BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_008463	5,809,318	G→C	intergenic (‑204/+15)	rpsF ← / ← rlmB	30S ribosomal protein S6/23S rRNA (guanosine(2251)‑2'‑O)‑methyltransferase RlmB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_008463	5,809,318	0	G	C	100.0%	1602.4 / NA	412	intergenic (‑204/+15)	rpsF/rlmB	30S ribosomal protein S6/23S rRNA (guanosine(2251)‑2'‑O)‑methyltransferase RlmB
Reads supporting (aligned to +/- strand): ref base G (0/0); new base C (231/181); total (231/181)

Only 100 of 412 total aligned reads displayed.

TTGGCTTGCCTGGTGGAGAGGCGAACGAACGCCTGCCCGCGCGGCAAGGGGCGACATTCTAGAGAAGGGCAACCGGCGAAGCAAGGAAGATTGGCGAATATTTGTACAGCGGATGGAATGGGCGCGCGGGGCCCCCCCCGGGGGGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTTGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAG  >  NC_008463/5809175‑5809461

ttcttggcttgcctggtggagaggcgaacgaacgcctgcccgcgcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGc                                                                                                                                         >  2:6239296‑M2/125‑151 (MQ=255)

ttcttggcttgcctggtggagaggcgaacgaacgcctgcccgcgcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGc                                                                                                                                         <  2:2037567‑M2/27‑1 (MQ=255)

 tcttggcttgcctggtggagaggcgaacgaacgcctgcccgcgcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacaccggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCa                                                                                                                                        <  1:9312573‑M2/28‑1 (MQ=255)

    tggcttgcctggtggagaggcgaacgaacgcctgcccgcgcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCAcgc                                                                                                                                     >  1:6662107‑M2/121‑151 (MQ=255)

      gcttgcctggtggagaggcgaacgaacgcctgcccgcgcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACgcgc                                                                                                                                   >  2:2051867‑M2/119‑151 (MQ=255)

       cttgccgggtggagaggcgaacgaacgcctgcccgcgcggcaaggggcgacattctagagacgggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCt                                                                                                                                  <  1:2296063‑M2/34‑1 (MQ=255)

         tgcctggtggagaggcgaacgaacgcctgcccgcgcggcaaggggcgacattctagagacgggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCa                                                                                                                                <  2:2643828‑M2/36‑1 (MQ=255)

         tgcctggtggagaggcgaacgaacgcctgcccgcgcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCa                                                                                                                                <  2:10998704‑M2/36‑1 (MQ=255)

         tgcctggtggagaggcgaacgaacgcctgcccgcgcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCCCACGCGCTCa                                                                                                                                >  1:8915320‑M2/116‑151 (MQ=255)

          gcctggtggagaggcgaacgaacgcctgcccgcgcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtaccgcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCAc                                                                                                                               <  1:7118735‑M2/37‑1 (MQ=255)

          gcctggtggagaggcgaacgaacgcctgcccgcgcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCAc                                                                                                                               <  1:1317413‑M2/37‑1 (MQ=255)

                  ccgcgcACGACCGCCAGCCCGCGCGGCACGGGGCGACATTCTCGACACGGGCAACCGGCGACGCAAGGAAGATGGGCGAAAAATTGCACAGCGGACGGAAT‑GGCGC‑CGGG‑‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGt                                                                                                                    <  2:6941259/149‑1 (MQ=255)

                     aggcgaacgaacgcctgcccgcgcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGt                                                                                                                    >  1:326423‑M2/104‑151 (MQ=255)

                      ggcgaacgaacgcctgcccgcgcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTg                                                                                                                   <  1:495111‑M2/49‑1 (MQ=255)

                         gaacgaacgcctgcccgcgcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggagggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTgcgc                                                                                                                >  2:9420185‑M2/100‑151 (MQ=255)

                          aacgaacgcctgcccgcgcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggacgattggcgaatagttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCCCGCGCGCACTCCAGCGGGGTGCGCt                                                                                                               >  1:10044546‑M2/99‑151 (MQ=255)

                             gaacgcctgcccgcgcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaagggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGc                                                                                                            <  2:1582600‑M2/56‑1 (MQ=255)

                              aacgcctgcccgcgcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCg                                                                                                           >  1:10878793‑M2/95‑151 (MQ=255)

                                cgcctgcccgcgcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCgga                                                                                                         >  2:3832463‑M2/93‑151 (MQ=255)

                                 gcctgcccgcgcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaagggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCggag                                                                                                        <  1:8635312‑M2/60‑1 (MQ=255)

                                 gccggcccgcgcggcacggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtccagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCggag                                                                                                        <  2:3664901‑M2/60‑1 (MQ=255)

                                   ctgcccgcgcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagaatcgcgactatatgtacagcagatggaatggCGCGGGG‑ACGCGCCGCGGCGGCCCCGCACGAGCTCACTCCAGCGGGGTGCGCAggaggagga                                                                                                      >  2:8070587‑M2/90‑151 (MQ=255)

                                    tgcccgcgcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGAt                                                                                                     <  1:10414322‑M2/63‑1 (MQ=255)

                                     gcccgcgcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtccagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATc                                                                                                    <  1:1228440‑M2/64‑1 (MQ=255)

                                      cccgcgcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCt                                                                                                   <  2:6366381‑M2/65‑1 (MQ=255)

                                       ccgcgcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGTGGATctc                                                                                                  >  1:6917491‑M2/86‑150 (MQ=255)

                                       ccgcgcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAggatgtc                                                                                                  >  1:3756324‑M2/86‑148 (MQ=255)

                                       ccgcgcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATctc                                                                                                  >  1:4359707‑M2/86‑150 (MQ=255)

                                            cggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACa                                                                                             >  2:11153515‑M2/81‑151 (MQ=255)

                                              gcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAgg                                                                                           <  2:2297645‑M2/73‑1 (MQ=255)

                                               caaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGc                                                                                          >  2:1042838‑M2/78‑151 (MQ=255)

                                               caaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGc                                                                                          >  2:7476494‑M2/78‑151 (MQ=255)

                                               caaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCGGGCGGAGGATCTCGAACAGGc                                                                                          >  1:7970987‑M2/78‑151 (MQ=255)

                                                 aggggcgcaattctatgcgagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAg                                                                                        <  1:7586977‑M2/76‑1 (MQ=255)

                                                  ggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATGTCGTACAGGCAGa                                                                                       >  1:3176856‑M2/75‑151 (MQ=255)

                                                  ggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGa                                                                                       >  2:5442359‑M2/75‑151 (MQ=255)

                                                   gggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGAc                                                                                      <  1:4186407‑M2/78‑1 (MQ=255)

                                                      cgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACACCAGCGGGGTGCGCTGGCGGAGGATCACGAACAGGCAGACGcc                                                                                   >  1:2944627‑M2/71‑151 (MQ=255)

                                                       gacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGAAGCCg                                                                                  >  1:390588‑M2/70‑151 (MQ=255)

                                                        acattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCgg                                                                                 >  1:5304465‑M2/69‑151 (MQ=255)

                                                         cattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGt                                                                                >  1:6396890‑M2/68‑151 (MQ=255)

                                                           ttctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCGCGAACAGGCAGACGCCGGTgg                                                                              >  2:7115685‑M2/66‑151 (MQ=255)

                                                             ctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCg                                                                            <  1:11063228‑M2/88‑1 (MQ=255)

                                                             ctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCACGAACAGGCAGACGCCGGTGGCg                                                                            <  2:6768792‑M2/88‑1 (MQ=255)

                                                              tagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCGCACTCCAGCGGGGTGCGCTGGCGGAGGTTCTCGAACAGGCAGACGCCGGTGGCGa                                                                           >  1:6104419‑M2/63‑151 (MQ=255)

                                                               agagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGAc                                                                          >  2:1121284‑M2/62‑151 (MQ=255)

                                                               agagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGAc                                                                          >  1:1692293‑M2/62‑151 (MQ=255)

                                                               agagaagggcaaccggcgaagcaaggaagattggcgaatattagtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGAc                                                                          >  2:4967045‑M2/62‑151 (MQ=255)

                                                                 agaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCg                                                                        <  2:3286943‑M2/92‑1 (MQ=255)

                                                                   aagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCgag                                                                      >  2:1010014‑M2/58‑151 (MQ=255)

                                                                       gcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGt                                                                  <  2:7711888‑M2/98‑1 (MQ=255)

                                                                         aaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGCTCTCGAACAGGCAGACGCCGGTGGCGACCGAGAcgtgc                                                                >  1:236963‑M2/52‑149 (MQ=255)

                                                                         aaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGTACAGGCAGACGCCGGTGGCGACCGAGACGTTc                                                                >  1:450772‑M2/52‑151 (MQ=255)

                                                                         aaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTc                                                                >  2:7550184‑M2/52‑151 (MQ=255)

                                                                              gcgacgcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGgct                                                           <  2:5909955‑M2/105‑1 (MQ=255)

                                                                              gcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCAGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGgct                                                           <  1:1504351‑M2/105‑1 (MQ=255)

                                                                                gaagcaaggaagattggcgaatatttgtacagcggatggactggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGgctgc                                                         <  2:4435563‑M2/107‑1 (MQ=255)

                                                                                 aagcacggaagattggcgaatatttgtaccgcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGgctgct                                                        <  1:11531537‑M2/108‑1 (MQ=255)

                                                                                 aagcaaggaagattggcgaatatttgtacagcggatggcatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCCGCGGGGTGCGCTGGCGGAGGGTCTCGAACAGGCAGACGCCGGTGGCGACCGCGACGTTCAGgctgct                                                        >  2:10687979‑M2/44‑151 (MQ=255)

                                                                                 aagcaaggaagattggcgaatatgtgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGgctgct                                                        <  2:7298416‑M2/108‑1 (MQ=255)

                                                                                   gcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGa                                                      >  2:3219340‑M2/42‑151 (MQ=255)

                                                                                    caaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTACAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGGTCAGGCTGCTGAc                                                     >  1:3339518‑M2/41‑151 (MQ=255)

                                                                                      aggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGc                                                   >  2:976574‑M2/39‑151 (MQ=255)

                                                                                        gaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTg                                                 <  1:5469323‑M2/115‑1 (MQ=255)

                                                                                          agattggcgaatatttgtcccgcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGcc                                               <  1:5587016‑M2/117‑1 (MQ=255)

                                                                                          agattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACACCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGcc                                               >  1:7910772‑M2/35‑151 (MQ=255)

                                                                                          agattggcgaatattggtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCACGAACAGGCAGACGCCGGTGGCGACCTAGACGTTCAGGCTGCTGACGCTGcc                                               <  2:1518323‑M2/117‑1 (MQ=255)

                                                                                           gattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCg                                              <  1:2785511‑M2/118‑1 (MQ=255)

                                                                                            attggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCACACTCCAGCGGGGTGCGCTGGCGGCGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCgg                                             >  2:130581‑M2/33‑151 (MQ=255)

                                                                                             ttggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGc                                            <  1:10533357‑M2/120‑1 (MQ=255)

                                                                                              tggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGcc                                           <  2:9115771‑M2/121‑1 (MQ=255)

                                                                                              tggcgaatatttgtacagcggagggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAAAAGGCAGTCGCCGGTGGCGACCGAGACGGTCAGGCTGCTGACGCTGCCGGcc                                           >  1:5751370‑M2/31‑151 (MQ=255)

                                                                                                  gaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACCGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCg                                       >  1:9430324‑M2/27‑151 (MQ=255)

                                                                                                   aatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCgg                                      <  2:10198094‑M2/126‑1 (MQ=255)

                                                                                                    atatttgtccagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGc                                     <  1:7422626‑M2/127‑1 (MQ=255)

                                                                                                            acagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCCCGCGCTCACTCCAGCGGGGTGCCCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGGTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGc                             >  2:5154424‑M2/17‑151 (MQ=255)

                                                                                                              agcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGa                           <  2:4362303‑M2/137‑1 (MQ=255)

                                                                                                               gcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAg                          <  1:1968913‑M2/138‑1 (MQ=255)

                                                                                                                cggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAgg                         >  1:924841‑M2/13‑151 (MQ=255)

                                                                                                                  gatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCCCTCCAGCGGGGTGCGCAGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTTGCGAGGaa                       >  1:2511364‑M2/11‑151 (MQ=255)

                                                                                                                  gatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGaa                       >  1:8426441‑M2/11‑151 (MQ=255)

                                                                                                                    tggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGt                     >  2:3531136‑M2/9‑151 (MQ=255)

                                                                                                                    tggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGt                     <  2:9360161‑M2/143‑1 (MQ=255)

                                                                                                                        atggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCa                 >  2:5750683‑M2/5‑151 (MQ=255)

                                                                                                                        atggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCa                 >  2:2160626‑M2/5‑151 (MQ=255)

                                                                                                                           gCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTg              >  2:8583302‑M2/2‑151 (MQ=255)

                                                                                                                             gcgGGGCCGCG‑CCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCACCCTGGCGAGGACGTCGCAGTGCt            >  2:3964372/1‑151 (MQ=255)

                                                                                                                             gcgGGGCCGCG‑CCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCt            >  1:9576488/1‑151 (MQ=255)

                                                                                                                             gcgGGGCCGCG‑CCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCt            >  2:9524049/1‑151 (MQ=255)

                                                                                                                             gcgGGGCCGCG‑CCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCt            <  1:9043291/151‑1 (MQ=255)

                                                                                                                             gcgGGGCCGCG‑CCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCt            >  1:5851423/1‑151 (MQ=255)

                                                                                                                             gcgGGGCCGCG‑CCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCt            >  1:466850/1‑151 (MQ=255)

                                                                                                                                  ggccgcgccGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTcgcg        <  2:8852427/144‑1 (MQ=255)

                                                                                                                                  ggccgcgccGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTcgcg        >  2:7071804/8‑151 (MQ=255)

                                                                                                                                     cgcgccGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGt     >  1:11388318/5‑151 (MQ=255)

                                                                                                                                     cgcgccGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGt     <  2:1883113/147‑1 (MQ=255)

                                                                                                                                     cgcgccGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGt     >  1:3558456/5‑151 (MQ=255)

                                                                                                                                      gcgccGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTc    <  2:8972727/148‑1 (MQ=255)

                                                                                                                                       cgccGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCa   >  2:4762581/3‑151 (MQ=255)

                                                                                                                                        gccGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAg  >  2:1186701/2‑151 (MQ=255)

TTGGCTTGCCTGGTGGAGAGGCGAACGAACGCCTGCCCGCGCGGCAAGGGGCGACATTCTAGAGAAGGGCAACCGGCGAAGCAAGGAAGATTGGCGAATATTTGTACAGCGGATGGAATGGGCGCGCGGGGCCCCCCCCGGGGGGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTTGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAG  >  NC_008463/5809175‑5809461

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`