGCCGCCAggggg                 <  2:446861/150‑1 (MQ=255)
                                                                                                                                  aaaaaCGCACAGCGGAGCACCTGGGAAAATGAGCGAGCCATCGATCGTTCTCATCGGTATCGTCGGTACCGGTGCCATGGGCCAGGGCATCGCCCAGCTCGCTGCCTGTGCCGGGCTGTCGGTGCTGCTCTACGACAGCCGCCAGGGggc                >  2:2182820/1‑150 (MQ=255)
                                                                                                                                    aaaCGCACTGCGGAGCACCTGGGAAAATGAGCGAGCCATCGATCGTTCTCATCGGTATCGTCGGTACCGGTGCCATGGGCCAGGGCATCGCCCAGCTCGCTGCCTGTGCCGGGCTGTCGGTGCTGCTCTACGACAGCCGCCAGGGggcgg              <  2:670314/150‑1 (MQ=255)
                                                                                                                                    aaaCGCACAGCGGAGCACCTGGGAAAATGAGCGAGCCATCGATCGTTCTCATCGGTATCGTCGGTACCGGTGCCATGGGCCAGGGCATCGCCCAGCTCGCTGCCTGTGCCGGGCTGTCGGTGCTGCTCTACGACAGCCGCCAGGGggcgg              <  2:5494075/150‑1 (MQ=255)
                                                                                                                                        gCACAGCGGAGCACCTGGGAAAATGAGCGAGCCATCGATCGTTCTCATCGGTATCGTCGGTACCGGTGCCATGGGCCAGGGCATCGCCCAGCTCGCTGCCTGTGCCGGGCTGTCGGTGCTGCTCTACGACAGCCGCCAGGGGGCGGCGCt          >  1:10279959/1‑150 (MQ=255)
                                                                                                                                         cacaGCGGAGCACCTGGGAAAATGAGCGAGCCATCGATCGTTCTCATCGGTATCGTCGGTACCGGTGCCATGGGCCAGGGCATCGCCCAGCTCGCTGCCTGTGCCGGGCTGTCGGTGCTGCTCTACGACAGCCGCCAGGGGGCGGCGCTg         <  1:11110925/150‑1 (MQ=255)
                                                                                                                                          acaGCGGAGCACCTGGGAAAATGAGCGAGCCATCGATCGTTCTCATCGGTATCGTCGGTACCGGTGCCATGGGCCAGGGCATCGCCCAGCTCGCTGCCTGTGCCGGGCTGTCGGTGCTGCTCTACGACAGCCGCCAGGGGGCGGCGCTGc        <  1:1307581/150‑1 (MQ=255)
                                                                                                                                          acaGCGGAGCACCTGGGAAAATGAGCGAGCCATCGATCGTTCTCATCGGTATCGTCGGTACCGGTGCCATGGGCCAGGGCATCGCCCAGCTCGCTGCCTGTGCCGGGCTGTCGGTGCTGCTCTACGACAGCCGCCAGGGGGCGGCGCTGc        >  1:10770722/1‑150 (MQ=255)
                                                                                                                                           caGCGGAGCACCTGGGAAAATGAGCGAGCCATCGATCGTTCTCATCGGTATCGTCGGTACCGGTGCCATGGGCCAGGGCATCGCCCAGCTCGCTGCCTGTGCCGGGCTGTCGGTGCTGCTCTACGACAGCCGCCAGGGGGCGGCGCTGCa       <  1:5271992/150‑1 (MQ=255)
                                                                                                                                             gCGGAGCACCTGGGAAAATGAGCGAGCCATCGATCGTTCTCATCGGTATCGTCGGTACCGGTGCCATGGGCCAGGGCATCGCCCAGCTCGCTGCCTGTGCCGGGCTGTCGGTGCTGCTCTACGACAGCCGCCAGGGGGCGGCGCTGCAAg     >  1:1716803/1‑150 (MQ=255)
                                                                                                                                             gCGGAGCACCTGGGAAAATGAGCGAGCCATCGATCGTTCTCATCGGTATCGTCGGTACCGGTGCCATGGGCCAGGGCATCGCCCAGCTCGCTGCCTGTGCCGGGCTGTCGGTGCTGCTCTACGACAGCCGCCAGGGGGCGGCGCTGCAAg     <  2:9299421/150‑1 (MQ=255)
                                                                                                                                             gCGGAGCACCTGGGAAAATGAGCGAGCCATCGATCGTTCTCATCGGTATCGTCGGTACCGGTGCCATGGGCCAGGGCATCGCCCAGCTCGCTGCCTGTGCCGGGCTGTCGGTGCTGCTCTACGACAGCCGCCAGGGGGCGGCGCTGCAAg     <  2:11035419/150‑1 (MQ=255)
                                                                                                                                               ggAGCACCTGGGAAAATGAGCGAGCCATCGATCGTTCTCATCGGTATCGTCGGTACCGGTGCCATGGGCCAGGGCATCGCCCAGCTCGCTGCCTGTGCCGGGCTGTCGGTGCTGCTCTACGACAGCCGCCAGGGGGCGGCGCTGCAAgcg   <  1:2527998/150‑1 (MQ=255)
                                                                                                                                                gAGCACCTGGGAAAATGAGCGAGCCATCGATCGTTCTCATCGGTATCGTCGGTACCGGTGCCATGGGCCAGGGCATCGCCCAGCTCGCTGCCTGTGCCGGGCTGTCGGTGCTGCTCTACGACAGCCGCCAGGGGGCGGCGCTGCAAgcgc  >  2:11098295/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GCGCAGATCAAGGAAGTGGTCCTGGCCGGCGCCGACCTGCCGCTGGACCAGGCCCTGGCGCTGGAGCGCAAGGCCTTCCAGTTGCTGTTCGACAGCCACGACCAGAAGGAGGGCATGCGTGCCTTCCTGGAAAAACGCACAGCGGAGTACCTGGGAAAATGAGCGAGCCATCGATCGTTCTCATCGGTATCGTCGGTACCGGTGCCATGGGCCAGGGCATCGCCCAGCTCGCTGCCTGTGCCGGGCTGTCGGTGCTGCTCTACGACAGCCGCCAGGGGGCGGCGCTGCAAGCGC  >  NC_008463/1533119‑1533412

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑