Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 2,651,339 T→C F68L (TTC→CTC)  PA14_RS12480 → MFS transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084632,651,3390TC99.6% 1758.4 / NA 449F68L (TTC→CTC) PA14_RS12480MFS transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (218/229);  minor base A (1/1);  total (219/230)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 449 total aligned reads displayed.

GTGGCGATCATGCTCGGGCCCACCGGCATCCTGCTGAACACCTTCAGTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGTTCAAGGGCTGGCTGATCAACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTGTTCCTGCTGATCGGCCTGCTGGC  >  NC_008463/2651192‑2651484
                                                                                                                                                   |                                                                                                                                                 
    cGATCATGCTCGGGCCCACCGGCATCCTGCTGAACACCTTCAGTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAgg                                                                                                                                             >  1:9107170/1‑150 (MQ=255)
       tCATGCTCGGGCCCACCGGCATCCTGCTGAACACCTTCAGTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGggct                                                                                                                                          <  2:2147290/150‑1 (MQ=255)
         aTGCTCGGGCCCACCGGCATCCTGCTGAACACCTTCAGTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGggctgg                                                                                                                                        <  1:3199663/150‑1 (MQ=255)
          tGCTCGGGCCCACCGGCATCCTGCTGAACACCTTCAGTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGggctggc                                                                                                                                       <  1:7208933/150‑1 (MQ=255)
             tCGGGCCCACCGGCATCCTGCTGAACACCTTCAGTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGa                                                                                                                                    >  2:7385992/1‑150 (MQ=255)
             tCGGGCCCACCGGCATCCTGCTGAACACCTTCAGTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGa                                                                                                                                    >  2:2633735/1‑150 (MQ=255)
              cGGGCCCACCGGCATCCTGCTGAACACCTTCAGTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGAt                                                                                                                                   <  2:8375117/150‑1 (MQ=255)
               gggCCCACCGGCATCCTGCTGAACACCTTCAGTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATc                                                                                                                                  >  1:7948277/1‑150 (MQ=255)
               gggCCCACCGGCATCCTGCTGAACACCTTCAGTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATc                                                                                                                                  <  1:3661797/150‑1 (MQ=255)
                 gCCCACCGGCATCCTGCTGAACACCTTCAGTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATcga                                                                                                                                <  1:10249153/150‑3 (MQ=255)
                    cACCGGCATCCTGCTGAACACCTTCAGTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCg                                                                                                                             <  2:6247092/150‑1 (MQ=255)
                     aCCGGCATCCTGCTGAACACCTTCAGTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGt                                                                                                                            <  1:5045216/150‑1 (MQ=255)
                     aCCGGCATCCTGCTGAACACCGTCAGTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGt                                                                                                                            <  2:8669609/150‑1 (MQ=255)
                       cGGCATCCTGCCGAACACCTTCAGTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTg                                                                                                                          >  2:3522326/1‑150 (MQ=255)
                        ggCATCCTGCTGAACACCTTCAGTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCCGCCCGCTCAAGGGCTGGCTGATCGACCGTTgg                                                                                                                         >  2:5513225/1‑150 (MQ=255)
                        ggCATCCTGCTGAACACCTTCAGTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTgg                                                                                                                         >  1:9647622/1‑150 (MQ=255)
                           aTCCTGCTGAACACCTTCAGTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGgc                                                                                                                      >  1:6181151/1‑150 (MQ=255)
                           aTCCTGCTGAACACCTTCAGTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCGGGTGACGCTGTTCGGACTGGCGGTCGCCCTCACCAGCCCGCTCATGGGCTGGCTGATCGACCGGAGGGgc                                                                                                                      >  2:8528279/1‑150 (MQ=255)
                            tCCTGCTGAACACCTTCAGTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGgcg                                                                                                                     >  2:4013609/1‑150 (MQ=255)
                              ctgctgAACACCTTCAGTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTGGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGGTGGGgcgcg                                                                                                                   >  2:5773337/1‑150 (MQ=255)
                                   gAACACCTTCAGTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCgcggcg                                                                                                              >  2:3450311/1‑150 (MQ=255)
                                   gAACACCTTCAGTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCgcggcg                                                                                                              >  2:3159552/1‑150 (MQ=255)
                                    aaCACCTTCAGTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGGTGGGGCGCGcggcgg                                                                                                             >  1:4385336/1‑150 (MQ=255)
                                     acacCTTCAGTCTGTTCATCGCACCGTTGAGCGCCGAGTACGCCTGGGACCGGGCCGAGGTGTCGTTGCTGGTGACGCGGTTCGGACTGGCGGTCGCCATCACCAGCCCGCGCCAGGGCTGGCTGAGCGCCCGGTGGGGCGCGCGGCGGa                                                                                                            >  2:9062493/1‑150 (MQ=255)
                                     acacCTTCAGTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGa                                                                                                            >  2:3035250/1‑150 (MQ=255)
                                       acCTTCAGTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACa                                                                                                          <  2:944139/150‑1 (MQ=255)
                                       acCTTCAGTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACa                                                                                                          <  1:9260318/150‑1 (MQ=255)
                                          ttCAGTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATg                                                                                                       >  2:2790631/1‑150 (MQ=255)
                                              gTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCg                                                                                                   >  1:1212129/1‑150 (MQ=255)
                                               tCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCgg                                                                                                  <  1:3538395/150‑1 (MQ=255)
                                               tCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCgg                                                                                                  <  2:4377533/150‑1 (MQ=255)
                                                 tGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGc                                                                                                <  1:8181056/150‑1 (MQ=255)
                                                 tGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGc                                                                                                >  1:7606703/1‑150 (MQ=255)
                                                    tCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGa                                                                                             >  2:1066194/1‑150 (MQ=255)
                                                      aTCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGAcc                                                                                           <  2:6833162/150‑1 (MQ=255)
                                                            ccGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGcc                                                                                     >  2:3299909/1‑150 (MQ=255)
                                                             cGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGccc                                                                                    >  2:10823438/1‑150 (MQ=255)
                                                              gatgatCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCAGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCt                                                                                   >  2:2259789/1‑150 (MQ=255)
                                                              gATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCt                                                                                   >  1:771601/1‑150 (MQ=255)
                                                               aTGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTg                                                                                  >  2:1379843/1‑150 (MQ=255)
                                                               aTGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTg                                                                                  >  1:2420644/1‑150 (MQ=255)
                                                                 gAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTc                                                                                >  2:1994154/1‑150 (MQ=255)
                                                                   gcgcCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTcgc                                                                              <  1:10460510/150‑1 (MQ=255)
                                                                    cgcCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTcgct                                                                             <  1:6326313/150‑1 (MQ=255)
                                                                      ccGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTcgctgc                                                                           >  2:6478308/1‑150 (MQ=255)
                                                                         aGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTcgctgccgc                                                                        >  2:758046/1‑150 (MQ=255)
                                                                         aGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTcgctgccgc                                                                        >  1:5412916/1‑150 (MQ=255)
                                                                             cGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCgctgct                                                                    >  1:6673846/1‑150 (MQ=255)
                                                                             cGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCgctgct                                                                    >  2:9144007/1‑150 (MQ=255)
                                                                             cGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCgctgct                                                                    <  1:2732477/150‑1 (MQ=255)
                                                                                cTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGTCCGCGGCCCTGTCGCTGCCGCTGCTCgg                                                                 >  1:4210919/1‑150 (MQ=255)
                                                                                    gACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTg                                                             <  2:1198181/150‑1 (MQ=255)
                                                                                    gACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTg                                                             <  1:7008161/150‑1 (MQ=255)
                                                                                     aCCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTgg                                                            <  1:8969183/150‑1 (MQ=255)
                                                                                     aCCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTgg                                                            >  2:5217549/1‑150 (MQ=255)
                                                                                      ccGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCGGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTggc                                                           <  1:1554120/150‑1 (MQ=255)
                                                                                        gcgcCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTggcgg                                                         >  2:3693235/1‑150 (MQ=255)
                                                                                         cgcCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTggcggc                                                        >  2:9706076/1‑150 (MQ=255)
                                                                                           ccGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGcggcgg                                                      <  1:2134701/150‑1 (MQ=255)
                                                                                           ccGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGcggcgg                                                      >  1:1057110/1‑150 (MQ=255)
                                                                                           ccGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGcggcgg                                                      >  2:10358759/1‑150 (MQ=255)
                                                                                            cGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGt                                                     >  2:4656709/1‑150 (MQ=255)
                                                                                             gAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTg                                                    >  2:1020444/1‑150 (MQ=255)
                                                                                             gAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTg                                                    <  1:4625077/150‑1 (MQ=255)
                                                                                              aGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGa                                                   >  1:8654898/1‑150 (MQ=255)
                                                                                                gtgtCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAAt                                                 <  1:3751599/150‑1 (MQ=255)
                                                                                                gtgtCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAAt                                                 <  2:2458159/150‑1 (MQ=255)
                                                                                                 tgtCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATg                                                <  2:10697347/150‑1 (MQ=255)
                                                                                                   tCGGTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGcc                                              <  1:1725671/150‑1 (MQ=255)
                                                                                                     gTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGt                                            <  1:2235092/150‑1 (MQ=255)
                                                                                                      ttGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTc                                           <  1:8950370/150‑1 (MQ=255)
                                                                                                       tGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCt                                          <  1:5625498/150‑1 (MQ=255)
                                                                                                          tGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGc                                       <  2:2746064/150‑1 (MQ=255)
                                                                                                             tGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAAc                                    <  1:10726999/150‑1 (MQ=255)
                                                                                                                    gTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTCGCCACTCTAccc                             >  2:5972425/1‑148 (MQ=255)
                                                                                                                       cGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCt                          <  1:2883885/150‑1 (MQ=255)
                                                                                                                       cGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCt                          <  1:9042418/150‑1 (MQ=255)
                                                                                                                       cGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCCGGATGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCAGTCGGCGGTGAATGACGTCTGGCAACTCTAcgcgat                          >  1:10899961/1‑148 (MQ=255)
                                                                                                                        ggACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTg                         >  1:1933811/1‑150 (MQ=255)
                                                                                                                        ggACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTg                         <  2:80664/150‑1 (MQ=255)
                                                                                                                         gACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTGt                        >  1:5315438/1‑150 (MQ=255)
                                                                                                                         gACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTGt                        <  2:1306589/150‑1 (MQ=255)
                                                                                                                           cTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGTCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTGTTc                      <  2:8190919/150‑1 (MQ=255)
                                                                                                                           cTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTGTTc                      <  2:1487295/150‑1 (MQ=255)
                                                                                                                              gCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTGTTCctg                   <  1:577817/150‑1 (MQ=255)
                                                                                                                                ggTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTGTTCctgct                 <  2:7752083/150‑1 (MQ=255)
                                                                                                                                ggTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTGTTCctgct                 <  2:7118043/150‑1 (MQ=255)
                                                                                                                                ggTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTGTTCctgct                 <  1:8487671/150‑1 (MQ=255)
                                                                                                                                 gTCGCCATCACCAGCCCGCTCACGGGCTGGCTGCACGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCGACGCGCTGTTCctgctg                <  2:4487115/150‑1 (MQ=255)
                                                                                                                                 gTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTGTTCctgctg                <  1:6423339/150‑1 (MQ=255)
                                                                                                                                  tCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCt                          <  1:1456165/139‑1 (MQ=255)
                                                                                                                                  tCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTGTTCCTGCTGa               <  1:5669721/150‑1 (MQ=255)
                                                                                                                                  tCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTGTTCCTGCTGa               <  2:11004834/150‑1 (MQ=255)
                                                                                                                                   cGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTGTTCCTGCTGAt              <  1:251436/150‑1 (MQ=255)
                                                                                                                                       aTCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTGTTCCTGCTGATCGGc          >  1:6078743/1‑150 (MQ=255)
                                                                                                                                       aTCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTGTTCCTGCTGATCGGc          >  1:10134823/1‑150 (MQ=255)
                                                                                                                                       aTCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTGTTCCTGCTGATCGGc          >  1:4575367/1‑150 (MQ=255)
                                                                                                                                           ccAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTGTTCCTGCTGATCGGCctgc      >  1:5868574/1‑150 (MQ=255)
                                                                                                                                           ccAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTGTTCCTGCTGATCGGCctgc      >  2:8764421/1‑150 (MQ=255)
                                                                                                                                           ccAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTGTTCCTGCTGATCGGCctgc      <  2:1707190/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
GTGGCGATCATGCTCGGGCCCACCGGCATCCTGCTGAACACCTTCAGTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGTTCAAGGGCTGGCTGATCAACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTGTTCCTGCTGATCGGCCTGCTGGC  >  NC_008463/2651192‑2651484

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑