Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 2,807,266 G→C V137L (GTG→CTG)  PA14_RS13175 → leucine‑rich repeat‑containing protein kinase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084632,807,2660GC99.8% 1989.1 / NA 504V137L (GTG→CTG) PA14_RS13175leucine‑rich repeat‑containing protein kinase family protein
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base C (256/247);  total (257/247)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 520 total aligned reads displayed.

GGCCGACTCCCTGGAGGTGCTCAACCTCAGCGGCAACGCCCTGGACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGGTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGCCCGAGGAACTGGGTCGCCGGCCGTTGCAGAAGCTGATGC  >  NC_008463/2807118‑2807413
                                                                                                                                                    |                                                                                                                                                   
ggCCGACTCCCTGGAGGTGCTCAACCTCAGCGGCAACGCCCTGGACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTg                                                                                                                                                   >  1:1095480/1‑151 (MQ=255)
 gCCGACTCCCTGGAGGTGCTCAACCTCAGCGGCAACGCCCTGGACTGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGa                                                                                                                                                  <  2:4573103/151‑1 (MQ=255)
  ccGACTCCCTGGAGGTGCTCAACCTCAGCGGCAACGCCCTGGACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAg                                                                                                                                                 >  1:847740/1‑151 (MQ=255)
  ccGACTCCCTGGAGGTGCTCAACCTCAGCGGCAACGCCCTGGACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAg                                                                                                                                                 >  1:2011945/1‑151 (MQ=255)
   cGACTCCCTGGAGGTGCTCAACCTCAGCGGCAACGCCCTGGACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGc                                                                                                                                                <  2:9343166/151‑1 (MQ=255)
    gACTCCCTGGAGGTGCTCAACCTCAGCGGCAACGCCCTGGACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGATGTTCTGTTCCGCCACCGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCa                                                                                                                                               >  2:8490186/1‑151 (MQ=255)
      cTCCCTGGAGGTGCTCAACCTCAGCGGCAACGCCCTGGACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGGTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATg                                                                                                                                             <  2:10177415/151‑1 (MQ=255)
       tCCCTGGAGGTGCTCAACCTCAGCGGCAACGCCCTGGACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATgg                                                                                                                                            <  2:6530529/151‑1 (MQ=255)
       tCCCTGGAGGTGCTCAACCTCAGCGGCAACGCCCTGGACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATgg                                                                                                                                            >  1:1276995/1‑151 (MQ=255)
       tCCCTGGAGGTGCTCAACCTCAGCGGCAACGCCCTGGACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATgg                                                                                                                                            >  1:1542563/1‑151 (MQ=255)
        cccTGGAGGTGCTCAACCTCAGCGGCAACGCCCTGGACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGt                                                                                                                                           <  1:7603436/151‑1 (MQ=255)
          cTGGAGGTGCTCAACCTCAGCGGCAACGCCCTGGACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCg                                                                                                                                         <  2:4984001/151‑1 (MQ=255)
           tGGAGGTGCTCAACCTCAGCGGCAACGCCCTGGACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCgg                                                                                                                                        <  1:10014295/151‑1 (MQ=255)
            ggAGGTGCTCAACCTCAGCGGCAACGCCCTGGACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGc                                                                                                                                       <  1:10674818/151‑1 (MQ=255)
               ggTGCTCAACCTCAGCGGCAACGCCCTGGACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTc                                                                                                                                    <  1:4099920/151‑1 (MQ=255)
               ggTGCTCAACCTCAGCGGCAACGCCCTGGACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTc                                                                                                                                    >  1:1770896/1‑151 (MQ=255)
                gTGCTCAACCTCAGCGGCAACGCCCTGGACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCa                                                                                                                                   >  2:706276/1‑151 (MQ=255)
                  gCTCAACCTCAGCGGCAACGCCCTGGACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAg                                                                                                                                 >  1:3102245/1‑151 (MQ=255)
                    tCAACCTCAGCGGCAACGCCCTGGACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTc                                                                                                                               >  2:6885600/1‑151 (MQ=255)
                    tCAACCTCAGCGGCAACGCCCTGGACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTc                                                                                                                               <  2:3448822/151‑1 (MQ=255)
                         cTCAGCGGCAACGCCCTGGACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAAcc                                                                                                                          >  2:4368927/1‑151 (MQ=255)
                          tCAGCGGCAACGCCCTGGACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCg                                                                                                                         <  2:11048999/151‑1 (MQ=255)
                           cAGCGGCAACGCCCTGGACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGc                                                                                                                        >  1:11568532/1‑151 (MQ=255)
                              cGGCAACGCCCTGGACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATc                                                                                                                     <  1:800692/151‑1 (MQ=255)
                                gCAACGCCCTGGACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGa                                                                                                                   <  1:10602942/151‑1 (MQ=255)
                                  aaCGCCCTGGACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGc                                                                                                                 <  1:142122/151‑1 (MQ=255)
                                   aCGCCCTGGACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCg                                                                                                                <  1:6909349/151‑1 (MQ=255)
                                    cGCCCTGGACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCgg                                                                                                               >  2:284728/1‑151 (MQ=255)
                                     gCCCTGGACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCggg                                                                                                              >  2:8601161/1‑151 (MQ=255)
                                      cccTGGACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGt                                                                                                             >  1:2677934/1‑151 (MQ=255)
                                        cTGGACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTgc                                                                                                           >  2:7329111/1‑151 (MQ=255)
                                           gACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCACGTCCAACCGCATCGAGCGGGTgccgg                                                                                                        >  1:9856970/1‑151 (MQ=255)
                                           gACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTgccgg                                                                                                        <  2:10294500/151‑1 (MQ=255)
                                           gACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTATGTTCCGCCAACGACTTCGCCGATTTGCCGGCGGCGGTGGTCGAGTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTgcagg                                                                                                        <  2:6683603/151‑1 (MQ=255)
                                            aCAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTgccggc                                                                                                       <  2:11274233/151‑1 (MQ=255)
                                             cAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGTTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTgccggcc                                                                                                      <  2:681512/151‑1 (MQ=255)
                                             cAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTgccggcc                                                                                                      >  2:9024713/1‑151 (MQ=255)
                                              aGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTgccggccg                                                                                                     >  1:6032965/1‑151 (MQ=255)
                                                 cTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCgg                                                                                                  >  2:8185833/1‑151 (MQ=255)
                                                 cTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCgg                                                                                                  >  1:7796025/1‑151 (MQ=255)
                                                   gCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGcc                                                                                                <  2:2160683/151‑1 (MQ=255)
                                                   gCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGcc                                                                                                >  1:6954656/1‑151 (MQ=255)
                                                     cGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCCACGACGTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTGCAAGTCCAACCGCATCGTGCGGGTGCCGGCCGCGGCCCt                                                                                              >  2:8314707/1‑151 (MQ=255)
                                                        acgacCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTgcc                                                                                           >  1:7186834/1‑151 (MQ=255)
                                                        acgacCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTgcc                                                                                           <  1:591923/151‑1 (MQ=255)
                                                         cgacCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTgccg                                                                                          <  2:10405151/151‑1 (MQ=255)
                                                          gacCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTgccgc                                                                                         >  1:6371278/1‑151 (MQ=255)
                                                          gacCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTgccgc                                                                                         >  1:6140822/1‑151 (MQ=255)
                                                             cTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAAGTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCACCCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCgg                                                                                      <  2:7918869/151‑1 (MQ=255)
                                                                ggccggcTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGt                                                                                   >  1:11336467/1‑151 (MQ=255)
                                                                ggccggcTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGt                                                                                   >  2:4862169/1‑151 (MQ=255)
                                                                   cggcTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTgc                                                                                >  2:7397090/1‑151 (MQ=255)
                                                                       tGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCgctg                                                                            <  1:5314602/151‑1 (MQ=255)
                                                                       tGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGGCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCgctg                                                                            <  1:1387616/151‑1 (MQ=255)
                                                                          aTCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCgctggct                                                                         >  1:3176807/1‑151 (MQ=255)
                                                                          aTCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCgctggct                                                                         >  1:4441057/1‑151 (MQ=255)
                                                                           tCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCgctggctg                                                                        >  1:3886154/1‑151 (MQ=255)
                                                                            cGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGa                                                                       <  2:8723760/151‑1 (MQ=255)
                                                                             gTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGAt                                                                      >  2:5171871/1‑151 (MQ=255)
                                                                              tctcAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATc                                                                     <  1:4954682/151‑1 (MQ=255)
                                                                                tcAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCt                                                                   <  1:8250104/151‑1 (MQ=255)
                                                                                  aaGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCa                                                                 >  2:5703587/1‑151 (MQ=255)
                                                                                   aGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCAc                                                                >  1:10456630/1‑151 (MQ=255)
                                                                                      tGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGa                                                             >  1:7538549/1‑151 (MQ=255)
                                                                                            tctgttcCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCg                                                       >  2:149657/1‑151 (MQ=255)
                                                                                             ctgttcCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCTACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCgg                                                      >  2:1359167/1‑151 (MQ=255)
                                                                                               gttcCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGAt                                                    <  1:2297442/151‑1 (MQ=255)
                                                                                               gttcCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGACCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCTAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCACCACCGACAAACGTAt                                                    >  2:1945241/1‑151 (MQ=255)
                                                                                                ttcCGCCAACGACGTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGAtt                                                   <  2:6081222/151‑1 (MQ=255)
                                                                                                 tcCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTg                                                  >  1:1026800/1‑151 (MQ=255)
                                                                                                  ccGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGc                                                 >  1:3665306/1‑151 (MQ=255)
                                                                                                      cAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACg                                             >  2:4708345/1‑151 (MQ=255)
                                                                                                      cAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACg                                             >  1:4713888/1‑151 (MQ=255)
                                                                                                            cTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGccc                                       >  2:5521278/1‑151 (MQ=255)
                                                                                                            cTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGccc                                       >  2:10790474/1‑151 (MQ=255)
                                                                                                            cTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGccc                                       <  1:4171584/151‑1 (MQ=255)
                                                                                                                      ttGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGCCCGAGGAACTgg                             >  1:5881921/1‑151 (MQ=255)
                                                                                                                        gCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGCCCGAGGAACTGGGt                           <  1:2082225/151‑1 (MQ=255)
                                                                                                                         ccGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGCCCGAGGAACTGGGTc                          <  2:2006384/151‑1 (MQ=255)
                                                                                                                          cggcggcggTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGCCCGAGGACCTGGGTCg                         >  2:6183624/1‑151 (MQ=255)
                                                                                                                          cggcggcggTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGCCCGAGGAACTGGGTCg                         <  2:9152114/151‑1 (MQ=255)
                                                                                                                           ggcggcggTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGCCCGAGGAACTGGGTCgc                        >  2:11424184/1‑151 (MQ=255)
                                                                                                                              ggcggTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGCCCGAGGACCTGGGTCgccgg                     >  2:9647849/1‑151 (MQ=255)
                                                                                                                              ggcggTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGCCCGAGGAACTGGGTCgccgg                     <  1:7898914/151‑1 (MQ=255)
                                                                                                                              ggcggTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGCCCGAGGAACTGGGTCgccgg                     >  1:9713542/1‑151 (MQ=255)
                                                                                                                                 ggTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGCCCGAGGAACTGGGTCgccggccg                  >  2:7502251/1‑151 (MQ=255)
                                                                                                                                   tGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGCCCGAGGAACTGGGTCGCCGGCCGtt                <  2:3165768/151‑1 (MQ=255)
                                                                                                                                    gggCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGCCCGAGGAACTGGGTCGCCGGCCGTTg               <  1:6648670/151‑1 (MQ=255)
                                                                                                                                     ggCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGCCCGAGGAACTGGGTCGCCGGCCGTTGc              <  1:8782742/151‑1 (MQ=255)
                                                                                                                                      gCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGCCCGAGGAACTGGGTCGCCGGCCGTTGCa             <  2:3799032/151‑1 (MQ=255)
                                                                                                                                        gACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGCCCGAGGAACTGGGTCGCCGGCCGTTGCAGa           >  2:7746694/1‑151 (MQ=255)
                                                                                                                                        gACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGCCCGAGGAACTGGGTCGCCGGCCGTTGCAGa           >  2:2371751/1‑151 (MQ=255)
                                                                                                                                         aCTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGCCCGAGGAACTGGGTCGCCGGCCGTTGCAGaa          <  2:4062865/151‑1 (MQ=255)
                                                                                                                                          cTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGCCCGAGGAACTGGGTCGCCGGCCGTTGCAGAAg         >  1:555852/1‑151 (MQ=255)
                                                                                                                                            gTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGCCCGAGGAACTGGGTCGCCGGCCGTTGCAGAAGCt       <  1:9219183/151‑1 (MQ=255)
                                                                                                                                             tCCGGCGCTGAGCATGGTCGGATACAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGCCCGAGGAACTGGGTCGCCGGCCGTTGCAGAAGCTg      >  2:9497146/1‑151 (MQ=255)
                                                                                                                                               cggcgcTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGCCCGAGGAACTGGGTCGCCGGCCGTTGCAGAAGCTGAt    <  2:8001880/151‑1 (MQ=255)
                                                                                                                                                ggcgcTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGCCCGAGGAACTGGGTCGCCGGCCGTTGCAGAAGCTGATg   >  1:2867285/1‑151 (MQ=255)
                                                                                                                                                ggcgcTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGCCCGAGGAACTGGGTCGCCGGCCGTTGCAGAAGCTGATg   >  1:8947630/1‑151 (MQ=255)
                                                                                                                                                ggcgcTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGCCCGAGGAACTGGGTCGCCGGCCGTTGCAGAAGCTGATg   >  1:8686324/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GGCCGACTCCCTGGAGGTGCTCAACCTCAGCGGCAACGCCCTGGACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGGTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGCCCGAGGAACTGGGTCGCCGGCCGTTGCAGAAGCTGATGC  >  NC_008463/2807118‑2807413

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑