Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 3,543,662 A→G T494A (ACG→GCG)  PA14_RS16110 → PucR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084633,543,6620AG100.0% 2213.6 / NA 555T494A (ACG→GCG) PA14_RS16110PucR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (249/306);  total (249/306)

Only 100 of 555 total aligned reads displayed.

GAACGCCGCGCAGCGGCTACACATACACAAGCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAACGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGACGCCGCCGCCAACAGGCGCGGCCAGGAACGGTTGCCTGGCCGCGG  >  NC_008463/3543514‑3543809
                                                                                                                                                    |                                                                                                                                                   
gAACGCCGCGCAGCGGCTACACATACACAAGCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCg                                                                                                                                                   <  1:646962/151‑1 (MQ=255)
gAACGCCGCGCAGCGGCTACACATACACAAGCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCg                                                                                                                                                   >  1:9055422/1‑151 (MQ=255)
 aaCGCCGCGCAGCGGCTACACATACACAAGCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGa                                                                                                                                                  >  2:371260/1‑151 (MQ=255)
   cgccgcGCAGCGGCTACACATACACAAGCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAgg                                                                                                                                                <  1:4902402/151‑1 (MQ=255)
    gccgcGCAGCGGCTACACATACACAAGCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAggg                                                                                                                                               >  1:11466595/1‑151 (MQ=255)
     ccgcGCAGCGGCTACACATACACAAGCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAgggg                                                                                                                                              <  2:2870130/151‑1 (MQ=255)
     ccgcGCAGCGGCTACACATACACAAGCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAgggg                                                                                                                                              >  1:2617878/1‑151 (MQ=255)
      cgcgCAGCGGCTACACATACACAAGCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGc                                                                                                                                             <  2:10315303/151‑1 (MQ=255)
        cgcAGCGGCTACACATACACAAGCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAg                                                                                                                                           >  1:3815111/1‑151 (MQ=255)
        cgcAGCGGCTACACATACACAAGCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAg                                                                                                                                           >  2:2486373/1‑151 (MQ=255)
         gcAGCGGCTACACATACACAAGCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAgg                                                                                                                                          >  1:990492/1‑151 (MQ=255)
         gcAGCGGCTACACATACACAAGCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAgg                                                                                                                                          <  2:3092182/151‑1 (MQ=255)
           aGCGGCTACACATACACAAGCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGAc                                                                                                                                        <  1:11335614/151‑1 (MQ=255)
           aGCGGCTACACATACACAAGCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGAc                                                                                                                                        <  2:4607394/151‑1 (MQ=255)
           aGCGGCTACACATACACAAGCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGAc                                                                                                                                        <  1:11082177/151‑1 (MQ=255)
            gCGGCTACACATACACAAGCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACGCGGCTGTCGCCGGAAGCGAGGGGCAGGACg                                                                                                                                       <  1:8506786/151‑1 (MQ=255)
             cGGCTACACATACACAAGCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCACCCCGGCTGTCGCCGGAAGCGAGGGGCAGGACgg                                                                                                                                      >  1:3196099/1‑151 (MQ=255)
              ggCTACACATACACAAGCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGc                                                                                                                                     >  1:9171186/1‑151 (MQ=255)
              ggCTACACATACACAAGCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGc                                                                                                                                     >  1:10240107/1‑151 (MQ=255)
                  acacatacacaAGCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAag                                                                                                                                 >  2:8546447/1‑151 (MQ=255)
                     catccacaAGCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCTCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGagc                                                                                                                              <  1:10087011/151‑1 (MQ=255)
                      atacacaAGCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGagca                                                                                                                             <  2:11539800/151‑1 (MQ=255)
                        acacaAGCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGagcagc                                                                                                                           <  1:1955826/151‑1 (MQ=255)
                         cacaAGCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGcc                                                                                                                          <  2:10563628/151‑1 (MQ=255)
                         cacaAGCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGcc                                                                                                                          <  2:5517365/151‑1 (MQ=255)
                          acaAGCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGcct                                                                                                                         >  1:10857349/1‑151 (MQ=255)
                           caAGCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGcctc                                                                                                                        >  2:2247018/1‑151 (MQ=255)
                           caAGCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGcctc                                                                                                                        <  2:8302546/151‑1 (MQ=255)
                            aaGCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGcctcc                                                                                                                       <  2:5269019/151‑1 (MQ=255)
                             aGCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGcctcct                                                                                                                      >  1:5521065/1‑151 (MQ=255)
                               cAGAGCCTGGTCTACCGCATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTgg                                                                                                                    <  2:10681169/151‑1 (MQ=255)
                                   gCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCa                                                                                                                <  1:7013353/151‑1 (MQ=255)
                                       ggTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTcc                                                                                                            <  2:6801710/151‑1 (MQ=255)
                                        gTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCcg                                                                                                           <  2:9618837/151‑1 (MQ=255)
                                         tCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCcgc                                                                                                          >  1:8114029/1‑151 (MQ=255)
                                          cTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCcgcg                                                                                                         >  2:1376009/1‑151 (MQ=255)
                                            aCCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAGGAGCAGCCTCCTGGAGCAGTCCGCGCa                                                                                                       >  1:3013509/1‑151 (MQ=255)
                                             ccGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCAc                                                                                                      <  2:10168508/151‑1 (MQ=255)
                                                  tACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATgg                                                                                                 >  1:7923155/1‑151 (MQ=255)
                                                        gcATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGTGCAGCCTCCGGGAGCATTCCGCGCACCAAGGCGGCGa                                                                                           >  2:1867967/1‑151 (MQ=255)
                                                             gAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATgg                                                                                      >  2:2040759/1‑151 (MQ=255)
                                                              aGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGGGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGa                                                                                     >  1:733422/1‑151 (MQ=255)
                                                                gAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACt                                                                                   <  1:6271922/151‑1 (MQ=255)
                                                                gAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACt                                                                                   >  2:10934303/1‑151 (MQ=255)
                                                                 aaaTCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTg                                                                                  >  1:2868651/1‑151 (MQ=255)
                                                                  aaTCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGa                                                                                 <  1:8980190/151‑1 (MQ=255)
                                                                  aaTCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGa                                                                                 <  1:2152742/151‑1 (MQ=255)
                                                                  aaTCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGa                                                                                 <  2:1117012/151‑1 (MQ=255)
                                                                    tCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGAcg                                                                               <  1:2786318/151‑1 (MQ=255)
                                                                    tCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGAcg                                                                               >  1:1714133/1‑151 (MQ=255)
                                                                     cAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGAcgg                                                                              <  1:8778687/151‑1 (MQ=255)
                                                                      tgCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCCTGGCGGCGACATGGACTGAcggc                                                                             >  1:8361584/2‑151 (MQ=255)
                                                                      aGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGAcggc                                                                             <  1:1056233/151‑1 (MQ=255)
                                                                       gCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGAcggcg                                                                            >  1:4027514/1‑151 (MQ=255)
                                                                         ggTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGa                                                                          >  2:6965065/1‑151 (MQ=255)
                                                                          gTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAg                                                                         <  1:3707598/151‑1 (MQ=255)
                                                                           tcgctcgcTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGc                                                                        <  2:2656230/151‑1 (MQ=255)
                                                                           tcgctcgcTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGc                                                                        >  2:10230245/1‑151 (MQ=255)
                                                                            cgctcgctGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGcc                                                                       <  2:650819/151‑1 (MQ=255)
                                                                             gctcgctGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGccc                                                                      <  2:10504538/151‑1 (MQ=255)
                                                                             gctcgctGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGccc                                                                      <  1:3948019/151‑1 (MQ=255)
                                                                             gctcgctGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGccc                                                                      <  1:2405604/151‑1 (MQ=255)
                                                                             gctcgctGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGccc                                                                      <  2:9422651/151‑1 (MQ=255)
                                                                             gctcgctGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGccc                                                                      <  1:964217/151‑1 (MQ=255)
                                                                               tcgctGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGa                                                                    >  1:9364828/1‑151 (MQ=255)
                                                                                cgctGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGAt                                                                   <  2:1205690/151‑1 (MQ=255)
                                                                                   tGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATAcc                                                                <  2:7551971/151‑1 (MQ=255)
                                                                                   tGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATAcc                                                                <  2:7761021/151‑1 (MQ=255)
                                                                                       cAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGgc                                                            <  2:4826448/151‑1 (MQ=255)
                                                                                        aGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGgcg                                                           >  1:6385939/1‑151 (MQ=255)
                                                                                          cACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCt                                                         >  2:3401483/1‑151 (MQ=255)
                                                                                          cACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCt                                                         <  1:768823/151‑1 (MQ=255)
                                                                                              gccgATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCa                                                     >  1:100171/1‑151 (MQ=255)
                                                                                                  aTGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGgac                                                 >  2:5580003/1‑151 (MQ=255)
                                                                                                   tgtgGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGgacg                                                >  2:6209884/1‑151 (MQ=255)
                                                                                                    gtgGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGgacga                                               >  2:5880541/1‑151 (MQ=255)
                                                                                                     tgGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGgacgac                                              <  1:7625495/151‑1 (MQ=255)
                                                                                                      ggCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGacgacg                                             >  1:11594761/1‑151 (MQ=255)
                                                                                                        cGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGAcgcc                                           <  1:4519672/151‑1 (MQ=255)
                                                                                                         gACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGAcgccg                                          <  1:1402472/151‑1 (MQ=255)
                                                                                                          tcccTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGAcgccgc                                         >  1:9753715/2‑151 (MQ=255)
                                                                                                           cccTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGAcgccgcc                                        <  2:9199729/151‑1 (MQ=255)
                                                                                                                   ttCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGACGCCGCCGCCAACAg                                <  2:316808/151‑1 (MQ=255)
                                                                                                                      gCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGACGCCGCCGCCAACAGgcg                             <  2:4169906/151‑1 (MQ=255)
                                                                                                                      gCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGACGCCGCCGCCAACAGgcg                             >  2:8642089/1‑151 (MQ=255)
                                                                                                                        cTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGACGCCGCCGCCAACAGGcgcg                           <  1:9233858/151‑1 (MQ=255)
                                                                                                                        cTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGACGCCGCCGCCAACAGGcgcg                           <  1:3518760/151‑1 (MQ=255)
                                                                                                                         ttGCAGGCAACCCGGCTGTCGCCGGACGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGACGCCGCCGCCAACAGGCGCgg                          >  2:8105765/1‑151 (MQ=255)
                                                                                                                         ttGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGACGCCGCCGCCAACAGGCGCgg                          >  2:3984454/1‑151 (MQ=255)
                                                                                                                             aGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGACGCCGCCGCCAACAGGCGCGGCCAg                      <  1:8671731/151‑1 (MQ=255)
                                                                                                                             aGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGACGCCGCCGCCAACAGGCGCGGCCAg                      >  1:5862458/1‑151 (MQ=255)
                                                                                                                                cAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGACGCCGCCGCCAACAGGCGCGGCCAGGaa                   <  1:4802557/151‑1 (MQ=255)
                                                                                                                                 gaCCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGTCGCCGCCGCCAACAGGCGCGGCCAGGagc                  >  1:6160521/2‑149 (MQ=255)
                                                                                                                                 aaCCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGACGCCGCCGCCAACAGGCGCGGCCAGGAAc                  >  2:1631189/1‑151 (MQ=255)
                                                                                                                                 aaCCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGACGCCGCCGCCAACAGGCGCGGCCAGGAAc                  <  2:4342516/151‑1 (MQ=255)
                                                                                                                                     cGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGACGCCGCCGCCAACAGGCGCGGCCAGGAACGGtt              >  1:52640/1‑151 (MQ=255)
                                                                                                                                       gCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGACGCCGCCGCCAACAGGCGCGGCCAGGAACGGTTGc            <  2:7099161/151‑1 (MQ=255)
                                                                                                                                            cGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGACGCCGCCGCCAACAGGCGCGGCCAGGAACGGTTGCCTGGc       >  2:11372453/1‑151 (MQ=255)
                                                                                                                                               cGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGACGCCGCCGCCAACAGGCGCGGCCAGGAACGGTTGCCTGGCcgc    <  1:4359431/151‑1 (MQ=255)
                                                                                                                                                ggAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGACGCCGCCGCCAACAGGCGCGGCCAGGAACGGTTGCCTGGCcgcg   >  2:7891103/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GAACGCCGCGCAGCGGCTACACATACACAAGCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAACGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGACGCCGCCGCCAACAGGCGCGGCCAGGAACGGTTGCCTGGCCGCGG  >  NC_008463/3543514‑3543809

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑