Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 2,955,357 A→G L314L (TTG→CTG)  PA14_RS13700 ← PepSY domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084632,955,3570AG100.0% 1957.4 / NA 488L314L (TTG→CTG) PA14_RS13700PepSY domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (248/240);  total (248/240)

Only 100 of 488 total aligned reads displayed.

CAGATCAGCAGGCCGCTGAGCACCATCATGCTCAGCAGCAGGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAAGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCAGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAAGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCC  >  NC_008463/2955209‑2955504
                                                                                                                                                    |                                                                                                                                                   
cAGATCAGCAGGCCGCTGAGCACCATCATGCTCAGCAGCAGGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGc                                                                                                                                                   <  2:7478528/151‑1 (MQ=255)
   aTCAGCAGGCCGCTGAGCACCATCATGCTCAGCAGCAGGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCggg                                                                                                                                                <  1:10171450/151‑1 (MQ=255)
   aTCAGCAGGCCGCTGAGCACCATCATGCTCAGCAGCAGGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCggg                                                                                                                                                <  2:11505243/151‑1 (MQ=255)
   aTCAGCAGGCCGCTGAGCACCATCATGCTCAGCAGCAGGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCggg                                                                                                                                                <  2:4607078/151‑1 (MQ=255)
    tCAGCAGGCCGCTGAGCACCCTCATGCTCAGCAGCAGGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGggt                                                                                                                                               <  2:4731783/151‑1 (MQ=255)
    tCAGCAGGCCGCTGAGCACCATCATGCTCAGCAGCAGGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGggt                                                                                                                                               >  2:5148370/1‑151 (MQ=255)
     cagcagGCCGCTGAGCACCATCATGCTCAGCAGCAGGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGggtg                                                                                                                                              >  1:7835394/1‑151 (MQ=255)
      agcagGCCGCTGAGCACCATCATGCTCAGCAGCAGGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGggtgg                                                                                                                                             <  1:2581592/151‑1 (MQ=255)
       gcagGCCGCTGAGCACCATCATGCTCAGCAGCAGGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGggtggt                                                                                                                                            <  1:743359/151‑1 (MQ=255)
         agGCCGCTGAGCACCATCATGCTCAGCAGCAGGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGttt                                                                                                                                          >  2:287473/1‑151 (MQ=255)
         agGCCGCTGAGCACCATCATGCTCAGCAGCAGGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGttt                                                                                                                                          >  1:1777977/1‑151 (MQ=255)
          ggCCGCTGAGCACCATCATGCTCAGCAGCAGGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTc                                                                                                                                         <  1:4384324/151‑1 (MQ=255)
          ggCCGCTGAGCACCATCATGCTCAGCAGCAGGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTc                                                                                                                                         <  2:10261842/151‑1 (MQ=255)
          ggCCGCTGAGCACCATCATGCTCAGCAGCAGGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTc                                                                                                                                         <  1:8884486/151‑1 (MQ=255)
          cgCCGCTGAGCACCATCATGCTCAGCAGCAGGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTc                                                                                                                                         <  1:5191671/150‑1 (MQ=255)
               cTGAGCACCATCATGCTCAGCAGCAGGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCt                                                                                                                                    >  1:8284394/1‑151 (MQ=255)
                tGAGCACCATCATGCTCAGCAGCAGGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCtt                                                                                                                                   <  1:3530460/151‑1 (MQ=255)
                tGAGCACCATCATGCTCAGCAGCAGGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCtt                                                                                                                                   <  2:3583486/151‑1 (MQ=255)
                 gAGCACCATCATGCTCAGCAGCAGGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTg                                                                                                                                  >  2:321882/1‑151 (MQ=255)
                     aCCATCATGCTCAGCAGCAGGCCGAAGACGAACCAGATCAGCTTGACCCAGCTACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCgg                                                                                                                              <  1:3813307/151‑1 (MQ=255)
                      ccATCATGCTCAGCAGCAGGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGt                                                                                                                             <  1:10841227/151‑1 (MQ=255)
                       catcatGCTCAGCAGCAGGCCGAAGACGAACCAGATCAGCTTGACCCAGAGACCGCCGAACTCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGta                                                                                                                            <  2:7730858/151‑1 (MQ=255)
                           atGCTCAGCAGCAGGCCGAAGACGACCCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGAGCAGGGGACGCATGGATTCGGTGACGACCTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAg                                                                                                                        <  2:1921098/151‑1 (MQ=255)
                           atGCTCAGCAGCAGGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAg                                                                                                                        <  1:5686497/151‑1 (MQ=255)
                            tGCTCAGCAGCAGGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAgg                                                                                                                       <  1:10393738/151‑1 (MQ=255)
                                cagcagcagGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTAGAAGGGTTc                                                                                                                   >  2:4357300/1‑151 (MQ=255)
                                cagcagcagGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACAGCACGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCGGGTATAAGGGTTc                                                                                                                   >  2:7765746/1‑151 (MQ=255)
                                 agcagcagGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCa                                                                                                                  >  1:85456/1‑151 (MQ=255)
                                   cagcagGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAgc                                                                                                                <  2:5381955/151‑1 (MQ=255)
                                    agcagGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAgcg                                                                                                               >  2:5572590/1‑151 (MQ=255)
                                          ccGAAGACGACCCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCg                                                                                                         <  1:1584743/151‑1 (MQ=255)
                                          ccGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCg                                                                                                         <  1:7311672/151‑1 (MQ=255)
                                            gAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGAc                                                                                                       >  1:1240862/1‑151 (MQ=255)
                                             aaGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACt                                                                                                      <  2:10969993/151‑1 (MQ=255)
                                                aCGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGa                                                                                                   <  2:6162858/151‑1 (MQ=255)
                                                aCGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGa                                                                                                   <  1:9230150/151‑1 (MQ=255)
                                                 cGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGaa                                                                                                  >  1:11024423/1‑151 (MQ=255)
                                                  gAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAAc                                                                                                 >  2:2701978/1‑151 (MQ=255)
                                                  gAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAAc                                                                                                 <  1:904465/151‑1 (MQ=255)
                                                    aCCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACAt                                                                                               >  1:2295912/1‑151 (MQ=255)
                                                      cAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGa                                                                                             >  2:6748219/1‑151 (MQ=255)
                                                       aGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAg                                                                                            >  1:6435288/1‑151 (MQ=255)
                                                        gATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGc                                                                                           <  2:5613094/151‑1 (MQ=255)
                                                           cAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGa                                                                                        >  1:5073218/1‑151 (MQ=255)
                                                           cAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGa                                                                                        >  1:1989501/1‑151 (MQ=255)
                                                                   cccAGCTACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGcc                                                                                <  2:1953888/151‑1 (MQ=255)
                                                                   cccAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGcc                                                                                <  2:2338166/151‑1 (MQ=255)
                                                                    ccAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCcg                                                                               <  2:1513941/151‑1 (MQ=255)
                                                                       gATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGt                                                                            <  2:2220460/151‑1 (MQ=255)
                                                                         tACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTcc                                                                          <  2:370081/151‑1 (MQ=255)
                                                                           ccgccgAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTccgg                                                                        <  1:9347119/151‑3 (MQ=255)
                                                                             gccgAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGcc                                                                      >  2:1319295/1‑151 (MQ=255)
                                                                               cgAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCAc                                                                    <  1:3303990/151‑1 (MQ=255)
                                                                               cgAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCAc                                                                    <  1:5305802/151‑1 (MQ=255)
                                                                                  aaTCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGATCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGaa                                                                 >  2:8696243/1‑151 (MQ=255)
                                                                                     cGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACAt                                                              <  1:9629784/151‑1 (MQ=255)
                                                                                      gCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATc                                                             >  1:11378195/1‑151 (MQ=255)
                                                                                      gCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATc                                                             >  1:10927861/1‑151 (MQ=255)
                                                                                       ccGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCg                                                            <  2:9474202/151‑1 (MQ=255)
                                                                                        cGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCgg                                                           <  2:6559345/151‑1 (MQ=255)
                                                                                         ggTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCggg                                                          >  2:591793/1‑151 (MQ=255)
                                                                                         ggTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCggg                                                          >  1:1194446/1‑151 (MQ=255)
                                                                                          gtgtGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGc                                                         <  1:7443885/151‑1 (MQ=255)
                                                                                           tgtgCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCt                                                        >  1:6885570/1‑151 (MQ=255)
                                                                                             tgCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGt                                                      >  2:10457265/1‑151 (MQ=255)
                                                                                              gCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTa                                                     <  2:10096540/151‑1 (MQ=255)
                                                                                                    gACGCATGGATTCGGTGACGTACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGGCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGtt                                               <  2:9110786/151‑1 (MQ=255)
                                                                                                    gACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGtt                                               <  2:8105861/151‑1 (MQ=255)
                                                                                                     aCGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTg                                              >  1:5818669/1‑151 (MQ=255)
                                                                                                     aCGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTg                                              <  1:10075042/151‑1 (MQ=255)
                                                                                                       gCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGcc                                            >  1:669650/1‑151 (MQ=255)
                                                                                                         aTGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCgg                                          <  1:5566854/151‑1 (MQ=255)
                                                                                                           ggATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGc                                        <  1:10599082/151‑1 (MQ=255)
                                                                                                              ttCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCagg                                     >  2:3440166/1‑149 (MQ=255)
                                                                                                              ttCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCagg                                     <  2:7020145/151‑3 (MQ=255)
                                                                                                                 ggTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAac                                  >  2:8392840/1‑151 (MQ=255)
                                                                                                                 ggTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAac                                  <  1:7074921/151‑1 (MQ=255)
                                                                                                                    gACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACAcc                               <  1:8016963/151‑1 (MQ=255)
                                                                                                                     aCGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACcg                              <  1:10512037/151‑1 (MQ=255)
                                                                                                                       gAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACcgcg                            >  1:1501942/1‑151 (MQ=255)
                                                                                                                         aCTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGACCACCGCGCt                          >  2:11264206/1‑151 (MQ=255)
                                                                                                                         aCTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCt                          >  1:462054/1‑151 (MQ=255)
                                                                                                                         aCTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCt                          >  1:2091048/1‑151 (MQ=255)
                                                                                                                             cAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGcc                      <  1:10775579/151‑1 (MQ=255)
                                                                                                                              aGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCt                     <  2:7120948/151‑1 (MQ=255)
                                                                                                                                 ggggTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCa                  <  1:5001495/151‑1 (MQ=255)
                                                                                                                                 ggggTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCa                  >  1:11145491/1‑151 (MQ=255)
                                                                                                                                   ggTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAgg                >  1:6047435/1‑151 (MQ=255)
                                                                                                                                   ggTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAgg                <  2:2508333/151‑1 (MQ=255)
                                                                                                                                    gTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGc               <  1:1386599/151‑1 (MQ=255)
                                                                                                                                     tACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCt              >  2:8835746/1‑151 (MQ=255)
                                                                                                                                      tcGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTg             <  2:5543322/150‑1 (MQ=255)
                                                                                                                                      aCGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGTACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTg             <  2:10518451/151‑1 (MQ=255)
                                                                                                                                       cGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTgg            <  2:10825765/151‑1 (MQ=255)
                                                                                                                                        ggTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTggg           >  1:5486369/1‑151 (MQ=255)
                                                                                                                                        ggTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGATCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGTCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTggg           >  2:1612693/1‑151 (MQ=255)
                                                                                                                                         gTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATCAGGGTTCAGCGCGGCAGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGGTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTgggg          >  2:3053656/1‑151 (MQ=255)
                                                                                                                                             cTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATAc      >  1:2942657/1‑151 (MQ=255)
                                                                                                                                             cTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATAc      >  1:7609901/1‑151 (MQ=255)
                                                                                                                                              tGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACg     >  2:1067400/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CAGATCAGCAGGCCGCTGAGCACCATCATGCTCAGCAGCAGGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAAGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCAGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAAGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCC  >  NC_008463/2955209‑2955504

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑