Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 791,862 3 bp→GCT coding (1842‑1844/5430 nt) pchF → pyochelin non‑ribosomal peptide synthetase PchF

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_008463791,8620CG99.6% 1892.8 / ‑2.1 500A614A (GCC→GCG) pchFpyochelin non‑ribosomal peptide synthetase PchF
Reads supporting (aligned to +/- strand):  ref base C (2/0);  new base G (321/176);  total (323/177)
Fisher's exact test for biased strand distribution p-value = 5.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01
*NC_008463791,8630TC99.6% 1916.3 / ‑3.3 500W615M (TGG→CTG) pchFpyochelin non‑ribosomal peptide synthetase PchF
Reads supporting (aligned to +/- strand):  ref base T (2/0);  new base C (321/177);  total (323/177)
Fisher's exact test for biased strand distribution p-value = 5.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*NC_008463791,8640GT99.4% 2046.2 / ‑5.9 500W615M (TGG→CTG) pchFpyochelin non‑ribosomal peptide synthetase PchF
Reads supporting (aligned to +/- strand):  ref base G (2/1);  new base T (321/175);  total (323/177)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 500 total aligned reads displayed.

CGCGGTGATGCTGCCGAAAAGCGCCGCGCAATTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCCCCGAAGTGGTCGCCTGGGTCTGCCTGGAAAGCGATGTCCCGAACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTGGCGGCGGACGACCTCGCCTACGTGATCTACACCTCCG  >  NC_008463/791721‑792004
                                                                                                                                             |||                                                                                                                                            
 gcgGTGATGCTGCCGAAAAGCGCCGCGCAATTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGc                                                                                                                                       >  1:9236802/1‑150 (MQ=255)
   ggTGATGCTGCCGAAAAGCGCCGCGCAATTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCt                                                                                                                                     >  1:6865818/1‑150 (MQ=255)
     tGATGCTGCCGAAAAGCGCCGCGCAATTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTgg                                                                                                                                   >  1:10626816/1‑150 (MQ=255)
       aTGCTGCCGAAAAGCGCCGCGCAATTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGaa                                                                                                                                 >  2:8303180/1‑150 (MQ=255)
       aTGCTGCCGAAAAGCGCCGCGCAATTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGaa                                                                                                                                 >  2:9372600/1‑150 (MQ=255)
       aTGCTGCCGAAAAGCGCCGCGCAATTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGaa                                                                                                                                 >  1:9505740/1‑150 (MQ=255)
           tgcCGAAAAGCGCCGCGCAATTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCGTGGAAAGCg                                                                                                                             >  2:10202434/1‑150 (MQ=255)
           tgcCGAAAAGCGCCGCGCAATTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCg                                                                                                                             >  1:2273611/1‑150 (MQ=255)
             ccGAAAAGCGCCGCGCAATTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGAt                                                                                                                           >  1:834038/1‑150 (MQ=255)
                aaaaGCGCCGCGCAATTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTc                                                                                                                        >  2:5006822/1‑150 (MQ=255)
                 aaaGCGCCGCGCAATTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTcc                                                                                                                       >  2:1591660/1‑150 (MQ=255)
                 aaaGCGCCGCGCAATTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTcc                                                                                                                       <  2:2311509/150‑1 (MQ=255)
                  aaGCGCCGCGCAATTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTccc                                                                                                                      >  2:5774593/1‑150 (MQ=255)
                  aaGCGCCGCGCAATTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTccc                                                                                                                      >  2:2624679/1‑150 (MQ=255)
                    gcgcCGCGCAATTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAACGCGATGTCCCgg                                                                                                                    >  2:5164297/1‑149 (MQ=255)
                     cgccgcGCAATTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCgga                                                                                                                   <  1:2108135/150‑3 (MQ=255)
                     cgccgcGCAATTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCgga                                                                                                                   <  1:2746902/150‑3 (MQ=255)
                         gcgcAATTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGcc                                                                                                               >  1:1581953/1‑150 (MQ=255)
                                    cgcgGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTg                                                                                                    >  1:9005478/1‑150 (MQ=255)
                                    cgcgGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTg                                                                                                    >  1:303979/1‑150 (MQ=255)
                                    cgcgGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTg                                                                                                    <  2:9934754/150‑1 (MQ=255)
                                    cgcgGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCGGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTg                                                                                                    >  2:3898042/1‑150 (MQ=255)
                                       ggTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGt                                                                                                 >  1:6999741/1‑150 (MQ=255)
                                           aTCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGcc                                                                                             <  1:5778240/150‑1 (MQ=255)
                                            tCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCa                                                                                            <  1:4508759/150‑1 (MQ=255)
                                            tCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCa                                                                                            >  2:9736944/1‑150 (MQ=255)
                                             cGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCAt                                                                                           <  1:5915084/150‑1 (MQ=255)
                                               gCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGCCATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCAGCg                                                                                         >  2:4624637/1‑150 (MQ=255)
                                               gCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGAGGTCGCGCTGGTCTGCCGGGAAAGCGAGGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCg                                                                                         >  2:2731544/1‑150 (MQ=255)
                                                 aTCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGAc                                                                                       >  1:10393917/1‑150 (MQ=255)
                                                  tcctccAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGCTCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCCTCGAcc                                                                                      >  1:10986917/1‑150 (MQ=255)
                                                    ctccAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCgg                                                                                    <  1:1222731/150‑1 (MQ=255)
                                                    ctccAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCgg                                                                                    >  1:10744077/1‑150 (MQ=255)
                                                    ctccAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCgg                                                                                    >  1:6655174/1‑150 (MQ=255)
                                                     tccAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGACGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGc                                                                                   <  2:9236199/150‑1 (MQ=255)
                                                      ccAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCt                                                                                  >  2:3719566/1‑150 (MQ=255)
                                                        aGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTgg                                                                                >  1:5252822/1‑150 (MQ=255)
                                                         ggCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGgc                                                                               >  1:984730/1‑150 (MQ=255)
                                                         ggCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGgc                                                                               >  1:186614/1‑150 (MQ=255)
                                                          gCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGgcc                                                                              >  1:975028/1‑150 (MQ=255)
                                                            cGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGgccgc                                                                            <  2:3463198/150‑1 (MQ=255)
                                                            cGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGgccgc                                                                            >  1:2335777/1‑150 (MQ=255)
                                                            cGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGgccgc                                                                            >  2:7217880/1‑150 (MQ=255)
                                                             ggCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGgccgcc                                                                           >  2:5554345/1‑150 (MQ=255)
                                                             ggCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGgccgcc                                                                           >  2:6956345/1‑150 (MQ=255)
                                                                 cAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACa                                                                       <  1:135527/150‑1 (MQ=255)
                                                                   gCCTCTGTGCCGGTGGACCTCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCCTCGACCGGCTGGCCGCCGACAgc                                                                     <  2:5871849/150‑1 (MQ=255)
                                                                   gCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAgc                                                                     >  2:8797634/1‑150 (MQ=255)
                                                                   gCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAgc                                                                     >  2:193151/1‑150 (MQ=255)
                                                                    ccTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAgcg                                                                    <  2:10488514/150‑1 (MQ=255)
                                                                       aTGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCt                                                                 >  1:8102113/1‑150 (MQ=255)
                                                                       aTGTGCCGGAGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCt                                                                 <  1:4904405/150‑1 (MQ=255)
                                                                          tgCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGgc                                                              <  2:3840434/150‑1 (MQ=255)
                                                                             cGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGgccgc                                                           <  2:1999023/150‑1 (MQ=255)
                                                                              ggTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGgccgcc                                                          >  1:8389492/1‑150 (MQ=255)
                                                                              ggTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGgccgcc                                                          >  1:5643968/1‑150 (MQ=255)
                                                                              ggTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCAGCGACCGGCTGGCCGCCGACTGCGCCTGgccgcc                                                          >  2:9850414/1‑150 (MQ=255)
                                                                               gTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCa                                                         >  2:6445382/1‑150 (MQ=255)
                                                                                 ggACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCAcc                                                       <  1:6369005/150‑1 (MQ=255)
                                                                                   aCATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCAccgc                                                     <  1:8725907/150‑1 (MQ=255)
                                                                                    cATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCAccgcc                                                    <  2:770609/150‑1 (MQ=255)
                                                                                      tCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCAccgcccg                                                  >  2:1282478/1‑150 (MQ=255)
                                                                                            aGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGcggcgg                                            >  2:10941157/1‑150 (MQ=255)
                                                                                            aGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGcggcgg                                            >  1:3965497/1‑150 (MQ=255)
                                                                                               cgccgcTGCGGCGCCAGGCGATCCTCGCCAGCGCCGACGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAgg                                         <  1:1030393/150‑1 (MQ=255)
                                                                                                gccgcTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGt                                        <  2:6747548/150‑1 (MQ=255)
                                                                                                gccgcTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGt                                        >  2:5649521/1‑150 (MQ=255)
                                                                                                 ccgcTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTg                                       >  2:6297332/1‑150 (MQ=255)
                                                                                                 ccgcTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGAGGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTg                                       >  2:961575/1‑150 (MQ=255)
                                                                                                  cgcTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTgg                                      <  1:10000376/150‑1 (MQ=255)
                                                                                                  cgcTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCGGGCCGCCACCGCCCGCGGCGGAGGTgg                                      <  2:6581310/150‑1 (MQ=255)
                                                                                                   gcTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCGGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTggc                                     <  2:6820734/150‑1 (MQ=255)
                                                                                                    cTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTggcg                                    <  2:2541795/150‑1 (MQ=255)
                                                                                                      gcggcgCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTggcggc                                  <  2:5412826/150‑1 (MQ=255)
                                                                                                       cggcgCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTGgcggcg                                 <  2:594674/150‑1 (MQ=255)
                                                                                                         gcgcCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTGGCGGCGga                               >  2:8509298/1‑150 (MQ=255)
                                                                                                            ccAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTGGCGGCGgacga                            >  2:2945352/1‑150 (MQ=255)
                                                                                                             cAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTGGCGGCGgacgac                           >  1:5575848/1‑150 (MQ=255)
                                                                                                               ggCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTGGCGGCGGACGACCt                         <  1:6390208/150‑1 (MQ=255)
                                                                                                               ggCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTGGCGGCGGACGACCt                         <  2:4652623/150‑1 (MQ=255)
                                                                                                                  gATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTGGCGGCGGACGACCTCGc                      >  1:7271438/1‑150 (MQ=255)
                                                                                                                   aTCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCCTCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTGGCGGCGGACGACCTCGcc                     >  2:7955997/1‑150 (MQ=255)
                                                                                                                   aTCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTGGCGGCGGACGACCTCGcc                     >  1:10495191/1‑150 (MQ=255)
                                                                                                                    tCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTGGCGGCGGACGACCTCGCCt                    >  1:3346940/1‑150 (MQ=255)
                                                                                                                    tCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTGGCGGCGGACGACCTCGCCt                    <  1:4116497/150‑1 (MQ=255)
                                                                                                                    tCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGATGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCATGGCCGCCACCGCCCGCGGCGGAGGTGGCGTCGGACGACCTCGcgt                    >  2:8160881/1‑148 (MQ=255)
                                                                                                                       acGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTGGCGGCGGACGACCTCGCCTACg                 <  1:9975546/149‑1 (MQ=255)
                                                                                                                        cGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTGGCGGCGGACGACCTCGCCTACGt                >  2:4856727/1‑150 (MQ=255)
                                                                                                                          ccAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTGGCGGCGGACGACCTCGCCTACGTGa              >  2:9056655/1‑150 (MQ=255)
                                                                                                                           cAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGTCGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGTGGTGGCGGCGGACGACCTCGCCTACGTGAt             >  1:355015/1‑150 (MQ=255)
                                                                                                                           cAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTGGCGGCGGACGACCTCGCCTACGTGAt             <  2:2824650/150‑1 (MQ=255)
                                                                                                                            aGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTGGCGGCGGACGACCTCGCCTACGTGATc            <  2:2430362/150‑1 (MQ=255)
                                                                                                                              cgccGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTGGCGGCGGACGACCTCGCCTACGTGATCTa          <  1:7701822/148‑1 (MQ=255)
                                                                                                                              cgccGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGAGGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTGGCGGCGGACGACCTCGCCTACGTGATCTa          <  2:4291501/148‑1 (MQ=255)
                                                                                                                               gccGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTGGCGGCGGACGACCTCGCCTACGTGATCTac         >  1:2400453/2‑150 (MQ=255)
                                                                                                                               gccGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCCACAGCGCCAGGCCGCCACCGGCCGCGGGGGGGGGGGCGGCGGACGACCTCGCCACCGTGAGCTac         >  2:6165774/2‑150 (MQ=255)
                                                                                                                                     gTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTGGCGGCGGACGACCTCGCCTACGTGATCTACACCTcc   <  1:3777046/150‑1 (MQ=255)
                                                                                                                                     gTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTGGCGGCGGACGACCTCGCCTACGTGATCTACACCTcc   >  2:3212361/1‑150 (MQ=255)
                                                                                                                                     gTGGTCGCGCTGGTCTGCCAGGAAAGCGATGTCCCGGCCGCCGGCTGCGCCTGCGTGGCCAACGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTGGCGGCGGACGACCTCGCCTACGTGATCTACACCTcc   <  1:5066922/150‑1 (MQ=255)
                                                                                                                                      tGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTGGCGGCGGACGACCTCGCCTACGTGATCTACACCTCCg  >  2:10730549/1‑150 (MQ=255)
                                                                                                                                             |||                                                                                                                                            
CGCGGTGATGCTGCCGAAAAGCGCCGCGCAATTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCCCCGAAGTGGTCGCCTGGGTCTGCCTGGAAAGCGATGTCCCGAACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTGGCGGCGGACGACCTCGCCTACGTGATCTACACCTCCG  >  NC_008463/791721‑792004

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑