Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 2,649,107 A→G N212S (AAC→AGC)  PA14_RS12475 → TonB‑dependent receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084632,649,1070AG99.8% 1764.0 / NA 470N212S (AAC→AGC) PA14_RS12475TonB‑dependent receptor
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (247/222);  minor base T (1/0);  total (248/222)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 470 total aligned reads displayed.

GCAGCAGCGGCGCGGTCGACCTGCGCGATGCACTGGTCAAACTGCTGCCCTCGCTGACCCGCCAGGCCCAGCCGTTCAACGCCTCGGCGTTGACCCACACGCAGTCGCTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAACGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGGAGAAGGGCTCCACCGGCGTCGATCTGGACACCATCCCGATCAACGCCATCGAGCGGGTCGAGGTGCTGCGCGACGGTGCTTCGGCACAGTATGGTT  >  NC_008463/2648960‑2649253
                                                                                                                                                   |                                                                                                                                                  
 cagcagCGGCGCGGTCGACCTGCGCGATGCACTGGTCAAACTGCTGCCCTCGCTGACCCGCCAGGCCCAGCCGTTCAACGCCTCGGCGTTGACCCACACGCAGTCGCTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCgg                                                                                                                                                 >  2:2337693/1‑150 (MQ=255)
   gcagcGGCGCGGTCGACCTGCGCGATGCACTGGTCAAACTGCTGCCCTCGCTGACCCGCCAGGCCCAGCCGTTCAACGCCTCGGCGGTGACCCACACGCAGTCGCTGCGCGGGCTGAGCCCCAACCAGGTGCTGGTGCTGGTGAGCGGCa                                                                                                                                               >  2:1704546/1‑150 (MQ=255)
   gcagcGGCGCGGTCGACCTGCGCGATGCACTGGTCAAACTGCTGCCCTCGCTGACCCGCCAGGCCCAGCCGTGCCACGCCTCGGCGTGGACCCACACGCAGTCGCGGCGCGGGCGGAGCCCCAACCAAGTGCTGGTGCTGGTGAGCGGCa                                                                                                                                               >  1:5172073/1‑150 (MQ=255)
      gcggcgCGGTCGACCTGCGCGATGCACTGGTCAAACTGCTGCCCTCGCTGACCCGCCAGGCCCAGCCGTTCAACGCCTCGGCGTTGACCCACACGCAGTCGCTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGc                                                                                                                                            >  2:1267824/1‑150 (MQ=255)
      gcggcgCGGTCGACCTGCGCGATGCACTGGTCAAACTGCTGCCCTCGCTGACCCGCCAGGCCCAGCCGTTCAACGCCTCGGCGGTGACCCACACGCAGTCGCTGCGCGGGCTGTGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGc                                                                                                                                            >  1:4520046/1‑150 (MQ=255)
        ggcgCGGTCGACCTGCGCGATGCACTGGTCAAACTGCTGCCCTCGCTGACCCGCCAGGCCCAGCCGTTCAACGCCTCGGCGTTGACCCACACGCAGTCGCTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGt                                                                                                                                          >  2:4864721/1‑150 (MQ=255)
        ggcgCGGTCGACCTGCGCGATGCACTGGTCAAACTGCTGCCCTCGCTGACCCGCCAGGCCCAGCCGTTCAACGCCTCGGCGTTGACCCACACGCAGTCGCTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGt                                                                                                                                          <  1:870316/150‑1 (MQ=255)
        ggcgCGGTCGACCTGCGCGATGCACTGGTCAAACTGCTGCCCTCGCTGACCCGCCAGGCCCAGCCGTTCAACGCCTCGGCGTTGACCCACACGCAGTCGCTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGt                                                                                                                                          <  1:6486497/150‑1 (MQ=255)
         gcgcGGTCGACCTGCGCGATGCACTGGTCAAACTGCTGCCCTCGCTGACCCGCCAGGCCCAGCCGTTCAACGCCTCGGCGTTGACCCACACGCAGTCGCTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCACGCGTc                                                                                                                                         >  2:5974123/1‑150 (MQ=255)
            cgGTCGACCTGCGCGATGCACTGGTCAAACTGCTGCCCTCGCTGACCCGCCAGGCCCAGCCGTTCAACGCCTCGGCGTTGACCCACACGCAGTCGCTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGcc                                                                                                                                      <  1:5656506/150‑1 (MQ=255)
            cgGTCGACCTGCGCGATGCACTGGTCAAACTGCTGCCCTCGCTGACCCGCCAGGCCCAGCCGTTCAACGCCTCGGCGTTGACCCACACGCAGTCGCTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGcc                                                                                                                                      <  1:8063017/150‑1 (MQ=255)
             ggTCGACCTGCGCGATGCACTGGTCAAACTGCTGCCCTCGCTGACCCGCCAGGCCCAGCCGTTCAACGCCTCGGCGTTGACCCACACGCAGTCGCTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCa                                                                                                                                     <  1:10023149/150‑1 (MQ=255)
             ggTCGACCTGCGCGATGCACTGGTCAAACTGCTGCCCTCGCTGACCCGCCAGGCCCAGCCGTTCAACGCCTCGGCGTTGACCCACACGCAGTCGCTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCa                                                                                                                                     >  2:9533730/1‑150 (MQ=255)
                cGACCTGCGCGATGCACTGGTCAAACTGCTGCCCTCGCTGACCCGCCAGGCCCAGCCGTTCAACGCCTCGGCGTTGACCCACACGCAGTCGCTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGa                                                                                                                                  <  2:3708598/150‑1 (MQ=255)
                 gACCTGCGCGATGCACTGGTCAAACTGCTGCCCTCGCTGACCCGCCAGGCCCAGCCGTTCAACGCCTCGGCGTTGACCCACACGCAGTCGCTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGaa                                                                                                                                 >  2:10389719/1‑150 (MQ=255)
                  aCCTGCGCGATGCACTGGTCAAACTGCTGCCCTCGCTGACCCGCCAGGCCCAGCCGTTCAACGCCTCGGCGTTGACCCACACGCAGTCGCTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGaaa                                                                                                                                <  2:2132730/150‑1 (MQ=255)
                  aCCTGCGCGATGCACTGGTCAAACTGCTGCCCTCGCTGACCCGCCAGGCCCAGCCGTTCAACGCCTCGGCGTTGACCCACACGCAGTCGCTGCGCGGGCTGAGCCCCAACCAAGTGCTGGTGCTGGTGAGCGGCCAGCGTCGCCACGaaa                                                                                                                                >  1:4346927/1‑150 (MQ=255)
                   ccTGCGCGATGCACTGGTCAAACTGCTGCCCTCGCTGACCCGCCAGGCCCAGCCGTTCAACGCCTCGGCGTTGACCCACACGCAGTCGCTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAAc                                                                                                                               >  2:9027647/1‑150 (MQ=255)
                    cTGCGCGATGCACTGGTCAAACTGCTGCCCTCGCTGACCCGCCAGGCCCAGCCGTTCAACGCCTCGGCGTTGACCCACACGCAGTCGCAGCGCGGGCGGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACg                                                                                                                              >  1:7332026/1‑150 (MQ=255)
                      gcgcGATGCACTGGTCAAACTGCTGCCCTCGCTGACCCGCCAGGCCCAGCCGTTCAACGCCTCGGCGTTGACCCACACGCAGTCGCTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGc                                                                                                                            >  1:7788097/1‑150 (MQ=255)
                      gcgcGATGCACTGGTCAAACTGCGGCCCTCGCTGACCCGCCAGGCCCAGCCGTTCAACGCCTCGGCGTTGACCCACACGCAGTCGCTGCGCGGGCTGAGCCCCAACCCTGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGc                                                                                                                            <  2:2769818/150‑1 (MQ=255)
                        gcgATGCACTGGTCAAACTGCTGCCCTCGCTGACCCGCCAGGCCCAGCCGTTCAACGCCTCGGCGTTGACCCACACGCAGTCGCTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCa                                                                                                                          <  2:1452462/150‑1 (MQ=255)
                         cgATGCACTGGTCAAACTGCTGCCCTCGCTGACCCGCCAGGCCCAGCCGTTCAACGCCTCGGCGTTGACCCACACGCAGTCGCTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCaa                                                                                                                         <  1:1998957/150‑1 (MQ=255)
                          gATGCACTGGTCAAACTGCTGCCCTCGCTGACCCGCCAGGCCCAGCCGTTCAACGCCTCGGCGTTGACCCACACGCAGTCGCTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAAt                                                                                                                        <  2:4426766/150‑1 (MQ=255)
                           aTGCACTGGTCAAACTGCTGCCCTCGCTGACCCGCCAGGCCCAGCCGTTCAACGCCTCGGCGTTGACCCACACGCAGTCGCTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAAtc                                                                                                                       <  2:7075110/150‑1 (MQ=255)
                            tGCACTGGTCAAACTGCTGCCCTCGCTGACCCGCCAGGCCCAGCCGTTCAACGCCTCGGCGTTGACCCACACGCAGTCGCTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAAtct                                                                                                                      <  1:4796340/150‑1 (MQ=255)
                               aCTGGTCAAACTGCTGCCCTCGCTGACCCGCCAGGCCCAGCCGTTCAACGCCTCGGCGTTGACCCACACGCAGTCGCTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCaa                                                                                                                   >  2:3175328/1‑150 (MQ=255)
                                cTGGTCAAACTGCTGCCCTCGCTGACCCGCCAGGCCCAGCCGTTCAACGCCACGGCGTTGACCCACACGAAGTCGCTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAAGCTCAat                                                                                                                  >  2:9840868/1‑150 (MQ=255)
                                cTGGTCAAACTGCTGCCCTCGCTGACCCGCCAGGCCCAGCCGTGCAACGCCACGGCGTTGACCCACAACCAGTCGCGGCGCGGGGGGTGCCCCAACCCAGTGCTGGTGCTGGTGTGCGGCAAGCGTCGCCACGAAACCGCCAAAATCAat                                                                                                                  >  1:7477577/1‑150 (MQ=255)
                                 tGGTCAAACTGCTGCCCTCGCTGACCCGCCAGGCCCAGCCGTTCAACGCCTCGGCGTTGACCCACACGCAGTCGCTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAata                                                                                                                 <  2:5023360/150‑1 (MQ=255)
                                      aaaCTGCTGCCCTCGCTGACCCGCCAGGCCCAGCCGTTCAACGCCTCGGCGTTGACCCACACGCAGTCGCTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGc                                                                                                            <  1:6880844/150‑1 (MQ=255)
                                           gctgcCCTCGCTGACCCGCCAGGCCCAGCCGTTCAACGCCTCGGCGTTGACCCACACGCAGTCGCTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCggggg                                                                                                       >  1:5792699/1‑150 (MQ=255)
                                             tgcCCTCGCTGACCCGCCAGGCCCAGCCGTTCAACGCCTCGGCGTTGACCCACACGCAGTCGCTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGc                                                                                                     <  1:8846751/150‑1 (MQ=255)
                                              gcCCTCGCTGACCCGCCAGGCCCAGCCGTTCAACGCCTCGGCGTTGACCCACACGCAGTCGCTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCt                                                                                                    <  2:10952969/150‑1 (MQ=255)
                                                  tCGCTGACCCGCCAGGCCCAGCCGTTCAACGCCTCGGCGTTGACCCACACGCAGTCGCTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGgag                                                                                                >  2:1408767/1‑150 (MQ=255)
                                                  tCGCTGACCCGCCAGGCCCAGCCGTTCAACGCCTCGGCGTTGACCCACACGCAGTCGCTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGgag                                                                                                >  2:8645820/1‑150 (MQ=255)
                                                    gCTGACCCGCCAGGCCCAGCCGTTCAACGCCTCGGCGTTGACCCACACGCAGTCGCTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGGAGaa                                                                                              <  1:10715398/150‑1 (MQ=255)
                                                     cTGACCCGCCAGGCCCAGCCGTTCAACGCCTCGGCGTTGACCCACACGCAGTCGCTGCGCGGGCTGAGCCCCAACCCTGTGCTGGTGCGGGTGAGCGGCAAGCGTCGACACGAAAAGGCCAATCTCACTATCAGCGGGGGGCGGGAGAAg                                                                                             >  1:3956399/1‑150 (MQ=255)
                                                      tGACCCGCCAGGCCCAGCCGTTCAACGCCTCGGCGTTGACCCACACGCAGTCGCGGCGCGGGCTGTGCCCCAACCAAGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGGAGAAgg                                                                                            >  1:5489369/1‑150 (MQ=255)
                                                          ccGCCAGGCCCAGCCGTTCAACGCCTCGGCGTTGACCCACACGCAGTCGCTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGGAGAAGGGCTc                                                                                        <  1:2216335/150‑1 (MQ=255)
                                                           cGCCAGGCCCAGCCGTTCAACGCCTCGGCGTTGACCCACACGCAGTCGCTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCGGGAGAAGGGCTcc                                                                                       >  2:3556539/1‑150 (MQ=255)
                                                             ccAGGCCCAGCCGTTCAACGCCTCGGCGTTGACCCACACGCAGTCGCTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGGAGAAGGGCTCCAc                                                                                     >  2:3373212/1‑150 (MQ=255)
                                                              cAGGCCCAGCCGTTCCACGCCTCGGCGTTGACCCACACGCAGTCGCTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGGAGAAGGGCTCCAcc                                                                                    >  1:2380867/1‑150 (MQ=255)
                                                              cAGGCCCAGCCGTTCAACGCCTCGGCGTTGACCCACACGCAGTCGCTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGGAGAAGGGCTCCAcc                                                                                    >  2:5404181/1‑150 (MQ=255)
                                                               aGGCCCAGCCGTTCAACGCCTCGGCGTTGACCCACACGCAGTCGCTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGGAGAAGGGCTCCACCg                                                                                   <  1:7684607/150‑1 (MQ=255)
                                                                ggCCCAGCCGTTCAACGCCTCGGCGTTGACCCACACGCAGTCGCTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGGAGAAGGGCTCCACCgg                                                                                  <  1:6346371/150‑1 (MQ=255)
                                                                      gCCGTTCAACGCCTCGGCGTTGACCCACACGCAGTCGCTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGGAGAAGGGCTCCACCGGCGTCGa                                                                            >  2:2239387/1‑150 (MQ=255)
                                                                      gCCGTTCAACGCCTCGGCGTTGACCCACACGCAGTCGCTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGGAGAAGGGCTCCACCGGCGTCGa                                                                            <  2:664475/150‑1 (MQ=255)
                                                                        cGTTCAACGCCTCGGCGTTGACCCACACGCAGTCGCTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGACACGGCCAATCTCACTATCAGCGGGGGGCTGGAGAAGGGCTCCACCGGCGTCGATc                                                                          <  1:5956097/150‑1 (MQ=255)
                                                                        cGTTCAACGCCTCGGCGTTGACCCACACGCAGTCGCTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGGAGAAGGGCTCCACCGGCGTCGATc                                                                          >  2:9300960/1‑150 (MQ=255)
                                                                          ttCAACGCCTCGGCGTTGACCCACACGCAGTCGCTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGGAGAAGGGCTCCACCGGCGTCGATCTg                                                                        >  1:1132644/1‑150 (MQ=255)
                                                                               cGCCTCGGCGTTGACCCACACGCAGTCGCTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGGAGAAGGGCTCCACCGGCGTCGATCTGGacac                                                                   <  2:845626/150‑1 (MQ=255)
                                                                                gCCTCGGCGTTGACCCACACGCAGTCGCTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGGAGAAGGGCTCCACCGGCGTCGATCTGGACAcc                                                                  >  1:10175216/1‑150 (MQ=255)
                                                                                gCCTCGGCGTTGACCCACACGCAGTCGCTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGGAGAAGGGCTCCACCGGCGTCGATCTGGACAcc                                                                  <  1:8615331/150‑1 (MQ=255)
                                                                                  cTCGGCGTTGACCCACACGCAGTCGCTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGGAGAAGGGCTCCACCGGCGTCGATCTGGACACCAt                                                                >  1:7097241/1‑150 (MQ=255)
                                                                                   tCGGCGTTGACCCACACGCAGTCGCTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGGAGAAGGGCTCCACCGGCGTCGAGCTGGACACCATc                                                               >  2:8179313/1‑150 (MQ=255)
                                                                                     ggCGTTGACCCACACGCAGTCGCTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGGAGAAGGGCTCCACCGGCGTCGATCTGGACACCATccc                                                             >  1:8344862/1‑150 (MQ=255)
                                                                                        gTTGACCCACACGCAGTCGCTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGGAGAAGGGCTCCACCGGCGTCGATCTGGACACCATCCCGAt                                                          >  2:7220481/1‑150 (MQ=255)
                                                                                        gTTGACCCACACGCAGTCGCTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGGAGAAGGGCTCCACCGGCGTCGATCTGGACACCATCCCGAt                                                          <  1:2522903/150‑1 (MQ=255)
                                                                                            aCCCACACGCAGTCGCTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGGAGAAGGGCTCCACCGGCGTCGATCTGGACACCATCCCGATCAAc                                                      <  2:11102089/150‑1 (MQ=255)
                                                                                            aCCCACACGCAGTCGCTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGGAGAAGGGCTCCACCGGCGTCGATCTGGACACCATCCCGATCAAc                                                      >  2:7742694/1‑150 (MQ=255)
                                                                                                   cGCAGTCGCTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGGAGAAGGGCTCCACCGGCGTCGATCTGGACACCATCCCGATCAACGCCATCg                                               <  2:8400359/150‑1 (MQ=255)
                                                                                                    gCAGTCGCTGCGCGGGCGGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGGAGAAGGGCTCCACCGGCGTCGATCTGGACACCATCCCGATCAACGCCATCGa                                              >  2:10253988/1‑150 (MQ=255)
                                                                                                        tCGCTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGGAGAAGGGCTCCACCGGCGTCGATCTGGACACCATCCCGATCAACGCCATCGAGCgg                                          >  1:4187995/1‑150 (MQ=255)
                                                                                                         cGCTGCGCGGGCTGAGCCCCAACCCTGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGGAGAAGGGCTCCACCGGCGTCGATCTGGACACCATCCCGATCAACGCCATCGAGCggg                                         <  2:2039932/150‑1 (MQ=255)
                                                                                                          gcagcgcGGGCTGCGCCCCCACCATGGGCGGGTGCTGGGGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAAGCTCACCGGGGGGCTGGAGAAGGGCTCCACCGGCGTCGATCTGGACACCAGCCCGAACAACGCCATCGAGCGGGt                                        <  2:10677958/147‑1 (MQ=255)
                                                                                                           cTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGGAGAAGGGCTCCACCGGCGTCGATCTGGACACCATCCCGATCAACGCCATCGAGCGGGTc                                       >  2:10455576/1‑150 (MQ=255)
                                                                                                            tGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGGAGAAGGGCTCCACCGGCGTCGATCTGGACACCATCCCGATCAACGCCATCGAGCGGGTCg                                      >  2:5572981/1‑150 (MQ=255)
                                                                                                              cgcgGGCTGAGCCCCAACCATGTGCGGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGGAGAAGGGCTCCACCGGCGTCGATCTGGACACCATCCCGATCAACGCCATCGAGCGGGTCGAg                                    >  1:3361260/1‑150 (MQ=255)
                                                                                                                 gggCTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGGAGAAGGGCTCCACCGGCGTCGATCTGGACACCATCCCGATCAACGCCATCGAGCGGGTCGAGGtg                                 >  1:3214567/1‑150 (MQ=255)
                                                                                                                   gCTGAGCCCCCACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGGAGAAGGGCTCCACCGGCGTCGATCTGGACACCATCCCGATCAACGCCATCGAGCGGGTCGAGGtgct                               <  1:1917325/150‑1 (MQ=255)
                                                                                                                   gCTGAGCCCCAACCCTGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGGAGAAGGGCTCCACCGGCGTCGATCTGGACACCATCCCGATCAACGCCATCGAGCGGGTCGAGGtgct                               <  1:10386111/150‑1 (MQ=255)
                                                                                                                    cTGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGGAGAAGGGCTCCACCGGCGTCGATCTGGACACCATCCCGATCAACGCCATCGAGCGGGTCGAGGtgctg                              <  2:826321/150‑1 (MQ=255)
                                                                                                                     tGAGCCCCAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGGAGAAGGGCTCCACCGGCGTCGATCTGGACACCATCCCGATAAACGCCATCGAGCGGGTCGAGGtgctgc                             <  1:10968014/150‑1 (MQ=255)
                                                                                                                       aGCCCCACCCATGTGCTGGTGCGGGTGAGCGGCAAGCGTCGCCACGAAACGGCCACTCTCAATATCAGCGGGGGGCTGGAGAAGGGCTCCACCGGCGTCGATCTGGACACCATCCCGATCAACGCCATCGAGCGGGTCGAGGTGCTgcgc                           <  2:2529711/150‑1 (MQ=255)
                                                                                                                         ccccAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGGAGACGGGCTCCACCGGCGACGATCTGGACACCAGCCCGATCAACGCCAACGAGCGGGTCGAGGTGCTGCGCGa                         >  2:6481610/1‑150 (MQ=255)
                                                                                                                          cccAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGGAGAAGGGCTCCACCGGCGTCGATCTGGACACCATCCCGATCAACGCCATCGAGCGGGTCGAGGTGCTGCGCGAc                        >  1:931600/1‑150 (MQ=255)
                                                                                                                           ccAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGGAGAAGGGCTCCACCGGCGTCGATCTGGACACCATCCCGATCAACGCCATCGAGCGGGTCGAGGTGCTGCGCGACg                       >  2:6123240/1‑150 (MQ=255)
                                                                                                                           ccAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGGAGAAGGGCTCCACCGGCGTCGATCTGGACACCATCCCGATCAACGCCATCGAGCGGGTCGAGGTGCTGCGCGACg                       >  2:7493097/1‑150 (MQ=255)
                                                                                                                            cAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGGAGAAGGGCTCCACCGGCGTCGATCTGGACACCATCCCGATCAACGCCATCGAGCGGGTCGAGGTGCTGCGCGACgg                      <  2:9642063/150‑1 (MQ=255)
                                                                                                                            cAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGGAGAAGGGCTCCACCGGCGTCGATCTGGACACCATCCCGATCAACGCCATCGAGCGGGTCGAGGTGCTGCGCGACgg                      >  2:301029/1‑150 (MQ=255)
                                                                                                                            cAACCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGGAGAAGGGCTCCACCGGCGTCGATCTGGACACCATCCCGATCAACGCCATCGAGCGGGTCGAGGTGCTGCGCGACgg                      <  2:5717382/150‑1 (MQ=255)
                                                                                                                             aaCCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGGAGAAGGGCTCCACCGGCGTCGATCTGGACACCATCCCGATCAACGCCATCGAGCGGGTCGAGGTGCTGCGCGACGGt                     >  1:3825521/1‑150 (MQ=255)
                                                                                                                              aCCATGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGGAGAAGGGCTCCACCGGCGTCGATCTGGACACCATCCCGATCAACGCCATCGAGCGGGTCGAGGTGCTGCGCGACGGTg                    >  1:10606671/1‑150 (MQ=255)
                                                                                                                                 aTGTGCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGGAGAAGGGCTCCACCGGCGTCGATCTGGACACCATCCCGATCAACGCCATCGAGCGGGTCGAGGTGCTGCGCGACGGTGCtt                 >  1:9109166/1‑150 (MQ=255)
                                                                                                                                  tgtgCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGGAGAAGGGCTCCACCGGCGTCGATCTGGACACCATCCCGATCAACGCCATCGAGCGGGTCGAGGTGCTGCGCGACGGTGCTTc                >  1:3487977/1‑150 (MQ=255)
                                                                                                                                  tgtgCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGGAGAAGGGCTCCACCGGCGTCGATCTGGACACCATCCCGATCAACGCCATCGAGCGGGTCGAGGTGCTGCGCGACGGTGCTTc                >  1:1638266/1‑150 (MQ=255)
                                                                                                                                  tgtgCTGGTGCTGGTGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGGAGAAGGGCTCCACCGGCGTCGATCTGGACACCATCCCGATCAACGCCATCGAGCGGGTCGAGGTGCTGCGCGACGGTGCTTc                <  1:9975761/150‑1 (MQ=255)
                                                                                                                                   gtgctggtgctgGTGAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGGAGAAGGGCTCCACCGGCGTCGATCTGGACACCATCCCGATCAACGCCATCGAGCGGGTCGAGGTGCTGCGCGACGGTGCTTCg               <  1:6247166/150‑1 (MQ=255)
                                                                                                                                       tggtgctggtgAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGGAGAAGGGCTCCACCGGCGTCGATCTGGACACCATCCCGATCAACGCCATCGAGCGGGTCGAGGTGCTGCGCGACGGTGCTTCGGcac           >  2:4037028/1‑150 (MQ=255)
                                                                                                                                        ggtgctggtgAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGGAGAAGGGCTCCACCGGCGTCGATCTGGACACCATCCCGATCAACGCCATCGAGCGGGTCGAGGTGCTGCGCGACGGTGCTTCGGcaca          <  2:6982233/150‑1 (MQ=255)
                                                                                                                                         gtgctggtgAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGGAGAAGGGCTCCACCGGCGTCGATCTGGACACCATCCCGATCAACGCCATCGAGCGGGTCGAGGTGCTGCGCGACGGTGCTTCGGCACAg         >  2:7896281/1‑150 (MQ=255)
                                                                                                                                           gctggtgAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGGAGAAGGGCTCCACCGGCGTCGATCTGGACACCATCCCGATCAACGCCATCGAGCGGGTCGAGGTGCTGCGCGACGGTGCTTCGGCACAGTa       <  1:1804080/150‑1 (MQ=255)
                                                                                                                                           gctggtgAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGGAGAAGGGCTCCACCGGCGTCGATCTGGACACCATCCCGATCAACGCCATCGAGCGGGTCGAGGTGCTGCGCGACGGTGCTTCGGCACAGTa       >  1:1400920/1‑150 (MQ=255)
                                                                                                                                            ctggtgAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGGAGAAGGGCTCCACCGGCGTCGATCTGGACACCATCCCGATCAACGCCATCGAGCGGGTCGAGGTGCTGCGCGACGGTGCTTCGGCACAGTAt      <  1:2710951/150‑1 (MQ=255)
                                                                                                                                             tggtgAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGGAGAAGGGCTCCACCGGCGTCGATCTGGACACCATCCCGATCAACGCCATCGAGCGGGTCGAGGTGCTGCGCGACGGTGCTTCGGCACAGTATg     <  2:7944325/150‑1 (MQ=255)
                                                                                                                                              ggtgAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGGAGAAGGGCTCCACCGGCGTCGATCTGGACACCATCCCGATCAACGCCATCGAGCGGGTCGAGGTGCTGCGCGACGGTGCTTCGGCACAGTATgg    >  1:9634202/1‑150 (MQ=255)
                                                                                                                                               gtgAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGGAGAAGGGCTCCACCGGCGTCGATCTGGACACCATCCCGATCAACGCCATCGAGCGGGTCGAGGTGCTGCGCGACGGTGCTTCGGCACAGTATGGt   >  2:10069287/1‑150 (MQ=255)
                                                                                                                                               gtgAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGGAGAAGGGCTCCACCGGCGTCGATCTGGACACCATCCCGATCAACGCCATCGAGCGGGTCGAGGTGCTGCGCGACGGTGCTTCGGCACAGTATGGt   <  2:6830047/150‑1 (MQ=255)
                                                                                                                                                tgAGCGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGGAGAAGGGCTCCACCGGCGTCGATCTGGACACCATCCCGATCAACGCCATCGAGCGGGTCGAGGTGCTGCGCGACGGTGCTTCGGCACAGTATGGtt  <  1:8294294/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GCAGCAGCGGCGCGGTCGACCTGCGCGATGCACTGGTCAAACTGCTGCCCTCGCTGACCCGCCAGGCCCAGCCGTTCAACGCCTCGGCGTTGACCCACACGCAGTCGCTGCGCGGGCTGAGCCCCAACCATGTGCTGGTGCTGGTGAACGGCAAGCGTCGCCACGAAACGGCCAATCTCAATATCAGCGGGGGGCTGGAGAAGGGCTCCACCGGCGTCGATCTGGACACCATCCCGATCAACGCCATCGAGCGGGTCGAGGTGCTGCGCGACGGTGCTTCGGCACAGTATGGTT  >  NC_008463/2648960‑2649253

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑