BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_008463	559,051	+T	intergenic (+619/+70)	PA14_RS02545 → / ← rarD	glycine cleavage system protein R/EamA family transporter RarD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_008463	559,050	1	.	T	97.9%	1592.0 / 19.5	388	intergenic (+618/+71)	PA14_RS02545/rarD	glycine cleavage system protein R/EamA family transporter RarD
Reads supporting (aligned to +/- strand): ref base . (7/1); new base T (198/178); total (207/181)
Fisher's exact test for biased strand distribution p-value = 7.25e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 406 total aligned reads displayed.

ACAGCGGTGTGCTCAAAGACTGATCAGGCGCTTTGCAGGGCTTCTTCCGACGAGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCA‑GT‑A‑T‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCCGGCTGGGGAAGGCGGCGCAGGCTGCGCCAGGCGTCGAAGCTGTAGACGGCCAGGGCGAGCCAGATGAA  >  NC_008463/558900‑559195

aCAGCGGTGTGCTCAAAGACTGATCAGGCGCTTTGCAGGGCTTCTTCCGACGAGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCA‑GT‑a                                                                                                                                                     <  1:6075145/151‑1 (MQ=255)

   gCGGTGTGCTCAAAGACTGATCAGGCGCTTTGCAGGGCTTCTTCCGACGAGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCA‑GT‑A‑ttg                                                                                                                                                 <  1:2839854/151‑3 (MQ=255)

   gCGGTGTGCTCAAAGACTGATCAGGCGCTTTGCAGGGCTTCTTCCGACGAGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCA‑GT‑A‑ttg                                                                                                                                                 <  2:7085773/151‑3 (MQ=255)

        gtgCTCAAAGACTGATCAGGCGCTTTGCAGGGCTTCTTCCGACGAGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCA‑GT‑ATT‑GGCggg                                                                                                                                            >  2:5113854/1‑151 (MQ=255)

            tCAAAGACTGATCAGGCGCTTTGCAGGGCTTCTTCCGACGAGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCA‑GT‑ATT‑GGCGGGGGAt                                                                                                                                        >  1:2661540/1‑151 (MQ=255)

            tCAAAGACTGATCAGGCGCTTTGCAGGGCTTCTTCCGACGAGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGGCA‑GTCT‑T‑GGCGGGGGAt                                                                                                                                        >  2:7952556/1‑151 (MQ=255)

             cAAAGACTGATCAGGCGCTTTGCAGGGCTTCTTCCGACGAGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCA‑GT‑ATT‑GGCGGGGGATa                                                                                                                                       >  2:918080/1‑151 (MQ=255)

               aaGACTGATCAGGCGCTTTGCAGGGCTTCTTCCGACGAGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAGAAGCCTGGGGTGGATCGCTGAGCGTCA‑GT‑ATT‑GGCGGGGGATagg                                                                                                                                     >  2:1808829/1‑151 (MQ=255)

                  aCTGATCAGGCGCTTTGCAGGGCTTCTTCCGACGAGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCA‑GT‑ATT‑GGCGGGGGATaggaag                                                                                                                                  >  1:11379177/1‑151 (MQ=255)

                   cTGATCAGGCGCTTTGCAGGGCTTCTTCCGACGAGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCA‑GT‑ATT‑GGCGGGGGATaggaagg                                                                                                                                 <  2:5002464/151‑1 (MQ=255)

                   cTGATCAGGCGCTTTGCAGGGCTTCTTCCGACGAGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCA‑GT‑ATT‑GGCGGGGGATaggaagg                                                                                                                                 >  1:612115/1‑151 (MQ=255)

                    tGATCAGGCGCTTTGCAGGGCTTCTTCCGACGAGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCA‑GT‑ATT‑GGCGGGGGATaggaagga                                                                                                                                >  2:3175936/1‑151 (MQ=255)

                    tGATCAGGCGCTTTGCAGGGCTTCTTCCGACGAGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCA‑GT‑ATT‑GGCGGGGGATaggaagga                                                                                                                                <  1:8649472/151‑1 (MQ=255)

                     gATCAGGCGCTTTGCAGGGCTTCTTCCGACGAGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCA‑GT‑ATT‑GGCGGGGGATAGGAAGGAg                                                                                                                               <  1:1480784/151‑1 (MQ=255)

                     gATCAGGCGCTTTGCAGGGCTTCTTCCGACGAGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCA‑GT‑ATT‑GGCGGGGGATAGGAAGGAg                                                                                                                               <  2:10796514/151‑1 (MQ=255)

                      aTCAGGCGCTTTGCAGGGCTTCTTCCGACGAGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCA‑GT‑ATT‑GGCGGGGGATAGGAAGGAGt                                                                                                                              <  2:10722401/151‑1 (MQ=255)

                       tCAGGCGCTTTGCAGGGCTTCTTCCGACGAGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCA‑GT‑ATT‑GGCGGGGGATAGGAAGGAGTg                                                                                                                             >  2:7197254/1‑151 (MQ=255)

                              cTTTGCAGGGCTTCTTCCGACGAGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCA‑GT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGttt                                                                                                                      >  1:7826392/1‑151 (MQ=255)

                              cTTTGCAGGGCTTCTTCCGACGAGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCA‑GT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGttt                                                                                                                      >  1:8148594/1‑151 (MQ=255)

                              cTTTGCAGGGCTTCTTCCGACGAGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCA‑GT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGttt                                                                                                                      >  1:9928179/1‑151 (MQ=255)

                                ttGCAGGGCTTCTTCCGACGAGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCA‑GT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGttttt                                                                                                                    <  2:2535238/151‑1 (MQ=255)

                                  gCAGGGCTTCTTCCGACGAGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCA‑GT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTc                                                                                                                  >  2:9575667/1‑151 (MQ=255)

                                    aGGGCTTCTTCCGACGAGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCA‑GT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGc                                                                                                                >  2:10667818/1‑151 (MQ=255)

                                    aGGGCTTCTTCCGACGAGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCA‑GT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGc                                                                                                                >  1:10778430/1‑151 (MQ=255)

                                     gggCTTCTTCCGACGAGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCA‑GT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGcc                                                                                                               <  1:8569611/151‑1 (MQ=255)

                                     gggCTTCTTCCGACGAGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCA‑GT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGcc                                                                                                               <  1:8818146/151‑1 (MQ=255)

                                       gCTTCTTCCGACGAGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCA‑GT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAg                                                                                                             <  1:6700142/151‑1 (MQ=255)

                                        cttcttCCGACGAGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCA‑GT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAgg                                                                                                            <  2:2152761/151‑1 (MQ=255)

                                        cttcttCCGACGAGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCA‑GT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAgg                                                                                                            <  1:7519644/151‑1 (MQ=255)

                                          tcttcCGACGAGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCA‑GT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCt                                                                                                          <  1:7418082/151‑1 (MQ=255)

                                           cttcCGACGAGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCA‑GT‑AGT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCtt                                                                                                         >  1:2526101/1‑151 (MQ=255)

                                               cgacgaGTTATCCACGCGCCTGCGGGGCGGAGAAGGCAGGTCTATGCACAAAGTACGTGCCTGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCA‑GT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCgg                                                                                                     >  2:8258226/1‑151 (MQ=255)

                                                gacgaGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCA‑GT‑ATT‑GGCGGGGGATAGGAAGGAGGGCGTGTTTTTTCGCCAGGCTTGCGGa                                                                                                    >  2:624014/1‑151 (MQ=255)

                                                gacgaGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCA‑GG‑A‑TGGGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGa                                                                                                    >  2:5241283/1‑151 (MQ=255)

                                                    aGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCA‑GT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTc                                                                                                >  2:4312296/1‑151 (MQ=255)

                                                      ttATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCA‑GT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCg                                                                                              <  2:9086195/151‑1 (MQ=255)

                                                       tATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCA‑GT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGc                                                                                             <  2:11433651/151‑1 (MQ=255)

                                                           cACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCA‑GT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGc                                                                                         <  1:1985124/151‑1 (MQ=255)

                                                             cgcgCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCA‑GT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCt                                                                                       <  2:3025325/151‑1 (MQ=255)

                                                              gcgcCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCA‑GT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTg                                                                                      <  2:6953911/151‑1 (MQ=255)

                                                              gcgcCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCA‑GG‑ATT‑GGCGGGGGATAGGAGGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTg                                                                                      >  1:4471497/1‑151 (MQ=255)

                                                               cgcCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCA‑GT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTgg                                                                                     >  1:5544180/1‑151 (MQ=255)

                                                               cgcCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGGGGATCGCTGAGCGTCA‑GT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTng                                                                                     <  2:3899834/151‑1 (MQ=255)

                                                                gcCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCA‑GT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTggg                                                                                    >  1:1251880/1‑151 (MQ=255)

                                                                gcCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCA‑GT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTggg                                                                                    <  2:3118618/151‑1 (MQ=255)

                                                                 ccTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCA‑GT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTgggg                                                                                   >  1:10300720/1‑151 (MQ=255)

                                                                    gCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCA‑GT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATa                                                                                <  1:9228654/151‑1 (MQ=255)

                                                                     cGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCA‑GT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATaa                                                                               >  2:7662242/1‑151 (MQ=255)

                                                                     cGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCA‑GT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATaa                                                                               <  2:10137600/151‑1 (MQ=255)

                                                                      ggggAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGCACCTGTGGAATAGCCTGGGGTGGATCGCTGAGCGTCA‑GT‑ATT‑GGCGGGGGATAGGATGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAAc                                                                              >  2:11196909/1‑151 (MQ=255)

                                                                      ggggAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCA‑GT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAAc                                                                              >  2:4068305/1‑151 (MQ=255)

                                                                            gagaAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCA‑GT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGc                                                                        >  2:7475920/1‑151 (MQ=255)

                                                                            gagaAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCGGGGGTGGATCGCTGAGCGTCA‑GT‑ATT‑GGCGGGGGAGAGGTAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATCACGTCAGc                                                                        >  2:4635213/1‑151 (MQ=255)

                                                                             agaAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCA‑GT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGcc                                                                       >  1:5876400/1‑151 (MQ=255)

                                                                              gaAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCA‑GT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGccc                                                                      <  1:4071960/151‑1 (MQ=255)

                                                                                    aGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCA‑GT‑ATT‑GGCGGGGGATAGGAAGGAGCGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCCGGCTgg                                                                <  2:7820313/151‑1 (MQ=255)

                                                                                     ggTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCA‑GT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCCGGCTggg                                                               >  2:5860263/1‑151 (MQ=255)

                                                                                      gTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCA‑GT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCCGGCTgggg                                                              >  1:164337/1‑151 (MQ=255)

                                                                                      gTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCA‑GT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCCGGCTgggg                                                              >  1:8020882/1‑151 (MQ=255)

                                                                                       tCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCA‑GT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCCGGCTGGGGa                                                             <  1:962743/151‑1 (MQ=255)

                                                                                          aTGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCA‑GT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCCGGCTGGGGAAgg                                                          <  2:10906997/151‑1 (MQ=255)

                                                                                           tGCACACAGTACGTGCATGAACCTGTGGAAACGCCTGGGGTGGATCGCTGAGCGTCA‑GT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCAGGCTGGGGAAggc                                                         <  2:2680228/151‑1 (MQ=255)

                                                                                           tGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCA‑GT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCCGGCTGGGGAAggc                                                         <  2:2919306/151‑1 (MQ=255)

                                                                                           tGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCA‑GT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCCGGCTGGGGAAggc                                                         >  1:2185019/1‑151 (MQ=255)

                                                                                                 aaGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCA‑GT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCCGGCTGGGGAAGGCGGCGCa                                                   >  2:4381240/1‑151 (MQ=255)

                                                                                                  aGTACGTGCAAGAACCTGTGGAAAAGACTGGGGTGGATCGCTGAGCGTCACGA‑T‑T‑GGCGGGGGATCGCAAGCAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGATCCGCAAGCCTGGGGATAACGTCAGCCCGGCTGGGGAAGGCGGCGAAg                                                  >  1:11276745/1‑151 (MQ=255)

                                                                                                   gTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCA‑GT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCCGGCTGGGGAAGGCGGCGCAgg                                                 >  1:10949877/1‑151 (MQ=255)

                                                                                                    tACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCA‑GT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCCGGCTGGGGAAGGCGGCGCAGGc                                                <  1:6310095/151‑1 (MQ=255)

                                                                                                     aCGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCA‑GT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCCGGCTGGGGAAGGCGGCGCAGGCt                                               >  2:3709390/1‑151 (MQ=255)

                                                                                                       gTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCA‑GT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCCGGCTGGGGAAGGCGGCGCAGGCTgc                                             <  2:9278730/151‑1 (MQ=255)

                                                                                                        tGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCA‑GT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCCGGCTGGGGAAGGCGGCGCAGGCTgcg                                            <  1:10454476/151‑1 (MQ=255)

                                                                                                           aTGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCA‑GG‑ATT‑GGCGGGGGATCGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCCGGCTGGGGAAGGCGGCGCAGGCTGCGCCa                                         <  2:5589196/151‑1 (MQ=255)

                                                                                                            tGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCA‑GT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCCGGCTGGGGAAGGCGGCGCAGGCTGCGCCAg                                        >  1:898732/1‑151 (MQ=255)

                                                                                                              aaCCTGTGGAAACGCCTGGGGTGGATCGCTGAGCGTCA‑GG‑ATT‑GGCGGGGGATAGGTCGGAGGGCGGGTTTTTTCGCCAGGCTTGCGGAGTGCCGCAAGCCTGGGGGTGACGTCAGCCCGGCGGGGGAATGCGGCGCAGGCTGCGCCAgtc                                      >  2:6768529/1‑149 (MQ=255)

                                                                                                                ccTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCA‑GT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCCGGCTGGGGAAGGCGGCGCAGGCTGCGCCAGGCGt                                    >  1:3625309/1‑151 (MQ=255)

                                                                                                                 cTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCA‑GT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCCGGCTGGGGAAGGCGGCGCAGGCTGCGCCAGGCGTc                                   <  1:10150175/151‑1 (MQ=255)

                                                                                                                 cTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCA‑GT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCCGGCTGGGGAAGGCGGCGCAGGCTGCGCCAGGCGTc                                   >  1:1114060/1‑151 (MQ=255)

                                                                                                                  tgtgGAAAAGCCTGGGGTGGATCGCTGAGCGTCA‑GT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCCGGCTGGGGAAGGCGGCGCAGGCTGCGCCAGGCGTCg                                  <  1:3426996/151‑1 (MQ=255)

                                                                                                                   gtgGAAAAGCCTGGGGTGGATCGCTGAGCGTCA‑GT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCCGGCTGGGGAAGGCGGCGCAGGCTGCGCCCGGCGTCGa                                 >  1:7148014/1‑151 (MQ=255)

                                                                                                                    tgGAAAAGCCTGGGGTGGATCGCTGAGCGTCA‑GT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCCGGCTGGGGAAGGCGGCGCAGGCTGCGCCAGGCGTCGaa                                >  1:181912/1‑151 (MQ=255)

                                                                                                                       aaaaGCCTGGGGTGGATCGCTGAGCGGCA‑GT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCACGCCTGGGGATAACGTCAGCCCGGCTGGGGAAGGCGGCGCAGGCTGCGCCAGGCGTCGAAGCt                             >  2:4855144/1‑151 (MQ=255)

                                                                                                                        aaaGCCTGGGGTGGATCGCTGAGCGTCA‑GT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCCGGCTGGGGAAGGCGGCGCAGGCTGCGCCAGGCGTCGAAGCTg                            >  1:5423100/1‑151 (MQ=255)

                                                                                                                        aaaGCCTGGGGTGGATCGCTGAGCGTCA‑GT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCCGGCTGGGGAAGGCGGCGCAGGCTGCGCCAGGCGTCGAAGCTg                            >  2:10515395/1‑151 (MQ=255)

                                                                                                                          aGCCTGGGGTGGATCGCTGAGCGTCA‑GT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGATTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCCGGCTGGGGAAGGCGGCGCAGGCTGCGCCAGGCGTCGAAGCTGTa                          <  2:4740840/151‑1 (MQ=255)

                                                                                                                            ccTGGGGTGGATCGCTGAGCGTCA‑GT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCCGGCTGGGGAAGGCGGCGCAGGCTGCGCCAGGCGTCGAAGCTGTAGa                        >  1:84099/1‑151 (MQ=255)

                                                                                                                            ccTGGGGTGGATCGCTGAGCGTCA‑GT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCCGGCTGGGGAAGGCGGCGCAGGCTGCGCCAGGCGTCGAAGCTGTAGa                        >  1:4280206/1‑151 (MQ=255)

                                                                                                                               ggggTGGATCGCTGAGCGTCA‑GT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCCGGCTGGGGAAGGCGGCGCAGGCTGCGCCAGGCGTCGAAGCTGTAGACgg                     <  1:9303765/151‑1 (MQ=255)

                                                                                                                                 ggTGGATCGCTGAGCGTCA‑GT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCCGGCTGGGGAAGGCGGCGCAGGCTGCGCCAGGCGTCGAAGCTGTAGACGGcc                   >  1:5093503/1‑151 (MQ=255)

                                                                                                                                  gTGGATCGCTGAGCGTCA‑GT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCCGGCTGGGGAAGGCGGCGCAGGCTGCGCCAGGCGTCGAAGCTGTAGACGGCCa                  >  2:5386541/1‑151 (MQ=255)

                                                                                                                                    ggATCGCTGAGCGTCA‑GT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCCGGCTGGGGAAGGCGGCGCAGGCTGCGCCAGGCGTCGAAGCTGTAGACGGCCAgg                >  2:3389247/1‑151 (MQ=255)

                                                                                                                                      aTCGCTGAGCGTCA‑GT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCCGGCTGGGGAAGGCGGCGCAGGCTGCGCCAGGCGTCGAAGCTGTAGACGGCCAGGGc              <  2:94244/151‑1 (MQ=255)

                                                                                                                                        cGCTGAGCGTCA‑GT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCCGGCTGGGGAAGGCGGCGCAGGCTGCGCCAGGCGTCGAAGCTGTAGACGGCCAGGGCGa            >  2:8734705/1‑151 (MQ=255)

                                                                                                                                        cGCTGAGCGTCA‑GG‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCGTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCCGGCTGGGGCAGGCGGCGCAGGCTGCGCCAGGCGTCGAAGCTGTAGACGGCCAGGGCGa            <  2:9013922/151‑1 (MQ=255)

                                                                                                                                         gCTGAGCGTCA‑GT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCCGGCTGGGGAAGGCGGCGCAGGCTGCGCCAGGCGTCGAAGCTGTAGACGGCCAGGGCGAg           <  1:3107485/151‑1 (MQ=255)

                                                                                                                                             aGCGTCA‑GT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCCGGCTGGGGAAGGCGGCGCAGGCTGCGCCAGGCGTCGAAGCTGTAGACGGCCAGGGCGAGCCAg       >  2:7339511/1‑151 (MQ=255)

                                                                                                                                              gCGTCA‑GT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCCGGCTGGGGAAGGCGGCGCAGGCTGCGCCAGGCGTCGAAGCTGTAGACGGCCAGGGCGAGCCAGa      >  1:2041170/1‑151 (MQ=255)

                                                                                                                                               cGTCA‑GT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGTGGATAACGTCAGCACGGCTGGGGAATGCGGCGCAGGCTGCGCCAGGCGTCGAAGCTGTAGACGGCCAGGGCGAGCCAGAt     >  2:1422415/1‑151 (MQ=255)

                                                                                                                                               cGTCA‑GT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCCGGCTGGGGAAGGCGGCGCAGGCTGCGCCAGGCGTCGAAGCTGTAGACGGCCAGGGCGAGCCAGAt     <  2:4198692/151‑1 (MQ=255)

                                                                                                                                                 tCA‑GT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCCGGCTGGGGAAGGCGGCGCAGGCTGCGCCAGGCGTCGAAGCTGTAGACGGCCAGGGCGAGCCAGATGa   <  1:9473766/151‑1 (MQ=255)

                                                                                                                                                 tCA‑GT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCCGGCTGGGGAAGGCGGCGCAGGCTGCGCCAGGCGTCGAAGCTGTAGACGGCCAGGGCGAGCCAGATGa   >  1:6938497/1‑151 (MQ=255)

ACAGCGGTGTGCTCAAAGACTGATCAGGCGCTTTGCAGGGCTTCTTCCGACGAGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCA‑GT‑A‑T‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCCGGCTGGGGAAGGCGGCGCAGGCTGCGCCAGGCGTCGAAGCTGTAGACGGCCAGGGCGAGCCAGATGAA  >  NC_008463/558900‑559195

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`