Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 5,866,730 T→G A733A (GCA→GCC)  PA14_RS26890 ← ATP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084635,866,7300TG99.8% 2287.1 / NA 579A733A (GCA→GCC) PA14_RS26890ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base G (361/217);  minor base C (1/0);  total (362/217)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 579 total aligned reads displayed.

CGGATGAGCGGATTATTGGCGGCGGATGTAAGACGCCGTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCTGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGCCACCAGGCCCATGGAGTGGCAGCTTCCGGACAGGCGATGG  >  NC_008463/5866582‑5866877
                                                                                                                                                    |                                                                                                                                                   
cGGATGAGCGGATTATTGGCGGCGGATGTAAGACGCCGTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGgc                                                                                                                                                   <  1:11538014/151‑1 (MQ=255)
 ggATGAGCGGATTATTGGCGGCGGATGTAAGACGCCGTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGgcg                                                                                                                                                  >  2:10198661/1‑151 (MQ=255)
    tGAGCGGATTATTGGCGGCGGATGTAAGACGCCGTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCg                                                                                                                                               >  2:7499895/1‑151 (MQ=255)
          gATTATTGGCGGCGGATGTAAGACGCCGTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCgcgg                                                                                                                                         >  1:6139063/1‑151 (MQ=255)
           attattGGCGGCGGATGTAAGACGCCGTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCgcggc                                                                                                                                        >  1:10879326/1‑151 (MQ=255)
            ttattGGCGGCGGATGTAAGACGCCGTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCgcggcg                                                                                                                                       >  1:4474926/1‑151 (MQ=255)
                    ggcggATGTAAGACGCCGTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTAc                                                                                                                               >  1:3312931/1‑151 (MQ=255)
                     gcggATGTAAGACGCCGTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGACGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTAcc                                                                                                                              >  2:2117705/1‑151 (MQ=255)
                       ggATGTAAGACGCCGTTGCACGCGCGGATCGAAGATGTAAACAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCa                                                                                                                            <  1:2148752/151‑1 (MQ=255)
                         aTGTAAGACGCCGTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAgg                                                                                                                          <  2:5183280/151‑1 (MQ=255)
                            tAAGACGCCGTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGt                                                                                                                       >  1:7674329/1‑151 (MQ=255)
                            tAAGACGCCGTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGt                                                                                                                       >  1:9055614/1‑151 (MQ=255)
                             aaGACGCCGTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTg                                                                                                                      >  1:9641564/1‑151 (MQ=255)
                              aGACGCCGTTGACCGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTgc                                                                                                                     <  1:1281183/151‑1 (MQ=255)
                                  gCCGTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGccc                                                                                                                 >  1:5909706/1‑151 (MQ=255)
                                   ccGTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGcccc                                                                                                                >  1:7875217/1‑151 (MQ=255)
                                   ccGTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGcccc                                                                                                                <  1:11151888/151‑1 (MQ=255)
                                    cGTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCa                                                                                                               >  1:7215137/1‑151 (MQ=255)
                                     gTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTCCCCAGGCGTGCGCCCCAg                                                                                                              >  2:8750771/1‑151 (MQ=255)
                                     gTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAg                                                                                                              >  2:5721221/1‑151 (MQ=255)
                                     gTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAg                                                                                                              >  1:4020801/1‑151 (MQ=255)
                                          aCGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTcg                                                                                                         <  1:5466744/151‑1 (MQ=255)
                                            gcgcgGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTcgcg                                                                                                       <  1:7452737/151‑1 (MQ=255)
                                            gcgcgGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTcgcg                                                                                                       <  1:11293862/151‑1 (MQ=255)
                                              gcgGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCggg                                                                                                     <  2:3515530/151‑1 (MQ=255)
                                              gcgGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCggg                                                                                                     >  1:2370609/1‑151 (MQ=255)
                                               cgGATCGACGATGTAAAAAGCGGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGGCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGt                                                                                                    >  2:4576107/1‑151 (MQ=255)
                                               cgGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGt                                                                                                    <  2:6398547/151‑1 (MQ=255)
                                               cgGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGt                                                                                                    <  1:8583806/151‑1 (MQ=255)
                                                 gATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCg                                                                                                  >  2:1401405/1‑151 (MQ=255)
                                                 gATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCg                                                                                                  >  1:11065508/1‑151 (MQ=255)
                                                 gATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCg                                                                                                  >  2:8325968/1‑151 (MQ=255)
                                                  aTCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGa                                                                                                 >  1:5690851/1‑151 (MQ=255)
                                                  aTCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGa                                                                                                 >  1:6589458/1‑151 (MQ=255)
                                                   tCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAAGCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGAc                                                                                                >  1:10735854/1‑151 (MQ=255)
                                                    cGAAGATGTACAACGCTGTAAGGAAGGTGTTCGCCGCGCCGCCCAACCGGCCGCTGCGTCGGCTTCGCCGAGGACGCCGGCGCGGCGCAGGGCGTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGAcc                                                                                               <  2:7256688/151‑1 (MQ=255)
                                                    cGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGAcc                                                                                               >  2:4774777/1‑151 (MQ=255)
                                                    cGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGAcc                                                                                               >  1:6800575/1‑151 (MQ=255)
                                                     gAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGAccc                                                                                              >  2:5404731/1‑151 (MQ=255)
                                                      aaGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGAcccc                                                                                             <  2:2583883/151‑1 (MQ=255)
                                                      aaGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGAcccc                                                                                             >  1:518484/1‑151 (MQ=255)
                                                          tGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGcc                                                                                         <  2:11460256/151‑1 (MQ=255)
                                                          tGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGcc                                                                                         <  2:11019571/151‑1 (MQ=255)
                                                          tGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGcc                                                                                         >  2:4979776/1‑151 (MQ=255)
                                                          tGTAAAAAGCTGGAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGcc                                                                                         <  1:9322091/151‑1 (MQ=255)
                                                               aaaGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGcc                                                                                    >  2:5578240/1‑151 (MQ=255)
                                                                aaGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGccc                                                                                   <  1:4841438/151‑1 (MQ=255)
                                                                 tgCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCa                                                                                  <  1:3486068/150‑1 (MQ=255)
                                                                 aGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCa                                                                                  <  1:7530805/151‑1 (MQ=255)
                                                                  gCTGTACGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGccccg                                                                                 <  2:3713040/151‑3 (MQ=255)
                                                                  gCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAg                                                                                 >  2:8636844/1‑151 (MQ=255)
                                                                   cTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAgg                                                                                >  2:4021302/1‑151 (MQ=255)
                                                                   cTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAgg                                                                                >  2:11355305/1‑151 (MQ=255)
                                                                    tGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAggg                                                                               >  1:2962568/1‑151 (MQ=255)
                                                                    tGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAggg                                                                               >  2:3188040/1‑151 (MQ=255)
                                                                      tAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGcc                                                                             >  1:1794957/1‑151 (MQ=255)
                                                                         ggAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTcg                                                                          <  1:371662/151‑1 (MQ=255)
                                                                         ggAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTcg                                                                          >  1:10462070/1‑151 (MQ=255)
                                                                          gAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGACGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCGTcgc                                                                         >  1:8501074/1‑151 (MQ=255)
                                                                           aaGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCTGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCCGGTCGACCCCGGCCGCGCCCAGGGCCTcgcg                                                                        >  2:61696/1‑151 (MQ=255)
                                                                           aaGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTcgcg                                                                        >  2:10425756/1‑151 (MQ=255)
                                                                           aaGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTcgcg                                                                        <  1:5363757/151‑1 (MQ=255)
                                                                              ttgttCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTc                                                                     >  1:8206946/1‑151 (MQ=255)
                                                                              ttgttCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTc                                                                     >  2:6676108/1‑151 (MQ=255)
                                                                               tgttCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTcc                                                                    >  2:4257660/1‑151 (MQ=255)
                                                                               tgttCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTcc                                                                    >  2:9801747/1‑151 (MQ=255)
                                                                                 ttCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAg                                                                  >  2:8799758/1‑151 (MQ=255)
                                                                                 ttCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAg                                                                  <  1:7139664/151‑1 (MQ=255)
                                                                                   cgccgcgccgCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGTCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCt                                                                <  1:2758494/151‑1 (MQ=255)
                                                                                   cgccgcgccgCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCt                                                                >  1:7922105/1‑151 (MQ=255)
                                                                                    gccgcgccgcTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCTCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTc                                                               >  1:3086392/1‑151 (MQ=255)
                                                                                         gccgcTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCa                                                          >  2:3836430/1‑151 (MQ=255)
                                                                                          ccgcTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAg                                                         >  2:9300300/1‑151 (MQ=255)
                                                                                           cgcTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAgg                                                        >  2:2320539/1‑151 (MQ=255)
                                                                                           cgcTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAgg                                                        >  1:10062364/1‑151 (MQ=255)
                                                                                             cTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGcc                                                      <  1:4136913/151‑1 (MQ=255)
                                                                                              tCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCg                                                     >  2:5524924/1‑151 (MQ=255)
                                                                                                 aCCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGgcg                                                  <  2:2980895/151‑1 (MQ=255)
                                                                                                    ggCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCg                                               <  1:11459525/151‑1 (MQ=255)
                                                                                                        gCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAgcg                                           >  1:10507581/1‑151 (MQ=255)
                                                                                                              tCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGccacca                                     >  2:10683383/1‑151 (MQ=255)
                                                                                                                ggCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGCCACCAgg                                   >  2:2791885/1‑151 (MQ=255)
                                                                                                                ggCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGCCACCAgg                                   >  1:158054/1‑151 (MQ=255)
                                                                                                                   ttCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGCCACCAGGccc                                >  1:6283134/1‑151 (MQ=255)
                                                                                                                     cGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGCCACCAGGCCCAt                              >  2:10826574/1‑151 (MQ=255)
                                                                                                                     cGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGCCACCAGGCCCAt                              <  1:4695332/151‑1 (MQ=255)
                                                                                                                      gCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGCCACCAGGCCCATg                             <  2:8114140/151‑1 (MQ=255)
                                                                                                                       ccGAGGAAGCCGGCGCGGCGGAGGGGTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGCCACCTGGCCCATgg                            <  2:6886428/151‑1 (MQ=255)
                                                                                                                                ccGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGCCACCAGGCCCATGGAGTGGCAGc                   >  1:9884753/1‑151 (MQ=255)
                                                                                                                                  ggcgcggcgcAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCGCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGCCACCAGGCCCATGGAGTGGCAGCtg                 >  2:1974441/1‑150 (MQ=255)
                                                                                                                                     gcggcgcAGGGCTTCGGCGCCGTCGCGGCTCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGCCACCAGGCCCATGGAGTGGCAGCTTCCg              <  2:11577145/151‑1 (MQ=255)
                                                                                                                                     gcggcgcAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGCCACCAGGCCCATGGAGTGGCAGCTTCCg              <  2:11124884/151‑1 (MQ=255)
                                                                                                                                          gcAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGCCACCAGGCCCATGGAGTGGCAGCTTCCGGACAg         <  1:8371185/151‑1 (MQ=255)
                                                                                                                                          gcAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGCCACCAGGCCCATGGAGTGGCAGCTTCCGGACAg         <  1:8754660/151‑1 (MQ=255)
                                                                                                                                          gcAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGCCACCAGGCCCATGGAGTGGCAGCTTCCGGACAg         >  1:10193393/1‑151 (MQ=255)
                                                                                                                                           cAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGCCACCAGGCCCATGGAGTGGCAGCTTCCGGACAgg        <  2:6093304/151‑1 (MQ=255)
                                                                                                                                           cAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGCCACCAGGCCCATGGAGTGGCAGCTTCCGGACAgg        >  1:6090346/1‑151 (MQ=255)
                                                                                                                                            aGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGCCACCAGGCCCATGGAGTGGCAGCTTCCGGACAggc       <  2:7712119/151‑1 (MQ=255)
                                                                                                                                             gggCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGCCACCAGGCCCATGGAGTGGCAGCTTCCGGACAggcg      <  1:6500147/151‑1 (MQ=255)
                                                                                                                                              ggCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGCCACCAGGCCCATGGAGTGGCAGCTTCCGGACAggcga     <  1:9942988/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CGGATGAGCGGATTATTGGCGGCGGATGTAAGACGCCGTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCTGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGCCACCAGGCCCATGGAGTGGCAGCTTCCGGACAGGCGATGG  >  NC_008463/5866582‑5866877

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑