Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 4,707,658 G→C A165A (GCG→GCC)  PA14_RS21625 → zinc‑binding alcohol dehydrogenase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084634,707,6580GC99.9% 2667.7 / NA 669A165A (GCG→GCC) PA14_RS21625zinc‑binding alcohol dehydrogenase family protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base C (329/339);  minor base A (1/0);  total (330/339)
Fisher's exact test for biased strand distribution p-value = 4.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 669 total aligned reads displayed.

CCCAGGCCGCCAGTTGCGGGGTGCCCTACAGCACCGCCTGGGACGCCCTGCAACGCAGCCAGGTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCGGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCGGAGGTGATCTTCGATACG  >  NC_008463/4707510‑4707805
                                                                                                                                                    |                                                                                                                                                   
  cAGGCCGCCAGTTGCGGGGTGCCCTACAGCACCGCCTGGGACGCCCTGCAACGCAGCCAGGTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAg                                                                                                                                                 >  1:11011761/1‑151 (MQ=255)
   aGGCCGCCAGTTGCGGGGTGCCCTACAGCACCGCCTGGGACGCCCTGCAACGCAGCCAGGTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGc                                                                                                                                                >  1:5808582/1‑151 (MQ=255)
   aGGCCGCCAGTTGCGGGGTGCCCTACAGCACCGCCTGGGACGCCCTGCAACGCAGCCAGGTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGc                                                                                                                                                >  1:10502876/1‑151 (MQ=255)
   aGGCCGCCAGTTGCGGGGTGCCCTACAGCACCGCCTGGGACGCCCTGCAACGCAGCCAGGTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGc                                                                                                                                                >  1:2145171/1‑151 (MQ=255)
       cgccAGTTGCGGGGTGCCCTACAGCACCGCCTGGGACGCCCTGCAACGCAGCCAGGTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATc                                                                                                                                            >  1:4485484/1‑151 (MQ=255)
       cgccAGTTGCGGGGTGCCCTACAGCACCGCCTGGGACGCCCTGCAACGCAGCCAGGTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATc                                                                                                                                            <  1:8052954/151‑1 (MQ=255)
        gccAGTTGCGGGGTGCCCTACAGCACCGCCTGGGACGCCCTGCAACGCAGCCAGGTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCg                                                                                                                                           >  2:7607815/1‑151 (MQ=255)
          cAGTTGCGGGGTGCCCTACAGCACCGCCTGGGACGCCCTGCAACGCAGCCAGGTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGgc                                                                                                                                         <  2:8011730/151‑1 (MQ=255)
               gCGGGGTGCCCTACAGCACCGCCTGGGACGCCCTGCAACGCAGCCAGGTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGt                                                                                                                                    >  1:3030990/1‑151 (MQ=255)
                 ggggTGCCCTACAGCACCGCCTGGGACGCCCTGCAACGCAGCCAGGTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTgc                                                                                                                                  <  1:10692303/151‑1 (MQ=255)
                  gggTGCCCTACAGCACCGCCTGGGACGCCCTGCAACGCAGCCAGGTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTgcg                                                                                                                                 <  2:9483425/151‑1 (MQ=255)
                  gggTGCCCTACAGCACCGCCTGGGACGCCCTGCAACGCAGCCAGGTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTgcg                                                                                                                                 >  1:2333295/1‑151 (MQ=255)
                  gggTGCCCTACAGCACCGCCTGGGACGCCCTGCAACGCAGCCAGGTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTgcg                                                                                                                                 <  2:10993438/151‑1 (MQ=255)
                  gggTGCCCTACAGCACCGCCTGGGACGCCCTGCAACGCAGCCAGGTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCGGGCGATGGCCAAGGCCCGGGGGGCCGAAGCGATCTGCGCggagag                                                                                                                                 >  1:1641587/1‑147 (MQ=255)
                   ggTGCCCTACAGCACCGCCTGGGACGCCCTGCAACGCAGCCAGGTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTgcgg                                                                                                                                <  2:2603818/151‑1 (MQ=255)
                      gCCCTACAGCACCGCCTGGGACGCCCTGCAACGCAGCCAGGTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGgcgc                                                                                                                             >  2:10555870/1‑151 (MQ=255)
                        ccTACAGCACCGCCTGGGACGCCCTGCAACGCAGCCAGGTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCga                                                                                                                           >  2:9004902/1‑151 (MQ=255)
                         cTACAGCACCGCCTGGGACGCCCTGCAACGCAGCCAGGTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCgag                                                                                                                          >  1:1874825/1‑151 (MQ=255)
                         cTACAGCACCGCCTGGGACGCCCTGCAACGCAGCCAGGTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCgag                                                                                                                          >  1:8494393/1‑151 (MQ=255)
                          tACAGCACCGCCTGGGACGCCCTGCAACGCAGCCAGGTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCgagg                                                                                                                         <  1:6812819/151‑1 (MQ=255)
                           aCAGCACCGCCTGTGACGCCCTGCAACGCAGCCAGGTCAAGGCCGGCACCCGTCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCgagga                                                                                                                        <  2:3895905/151‑1 (MQ=255)
                           aCAGCACCGCCTGGGACGCCCTGCAACGCAGCCAGGTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCATGGCGGCGCTGGCGCTGGCCTAGGCCGTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCgagga                                                                                                                        <  1:5590549/151‑1 (MQ=255)
                           aCAGCACCGCCTGGGACGCCCTGCAACGCAGCCAGGTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCgagga                                                                                                                        <  2:9623631/151‑1 (MQ=255)
                                 ccGCCTGGGACGCCCTGCAACGCAGCCAGGTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACg                                                                                                                  <  2:11396658/151‑1 (MQ=255)
                                   gCCTGGGACGCCCTGCAACGCAGCCAGGTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGcc                                                                                                                <  2:3777821/151‑1 (MQ=255)
                                   gCCGGGGACGCCCTGCAACGCACCCAGGTGAATGGCGGCACCCCGCTCCGGGTAATCCGCGCCAACTGCGCGGTCGGCAAGGCCGCGCGGGCGCTGGCCGAGGCCCTGGGGGCCGCAGCGATCGGCGGCGTGCGGCGCGAGGAGCAACGcc                                                                                                                >  2:7820448/1‑151 (MQ=255)
                                    ccTGGGACGCCCTGCAACGCAGCCAGGTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCa                                                                                                               <  1:11377353/151‑1 (MQ=255)
                                      tGGGACGCCCTGCAACGCAGCCAGGTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAgg                                                                                                             >  1:1021959/1‑151 (MQ=255)
                                      tGGGACGCCCTGCAACGCAGCCAGGTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAgg                                                                                                             >  1:6666175/1‑151 (MQ=255)
                                          aCGCCCTGCAACGCAGCCAGGTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCt                                                                                                         <  2:6523543/151‑1 (MQ=255)
                                            gCCCTGCAACGCAGCCAGGTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTgg                                                                                                       >  2:2967588/1‑151 (MQ=255)
                                              ccTGCAACGCAGCCAGGTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGaa                                                                                                     <  2:3551246/151‑1 (MQ=255)
                                               cTGCAACGCAGCCAGGTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAg                                                                                                    >  1:4790115/1‑151 (MQ=255)
                                                     cGCAGCCAGGTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAgg                                                                                              <  2:169268/151‑1 (MQ=255)
                                                         gCCAGGTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCAt                                                                                          <  2:7487432/151‑1 (MQ=255)
                                                         gCCAGGTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCAt                                                                                          >  1:9980510/1‑151 (MQ=255)
                                                          ccAGGTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATc                                                                                         >  1:1410630/1‑151 (MQ=255)
                                                          ccAGGTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATc                                                                                         >  2:7909330/1‑151 (MQ=255)
                                                          ccAGGTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATc                                                                                         <  2:10733382/151‑1 (MQ=255)
                                                           cAGGTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATcg                                                                                        <  1:5313865/151‑1 (MQ=255)
                                                             ggTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATcgcc                                                                                      <  1:11284456/151‑1 (MQ=255)
                                                               tCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATcgccgc                                                                                    >  1:3922008/1‑151 (MQ=255)
                                                                  aGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCgct                                                                                 <  1:747510/151‑1 (MQ=255)
                                                                          aCCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTgg                                                                         >  1:8372605/1‑151 (MQ=255)
                                                                          aCCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTgg                                                                         >  1:9405704/1‑151 (MQ=255)
                                                                          aCCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTgg                                                                         <  2:185338/151‑1 (MQ=255)
                                                                           cccGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTggg                                                                        <  1:2577047/151‑1 (MQ=255)
                                                                           cccGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCGGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTggg                                                                        >  2:4616606/1‑151 (MQ=255)
                                                                              ggCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGACGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGa                                                                     >  1:10382047/1‑151 (MQ=255)
                                                                              ggCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGa                                                                     >  2:10726558/1‑151 (MQ=255)
                                                                              ggCGCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGa                                                                     >  1:7916544/1‑151 (MQ=255)
                                                                                cTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAgc                                                                   <  1:9101152/151‑1 (MQ=255)
                                                                                 tCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCACGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAgcc                                                                  >  2:8201676/1‑151 (MQ=255)
                                                                                 tCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAgcc                                                                  <  1:6398688/151‑1 (MQ=255)
                                                                                 tCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAgcc                                                                  >  2:7149565/1‑151 (MQ=255)
                                                                                  ccTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAgccg                                                                 <  2:5527411/151‑1 (MQ=255)
                                                                                  ccTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAgccg                                                                 <  2:1409168/151‑1 (MQ=255)
                                                                                   cTGGTAATCGGCGCCACCGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAgccgg                                                                <  1:724361/151‑1 (MQ=255)
                                                                                   cTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCACGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAgccgg                                                                <  2:5471573/151‑1 (MQ=255)
                                                                                   cTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAgccgg                                                                <  1:6186744/151‑1 (MQ=255)
                                                                                      gTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAgccggccg                                                             >  2:10165461/1‑151 (MQ=255)
                                                                                      gTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAgccggccg                                                             <  1:10065660/151‑1 (MQ=255)
                                                                                         aTCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCACGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAAc                                                          <  2:4033170/151‑1 (MQ=255)
                                                                                         aTCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAAc                                                          <  2:6158928/151‑1 (MQ=255)
                                                                                          tCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACt                                                         <  1:3649591/151‑1 (MQ=255)
                                                                                              cgcCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTggcg                                                     >  1:5009792/1‑151 (MQ=255)
                                                                                              cgcCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTggcg                                                     >  2:5874893/1‑151 (MQ=255)
                                                                                               gcCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCCTCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTggcgg                                                    >  2:830724/1‑151 (MQ=255)
                                                                                                 cAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGca                                                  <  1:2746665/151‑1 (MQ=255)
                                                                                                  aaCGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGcac                                                 <  2:9058527/151‑1 (MQ=255)
                                                                                                   aCGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGcaca                                                >  2:3220620/1‑151 (MQ=255)
                                                                                                   aCGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGcaca                                                >  2:6927768/1‑151 (MQ=255)
                                                                                                     ggCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAgg                                              <  2:6426924/151‑1 (MQ=255)
                                                                                                     ggCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAgg                                              <  1:4602299/151‑1 (MQ=255)
                                                                                                     ggCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAgg                                              <  2:5093913/151‑1 (MQ=255)
                                                                                                      gcgcGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGt                                             >  2:2415975/1‑151 (MQ=255)
                                                                                                      gcgcGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGt                                             <  2:4836724/151‑1 (MQ=255)
                                                                                                          ggTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGaa                                         >  2:3446334/1‑151 (MQ=255)
                                                                                                                cAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATc                                   <  1:1969959/151‑1 (MQ=255)
                                                                                                                   ggCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGCTCGGCGCGGTGCGGCGCGAGGAGCACCGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTc                                >  2:446219/1‑151 (MQ=255)
                                                                                                                     ccGCGCGGGCGCGGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTccc                              <  1:550677/151‑1 (MQ=255)
                                                                                                                      cgcgCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTcccc                             >  1:7665556/1‑151 (MQ=255)
                                                                                                                           tggcgctggcCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCcggcgg                        >  1:9744096/1‑151 (MQ=255)
                                                                                                                               gctggcCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCggcggggcc                    >  2:3732291/1‑150 (MQ=255)
                                                                                                                               gctggcCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCggcggggcc                    >  1:2977410/1‑150 (MQ=255)
                                                                                                                                 tggcCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGa                  >  2:8614572/1‑151 (MQ=255)
                                                                                                                                  ggcCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAg                 >  1:11515066/1‑151 (MQ=255)
                                                                                                                                   gcCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAgg                <  1:3298616/151‑1 (MQ=255)
                                                                                                                                   gcCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAgg                >  2:6027636/1‑151 (MQ=255)
                                                                                                                                     cAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCTGCGCGAGGAGCAACGCCAGGCGCTGGACGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTACCCCGGCGGGGCCGAGGTg              >  2:2127834/1‑151 (MQ=255)
                                                                                                                                       aGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGAt            <  2:11209179/151‑1 (MQ=255)
                                                                                                                                       aGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGAt            >  2:9266072/1‑151 (MQ=255)
                                                                                                                                       aGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCGGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGag            >  2:3282275/1‑150 (MQ=255)
                                                                                                                                        ggCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATc           >  1:6958634/1‑151 (MQ=255)
                                                                                                                                         gCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCt          <  2:4350675/151‑1 (MQ=255)
                                                                                                                                          cccTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCtt         <  1:10860474/151‑1 (MQ=255)
                                                                                                                                          cccTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCtt         >  1:3516843/1‑151 (MQ=255)
                                                                                                                                            cTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGTGGAGGAACGCCAGGCGCTGGAAGCGCAGGGCAGCGCCGGGCTGCTGCTGGGCGAGCCGGGCGGACTGGGGGCCGAGGTGGTAGGGATCTTCCCCGGCGGGGCCGAGGTGGTCTTCg       >  2:1255926/1‑151 (MQ=255)
                                                                                                                                                gggCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATAc   >  2:6713457/1‑151 (MQ=255)
                                                                                                                                                 ggCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACg  >  1:8847435/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CCCAGGCCGCCAGTTGCGGGGTGCCCTACAGCACCGCCTGGGACGCCCTGCAACGCAGCCAGGTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCGGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCGGAGGTGATCTTCGATACG  >  NC_008463/4707510‑4707805

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑