Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 2,955,468 A→G L277L (TTG→CTG)  PA14_RS13700 ← PepSY domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084632,955,4680AG99.8% 1888.8 / NA 478L277L (TTG→CTG) PA14_RS13700PepSY domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (264/213);  minor base T (1/0);  total (265/213)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 478 total aligned reads displayed.

CGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAAGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCAGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAAGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTGCGGCGCGGGGCACGTCGGCGCGTGCGATGACCGGAGGGATGCCGTCGTTGGTCA  >  NC_008463/2955321‑2955614
                                                                                                                                                   |                                                                                                                                                  
cGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGaa                                                                                                                                                 <  1:1509552/151‑1 (MQ=255)
  gTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAaca                                                                                                                                               <  2:2896241/151‑1 (MQ=255)
   tGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAacac                                                                                                                                              <  2:5239891/151‑1 (MQ=255)
   tGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAacac                                                                                                                                              >  1:10995809/1‑151 (MQ=255)
     aCGACCTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACcg                                                                                                                                            <  2:3429672/151‑1 (MQ=255)
     aCGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACcg                                                                                                                                            >  2:4880412/1‑151 (MQ=255)
      cGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACcgc                                                                                                                                           <  2:11613988/151‑1 (MQ=255)
        aaCTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCgcgc                                                                                                                                         >  1:7470494/1‑151 (MQ=255)
          cTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCAGGGCAGGAACACCGCGCTg                                                                                                                                       >  2:634942/1‑151 (MQ=255)
           tGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTgg                                                                                                                                      >  2:2508973/1‑151 (MQ=255)
           tGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTgg                                                                                                                                      <  1:8448689/151‑1 (MQ=255)
           tGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTgg                                                                                                                                      <  1:10541256/151‑1 (MQ=255)
               ggggggTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTc                                                                                                                                  >  1:11149075/1‑151 (MQ=255)
                 ggggTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCa                                                                                                                                <  1:5001495/151‑1 (MQ=255)
                  gggTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAg                                                                                                                               <  1:7908832/151‑1 (MQ=255)
                     tACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCt                                                                                                                            >  1:1204662/1‑151 (MQ=255)
                     tACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCt                                                                                                                            >  2:8836387/1‑151 (MQ=255)
                      aCGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTg                                                                                                                           <  2:5543216/151‑1 (MQ=255)
                      aCGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTg                                                                                                                           >  2:7339616/1‑151 (MQ=255)
                        ggTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGACCACCGCGCTGGCCTCCAGGCTggg                                                                                                                         >  2:239614/1‑151 (MQ=255)
                        ggTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTggg                                                                                                                         >  1:5486369/1‑151 (MQ=255)
                        ggTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGATCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGTCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTggg                                                                                                                         >  2:1612693/1‑151 (MQ=255)
                            gCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATa                                                                                                                     >  1:10840534/1‑151 (MQ=255)
                            gCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATa                                                                                                                     >  2:3034831/1‑151 (MQ=255)
                             cTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATAc                                                                                                                    >  1:2942657/1‑151 (MQ=255)
                             cTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATAc                                                                                                                    >  2:10350818/1‑151 (MQ=255)
                             cTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATAc                                                                                                                    >  1:7609901/1‑151 (MQ=255)
                               gAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGc                                                                                                                  <  1:713875/151‑1 (MQ=255)
                                    ggCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGt                                                                                                             <  2:402392/150‑1 (MQ=255)
                                         gtggtTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGa                                                                                                        >  2:9898086/1‑151 (MQ=255)
                                          tggtTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAg                                                                                                       >  2:10962089/1‑151 (MQ=255)
                                          tggtTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAg                                                                                                       <  1:5331772/151‑1 (MQ=255)
                                            gtTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGcc                                                                                                     <  2:7268819/151‑1 (MQ=255)
                                              ttCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGc                                                                                                   >  2:1286474/1‑151 (MQ=255)
                                              ttCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGc                                                                                                   >  2:4456909/1‑151 (MQ=255)
                                              ttCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGc                                                                                                   >  1:8163388/1‑151 (MQ=255)
                                                cGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGACCATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCAc                                                                                                 >  2:8461609/1‑151 (MQ=255)
                                                 gATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCAcc                                                                                                >  2:3716437/1‑151 (MQ=255)
                                                 gATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCAcc                                                                                                <  2:11233168/151‑1 (MQ=255)
                                                 gATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCAcc                                                                                                <  1:6952417/151‑1 (MQ=255)
                                                  aTCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCg                                                                                               >  1:4607710/1‑151 (MQ=255)
                                                  aTCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCg                                                                                               >  1:9699640/1‑151 (MQ=255)
                                                    cTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGcc                                                                                             >  2:6946361/1‑151 (MQ=255)
                                                     ttGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCt                                                                                            <  1:193125/151‑1 (MQ=255)
                                                     gtGCCGGTATCAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCt                                                                                            <  1:5992108/150‑1 (MQ=255)
                                                      tGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTc                                                                                           <  2:4721202/151‑1 (MQ=255)
                                                        ccGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGt                                                                                         >  1:2800286/1‑151 (MQ=255)
                                                         cGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGGTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTc                                                                                        <  2:3517051/151‑1 (MQ=255)
                                                          ggTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTcc                                                                                       >  1:8957950/1‑151 (MQ=255)
                                                          ggTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTcc                                                                                       >  2:5900191/1‑151 (MQ=255)
                                                           gTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCa                                                                                      >  1:9959309/1‑151 (MQ=255)
                                                            tataAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAg                                                                                     >  1:107615/1‑151 (MQ=255)
                                                             ataAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGc                                                                                    >  2:6031805/1‑151 (MQ=255)
                                                                  ggTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGAt                                                                               <  2:4592360/151‑1 (MQ=255)
                                                                    tacAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCCAGTCCGGACACGAACATCGGGCTCTAGGAGTTCCCGGCCATGAACACCGCGCTGGCCTCCAGGCAGGGGATACGACCGGTGACGAGCCGAACCGCCACGACAAGCTCGATGt                                                                             >  2:6578217/3‑151 (MQ=255)
                                                                       aGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATg                                                                          >  2:1950000/1‑151 (MQ=255)
                                                                         cgcgGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGa                                                                        <  1:3957114/151‑1 (MQ=255)
                                                                          gcgGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGaa                                                                       >  2:4231612/1‑151 (MQ=255)
                                                                             gCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCt                                                                    >  2:10646641/1‑151 (MQ=255)
                                                                              ccGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCtt                                                                   >  2:9010947/1‑151 (MQ=255)
                                                                               cGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTc                                                                  <  1:129806/151‑1 (MQ=255)
                                                                               cGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTc                                                                  <  1:3398913/151‑1 (MQ=255)
                                                                                  cTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGa                                                               >  2:8313901/1‑151 (MQ=255)
                                                                                   tGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGAc                                                              >  2:9342619/1‑151 (MQ=255)
                                                                                    ggAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGAcc                                                             <  2:10904876/151‑1 (MQ=255)
                                                                                    ggAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGAcc                                                             <  1:5962666/151‑1 (MQ=255)
                                                                                     gAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCg                                                            <  1:220767/151‑1 (MQ=255)
                                                                                            aGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTgcgg                                                     >  2:2738236/1‑151 (MQ=255)
                                                                                            aGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTgcgg                                                     <  2:1568826/151‑1 (MQ=255)
                                                                                            aGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTgcgg                                                     >  2:1046923/1‑151 (MQ=255)
                                                                                             gCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTgcggc                                                    <  2:7492381/151‑1 (MQ=255)
                                                                                              cGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTgcggcg                                                   <  1:10238572/151‑1 (MQ=255)
                                                                                                     cAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTGCGGCGCGGGGCa                                            >  2:10173312/1‑151 (MQ=255)
                                                                                                     cAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTGCGGCGCGGGGCa                                            <  2:6719901/151‑1 (MQ=255)
                                                                                                      aGCCGCGTCCGGCCACGAACCTCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTGCGGCGCGGGGCAc                                           <  2:10103522/151‑1 (MQ=255)
                                                                                                        ccGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTGCGGCGCGGGGCACGt                                         <  2:9535726/151‑1 (MQ=255)
                                                                                                           cgTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTGCGGCGCGGGGCACGTCgg                                      >  1:10432237/1‑151 (MQ=255)
                                                                                                            gTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTGCGGCGCGGGGCACGTCGgc                                     <  2:85456/151‑1 (MQ=255)
                                                                                                            gTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTGCGGCGCGGGGCACGTCGgc                                     >  1:2480907/1‑151 (MQ=255)
                                                                                                            gTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTGCGGCGCGGGGCACGTCGgc                                     >  2:11497301/1‑151 (MQ=255)
                                                                                                            gTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTGCGGCGCGGGGCACGTCGgc                                     <  1:3655218/151‑1 (MQ=255)
                                                                                                             tCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTGCGGCGCGGGGCACGTCGgcg                                    >  2:2210331/1‑151 (MQ=255)
                                                                                                             tCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGAGGTATGGAACCTTCTGACCGGGTGCGGCGCGGGGCACGTCGgcg                                    >  2:8011620/1‑151 (MQ=255)
                                                                                                                   cACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTGCGGCGCGGGGCACGTCGGCGCGTGCg                              <  2:3167651/151‑1 (MQ=255)
                                                                                                                   cACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTGCGGCGCGGGGCACGTCGGCGCGTGCg                              >  1:3089324/1‑151 (MQ=255)
                                                                                                                    aCGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTGCGGCGCGGGGCACGTCGGCGCGTGCGa                             >  2:8100630/1‑151 (MQ=255)
                                                                                                                     cGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTGCGGCGCGGGGCACGTCGGCGCGTGCGAt                            >  1:8312549/1‑151 (MQ=255)
                                                                                                                      gAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTGCGGCGCGGGGCACGTCGGCGCGTGCGATg                           >  2:870436/1‑151 (MQ=255)
                                                                                                                      gAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTGCGGCGCGGGGCACGTCGGCGCGTGCGATg                           <  1:10157881/151‑1 (MQ=255)
                                                                                                                         cATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTGCGGCGCGGGGCACGTCGGCGCGTGCGATGAcc                        <  1:4699087/151‑1 (MQ=255)
                                                                                                                           tCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGTCGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTGCGGCGCGGGGCACGTCGGCGCGTGCGATGTCCgg                      <  2:7738574/151‑1 (MQ=255)
                                                                                                                            cGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTGCGGCGCGGGGCACGTCGGCGCGTGCGATGACCGGa                     >  2:9819768/1‑151 (MQ=255)
                                                                                                                             gggCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTGCGGCGCGGGGCACGTCGGCGCGTGCGATGACCGGAg                    >  1:6369141/1‑151 (MQ=255)
                                                                                                                                cTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTGCGGCGCGGGGCACGTCGGCGCGTGCGATGACCGGAGGGa                 >  1:11281482/1‑151 (MQ=255)
                                                                                                                                      gCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTGCGGCGCGGGGCACGTCGGCGCGTGCGATGACCGGAGGGATGCcgt           <  1:8548050/151‑1 (MQ=255)
                                                                                                                                        gTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTGCGGCGCGGGGCACGTCGGCGCGTGCGATGACCGGAGGGATGCcgtcg         >  1:4489380/1‑151 (MQ=255)
                                                                                                                                         ttGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTGCGGCGCGGGGCACGTCGGCGCGTGCGATGACCGGAGGGATGCcgtcgt        >  1:6695056/1‑151 (MQ=255)
                                                                                                                                            ccGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTGCGGCGCGGGGCACGTCGGCGCGTGCGATGACCGGAGGGATGCCGTCGTTgg     <  2:4050322/151‑1 (MQ=255)
                                                                                                                                            ccGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTGCGGCGCGGGGCACGTCGGCGCGTGCGATGACCGGAGGGATGCCGTCGTTgg     >  1:9365691/1‑151 (MQ=255)
                                                                                                                                               ggCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTGCGGCGCGGGGCACGTCGGCGCGTGCGATGACCGGAGGGATGCCGTCGTTGGTCa  <  2:10769407/151‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAAGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCAGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAAGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTGCGGCGCGGGGCACGTCGGCGCGTGCGATGACCGGAGGGATGCCGTCGTTGGTCA  >  NC_008463/2955321‑2955614

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑