Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 5,028,119 Δ1 bp intergenic (‑41/+96) PA14_RS22975 ← / ← PA14_RS22980 Nramp family divalent metal transporter/ribbon‑helix‑helix domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084635,028,1170C.99.6% 2522.2 / 3.1 516intergenic (‑39/+98)PA14_RS22975/PA14_RS22980Nramp family divalent metal transporter/ribbon‑helix‑helix domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base C (2/0);  new base . (218/293);  total (222/294)
Fisher's exact test for biased strand distribution p-value = 1.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01

Only 100 of 537 total aligned reads displayed.

CGATGGCGGTGGCCCAGTTGCCGGGGTCCATGTAGCCGACCGAGACCAGCAGGCCGGGGCCGACGAACAGCGCCAGCTTGCGCAGCGGCGAGGCGTCGGCCGGCACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGGCCCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCCGGGTGGGTTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGC  >  NC_008463/5027967‑5028264
                                                                                                                                                      |                                                                                                                                                   
  agggCGGTGGCCCAGTTGCCGGGGTCCATGTAGCCGACCGAGACCAGCAGGCCGGGGCCGACGAACAGCGCCAGCTTGCGCAGCGGCGAGGCGTCGGCCGGCACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGGccg                                                                                                                                                   <  2:8335297/149‑2 (MQ=255)
    ggCGGTGGCCCAGTTGCCGGGGTCCATGTAGCCGACCGAGACCAGCAGGCCGGGGCCGACGAACAGCGCCAGCTTGCGCAGCGGCGAGGCGTCGGCCGGCACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGGccgtg                                                                                                                                                 <  1:6360486/151‑4 (MQ=255)
      cGGTGGCCCAGTTGCCGGGGTCCATGTAGCCGACCGAGACCAGCAGGCCGGGGCCGACGAACAGCGCCAGCTTGCGCAGCGGCGAGGCGTCGGCCGGCACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGGccggggt                                                                                                                                               >  1:1401211/1‑146 (MQ=255)
        gTGGCCCAGTTGCCGGGGTCCATGTAGCCGACCGAGACCGGCAGGCCGGGGCCGACGAACAGCGCCAGCTTGCGCAGCGGCGAGGCGTCGGCCGGCACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGtggtgg                                                                                                                                            >  2:4711320/1‑151 (MQ=255)
         tGGCCCAGTTGCCGGGGTCCATGTAGCCGACCGAGACCAGCAGGCCGGGGCCGACGAACAGCGCCAGCTTGCGCAGCGGCGAGGCGTCGGCCGGCACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTggg                                                                                                                                           <  2:11387170/151‑1 (MQ=255)
          ggCCCAGTTGCCGGGGTCCATGTAGCCGACCGAGACCAGCAGGCCGGGGCCGACGAACAGCGCCAGCTTGCGCAGCGGCGAGGCGTCGGCCGGCACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGc                                                                                                                                          <  2:1751490/151‑1 (MQ=255)
           gCCCAGTTGCCGGGGTCCATGTAGCCGACCGAGACCCGCAGGCCGGGGCCGACGAACAGCGCCAGCTTGCGCAGCGGCGAGGCGTCGGCCGGCACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCa                                                                                                                                         <  2:7964304/151‑1 (MQ=255)
              cAGTTGCCGGGGTCCATGTAGCCGACCGAGACCAGCAGGCCGGGGCCGACGAACAGCGCCCGCTTGCGCAGCGGCGAGGCGTCGGCCGGCACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAggg                                                                                                                                      <  2:5662118/151‑1 (MQ=255)
              cAGTTGCCGGGGTCCATGTAGCCGACCGAGACCAGCAGGCCGGGGCCGACGAACAGCGCCAGCTTGCGCAGCGGCGAGGCGTCGGCCGGCACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAggg                                                                                                                                      <  1:4080135/151‑1 (MQ=255)
                 ttGCCGGGGTCCATGTAGCCGACCGAGACCAGCAGGCCGGGGCCGACGAACAGCGCCAGCTTGCGCAGCGGCGAGGCGTCGGCCGGCACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGt                                                                                                                                   <  2:11621781/151‑1 (MQ=255)
                  tGCCGGTGTCCATGTAGCCGACCGATACCAGCAGTCCGGTGCCGACGAACAGCGCCAGCTTGCGCAGCTGCGAGGCGTCGGCCGGCACCGCCACGCTGTGGATGACCGCCGAGGGTCAGAAAGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTa                                                                                                                                  <  2:10398897/151‑1 (MQ=255)
                  tGCCGGGGTCCATGTAGCCGACCGAGACCAGCAGGCCGGGGCCGACGAACAGCGCCAGCTTGCGCAGCGGCGAGGCGTCGGCCGGCACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTa                                                                                                                                  >  2:1360606/1‑151 (MQ=255)
                  tGCCGGGGTCCATGTAGCCGACCGAGACCAGCAGGCCGGGGCCGACGAACAGCGCCAGCTTGCGCAGCGGCGAGGCGTCGGCCGGCACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGGGGGCAGGGAGTa                                                                                                                                  >  2:556660/1‑151 (MQ=255)
                   gCCGGGGTCCATGTAGCCGACCGAGACCAGCAGGCCGGGGCCGACGAACAGCGCCAGCTTGCGCAGCGGCGAGGCGTCGGCCGGCACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTAt                                                                                                                                 <  1:10555658/151‑1 (MQ=255)
                     cGGGGTCCATGTAGCCGACCGAGACCAGCAGGCCGGGGCCGACGAACAGCGCCAGCTTGCGCAGCGGCGAGGCGTCGGCCGGCACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTAttt                                                                                                                               <  1:5290966/151‑1 (MQ=255)
                      ggggTCCATGTAGCCGACCGAGACCAGCAGGCCGGGGCCGACGCACAGCGCCAGCTTGCGCAGCGGCGAGGCGTCGGCCGGCACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTc                                                                                                                              <  2:3860270/151‑1 (MQ=255)
                      ggggTCCATGTAGCCGACCGAGACCAGCAGGCCGGGGCCGACGAACAGCGCCAGCTTGCGCAGCGGCGAGGCGTCGGCCGGCACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTc                                                                                                                              >  1:6921821/1‑151 (MQ=255)
                       gggTCCATGTAGCCGACCGAGACCAGCAGGCCGGGGCCGACGAACAGCGCCAGCTTGCGCAGCGGCGAGGCGTCGGCCGGCACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCa                                                                                                                             <  1:9302151/151‑1 (MQ=255)
                       gggTCCATGTAGCCGACCGAGACCAGCAGGCCGGGGCCGACGAACAGCGCCAGCTTGCGCAGCGGCGAGGCGTCGGCCGGCACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCa                                                                                                                             <  1:10385305/151‑1 (MQ=255)
                           ccATGTAGCCGACCGAGACCAGCAGGCCGGGGCCGACGAACAGCGCCAGCTTGCGCAGCGGCGAGGCGTCGGCCGGCACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAg                                                                                                                         <  2:2745330/151‑1 (MQ=255)
                            cATGTAGCCGACCGAGACCAGCAGGCCGGGGCCGACGAACAGCGCCAGCTTGCGCAGCGGCGAGGCGTCGGCCGGCACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAgg                                                                                                                        >  2:2534054/1‑151 (MQ=255)
                            cATGTAGCCGACCGAGACCAGCAGGCCGGGGCCGACGAACAGCGCCAGCTTGCGCAGCGGCGAGGCGTCGGCCGGCACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAgg                                                                                                                        <  1:3046969/151‑1 (MQ=255)
                            cATGTAGCCGACCGAGACCAGCAGGCCGGGGCCGACGAACAGCGCCAGCTTGCGCAGCGGCGAGGCGTCGGCCGGCACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAgg                                                                                                                        >  1:2114266/1‑151 (MQ=255)
                               gTAGCCGACCGAGACCAGCAGGCCGGGGCCGACGAACAGCGCCAGCTTGCGCAGCGGCGAGGCGTCGGCCGGCACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGa                                                                                                                     <  2:7832949/151‑1 (MQ=255)
                               gTAGCCGACCGAGACCAGCAGGCCGGGGCCGACGAACAGCGCCAGCTTGCGCAGCGGCGAGGCGTCGGCCGGCACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGa                                                                                                                     >  2:5304507/1‑151 (MQ=255)
                                 aGCCGACCGAGACCAGCAGGCCGGGGCCGACGAACAGCGCCAGCTTGCGCAGCGGCGAGGCGTCGGCCGGCACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACg                                                                                                                   <  2:4372314/151‑1 (MQ=255)
                                     gaccgaGACCAGCAGGCCGGGGCCGACGAACAGCGCCAGCTTGCGCAGCGGCGAGGCGTCGGCCGGCACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCgg                                                                                                               <  2:6247413/151‑1 (MQ=255)
                                     gaccgaGACCAGCAGGCCGGGGCCGACGAACAGCGCCAGCTTGCGCAGCGGCGAGGCGTCGGCCGGCACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCgg                                                                                                               <  2:10822493/151‑1 (MQ=255)
                                        cgaGACCAGCAGGCCGGGGCCGACGAACAGCGCCAGCTTGCGCAGCGGCGAGGCGTCGGCCGGCACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATc                                                                                                            <  2:11052647/151‑1 (MQ=255)
                                          agaCCAGCAGGCCGGGGCCGACGAACAGCGCCAGCTTGCGCAGCGGCGAGGCGTCGGCCGGCACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCgg                                                                                                          <  2:10028318/151‑1 (MQ=255)
                                           gaCCAGCAGGCCGGGGCCGACGAACAGCGCCAGCTTGCGCAGCGGCGAGGCGTCGGCCGGCACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCggg                                                                                                         <  2:1930614/151‑1 (MQ=255)
                                             ccAGCAGGCCGGGGCCGACGAACAGCGCCAGCTTGCGCAGCGGCGAGGCGTCGGCCGGCACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGgga                                                                                                       <  1:6258675/151‑1 (MQ=255)
                                             ccAGCAGGCCGGGGCCGACGAACAGCGCCAGCTTGCGCAGCGGCGAGGCGTCGGCCGGCACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGgga                                                                                                       <  2:2006774/151‑1 (MQ=255)
                                                  agGCCGGGGCCGACGAACAGCGCCAGCTTGCGCAGCGGCGAGGCGTCGGCCGGCACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAg                                                                                                  <  1:2384832/151‑1 (MQ=255)
                                                  agGCCGGGGCCGACGAACAGCGCCAGCTTGCGCAGCGGCGAGGCGTCGGCCGGCACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATGTCTCGAGCCGACGCCGGTTCGGGGAGGAAg                                                                                                  >  1:5668833/1‑151 (MQ=255)
                                                     ccGGGGCCGACGAACAGCGCCAGCTTGCGCAGCGGCGAGGCGTCGGCCGGCACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGc                                                                                               <  2:9675054/151‑1 (MQ=255)
                                                     ccGGGGCCGACGAACAGCGCCAGCTTGCGCAGCGGCGAGGCGTCGGCCGGCACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGc                                                                                               >  1:8588928/1‑151 (MQ=255)
                                                      cGGGGCCGACGAACAGCGCCAGCTTGCGCAGCGGCGAGGCGTCGGCCGGCACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCg                                                                                              <  1:3543059/151‑1 (MQ=255)
                                                       ggggCCGACGAACAGCGCCAGCTTGCGCAGCGGCGAGGCGTCGGCCGGCACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑TCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGa                                                                                             >  2:8612845/1‑151 (MQ=255)
                                                         ggCCGACGAACAGCGCCAGCTTGCGCAGCGGCGAGGCGTCGGCCGGCACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGAcc                                                                                           >  2:5758910/1‑151 (MQ=255)
                                                         ggCCGACGAACAGCGCCAGCTTGCGCAGCGGCGAGGCGTCGGCCGGCACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGAcc                                                                                           >  1:8351038/1‑151 (MQ=255)
                                                          gCCGACGAACAGCGCCAGCTTGCGCAGCGGCGAGGCGTCGGCCGGCACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCa                                                                                          <  1:9629775/151‑1 (MQ=255)
                                                          gCCGACGAACAGCGCCAGCTTGCGCAGCGGCGAGGCGTCGGCCGGCACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCa                                                                                          <  1:4797425/151‑1 (MQ=255)
                                                          gCCGACGAACAGCGCCAGCTTGCGCAGCGGCGAGGCGTCGGCCGGCACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCa                                                                                          >  2:6071794/1‑151 (MQ=255)
                                                          gCCGACGAACAGCGCCAGCGTGCGCAGCGGCGAGGCGTCGGCCGGCACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCa                                                                                          <  1:2300383/151‑1 (MQ=255)
                                                            cgacgaACAGCGCCAGCTTGCGCAGCGGCGAGGCGTCGGCCGGCACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGc                                                                                        >  2:988039/1‑151 (MQ=255)
                                                            cgacgaACAGCGCCAGCTTGCGCAGCGGCGAGGCGTCGGCCGGCACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGc                                                                                        >  2:9810858/1‑151 (MQ=255)
                                                            cgacgaACAGCGCCAGCTTGCGCAGCGGCGAGGCGTCGGCCGGCACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGc                                                                                        >  2:5438194/1‑151 (MQ=255)
                                                            cgacgaACAGCGCCAGCTTGCGCAGCGGCGAGGCGTCGGCCGGCACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGc                                                                                        >  1:7540643/1‑151 (MQ=255)
                                                             gacgaACAGCGCCAGCTTGCGCAGCGGCGAGGCGTCGGCCGGCACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTGTTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGcc                                                                                       >  1:1114383/1‑151 (MQ=255)
                                                             gacgaACAGCGCCAGCTTGCGCAGCGGCGAGGCGTCGGCCGGCACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGca                                                                                       >  2:11173679/1‑150 (MQ=255)
                                                              acgaACAGCGCCAGCTTGCGCAGCGGCGAGGCGTCGGCCGGCACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCt                                                                                      <  1:11406713/151‑1 (MQ=255)
                                                               cgaACAGCGCCAGCTTGCGCAGCGGCGAGGCGTCGGCCGGCACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTa                                                                                     <  2:6532029/151‑1 (MQ=255)
                                                                 aaCAGCGCCAGCTTGCGCAGCGGCGAGGCGTCGGCCGGCACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTaaa                                                                                   >  2:8993479/1‑151 (MQ=255)
                                                                    aGCGCCAGCTTGCGCAGCGGCGAGGCGTCGGCCGGCACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGt                                                                                <  1:3777293/151‑1 (MQ=255)
                                                                    aGCGCCAGCTTGCGCAGCGGCGAGGCGTCGGCCGGCACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGt                                                                                >  2:9495936/1‑151 (MQ=255)
                                                                     gcgcCAGCTTGCGCAGCGGCGAGGCGTCGGCCGGCACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGtt                                                                               <  1:7070700/151‑1 (MQ=255)
                                                                     gcgcCAGCTTGCGCAGCGGCGAGGCGTCGGCCGGCACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGT‑GTCCGTGTTGGGCAGGGAGTATTTCACGTGGCGACGCCGGATCGGGGAGGAAGTGCGACCTGCCTAAACGtt                                                                               <  2:8057840/151‑1 (MQ=255)
                                                                      cgcCAGCTTGCGCAGCGGCGAGGCGTCGGCCGGCACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGttt                                                                              >  1:5056565/1‑151 (MQ=255)
                                                                       gcCAGCTTGCGCCGCGGCGAGGCGTCGGCCGGCACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATGTCACGAGGCGACGCCGGTTCGGGGAGGAAGTGCGACCAGCCTCTACGTTTc                                                                             >  2:777343/1‑151 (MQ=255)
                                                                         cAGCTTGCGCAGCGGCGAGGCGTCGGCCGGCACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCAc                                                                           <  2:7541349/151‑1 (MQ=255)
                                                                          aGCTTGCGCAGCGGCGAGGCGTCGGCCGGCACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCAcc                                                                          <  1:7102048/151‑1 (MQ=255)
                                                                           gCTTGCGCAGCGGCGAGGCGTCGGCCGGCACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCAccc                                                                         <  2:3521375/151‑1 (MQ=255)
                                                                             ttGCGCAGCGGCGAGGCGTCGGCCGGCACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCCg                                                                       >  2:8771050/1‑151 (MQ=255)
                                                                                 gcAGCGGCGAGGCGTCGGCCGGCACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCCggtcg                                                                   >  2:4074247/1‑148 (MQ=255)
                                                                                 gcAGCGGCGAGGCGTCGGCCGGCACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCCggtcg                                                                   <  1:10879544/151‑4 (MQ=255)
                                                                                   aGCGGCGAGGCGTCGGCCGGCACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAATCGTTTCACCCCggtcgcg                                                                 >  2:10625573/1‑146 (MQ=255)
                                                                                   aGCGGCGAGGCGTCGGCCGGCACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCCggtcgcg                                                                 <  1:908792/151‑6 (MQ=255)
                                                                                     cggcgAGGCGTCGGCCGGCACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCCggtcgcgtc                                                               >  1:6746193/1‑144 (MQ=255)
                                                                                            gCGTCGGCCGGCACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTtccctc                                                       <  2:6823561/151‑1 (MQ=255)
                                                                                             cGTCGGCCGGCACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTtccctcc                                                      >  2:6189047/1‑151 (MQ=255)
                                                                                             cGTCGGCCGGCACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTtccctcc                                                      <  1:9041562/151‑1 (MQ=255)
                                                                                              gTCGGCCGGCACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTtccctccc                                                     >  1:10725891/1‑151 (MQ=255)
                                                                                               tCGGCCGGCACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTccctcccc                                                    >  2:4650680/1‑151 (MQ=255)
                                                                                                  gccggcACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCa                                                 <  2:10934199/151‑1 (MQ=255)
                                                                                                  gccggcACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCa                                                 <  2:4931116/151‑1 (MQ=255)
                                                                                                  gccggcACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCa                                                 <  2:2208089/151‑1 (MQ=255)
                                                                                                   ccggcACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAg                                                >  1:829733/1‑151 (MQ=255)
                                                                                                    cggcACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAgg                                               >  2:7148708/1‑151 (MQ=255)
                                                                                                       cACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCt                                            >  1:7857549/1‑151 (MQ=255)
                                                                                                          cGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTggg                                         >  1:2556021/1‑151 (MQ=255)
                                                                                                              aCGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGgcgcg                                     <  2:3154615/151‑1 (MQ=255)
                                                                                                               cGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGgcgcgc                                    >  2:6673751/1‑151 (MQ=255)
                                                                                                                 cTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGGGGGCACGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGACGTGCGACCAGCCGAAACGTTTCACCCC‑GGTCGCGTCTACCCTCCCCTCAGGCCTGGGCGCTCTg                                  >  1:4442187/1‑151 (MQ=255)
                                                                                                                   gtgGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGc                                <  2:8823999/151‑1 (MQ=255)
                                                                                                                    tgGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGAGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGcc                               <  1:7942861/151‑1 (MQ=255)
                                                                                                                        tGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGAcc                           >  2:8746508/1‑151 (MQ=255)
                                                                                                                          aCCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGCCGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAg                         <  1:166447/151‑1 (MQ=255)
                                                                                                                          aCCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAg                         <  1:178689/151‑1 (MQ=255)
                                                                                                                          aCCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAg                         <  1:10041194/151‑1 (MQ=255)
                                                                                                                                gAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAg                   >  1:3376254/1‑151 (MQ=255)
                                                                                                                                  gggACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCt                 >  1:5383814/1‑151 (MQ=255)
                                                                                                                                   ggACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTc                >  2:4416069/1‑151 (MQ=255)
                                                                                                                                   ggACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTc                >  1:8665896/1‑151 (MQ=255)
                                                                                                                                    gACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCg               <  1:9784409/151‑1 (MQ=255)
                                                                                                                                     aCAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCCCGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCgg              <  2:10519226/151‑1 (MQ=255)
                                                                                                                                              cgcgGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTCAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTtct     >  2:9238426/1‑151 (MQ=255)
                                                                                                                                               gcgGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTtctc    >  2:7339033/1‑151 (MQ=255)
                                                                                                                                               gcgGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTtctc    >  1:10256449/1‑151 (MQ=255)
                                                                                                                                                cgGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTcg   <  1:6083855/151‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                   
CGATGGCGGTGGCCCAGTTGCCGGGGTCCATGTAGCCGACCGAGACCAGCAGGCCGGGGCCGACGAACAGCGCCAGCTTGCGCAGCGGCGAGGCGTCGGCCGGCACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGGCCCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCCGGGTGGGTTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGC  >  NC_008463/5027967‑5028264

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑