BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_008463	3,670,537	Δ1 bp	coding (537/1020 nt)	PA14_RS16675 →	ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_008463	3,670,534	0	T	.	99.8%	2023.8 / ‑3.5	410	coding (534/1020 nt)	PA14_RS16675	ABC transporter permease
Reads supporting (aligned to +/- strand): ref base T (0/0); major base . (205/204); minor base A (1/0); total (206/204)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 423 total aligned reads displayed.

GCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCGTTTTCCTGG‑A‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCCGCGCCGAGTTCC  >  NC_008463/3670385‑3670679

gCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCGtt                                                                                                                                                    >  1:9299826/1‑151 (MQ=255)

     cgACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCGtttcctg                                                                                                                                               <  1:5895595/151‑5 (MQ=255)

      gACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTgg                                                                                                                                             >  1:3956947/1‑151 (MQ=255)

      gACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTgg                                                                                                                                             >  2:7643375/1‑151 (MQ=255)

       aCGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGG‑a                                                                                                                                           >  2:8559468/1‑151 (MQ=255)

          tGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGG‑aagt                                                                                                                                        <  1:11511903/151‑4 (MQ=255)

           gCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGG‑aagtc                                                                                                                                       <  1:3739698/151‑5 (MQ=255)

             tCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTg                                                                                                                                     <  1:470556/151‑1 (MQ=255)

             tCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCAACTACGGCGGCAGGGTAGACCTGCTCGGACATCG‑TTTCCTGGAA‑GTCTg                                                                                                                                     >  2:2581148/1‑151 (MQ=255)

                  cGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCgg                                                                                                                                >  2:8479660/1‑151 (MQ=255)

                  cGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCAGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCgg                                                                                                                                <  2:632168/151‑1 (MQ=255)

                    ggTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCgggg                                                                                                                              >  1:8804643/1‑151 (MQ=255)

                    ggTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCgggg                                                                                                                              <  2:4873734/151‑1 (MQ=255)

                      tGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCt                                                                                                                            <  1:1330980/151‑1 (MQ=255)

                        aTTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGc                                                                                                                          >  2:5859742/1‑151 (MQ=255)

                          ttACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCg                                                                                                                        >  1:3543172/1‑151 (MQ=255)

                            aCGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGt                                                                                                                      <  2:6087513/151‑1 (MQ=255)

                              ggCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGc                                                                                                                    <  1:3411907/151‑1 (MQ=255)

                               gCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGct                                                                                                                   >  1:1879650/1‑151 (MQ=255)

                                cTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGctc                                                                                                                  <  2:4348249/151‑1 (MQ=255)

                                 ttCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGctct                                                                                                                 <  1:10024074/151‑1 (MQ=255)

                                    cgcgTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTAcc                                                                                                              >  1:1497592/1‑151 (MQ=255)

                                     gcgTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACct                                                                                                             >  2:10728766/1‑151 (MQ=255)

                                     gcgTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACct                                                                                                             <  1:9575942/151‑1 (MQ=255)

                                      cgTGTCGATCCTCTTCGCCCAGACCCTGACCGTGCTCAGTTATCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGG‑ACGTCTGGGCGGGGCTGCCGGTGCTCTACctg                                                                                                            >  2:3932192/1‑151 (MQ=255)

                                        tgtCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACctgct                                                                                                          >  2:9743095/1‑151 (MQ=255)

                                        tgtCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACctgct                                                                                                          <  1:7337382/151‑1 (MQ=255)

                                          tCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGa                                                                                                        >  1:11394437/1‑151 (MQ=255)

                                           cGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGat                                                                                                       <  2:5420088/151‑1 (MQ=255)

                                             aTCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGatca                                                                                                     <  2:2069237/151‑1 (MQ=255)

                                              tCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGatcat                                                                                                    <  1:10158357/151‑1 (MQ=255)

                                                 tctTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCt                                                                                                 <  2:8721694/151‑1 (MQ=255)

                                                  ctTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTg                                                                                                >  1:6066747/1‑151 (MQ=255)

                                                  ctTAGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTg                                                                                                >  2:7428536/1‑151 (MQ=255)

                                                     cGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGcc                                                                                             >  1:3823063/1‑151 (MQ=255)

                                                     cGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGcc                                                                                             <  1:4640919/151‑1 (MQ=255)

                                                      gCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCGCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCa                                                                                            >  1:10280119/1‑151 (MQ=255)

                                                            accctgaccGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCCGCTTCg                                                                                      >  2:3265148/1‑151 (MQ=255)

                                                            accctgaccGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCg                                                                                      >  2:9464237/1‑151 (MQ=255)

                                                              cctgaccGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑ATTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTc                                                                                    >  2:5286219/1‑151 (MQ=255)

                                                               ctgaccGTGCTCAGTTCTCTTATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCTACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTcc                                                                                   >  2:2835711/1‑151 (MQ=255)

                                                               ctgaccGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTcc                                                                                   >  1:9130748/1‑151 (MQ=255)

                                                                 gaccGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAg                                                                                 <  2:6451144/151‑1 (MQ=255)

                                                                 gaccGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAg                                                                                 <  2:7110723/151‑1 (MQ=255)

                                                                   ccGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGcc                                                                               <  2:10917998/151‑1 (MQ=255)

                                                                    cGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCg                                                                              >  1:1975098/1‑151 (MQ=255)

                                                                    cGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCg                                                                              >  2:5490617/1‑151 (MQ=255)

                                                                      tGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGaa                                                                            >  1:858951/1‑151 (MQ=255)

                                                                      tGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGaa                                                                            >  2:10578089/1‑151 (MQ=255)

                                                                        cTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACt                                                                          >  2:2219835/1‑151 (MQ=255)

                                                                        cTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACt                                                                          <  1:3168010/151‑1 (MQ=255)

                                                                         tCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACtt                                                                         <  1:8046276/151‑1 (MQ=255)

                                                                          cAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTc                                                                        >  2:10166132/1‑151 (MQ=255)

                                                                           aGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCt                                                                       <  1:9452939/151‑1 (MQ=255)

                                                                            gTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCtg                                                                      >  2:10087658/1‑151 (MQ=255)

                                                                            gTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCtg                                                                      <  2:817733/151‑1 (MQ=255)

                                                                            gTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCtg                                                                      <  2:9020857/151‑1 (MQ=255)

                                                                                    aTCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGgctgc                                                              >  1:9875755/1‑151 (MQ=255)

                                                                                    aTCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑ATTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGgctgc                                                              >  2:6627866/1‑151 (MQ=255)

                                                                                     tCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGgctgct                                                             <  1:4551813/151‑1 (MQ=255)

                                                                                      cGGCGTCACCGTCGGCGCCCTGCAAGGCTACTCCGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGctgctg                                                            <  2:1263836/151‑1 (MQ=255)

                                                                                         cGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGc                                                         <  2:2294693/151‑1 (MQ=255)

                                                                                         cGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGc                                                         <  2:835044/151‑1 (MQ=255)

                                                                                          gTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTCCCTGCTGAACAACCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTgggaa                                                        >  1:2333520/1‑149 (MQ=255)

                                                                                           tCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGcat                                                       >  1:6404402/1‑151 (MQ=255)

                                                                                            cACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGcatc                                                      <  1:4872086/151‑1 (MQ=255)

                                                                                             aCCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGcatca                                                     >  2:11317456/1‑151 (MQ=255)

                                                                                               cGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCAtg                                                   <  1:8341709/151‑1 (MQ=255)

                                                                                                 tCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCAtgct                                                 <  2:4734393/151‑1 (MQ=255)

                                                                                                  cGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCAtgctg                                                <  1:1171439/151‑1 (MQ=255)

                                                                                                   ggCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCAtgctgc                                               <  2:7487697/151‑1 (MQ=255)

                                                                                                      gcCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGt                                            >  2:2740077/1‑151 (MQ=255)

                                                                                                      gcCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGt                                            <  2:11394437/151‑1 (MQ=255)

                                                                                                        ccTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGttt                                          >  2:9862744/1‑151 (MQ=255)

                                                                                                        ccTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGttt                                          <  2:453839/151‑1 (MQ=255)

                                                                                                          tGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTc                                        <  1:10456548/151‑1 (MQ=255)

                                                                                                             aaGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTg                                     <  2:1099939/151‑1 (MQ=255)

                                                                                                               ggCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGa                                   <  1:10977165/151‑1 (MQ=255)

                                                                                                                gCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGAt                                  <  1:10585334/151‑1 (MQ=255)

                                                                                                                  tactacGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGa                                >  2:5447658/1‑151 (MQ=255)

                                                                                                                    ctacGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGc                              <  1:2718853/151‑1 (MQ=255)

                                                                                                                     tacGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGcc                             <  1:1789309/151‑1 (MQ=255)

                                                                                                                      acGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCt                            >  1:7804060/1‑151 (MQ=255)

                                                                                                                        ggcggcTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCtgg                          >  2:9384343/1‑151 (MQ=255)

                                                                                                                          cggcTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCtggtg                        <  2:3587484/151‑1 (MQ=255)

                                                                                                                           ggcTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCtggtgg                       >  1:5514718/1‑151 (MQ=255)

                                                                                                                            gcTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGa                      >  1:699590/1‑151 (MQ=255)

                                                                                                                             cTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGAc                     <  1:6700135/151‑1 (MQ=255)

                                                                                                                              tGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACg                    >  2:8045134/1‑151 (MQ=255)

                                                                                                                               gggTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGt                   <  2:5115890/151‑1 (MQ=255)

                                                                                                                                ggTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTg                  <  2:6828794/151‑1 (MQ=255)

                                                                                                                                ggTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTg                  <  1:5133083/151‑1 (MQ=255)

                                                                                                                                   cGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTc               >  1:8920879/1‑151 (MQ=255)

                                                                                                                                    gACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTcc              >  2:7157269/1‑151 (MQ=255)

                                                                                                                                     aCCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCcg             <  1:9045726/151‑1 (MQ=255)

                                                                                                                                              gCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCCGCGCCGAGtt    >  2:1515207/1‑151 (MQ=255)

                                                                                                                                              gCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCCGCGCCGAGtt    >  1:7697790/1‑151 (MQ=255)

                                                                                                                                               ccAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCCGCGCCGAGTtc   >  1:4158258/1‑151 (MQ=255)

                                                                                                                                                cAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCCGCGCCGAGTtcc  >  1:7545051/1‑151 (MQ=255)

                                                                                                                                                cAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCCGCGCCGAGTtcc  >  2:1968340/1‑151 (MQ=255)

GCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCGTTTTCCTGG‑A‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCCGCGCCGAGTTCC  >  NC_008463/3670385‑3670679

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`