Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 3,373,667 G→C V352V (GTC→GTG)  PA14_RS15385 ← aminotransferase class V‑fold PLP‑dependent enzyme

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084633,373,6670GC99.6% 1916.1 / NA 482V352V (GTC→GTG) PA14_RS15385aminotransferase class V‑fold PLP‑dependent enzyme
Reads supporting (aligned to +/- strand):  ref base G (2/0);  new base C (230/250);  total (232/250)
Fisher's exact test for biased strand distribution p-value = 2.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 484 total aligned reads displayed.

GAGGGCGGGTCGAGCCGGCCGCTCAGCCTGCCCTGGATACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGC‑GACCGAGGCTCAGGCGGACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTGGGGGACAAAGGCCGGGTTGTCGAAGGTC  >  NC_008463/3373519‑3373814
                                                                                                                                                     |                                                                                                                                                   
gAGGGCGGGTCGAGCCGGCCGCTCAGCCTGCCCTGGATACCCCGAGGACCGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGC‑GACCGAGGCTCAGGCGCAc                                                                                                                                                   >  2:7972045/1‑151 (MQ=255)
gAGGGCGGGTCGAGCCGGCCGCTCAGCCTGCCCTGGATACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGC‑GACCGAGGCTCAGGCGCAc                                                                                                                                                   <  2:10367763/151‑1 (MQ=255)
 aGGGCGGGTCGAGCCGGCCGCTCAGCCTGCCCTGGATACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGC‑GACCGAGGCTCAGGCGCACg                                                                                                                                                  <  1:6705250/151‑1 (MQ=255)
   ggCGGGTCGAGCCGGCCGCTCAGCCTGCCCTGGATACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGC‑GACCGAGGCTCAGGCGCACGCt                                                                                                                                                <  2:6887636/151‑1 (MQ=255)
   ggCGGGTCGAGCCGGCCGCTCAGCCTGCCCTGGATACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGC‑GACCGAGGCTCAGGCGCACGCt                                                                                                                                                >  1:1824208/1‑151 (MQ=255)
    gCGGGTCGAGCCGGCCGCTCAGCCTGCCCTGGATACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGC‑GACCGAGGCTCAGGCGCACGCTg                                                                                                                                               >  2:10037700/1‑151 (MQ=255)
       ggTCGAGCCGGCCGCTCAGCCTGCCCTGGATACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGC‑GACCGAGGCTCAGGCGCACGCTGCgg                                                                                                                                            >  2:9767357/1‑151 (MQ=255)
        gTCGAGCCGGCCGCTCAGCCTGCCCTGGATACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGC‑GACCGAGGCTCAGGCGCACGCTGCGGc                                                                                                                                           <  1:7000269/151‑1 (MQ=255)
           gAGCCGGCCGCTCAGCCTGCCCTGGATACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGC‑GACCGAGGCTCAGGCGCACGCTGCGGCCAg                                                                                                                                        >  2:10839392/1‑151 (MQ=255)
               cggccgCTCAGCCTGCCCTGGATACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGC‑GACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCt                                                                                                                                    >  1:5983581/1‑151 (MQ=255)
               cggccgCTCAGCCTGCCCTGGATACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGC‑GACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCt                                                                                                                                    >  2:2042754/1‑151 (MQ=255)
                ggccgCTCAGCCTGCCCTGGATACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGC‑GACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTg                                                                                                                                   <  1:8464154/151‑1 (MQ=255)
                ggccgCTCAGCCTGCCCTGGATACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGC‑GACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTg                                                                                                                                   <  2:3990014/151‑1 (MQ=255)
                  ccgCTCTGCCTGCCCTGGATACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGC‑GACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTggc                                                                                                                                 <  1:9440114/151‑1 (MQ=255)
                  ccgCTCAGCCTGCCCTGGATACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGC‑GACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTggc                                                                                                                                 <  2:1488064/151‑1 (MQ=255)
                   cgCTCAGCCTGCCCTGGATACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGC‑GACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTggcg                                                                                                                                <  2:1664501/151‑1 (MQ=255)
                    gCTCAGCCTGCCCTGGATACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGC‑GACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTggcgg                                                                                                                               <  2:787129/151‑1 (MQ=255)
                     cTCAGCCTGCCCTGGATACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGC‑GACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTggcgnc                                                                                                                              <  1:11379851/151‑1 (MQ=255)
                      tCAGCCTGCCCTGGATACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGC‑GACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCa                                                                                                                             <  1:9250297/151‑1 (MQ=255)
                        aGCCTGCCCTGGATACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGC‑GACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCCTc                                                                                                                           >  1:3996606/1‑151 (MQ=255)
                        aGCCTGCCCTGGATACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGC‑GACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATc                                                                                                                           >  1:5062732/1‑151 (MQ=255)
                              cccTGGATACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGC‑GACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGcc                                                                                                                     >  2:9557869/1‑151 (MQ=255)
                              cccTGGAGACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGC‑GACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGcc                                                                                                                     <  2:7798152/151‑1 (MQ=255)
                               ccTGGATACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGC‑GACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCg                                                                                                                    >  1:9941620/1‑151 (MQ=255)
                                cTGGATACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGC‑GACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGa                                                                                                                   >  1:11043891/1‑151 (MQ=255)
                                    aTACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGC‑GACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGc                                                                                                               <  2:9938239/151‑1 (MQ=255)
                                      aCCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGC‑GACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCga                                                                                                             >  2:3241390/1‑151 (MQ=255)
                                      aCCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGC‑GACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCga                                                                                                             <  1:7820096/151‑1 (MQ=255)
                                       ccccGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGC‑GACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCgag                                                                                                            <  1:10061187/151‑1 (MQ=255)
                                       ccccGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGC‑GACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCgag                                                                                                            <  1:7376504/151‑1 (MQ=255)
                                        cccGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGC‑GACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCgaga                                                                                                           >  1:5725784/1‑151 (MQ=255)
                                           gAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGGGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGC‑GACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGc                                                                                                        <  1:1075432/151‑1 (MQ=255)
                                            aGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGC‑GACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCa                                                                                                       <  2:11349433/151‑1 (MQ=255)
                                            aGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGC‑GACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCa                                                                                                       <  2:9070344/151‑1 (MQ=255)
                                               aaCGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGC‑GACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGt                                                                                                    >  1:10893561/1‑151 (MQ=255)
                                                  ggCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGC‑GACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTggg                                                                                                 <  1:587037/151‑1 (MQ=255)
                                                  ggCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGC‑GACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTggg                                                                                                 >  1:4496534/1‑151 (MQ=255)
                                                  ggCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGC‑GACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTggg                                                                                                 <  2:722808/151‑1 (MQ=255)
                                                  ggCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGC‑GACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTggg                                                                                                 >  1:754876/1‑151 (MQ=255)
                                                   gCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGC‑GACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGa                                                                                                >  2:10659431/1‑151 (MQ=255)
                                                     tGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGC‑GACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACg                                                                                              >  2:5586465/1‑151 (MQ=255)
                                                             cGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGC‑GACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCgccggc                                                                                      <  2:6579223/151‑1 (MQ=255)
                                                              ggCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGC‑GACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCgccggcc                                                                                     <  1:2070817/151‑1 (MQ=255)
                                                              ggCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGC‑GACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCgccggcc                                                                                     >  2:3811701/1‑151 (MQ=255)
                                                               gcgcgcCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGC‑GACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCgccggccg                                                                                    <  2:2207134/151‑1 (MQ=255)
                                                                cgcgcCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGC‑GACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCcgc                                                                                   >  1:8919961/1‑151 (MQ=255)
                                                                  cgcCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCACGCCGG‑GGAGCAGACCGAGGCACAGGCGCACGCTGCGGCCAGAGCTGGCGGCATCGTGGCCGAGGGCGTGAAGCACGTGGGACGGCGCCGGCCgcgc                                                                                 >  2:2608181/1‑151 (MQ=255)
                                                                    ccACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGC‑GACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCg                                                                               <  1:381896/151‑1 (MQ=255)
                                                                    ccACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGC‑GACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCg                                                                               >  2:11480412/1‑151 (MQ=255)
                                                                     cACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGC‑GACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGa                                                                              >  1:9155728/1‑151 (MQ=255)
                                                                      aCTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGC‑GACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAg                                                                             >  2:5159365/1‑151 (MQ=255)
                                                                      aCTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGC‑GACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAg                                                                             >  2:2922376/1‑151 (MQ=255)
                                                                       cTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGC‑GACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGt                                                                            <  2:8728718/151‑1 (MQ=255)
                                                                        tATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGC‑GACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGtt                                                                           >  1:4848069/1‑151 (MQ=255)
                                                                                   gCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGAAGA‑GACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCTAGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAgg                                                                >  2:588512/1‑151 (MQ=255)
                                                                                    gcTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGC‑GACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGGGGGAGGGCGCCGGCCGCGCCGAGGTGCAGGCCGAGGt                                                               >  1:4286368/2‑151 (MQ=255)
                                                                                     cTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGC‑GACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTg                                                              <  2:7604052/151‑1 (MQ=255)
                                                                                         aGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGC‑GACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTgg                                                          >  1:3621032/1‑151 (MQ=255)
                                                                                           ggcggcCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGC‑GACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGac                                                        >  1:11198563/1‑151 (MQ=255)
                                                                                            gcggcCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGC‑GACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGaca                                                       <  1:934926/151‑1 (MQ=255)
                                                                                                ccACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGC‑GACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCTGGCCGAGGTGCTGGACACCGc                                                   >  2:5974089/1‑151 (MQ=255)
                                                                                                 cACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGC‑GACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGcc                                                  >  2:1050497/1‑151 (MQ=255)
                                                                                                      ccgcGTCGACGTCGGCCTCGCGGGTGTAGC‑GACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCt                                             <  1:2772348/151‑1 (MQ=255)
                                                                                                       cgcgTCGACGTCGGCCTCGCGGGTGTAGC‑GACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTg                                            <  2:11328473/151‑1 (MQ=255)
                                                                                                       cgcgTCGACGTCGGCCTCGCGGGTGTAGC‑GACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTg                                            >  1:3397697/1‑151 (MQ=255)
                                                                                                         cgTCGACGTCGGCCTCGCGGGTGTAGC‑GACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGa                                          >  1:10524938/1‑151 (MQ=255)
                                                                                                           tCGACGGCGGCCTCGCGGGTGTAGC‑GACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGACGCACGTGGGACGGCGCCGGCCGCGCCGAGTGGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACt                                        >  2:4070645/1‑151 (MQ=255)
                                                                                                             gACGTCGGCCTCGCGGGTGTAGC‑GACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTcc                                      >  2:4898446/1‑151 (MQ=255)
                                                                                                              aCGTCGGCCTCGCGGGTGTAGC‑GACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCa                                     <  2:6402341/151‑1 (MQ=255)
                                                                                                                gTCGGCCTCGCGGGTGTAGC‑GACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGt                                   >  2:11134758/1‑151 (MQ=255)
                                                                                                                gTCGGCCTCGCGGGTGGAGC‑GACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGt                                   <  2:7428269/151‑1 (MQ=255)
                                                                                                                  cGGCCTCGCGGGTGTAGC‑GACCGAGGCTCAGGCGCACGCTGCGGCCCGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGGGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGc                                 >  1:644248/1‑151 (MQ=255)
                                                                                                                  cGGCCTCGCGGGGGTAGC‑GACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGc                                 <  1:9859283/151‑1 (MQ=255)
                                                                                                                    gCCTCGCGGGTGTAGC‑GACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCTCGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCt                               >  1:10107527/1‑151 (MQ=255)
                                                                                                                     ccTCGCGGGTGTAGC‑GACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTg                              <  1:2489139/151‑1 (MQ=255)
                                                                                                                     ccTCGCGGGTGTAGC‑GACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTg                              <  1:8285975/151‑1 (MQ=255)
                                                                                                                       tCGCGGGTGTAGC‑GACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTggg                            <  1:10250962/151‑1 (MQ=255)
                                                                                                                        cgcgGGTGTAGC‑GACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTgggg                           <  2:1173829/151‑1 (MQ=255)
                                                                                                                         gcgGGTGTAGC‑GACCGCGGCTCAGGCGCACGCTGCGGCCAGCGCTGTCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTggggg                          <  1:688414/151‑1 (MQ=255)
                                                                                                                         gcgGGTGTAGC‑GACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTggggg                          <  2:8569080/151‑1 (MQ=255)
                                                                                                                         gcgGGTGTAGC‑GACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTggggg                          <  1:3155550/151‑1 (MQ=255)
                                                                                                                          cgGGTGTAGC‑GACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTGGGGGa                         <  1:5198247/151‑1 (MQ=255)
                                                                                                                           gggTGTAGC‑GACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTGGGGGAc                        >  1:6580735/1‑151 (MQ=255)
                                                                                                                            ggTGTAGC‑GACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTGGGGGACa                       >  2:8262589/1‑151 (MQ=255)
                                                                                                                            ggTGTAGC‑GACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTGGGGGACa                       >  2:9224213/1‑151 (MQ=255)
                                                                                                                             gtgtAGC‑GACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTGGGGGACaa                      <  2:44648/151‑1 (MQ=255)
                                                                                                                             gtgtAGC‑GACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTGGGGGACaa                      <  1:2996829/151‑1 (MQ=255)
                                                                                                                               gtAGC‑GACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTGGGGGACAAAg                    >  1:8100855/1‑151 (MQ=255)
                                                                                                                               gtAGC‑GACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTGGGGGACAAAg                    <  2:4759890/151‑1 (MQ=255)
                                                                                                                                tAGC‑GACCGAGGCTCAGGCGCACGCTGCGGCCTGCGCTGGCGGCTTCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTGGGGGACAAAgg                   <  2:1912464/151‑1 (MQ=255)
                                                                                                                                 aGC‑GACCGTGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCGAGTGCCTGGGGGCCACAGGc                  >  1:1594079/1‑151 (MQ=255)
                                                                                                                                     gACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTGGGGGACAAAGGCCgg               <  1:7191905/151‑1 (MQ=255)
                                                                                                                                      aCCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTGGGGGACAAAGGCCggg              >  2:1415270/1‑151 (MQ=255)
                                                                                                                                      aCCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTGGGGGACAAAGGCCggg              <  2:62838/151‑1 (MQ=255)
                                                                                                                                        cGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTGGGGGACAAAGGCCGGGtt            <  2:5726672/151‑1 (MQ=255)
                                                                                                                                          aGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTGGGGGACAAAGGCCGGGTTGt          >  2:3552891/1‑151 (MQ=255)
                                                                                                                                          aGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTGGGGGACAAAGGCCGGGTTGt          <  1:10205818/151‑1 (MQ=255)
                                                                                                                                            gCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTGGGGGACAAAGGCCGGGTTGTCg        <  1:4151536/151‑1 (MQ=255)
                                                                                                                                             cTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTGGGGGACAAAGGCCGGGTTGTCGa       >  2:8897422/1‑151 (MQ=255)
                                                                                                                                                 ggCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTGGGGGACAAAGGCCGGGTTGTCGAAGGt   <  1:1695415/151‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
GAGGGCGGGTCGAGCCGGCCGCTCAGCCTGCCCTGGATACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGC‑GACCGAGGCTCAGGCGGACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTGGGGGACAAAGGCCGGGTTGTCGAAGGTC  >  NC_008463/3373519‑3373814

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑