BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_008463	5,358,513	Δ1 bp	pseudogene (774/1285 nt)	PA14_RS24540 →	site‑specific integrase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_008463	5,358,510	0	T	.	99.6%	531.7 / ‑3.4	269	pseudogene (771/1285 nt)	PA14_RS24540	site‑specific integrase
Reads supporting (aligned to +/- strand): ref base T (0/0); major base . (123/145); minor base C (1/0); total (124/145)
Fisher's exact test for biased strand distribution p-value = 4.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 285 total aligned reads displayed.

CGATAGAGCCGGATTCGCCGATGACGACCAGTTGTTCAACGTCAACCGGTTCTCACCGCACTACAAGAGCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCGTTTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACTACGATCACATGCGTGCCGTGCTGCATG  >  NC_008463/5358360‑5358656

cGATAGAGCCGGATTCGCCGATGACGACCAGTTGTTCAACGTCAACCGGTTCTCACCGCACTACAAGAGCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCGt                                                                                                                                                    >  2:3889257/1‑151 (MQ=31)

cGATAGAGCCGGATTCGCCGATGACGACCAGTTGTTCAACGTCAACCGGTTCTCACCGCACTACAAGAGCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCGt                                                                                                                                                    >  2:11392845/1‑151 (MQ=31)

 gATAGAGCCGGATTCGCCGATGACGACCAGTTGTTCAACGTCAACCGGTTCTCACCGCACTACAAGAGCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCGtt                                                                                                                                                   >  2:2659805/1‑151 (MQ=31)

  aTAGAGCCGGATTCGCCGATGACGACCAGTTGTTCAACGTCAACCGGTTCTCACCGCACTACAAGAGCAAGGTGATGAACTCCGACCTGGTCGAAGCCATGTACCGGAAGTTGAACGAGAAGGTTGGGGTGCGGATGACCCCGCACCGttt                                                                                                                                                  <  2:750666/151‑1 (MQ=14)

  aTAGAGCCGGATTCGCCGATGACGACCAGTTGTTCAACGTCAACCGGTTCTCACCGCACTACAAGAGCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCGttt                                                                                                                                                  >  2:8276378/1‑151 (MQ=31)

    agagCCGGATTCGCCGATGACGACCAGTTGTTCAACGTCAACCGGTTCTCACCGCACTACAAGAGCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCGtttcc                                                                                                                                                <  2:11620588/151‑3 (MQ=11)

    agagCCGGATTCGCCGATGACGACCAGTTGTTCAACGTCAACCGGTTCTCACCGCACTACAAGAGCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCGtttcc                                                                                                                                                >  1:4355825/1‑149 (MQ=11)

     gagCCGGATTCGCCGATGACGACCAGTTGTTCAACGTCAACCGGTTCTCACCGCACTACAAGAGCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCGtttccg                                                                                                                                               <  1:1743821/151‑4 (MQ=1)

                                                    tcACCGCACTACAAGAGCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTc                                                                                               >  1:5118809/1‑151 (MQ=1)

                                                     cACCGCACTACAAGAGCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCa                                                                                              <  2:3850995/151‑1 (MQ=11)

                                                     cACCGCACTACAAGAGCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCa                                                                                              >  2:11045821/1‑151 (MQ=11)

                                                     cACCGCACTACAAGAGCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCa                                                                                              >  1:892351/1‑151 (MQ=11)

                                                     cACCGCACTACAAGAGCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCa                                                                                              <  1:8633944/151‑1 (MQ=11)

                                                      aCCGCACTACAAGAGCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCAc                                                                                             >  2:8632628/1‑151 (MQ=11)

                                                        cGCACTACAAGAGCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCt                                                                                           <  2:5777541/151‑1 (MQ=11)

                                                         gCACTACACGAGCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTc                                                                                          <  1:850626/151‑1 (MQ=2)

                                                            cTACAAGAGCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACg                                                                                       <  1:519758/151‑1 (MQ=11)

                                                            cTACAAGAGCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACg                                                                                       <  1:7318661/151‑1 (MQ=11)

                                                            cTACAAGAGCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACg                                                                                       >  1:10092114/1‑151 (MQ=11)

                                                             tACAAGAGCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGa                                                                                      >  2:5114106/1‑151 (MQ=11)

                                                              aCAAGAGCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGaa                                                                                     >  2:2131683/1‑151 (MQ=11)

                                                              aCAAGAGCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGaa                                                                                     <  2:9385984/151‑1 (MQ=11)

                                                               cAAGAGCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAg                                                                                    <  2:8041929/151‑1 (MQ=11)

                                                                aaGAGCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGATGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCTCCCGAGCGGAACATTCACCTCACGAAGt                                                                                   <  1:339675/151‑1 (MQ=2)

                                                                aaGAGCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGt                                                                                   <  1:8324766/151‑1 (MQ=11)

                                                                  gagCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGc                                                                                 <  2:9233539/151‑1 (MQ=11)

                                                                  gagCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTGGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGCCTTGATGAAGGCACCCGAGCGGAACAGTCACCTCACGAAGTGc                                                                                 >  2:8739923/1‑151 (MQ=2)

                                                                    gCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCt                                                                               <  2:4238005/151‑1 (MQ=11)

                                                                    gCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCt                                                                               <  2:492657/151‑1 (MQ=11)

                                                                    gCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCt                                                                               >  2:7294515/1‑151 (MQ=11)

                                                                    gCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCt                                                                               <  1:11132561/151‑1 (MQ=11)

                                                                     cAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTg                                                                              >  1:955384/1‑151 (MQ=11)

                                                                     cAAGGTGATGAACTCCGACCAGGTCGAAGCCATGGACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGATCATTCACCTCACGAAGTGCCTg                                                                              <  2:1511581/151‑1 (MQ=2)

                                                                     cAAGGTGATGAACCCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTg                                                                              >  2:11061897/1‑151 (MQ=2)

                                                                       aGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCt                                                                            >  2:5232346/1‑151 (MQ=11)

                                                                       aGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCt                                                                            <  1:8135370/151‑1 (MQ=11)

                                                                           gatgaACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAAc                                                                        <  2:4837715/151‑1 (MQ=11)

                                                                           gatgaACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAAc                                                                        >  1:6877909/1‑151 (MQ=11)

                                                                            atgaACTCCTACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAAcc                                                                       <  2:4098504/151‑1 (MQ=2)

                                                                            atgaACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAAcc                                                                       <  1:10413059/151‑1 (MQ=11)

                                                                            atgaACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAAcc                                                                       <  1:3778461/151‑1 (MQ=11)

                                                                                aCTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTc                                                                   <  1:1862519/151‑1 (MQ=11)

                                                                                aCTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTc                                                                   <  2:3115961/151‑1 (MQ=11)

                                                                                aCTCCGACCACGTCGACGCCAAGAACCGGCAGTTGACCCCGCAGGTTGGGGGGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTc                                                                   <  2:7152959/151‑1 (MQ=11)

                                                                                 cTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCg                                                                  <  2:3282373/151‑1 (MQ=11)

                                                                                 cTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCg                                                                  <  2:10849535/151‑1 (MQ=11)

                                                                                  tCCGACCAGGTCGAAGCCATGGACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGa                                                                 <  2:2805227/151‑1 (MQ=2)

                                                                                      aCCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAatat                                                             <  2:2563153/151‑1 (MQ=11)

                                                                                      aCCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAatat                                                             <  1:11323733/151‑1 (MQ=11)

                                                                                       ccAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATc                                                            >  1:2832176/1‑151 (MQ=11)

                                                                                        cAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATcc                                                           <  1:3887912/151‑1 (MQ=11)

                                                                                         aGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCa                                                          <  1:6097586/151‑1 (MQ=11)

                                                                                            tCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGAc                                                       >  1:10366341/1‑151 (MQ=11)

                                                                                             cGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGAcc                                                      >  1:1903925/1‑151 (MQ=11)

                                                                                             cGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGAcc                                                      <  1:2420699/151‑1 (MQ=11)

                                                                                             cGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGAcc                                                      >  1:776276/1‑151 (MQ=11)

                                                                                              gAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCa                                                     >  2:6698962/1‑151 (MQ=11)

                                                                                                aGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACg                                                   >  1:7430479/1‑151 (MQ=14)

                                                                                                aGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACg                                                   <  2:7202792/151‑1 (MQ=14)

                                                                                                      gTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGc                                             >  2:6496392/1‑151 (MQ=17)

                                                                                                       tACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCt                                            >  1:6806683/1‑151 (MQ=17)

                                                                                                       tACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCt                                            >  2:2179450/1‑151 (MQ=17)

                                                                                                        aCCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTa                                           >  1:5683515/1‑151 (MQ=17)

                                                                                                         ccGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTAc                                          <  1:7294094/151‑1 (MQ=17)

                                                                                                          cGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACa                                         >  1:3437091/1‑151 (MQ=17)

                                                                                                           ggAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACAt                                        <  2:6917090/151‑1 (MQ=17)

                                                                                                           ggAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACAt                                        <  1:2631609/151‑1 (MQ=17)

                                                                                                            gAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATc                                       <  2:8420535/151‑1 (MQ=17)

                                                                                                            gAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATc                                       <  1:221243/151‑1 (MQ=17)

                                                                                                             aaGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCg                                      <  2:4529394/151‑1 (MQ=17)

                                                                                                              aGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGa                                     <  2:5555309/151‑1 (MQ=17)

                                                                                                              aGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGa                                     <  2:1342766/151‑1 (MQ=17)

                                                                                                               gTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCATACCACGATGAGCTACATCGAg                                    >  2:3982851/1‑151 (MQ=12)

                                                                                                               gTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAg                                    <  1:4703330/151‑1 (MQ=17)

                                                                                                               gTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAg                                    >  2:3042400/1‑151 (MQ=17)

                                                                                                               gTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAg                                    <  2:10052777/151‑1 (MQ=17)

                                                                                                                ttGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAgg                                   <  1:9371327/151‑1 (MQ=17)

                                                                                                                  gACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGcc                                 <  1:6550810/151‑1 (MQ=17)

                                                                                                                    ccGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGa                               >  1:7654983/1‑151 (MQ=17)

                                                                                                                      gagaAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACt                             >  2:4318604/1‑151 (MQ=17)

                                                                                                                       agaAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACAGTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACTa                            <  2:11336264/151‑1 (MQ=12)

                                                                                                                        gaAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACTAc                           <  2:9597139/151‑1 (MQ=17)

                                                                                                                        gaAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACTAc                           >  1:7804474/1‑151 (MQ=17)

                                                                                                                          aGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACTACGa                         <  2:9723228/151‑1 (MQ=17)

                                                                                                                            gtaggggAGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACTACGATc                       <  1:3052122/148‑1 (MQ=255)

                                                                                                                            gTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACTACGATc                       >  1:6625196/1‑151 (MQ=18)

                                                                                                                               ggggTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACTACGATcaca                    >  1:1476043/1‑151 (MQ=21)

                                                                                                                                  gTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACTACGATCACATGc                 >  2:9809043/1‑151 (MQ=21)

                                                                                                                                   tGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACTACGATCACATGcg                <  2:5629099/151‑1 (MQ=21)

                                                                                                                                   tGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACTACGATCACATGcg                <  1:1806545/151‑1 (MQ=21)

                                                                                                                                     cGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACTACGATCACATGcgtg              >  1:1445983/1‑151 (MQ=21)

                                                                                                                                     cGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACTACGATCACATGcgtg              <  1:1076567/151‑1 (MQ=21)

                                                                                                                                      ggATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACTACGATCACATGcgtgc             >  1:11297762/1‑151 (MQ=21)

                                                                                                                                      ggATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACTACGATCACATGcgtgc             <  1:10078091/151‑1 (MQ=21)

                                                                                                                                         tGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACTACGATCACATGcgtgccgt          <  1:7818395/151‑1 (MQ=21)

                                                                                                                                           aCCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACTACGATCACATGcgtgccgtgc        >  1:1287890/1‑151 (MQ=21)

                                                                                                                                           aCCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACTACGATCACATGcgtgccgtgc        <  1:6331538/151‑1 (MQ=21)

                                                                                                                                              ccGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACTACGATCACATGCGTGCCGtgctgc     >  2:3698708/1‑151 (MQ=17)

                                                                                                                                                 cACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACTACGATCACATGCGTGCCGTGCTGCATg  >  1:5383098/1‑151 (MQ=17)

                                                                                                                                                 cACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACTACGATCACATGCGTGCCGTGCTGCATg  <  1:3855525/151‑1 (MQ=17)

CGATAGAGCCGGATTCGCCGATGACGACCAGTTGTTCAACGTCAACCGGTTCTCACCGCACTACAAGAGCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCGTTTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACTACGATCACATGCGTGCCGTGCTGCATG  >  NC_008463/5358360‑5358656

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`