Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 5,028,199 G→C intergenic (‑121/+16) PA14_RS22975 ← / ← PA14_RS22980 Nramp family divalent metal transporter/ribbon‑helix‑helix domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084635,028,1990GC98.6% 1875.4 / 8.7 484intergenic (‑121/+16)PA14_RS22975/PA14_RS22980Nramp family divalent metal transporter/ribbon‑helix‑helix domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base G (3/4);  new base C (250/227);  total (253/231)
Fisher's exact test for biased strand distribution p-value = 7.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 484 total aligned reads displayed.

GCGTCGGCCGGCACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGGCCCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCCGGGTGGGTTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAAGGAGGCGAAGTTGAGCACCTCGCCGCGATAGTCCAT  >  NC_008463/5028059‑5028340
                                                                                                                                            |                                                                                                                                             
   tCGGCCGGCACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGGCCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCCGGTCGCGTCTTccctcccc                                                                                                                                >  2:4650680/1‑151 (MQ=255)
   tCGGCCGGCACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGGCCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCCGGTCGCGTCTTccctcccc                                                                                                                                <  1:357185/151‑1 (MQ=255)
   tCGGCCGGCACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGGCCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCCGGTCGCGTCTTccctcccc                                                                                                                                <  1:2318698/151‑1 (MQ=255)
   tCGGCCGGCACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGGCCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCCGGTCGCGTCTTccctcccc                                                                                                                                <  1:5695059/151‑1 (MQ=255)
   tCGGCCGGCACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGGCCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGACGGATCGGGGAGGACGTGCGACCAGCCGAAACGTTTCACCCCGGTCGCGTCTTccgtcccc                                                                                                                                >  2:4406848/1‑151 (MQ=255)
    cggccggcACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGGCCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCCGGTCGCGTCTTccctcccct                                                                                                                               <  2:8822313/151‑1 (MQ=255)
       ccggcACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGGCCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCCGGTCGCGTCTTCCCTCCCCTCAg                                                                                                                            >  1:829733/1‑151 (MQ=255)
        cggcACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGGCCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCCGGTCGCGTCTTCCCTCCCCTCAgg                                                                                                                           >  2:9587182/1‑151 (MQ=255)
            aCCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGGCCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCCGGTCGCGTCTTCCCTCCCCTCAGGCCTg                                                                                                                       >  1:4949279/1‑151 (MQ=255)
             ccGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGGCCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCCGGTCGCGTCTTCCCTCCCCTCAGGCCTgg                                                                                                                      <  1:6295862/151‑1 (MQ=255)
             ccGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGGCCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCCGGTCGCGTCTTCCCTCCCCTCAGGCCTgg                                                                                                                      <  1:1501093/151‑1 (MQ=255)
              cGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGGCCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCCGGTCGCGTCTTCCCTCCCCTCAGGCCTggg                                                                                                                     >  1:3940047/1‑151 (MQ=255)
                 cACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGGCCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCCGGTCGCGTCTTCCCTCCCCTCAGGCCTGGgcgc                                                                                                                  >  2:9709069/1‑151 (MQ=255)
                  aCGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGGCCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCCGGTCGCGTCTTCCCTCCCCTCAGGCCTGGgcgcg                                                                                                                 <  2:3154615/151‑1 (MQ=255)
                   cGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGGCCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCCGGTCGCGTCTTCCCTCCCCTCAGGCCTGGgcgcgc                                                                                                                >  2:6673751/1‑151 (MQ=255)
                    gCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGGCCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCCGGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCt                                                                                                               >  1:10284444/1‑151 (MQ=255)
                        tgGCTGACCGCCGAGGGACAGAACGGCGCGGTGGCCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCCGGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGcc                                                                                                           >  1:8648641/1‑151 (MQ=255)
                        tgGCTGACCGCCGAGGGACAGAACGGCGCGGTGGCCGTGGAGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCCGGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGcc                                                                                                           <  1:7942861/151‑1 (MQ=255)
                         ggCTGACCGCCGAGGGACAGAACGGCGCGGTGGCCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCCGGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCg                                                                                                          >  2:2133145/1‑151 (MQ=255)
                            tGACCGCCGAGGGACAGAACGGCGCGGTGGCCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCCGGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGAcc                                                                                                       >  1:244965/1‑151 (MQ=255)
                              aCCGCCGAGGGACAGAACGGCGCGGTGGCCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCCGGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAg                                                                                                     <  1:6593784/151‑1 (MQ=255)
                              aCCGCCGAGGGACAGAACGGCGCGGTGGCCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCCGGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAg                                                                                                     <  1:10041194/151‑1 (MQ=255)
                               ccgccgAGGGACAGAACGGCGCGGTGGCCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCCGGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAgg                                                                                                    >  1:10086136/1‑151 (MQ=255)
                               ccgccgAGGGACAGAACGGCGCGGTGGCCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCCGGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAgg                                                                                                    >  1:9101233/1‑151 (MQ=255)
                                  ccgAGGGACAGAACGGCGCGGTGGCCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCCGGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTc                                                                                                 <  2:4922307/151‑1 (MQ=255)
                                   cgAGGGACAGAACGGCGCGGTGGCCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCCGGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCa                                                                                                <  2:8902611/151‑1 (MQ=255)
                                    gAGGGACAGAACGGCGCGGTGGCCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCCGGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAg                                                                                               >  2:10863459/1‑151 (MQ=255)
                                    gAGGGACAGAACGGCGCGGTGGCCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCCGGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAg                                                                                               >  2:5525969/1‑151 (MQ=255)
                                    gAGGGACAGAACGGCGCGGTGGCCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCCGGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAg                                                                                               >  1:21979/1‑151 (MQ=255)
                                    gAGGGACAGAACGGCGCGGTGGCCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCCGGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAg                                                                                               >  2:3812779/1‑151 (MQ=255)
                                     aGGGACAGAACGGCGCGGTGGCCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCCGGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGc                                                                                              >  1:6839766/1‑151 (MQ=255)
                                       ggACAGAACGGCGCGGTGGCCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCCGGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTc                                                                                            <  2:9336794/151‑1 (MQ=255)
                                           aGACCGGCGCGGTGGCCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTACACGTTTCACCCCGGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCg                                                                                        <  1:4819517/151‑1 (MQ=255)
                                                 gcgcGGTGGCCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCCGGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTtc                                                                                  <  2:10107331/151‑1 (MQ=255)
                                                 gcgcGGTGGCCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCCGGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTtc                                                                                  <  1:11343467/151‑1 (MQ=255)
                                                    cgGTGGCCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCCGGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTcg                                                                               <  2:10725891/151‑1 (MQ=255)
                                                    cgGTGGCCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCCGGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTcg                                                                               <  1:6083855/151‑1 (MQ=255)
                                                            cGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCCGGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCCGGCTCAGCTCGGCGACCTTCTCGCGCCGCt                                                                        >  2:1052653/1‑151 (MQ=255)
                                                            cGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCCGGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCt                                                                        <  2:5300755/151‑1 (MQ=255)
                                                             gtggtgGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCCGGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTg                                                                       >  2:6082004/1‑151 (MQ=255)
                                                               ggtggGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCCGGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTgcg                                                                     >  2:7467769/1‑151 (MQ=255)
                                                                 tggGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCCGGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGcc                                                                   <  1:9867832/151‑1 (MQ=255)
                                                                     cAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCCGGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGt                                                               <  1:6731024/151‑1 (MQ=255)
                                                                        ggAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCCGGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGc                                                            >  2:7115553/1‑151 (MQ=255)
                                                                        ggAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCCGGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGc                                                            >  2:10444681/1‑151 (MQ=255)
                                                                            tATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCCGGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGt                                                        <  1:1868048/151‑1 (MQ=255)
                                                                              tttCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCCGGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGc                                                      >  2:3917672/1‑151 (MQ=255)
                                                                              tttCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCCGGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGc                                                      <  2:8342455/151‑1 (MQ=255)
                                                                                 cACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCCGGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAAc                                                   <  2:4558920/151‑1 (MQ=255)
                                                                                 cACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCCGGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAAc                                                   <  1:731148/151‑1 (MQ=255)
                                                                                 cACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCCGGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAAc                                                   <  1:5164569/151‑1 (MQ=255)
                                                                                   cGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCCGGTCGCGTCTTCCCTCCCCACAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGAGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTg                                                 >  1:11000176/1‑151 (MQ=255)
                                                                                    gAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCCGGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGa                                                <  2:4300026/151‑1 (MQ=255)
                                                                                     aGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCCGGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGac                                               <  2:7158177/151‑1 (MQ=255)
                                                                                         gACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCCGGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGc                                           >  1:8854595/1‑151 (MQ=255)
                                                                                         gACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCCGGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGc                                           >  2:5880707/1‑151 (MQ=255)
                                                                                         gACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCCGGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGc                                           >  2:7660815/1‑151 (MQ=255)
                                                                                          aCGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCCGGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCa                                          <  2:6965052/151‑1 (MQ=255)
                                                                                               ggATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCCGGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCGAGGTAGCGGGTGCAACTGACACGCcggaag                                     >  1:8488448/1‑151 (MQ=255)
                                                                                               ggATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCCGGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCACCTGACACGCaggaag                                     >  2:1768490/1‑151 (MQ=255)
                                                                                               ggATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCCGGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCaggagg                                     >  1:7037685/1‑149 (MQ=255)
                                                                                               ggATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCCGGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCaggaag                                     >  1:7790151/1‑151 (MQ=255)
                                                                                               ggATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCCGGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCaggaag                                     <  1:10615944/151‑1 (MQ=255)
                                                                                               ggATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCCGGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCaggaag                                     <  1:5288846/151‑1 (MQ=255)
                                                                                                gATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCCGGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCaggaagg                                    >  2:1528165/1‑151 (MQ=255)
                                                                                                gATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCCGGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCaggaagg                                    <  1:5399257/151‑1 (MQ=255)
                                                                                                gATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCCGGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCaggaagg                                    <  2:7291368/151‑1 (MQ=255)
                                                                                                 aTCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCCGGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCaggaagga                                   >  2:5191057/1‑151 (MQ=255)
                                                                                                   cGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCCGGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAaggagg                                 <  2:3379435/151‑1 (MQ=255)
                                                                                                    ggggAGGAAGTGCGACCAGCCTAAACGTTTCACCCCGGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAAGGAGGc                                >  2:914463/1‑151 (MQ=255)
                                                                                                    ggggAGGAAGTGCGACCAGCCTAAACGTTTCACCCCGGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAAGGAGGc                                <  1:6538535/151‑1 (MQ=255)
                                                                                                    ggggAGGAAGTGCGACCAGCCTAAACGTTTCACCCCGGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAAGGAGGc                                >  2:6529188/1‑151 (MQ=255)
                                                                                                     gggAGGAAGTGCGACCAGCCTAAACGTTTCACCCCGGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAAGGAGGCg                               <  2:6298803/151‑1 (MQ=255)
                                                                                                     gggAGGAAGTGCGACCAGCCTAAACGTTTCACCCCGGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAAGGAGGCg                               >  2:9875933/1‑151 (MQ=255)
                                                                                                      tgaggaAGTGCGACCAGCCTAAACGTGTCACCCCGGTCGCGTCTTACCTCACCTCAGTCCAGGGCGCGCTGGCCGACCAGGCTCAGCACGGCGACCTACTCGCGCCGCAGCGACAGGTAGCGGGTGCAACAGACACGCAGGAAGGAGGCGa                              >  2:5763126/2‑151 (MQ=255)
                                                                                                      ggaggaAGTGCGACCAGCCTAAACGTTTCACCCCGGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAAGGAGGCGa                              <  1:9396841/151‑1 (MQ=255)
                                                                                                       gaggaAGTGCGACCAGCCTAAACGTTTCACCCCGGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAAGGAGGCGaa                             <  1:3308230/151‑1 (MQ=255)
                                                                                                       gaggaAGTGCGACCAGCCTAAACGTTTCACCCCGGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAAGGAGGCGaa                             >  1:11576280/1‑151 (MQ=255)
                                                                                                          gaAGTGCGACCAGCCTAAACGTTTCACCCCGGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAAGGAGGCGAAGtt                          <  1:11463760/151‑1 (MQ=255)
                                                                                                            aGTGCGACCAGCCTAAACGTTTCACCCCGGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAAGGAGGCGAAGTTGa                        <  1:2594728/151‑1 (MQ=255)
                                                                                                            aGTGCGACCAGCCTAAACGTTTCACCCCGGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAAGGAGGCGAAGTTGa                        >  2:8016750/1‑151 (MQ=255)
                                                                                                                cGACCAGCCTAAACGTTTCACCCCGGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAAGGAGGCGAAGTTGAGCAc                    >  2:4550313/1‑151 (MQ=255)
                                                                                                                 gACCAGCCTAAACGTTTCACCCCGGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGTCCAGGCTCAGCTCGGCGACCTGCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAAGGAGGCGACGTTGAGCAcc                   >  1:439925/1‑151 (MQ=255)
                                                                                                                 gACCAGCCTAAACGTTTCACCCCGGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAAGGAGGCGAAGTTGAGCAcc                   >  1:4669446/1‑151 (MQ=255)
                                                                                                                 gACCAGCCTAAACGTTTCACCCCGGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCATCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAAGGAGGCGAAGTTGAGCAcc                   >  2:3661204/1‑151 (MQ=255)
                                                                                                                  aCCAGCCTAAACGTGTCACCCCGGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCGTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAAGGAGGCGAAGTTGAGCACCt                  <  2:1195342/151‑1 (MQ=255)
                                                                                                                   ccAGCCTAAACGTTTCACCCCGGTCGCGTCTTCCCTCCCCTCAGTCCTGGGCGAGCTGTCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAAGGAGGCGAAGTTGAGCACCTc                 <  2:11430993/151‑1 (MQ=255)
                                                                                                                     aGCCTAAACGTTTCACCCCGGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAAGGAGGCGAAGTTGAGCACCTcgc               <  1:1795670/151‑1 (MQ=255)
                                                                                                                     aGCCTAAACGTTTCACCCCGGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAAGGAGGCGAAGTTGAGCACCTcgc               >  2:6809690/1‑151 (MQ=255)
                                                                                                                      gCCTAAACGTTTCACCCCGGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAAGGAGGCGAAGTTGAGCACCTcgcc              <  2:10956331/151‑1 (MQ=255)
                                                                                                                      gCCTAAACGTTTCACCCCGGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAAGGAGGCGAAGTTGAGCACCTcgcc              <  1:6192331/151‑1 (MQ=255)
                                                                                                                       ccTAAACGTTTCACCCCGGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAAGGAGGCGAAGTTGAGCACCTcgccg             >  2:11380347/1‑151 (MQ=255)
                                                                                                                        cTAAACGTTTCACCCCGGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCTGGAAGGAGGCGAAGTTGAGCACCTcgccgc            >  2:8192721/1‑151 (MQ=255)
                                                                                                                         tAAACGTTTCACCCCGGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAAGGAGGCGAAGTTGAGCACCTCGCcgcg           <  2:8648641/151‑1 (MQ=255)
                                                                                                                             cGTTTCACCCCGGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCCGGTAGCGGGTGCAACTGACACGCAGGAAGGAGGCGAAGTTGAGCACCTCGCCGCGATAg       >  1:2930787/1‑151 (MQ=255)
                                                                                                                              gTTTCACCCCGGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAAGGAGGCGAAGTTGAGCACCTCGCCGCGATAGt      >  2:2417074/1‑151 (MQ=255)
                                                                                                                              gTTTCACCCCGGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAAGGAGGCGAAGTTGAGCACCTCGCCGCGATAGt      >  1:11098030/1‑151 (MQ=255)
                                                                                                                              gTTTCACCCCGGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCGCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGACGGAGGCGAAGTTGAGCACCTCGCCGCGATAGt      >  2:7733578/1‑151 (MQ=255)
                                                                                                                               tttCACCCCGGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAAGGAGGCGAAGTTGAGCACCTCGCCGCGATAGTc     >  1:7611438/1‑151 (MQ=255)
                                                                                                                                   caccccGGTGGCGTCTTCCCGCCCCTCAGGCCTGGGGGCGCGGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCACCTGTCACGCTGGAAGGAGGCGAAGTTGAGCTCCTCGCCGCGATCGTccct  >  2:11182976/3‑149 (MQ=255)
                                                                                                                                            |                                                                                                                                             
GCGTCGGCCGGCACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGGCCCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCCGGGTGGGTTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAAGGAGGCGAAGTTGAGCACCTCGCCGCGATAGTCCAT  >  NC_008463/5028059‑5028340

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: