Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_008463	2,545,609	T→C	I509I (ATT→ATC)	tssI →	type VI secretion system tip protein TssI/VgrG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_008463	2,545,609	0	T	C	100.0%	115.7 / NA	65	I509I (ATT→ATC)	tssI	type VI secretion system tip protein TssI/VgrG
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (31/34);  total (31/34)

CTACGAGCTGCCGGCGAACGCTACCCAGAGCGGCACCAAGAGCCGTTCGAGCAAGGGCGGCACGCCGGCCAACTTCAACGAGATCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATTGAGGTTGAGAACGACGAGAGCCATCGGGTCGGCCACGACCGGACCAAGACCATTGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGAC  >  NC_008463/2545462‑2545755                                                                                                                                                    |                                                                                                                                                   cTACGAGCTGCCGGCGAACGCTACCCAGAGCGGCACCAAGAGCCGTTCGAGCAAGGGCGGCACGCCGGCCAACTTCAACGAGATCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGa                                                                                                                                                  >  1:7320165/1‑150 (MQ=17) cTACGAGCTGCCGGCGAACGCTACCCAGAGCGGCACCAAGAGCCGTTCGAGCAAGGGCGGCACGCCGGCCAACTTCAACGAGATCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGa                                                                                                                                                  <  2:2057078/150‑1 (MQ=17)  tACGAGCTGCCGGCGAACGCTACCCAGAGCGGCACCAAGAGCCGTTCGAGCAAGGGCGGCACGCCGGCCAACTTCAACGAGATCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGAg                                                                                                                                                 <  1:7634732/150‑1 (MQ=17)   aCGAGCTGCCGGCGAACGCTACCCAGAGCGGCACCAAGAGCCGTTCGAGCAAGGGCGGCACGCCGGCCAACTTCAACGAGATCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGAgg                                                                                                                                                <  2:9479342/150‑1 (MQ=17)   aCGAGCTGCCGGCGAACGCTACCCAGAGCGGCACCAAGAGCCGTTCGAGCAAGGGCGGCACGCCGGCCAACTTCAACGAGATCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGAgg                                                                                                                                                <  2:145498/150‑1 (MQ=17)   aCGAGCTGCCGGCGAACGCTACCCAGAGCGGCACCAAGAGCCGTTCGAGCAAGGGCGGCACGCCGGCCAACTTCAACGAGATCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGAgg                                                                                                                                                >  2:2156587/1‑150 (MQ=17)    cGAGCTGCCGGCGAACGCTACCCAGAGCGGCACCAAGAGCCGTTCGAGCAAGGGCGGCACGCCGGCCAACTTCAACGAGATCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGAGGt                                                                                                                                               <  1:6990602/150‑1 (MQ=17)    cGAGCTGCCGGCGAACGCTACCCAGAGCGGCACCAAGAGCCGTTCGAGCAAGGGCGGCACGCCGGCCAACTTCAACGAGATCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGAGGt                                                                                                                                               >  1:9186684/1‑150 (MQ=17)    cGAGCTGCCGGCGAACGCTACCCAGAGCGGCACCAAGAGCCGTTCGAGCAAGGGCGGCACGCCGGCCAACTTCAACGAGATCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGAGGt                                                                                                                                               <  2:199411/150‑1 (MQ=17)      aGCTGCCGGCGAACGCTACCCAGAGCGGCACCAAGAGCCGTTCGAGCAAGGGCGGCACGCCGGCCAACTTCAACGAGATCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGAGGtcg                                                                                                                                             <  1:6551761/150‑3 (MQ=11)      aGCTGCCGGCGAACGCTACCCAGAGCGGCACCAAGAGCCGTTCGAGCAAGGGCGGCACGCCGGCCAACTTCAACGAGATCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGAGGtcg                                                                                                                                             >  1:9562572/1‑148 (MQ=11)      aGCTGCCGGCGAACGCTACCCAGAGCGGCACCAAGAGCCGTTCGAGCAAGGGCGGCACGCCGGCCAACTTCAACGAGATCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGAGGtcg                                                                                                                                             >  1:9930318/1‑148 (MQ=11)      aGCTGCCGGCGAACGCTACCCAGAGCGGCACCAAGAGCCGTTCGAGCAAGGGCGGCACGCCGGCCAACTTCAACGAGATCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGAGGtcg                                                                                                                                             <  1:2075103/150‑3 (MQ=11)      aGCTGCCGGCGAACGCTACCCAGAGCGGCACCAAGAGCCGTTCGAGCAAGGGCGGCACGCCGGCCAACTTCAACGAGATCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGAGGtcg                                                                                                                                             <  2:7084939/150‑3 (MQ=11)      aGCTGCCGGCGAACGCTACCCAGAGCGGCACCAAGAGCCGTTCGAGCAAGGGCGGCACGCCGGCCAACTTCAACGAGATCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGAGGtcg                                                                                                                                             >  2:7875747/1‑148 (MQ=11)      aGCTGCCGGCGAACGCTACCCAGAGCGGCACCAAGAGCCGTTCGAGCAAGGGCGGCACGCCGGCCAACTTCAACGAGATCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGAGGtcg                                                                                                                                             >  2:9174881/1‑148 (MQ=11)      aGCTGCCGGCGAACGCTACCCAGAGCGGCACCAAGAGCCGTTCGAGCAAGGGCGGCACGCCGGCCAACTTCAACGAGATCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGAGGtcg                                                                                                                                             >  2:9444271/1‑148 (MQ=11)                                                                                                                                  cGAGAGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGc                 >  1:9898140/1‑150 (MQ=11)                                                                                                                                  cGAGAGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGc                 >  1:8850756/1‑150 (MQ=11)                                                                                                                                  cGAGAGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGc                 >  1:10907478/1‑150 (MQ=11)                                                                                                                                   gagaGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGcc                >  2:5648601/1‑150 (MQ=11)                                                                                                                                   gagaGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGcc                <  2:6736568/150‑1 (MQ=11)                                                                                                                                   gagaGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGcc                <  2:9990893/150‑1 (MQ=11)                                                                                                                                   gagaGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGcc                <  1:6654750/150‑1 (MQ=11)                                                                                                                                   gagaGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGcc                <  1:10311566/150‑1 (MQ=11)                                                                                                                                   gagaGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGcc                >  1:10002771/1‑150 (MQ=11)                                                                                                                                    agagGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCa               >  1:7302254/1‑150 (MQ=11)                                                                                                                                    agagGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCa               >  1:7529434/1‑150 (MQ=11)                                                                                                                                    agagGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCa               >  2:2362064/1‑150 (MQ=11)                                                                                                                                     gagGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCaa              >  2:9988300/1‑150 (MQ=11)                                                                                                                                     gagGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCaa              <  2:3730668/150‑1 (MQ=11)                                                                                                                                     gagGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCaa              >  2:10999459/1‑150 (MQ=11)                                                                                                                                      agGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAAc             <  1:1204656/150‑1 (MQ=11)                                                                                                                                      agGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAAc             <  2:5373551/150‑1 (MQ=11)                                                                                                                                      agGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAAc             <  1:2319180/150‑1 (MQ=11)                                                                                                                                       ggAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGTCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAAcc            <  2:3163764/150‑1 (MQ=11)                                                                                                                                       ggAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAAcc            <  1:1867744/150‑1 (MQ=11)                                                                                                                                       ggAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAAcc            >  1:4750786/1‑150 (MQ=11)                                                                                                                                       ggAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAAcc            >  2:2282567/1‑150 (MQ=11)                                                                                                                                        gAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCg           >  1:2551668/1‑150 (MQ=11)                                                                                                                                        gAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCg           >  1:2539800/1‑150 (MQ=11)                                                                                                                                        gAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCg           >  1:2539907/1‑150 (MQ=11)                                                                                                                                         accccggACATCGAGGTCGAGAACGACGCGAGCCCCTGGGTCTGCCACGACCGGACCAAGACCATCGACCCCGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCgc          <  2:4812312/145‑1 (MQ=11)                                                                                                                                         aaCCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCgc          <  1:4425501/150‑1 (MQ=11)                                                                                                                                         aaCCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCgc          >  2:3338326/1‑150 (MQ=11)                                                                                                                                          aCCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACACCGAGACCATCACCGTGCATGCCAACCgca         <  2:3177704/150‑1 (MQ=11)                                                                                                                                          aCCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCgca         >  1:10595037/1‑150 (MQ=11)                                                                                                                                          aCCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGAGCATGCCAACCgca         >  2:9523970/1‑150 (MQ=11)                                                                                                                                            cAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCgcagc       >  1:6525341/1‑150 (MQ=11)                                                                                                                                            cAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCgcagc       <  1:2054612/150‑1 (MQ=11)                                                                                                                                            cAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCgcagc       >  2:3188457/1‑150 (MQ=11)                                                                                                                                             aGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCgcagca      >  1:3364310/1‑150 (MQ=11)                                                                                                                                              ggACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCaa     >  1:1155901/1‑150 (MQ=11)                                                                                                                                              ggACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCaa     <  1:11000406/150‑1 (MQ=11)                                                                                                                                              ggACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCaa     <  1:10973842/150‑1 (MQ=11)                                                                                                                                              ggACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCaa     <  1:6442457/150‑1 (MQ=11)                                                                                                                                              ggACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCaa     <  1:4631526/150‑1 (MQ=11)                                                                                                                                               gACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAg    <  2:8751122/150‑1 (MQ=14)                                                                                                                                               gACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAg    <  1:8809185/150‑1 (MQ=14)                                                                                                                                               gACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAg    <  1:8809158/150‑1 (MQ=14)                                                                                                                                                aCATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGa   <  2:2344020/150‑1 (MQ=14)                                                                                                                                                aCATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGa   >  1:2194319/1‑150 (MQ=14)                                                                                                                                                aCATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGa   <  2:1801859/150‑1 (MQ=14)                                                                                                                                                aCATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGa   <  1:4671851/150‑1 (MQ=14)                                                                                                                                                 cATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGAc  <  1:3152275/150‑1 (MQ=14)                                                                                                                                                    |                                                                                                                                                   CTACGAGCTGCCGGCGAACGCTACCCAGAGCGGCACCAAGAGCCGTTCGAGCAAGGGCGGCACGCCGGCCAACTTCAACGAGATCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATTGAGGTTGAGAACGACGAGAGCCATCGGGTCGGCCACGACCGGACCAAGACCATTGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGAC  >  NC_008463/2545462‑2545755

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑