Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 729,520 C→T W66* (TGG→TAG)  PA14_RS03435 ← anhydro‑N‑acetylmuramic acid kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_008463729,5200CT99.8% 2226.8 / NA 522W66* (TGG→TAG) PA14_RS03435anhydro‑N‑acetylmuramic acid kinase
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base T (248/273);  total (248/274)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 522 total aligned reads displayed.

CAGGGCGGGGTTGCCGATCTGCACCGTGAAGTGGCGAGTGGGTTCATGTCGGATGGTTTGACCGTGACTGCCGATAGCACGGACTTCGTCGGGGCTCATCTGCTGCTGGAGCAGCAGTTCGCGCACGCCCTGTGCTGCAAGGGCGACCCAGCGTTGCTCGACTTCGGCGGCGCGGGCGATTTCATCGGGACCGGGGACGCACAGGGCGAGGATGTCTTCGCGCAGCCCGGCCGGCATGGGTAGGTAATGGGAAGCGAGCAAGGTGGTGCGATCACCTTGCTCGATCAGGACGATGT  >  NC_008463/729372‑729667
                                                                                                                                                    |                                                                                                                                                   
 agGGCGGGGTTGCCGATCTGCACCGTGAAGTGGCGAGTGGGTTCATGTCGGATGGTTTGACCGTGACTGCCGATAGCACGGACTTCGTCGGGGCTCATCTGCTGCTGGAGCAGCAGTTCGCGCACGCCCTGTGCTGCAAGGGCGACCTAGc                                                                                                                                                  <  1:9601556/151‑1 (MQ=255)
  gggCGGGGTTGCCGATCTGCACCGTGAAGTGGCGAGTGGGTTCATGTCGGATGGTTTGACCGTGACTGCCGATAGCACGGACTTCGTCGGGGCTCATCTGCTGCTGGAGCAGCAGTTCGCGCACGCCCTGTGCTGCAAGGGCGACCTAGCg                                                                                                                                                 <  2:688058/151‑1 (MQ=255)
  gggCGGGGTTGCCGATCTGCACCGTGAAGTGGCGAGTGGGTTCATGTCGGATGGTTTGACCGTGACTGCCGATAGCACGGACTTCGTCGGGGCTCATCTGCTGCTGGAGCAGCAGTTCGCGCACGCCCTGTGCTGCAAGGGCGACCTAGCg                                                                                                                                                 <  1:3579653/151‑1 (MQ=255)
   ggCGGGGTTGCCGATCTGCACCGTGAAGTGGCGAGTGGGTTCATGTCGGATGGTTTGACCGTGACTGCCGATAGCACGGACTTCGTCGGGGCTCATCTGCTGCTGGAGCAGCAGTTCGCGCACGCCCTGTGCTGCAAGGGCGACCTAGCGt                                                                                                                                                <  1:10008566/151‑1 (MQ=255)
   ggCGGGGTTGCCGATCTGCACCGTGAAGTGGCGAGTGGGTTCATGTCGGATGGTTTGACCGTGACTGCCGATAGCACGGACTTCGTCGGGGCTCATCTGCTGCTGGAGCAGCAGTTCGCGCACGCCCTGTGCTGCAAGGGCGACCTAGCGt                                                                                                                                                >  1:1357777/1‑151 (MQ=255)
    gCGGGGTTGCCGATCTGCACCGTGAAGTGGCGAGTGGGTTCATGTCGGATGGTTTGACCGTGACTGCCGATAGCACGGACTTCGTCGGGGCTCATCTGCTGCTGGAGCAGCAGTTCGCGCACGCCCTGTGCTGCAAGGGCGACCTAGCGtt                                                                                                                                               <  1:8970963/151‑1 (MQ=255)
    gCGGGGTTGCCGATCTGCACCGTGAAGTGGCGAGTGGGTTCATGTCGGATGGTTTGACCGTGACTGCCGATAGCACGGACTTCGTCGGGGCTCATCTGCTGCTGGAGCAGCAGTTCGCGCACGCCCTGTGCTGCAAGGGCGACCTAGCGtt                                                                                                                                               <  1:9809030/151‑1 (MQ=255)
     cGGGGTTGCCGATCTGCACCGTGAAGTGGCGAGTGGGTTCATGTCGGATGGTTTGACCGTGACTGCCGATAGCACGGACTTCGTCGGGGCTCATCTGCTGCTGGAGCAGCAGTTCGCGCACGCCCTGTGCTGCAAGGGCGACCTAGCGTTg                                                                                                                                              <  1:11164026/151‑1 (MQ=255)
        ggTTGCCGATCTGCACCGTGAAGTGGCGAGTGGGTTCATGTCGGATGGTTTGACCGTGACTGCCGATAGCACGGACTTCGTCGGGGCTCATCTGCTGCTGGAGCAGCAGTTCGCGCACGCCCTGTGCTGCAAGGGCGACCTAGCGTTGCTc                                                                                                                                           <  2:7506762/151‑1 (MQ=255)
         gTTGCCGATCTGCTCCGTGAAGTGGCGAGTGGGTTCATGTCGGATGGTTTGACCGTGACTGCCGATAGCACGGACTTCGTCGGGGCTCATCTGCTGCTGGAGCAGCAGTTCGCGCACGCCCTGTGCTGCAAGGGCGACCTAGCGTTGCTCg                                                                                                                                          <  1:491338/151‑1 (MQ=255)
         gTTGCCGATCTGCACCGTGAAGTGGCGAGTGGGTTCATGTCGGATGGTTTGACCGTGACTGCCGATAGCACGGACTTCGTCGGGGCTCATCTGCTGCTGGAGCAGCAGTTCGCGCACGCCCTGTGCTGCAAGGGCGACCTAGCGTTGCTCg                                                                                                                                          <  2:659309/151‑1 (MQ=255)
         gTTGCCGATCTGCACCGTGAAGTGGCGAGTGGGTTCATGTCGGATGGTTTGACCGTGACTGCCGATAGCACGGACTTCGTCGGGGCTCATCTGCTGCTGGAGCAGCAGTTCGCGCACGCCCTGTGCTGCAAGGGCGACCTAGCGTTGCTCg                                                                                                                                          <  2:4830759/151‑1 (MQ=255)
         gTTGCCGATCTGCACCGTGAAGTGGCGAGTGGGTTCATGTCGGATGGTTTGACCGTGACTGCCGATAGCACGGACTTCGTCGGGGCTCATCTGCTGCTGGAGCAGCAGTTCGCGCACGCCCTGTGCTGCAAGGGCGACCTAGCGTTGCTCg                                                                                                                                          <  2:2029769/151‑1 (MQ=255)
         gTTGCCGATCTGCACCGTGAAGTGGCGAGTGGGTTCATGTCGGATGGTTTGACCGTGACTGCCGATAGCACGGACTTCGTCGGGGCTCATCTGCTGCTGGAGCAGCAGTTCGCGCACGCCCTGTGCTGCAAGGGCGACCTAGCGTTGCTCg                                                                                                                                          >  1:7609458/1‑151 (MQ=255)
            gCCGATCTGCACCGTGAAGTGGCGAGTGGGTTCATGTCGGATGGTTTGACCGTGACTGCCGATAGCACGGACTTCGTCGGGGCTCATCTGCTGCTGGAGCAGCAGTTCGCGCACGCCCTGTGCTGCAAGGGCGACCTAGCGTTGCTCGACt                                                                                                                                       >  1:2155611/1‑151 (MQ=255)
                aTCTGCACCGTGAAGTGGCGAGTGGGTTCATGTCGGATGGTTTGACCGTGACTGCCGATAGCACGGACTTCGTCGGGGCTCATCTGCTGCTGGAGCAGCAGTTCGCGCACGCCCTGTGCTGCAAGGGCGACCTAGCGTTGCTCGACTTcgg                                                                                                                                   <  1:772562/151‑1 (MQ=255)
                 tCTGCACCGTGAAGTGGCGAGTGGGTTCATGTCGGATGGTTTGACCGTGACTGCCGATAGCACGGACTTCGTCGGGGCTCATCTGCTGCTGGAGCAGCAGTTCGCGCACGCCCTGTGCTGCAAGGGCGACCTAGCGTTGCTCGACTTcggc                                                                                                                                  <  2:3664245/151‑1 (MQ=255)
                    gCACCGTGAAGTGGCGAGTGGGTTCATGTCGGATGGTTTGACCGTGACTGCCGATAGCACGGACTTCGTCGGGGCTCATCTGCTGCTGGAGCAGCAGTTCGCGCACGCCCTGTGCTGCAAGGGCGACCTAGCGTTGCTCGACTTCggcggc                                                                                                                               <  2:10160144/151‑1 (MQ=255)
                        cGTGAAGTGGCGAGTGGGTTCATGTCGGATGGTTTGACCGTGACTGCCGATAGCACGGACTTCGTCGGGGCTCATCTGCTGCTGGAGCAGCAGTTCGCGCACGCCCTGTGCTGCAAGGGCGACCTAGCGTTGCTCGACTTCGGCGGCGCgg                                                                                                                           <  1:10364874/151‑1 (MQ=255)
                              gTGGCGAGTGGGTTCATGTCGGATGGTTTGACCGTGACTGCCGATAGCACGGACTTCGTCGGGGCTCATCTGCTGCTGGAGCAGCAGTTCGCGCACGCCCTGTGCTGCAAGGGCGACCTAGCGTTGCTCGACTTCGGCGGCGCGGGCGAtt                                                                                                                     >  1:1755288/1‑151 (MQ=255)
                               tGGCGAGTGGGTTCATGTCGGATGGTTTGACCGTGACTGCCGATAGCACGGACTTCGTCGGGGCTCATCTGCTGCTGGAGCAGCAGTTCGCGCACGCCCTGTGCTGCAAGGGCGACCTAGCGTTGCTCGACTTCGGCGGCGCGGGCGAttt                                                                                                                    <  1:1954974/151‑1 (MQ=255)
                                 gCGAGTGGGTTCATGTCGGATGGTTTGACCGTGACTGCCGATAGCACGGACTTCGTCGGGGCTCATCTGCTGCTGGAGCAGCAGTTCGCGCACGCCCTGTGCTGCAAGGGCGACCTAGCGTTGCTCGACTTCGGCGGCGCGGGCGATTTCa                                                                                                                  <  1:8155341/151‑1 (MQ=255)
                                 gCGAGTGGGTTCATGTCGGATGGTTTGACCGTGACTGCCGATAGCACGGACTTCGTCGGGGCTCATCTGCTGCTGGAGCAGCAGTTCGCGCACGCCCTGTGCTGCAAGGGCGACCTAGCGTTGCTCGACTTCGGCGGCGCGGGCGATTTCa                                                                                                                  >  1:10480939/1‑151 (MQ=255)
                                   gAGTGGGTTCATGTCGGATGGTTTGACCGTGACTGCCGATAGCACGGACTTCGTCGGGGCTCATCTGCTGCTGGAGCAGCAGTTCGCGCACGCCCTGTGCTGCAAGGGCGACCTAGCGTTGCTCGACTTCGGCGGCGCGGGCGATTTCATc                                                                                                                <  1:1905791/151‑1 (MQ=255)
                                     gTGGGTTCATGTCGGATGGTTTGACCGTGACTGCCGATAGCACGGACTTCGTCGGGGCTCATCTGCTGCTGGAGCAGCAGTTCGCGCACGCCCTGTGCTGCAAGGGCGACCTCGCGTTGCTCGACTTCGGGGGCGCGGGCGAGTTCTTCgg                                                                                                              >  2:9293361/1‑151 (MQ=255)
                                     gTGGGTTCATGTCGGATGGTTTGACCGTGACTGCCGATAGCACGGACTTCGTCGGGGCTCATCTGCTGCTGGAGCAGCAGTTCGCGCACGCCCTGTGCTGCAAGGGCGACCTAGCGTTGCTCGACTTCGGCGGCGCGGGCGATTTCATCgg                                                                                                              <  1:5184588/151‑1 (MQ=255)
                                         gTTCATGTCGGATGGTTTGACCGTGACTGCCGATAGCACGGACTTCGTCGGGGCTCATCTGCTGCTGGAGCAGCAGTTCGCGCACGCCCTGTGCTGCAAGGGCGACCTAGCGTTGCTCGACTTCGGCGGCGCGGGCGATTTCATCGGGAcc                                                                                                          >  2:8029130/1‑151 (MQ=255)
                                         gTTCATGTCGGATGGTTTGACCGTGACTGCCGATAGCACGGACTTCGTCGGGGCTCATCTGCTGCTGGAGCAGCAGTTCGCGCACGCCCTGTGCTGCAAGGGCGACCTAGCGTTGCTCGACTTCGGCGGCGCGGGCGATTTCATCGGGAcc                                                                                                          <  2:5520297/151‑1 (MQ=255)
                                          ttCATGTCGGATGGTTTGACCGTGACTGCCGATAGCACGGACTTCGTCGGGGCTCATCTGCTGCTGGAGCAGCAGTTCGCGCACGCCCTGTGCTGCAAGGGCGACCTAGCGTTGCTCGACTTCGGCGGCGCGGGCGATTTCATCGGGACCg                                                                                                         >  2:4662401/1‑151 (MQ=255)
                                           tCATGTCGGATTGTTTGACCGTGACTGCCTATAGCACGGACTTCGTCGGGGCTCATCTGCTGCTGGAGCAGCAGTTCGCGCACGCCCTGTGCTGCAAGGGCGACCTAGCGTTGCTCGACTTCGGCGGCGCGGGCGATTTCATCGGGACCgg                                                                                                        <  1:3737705/151‑1 (MQ=255)
                                               gTCGGATGGTTTGACCTTGACTGCCGATAGCACGGTCTTCTTCGGGGCTCATCTGCTGCTGGAGCAGCAGTTCGCGCACGCCCTGTGCTGCAAGGGCGACCTAGCGTTGCTCGACTTCGGCGGCGCGGGCGATTTCATCGGGACCGGGGAc                                                                                                    <  2:813021/151‑1 (MQ=255)
                                               gTCGGATGGTTTGACCGTGACTGCCGATAGCACGGACTTCGTCGGGGCTCATCTGCTGCTGGAGCAGCAGTTCGCGCACGCCCTGTGCTGCAAGGGCGACCTAGCGTTGCTCGACTTCGGCGGCGCGGGCGATTTCATCGGGACCGGGGAc                                                                                                    <  2:6964509/151‑1 (MQ=255)
                                                tCGGATGGTTTGACCGTGACTGCCGATAGCACGGACTTCGTCGGGGCTCATCTGCTGCTGGAGCAGCAGTTCGCGCACGCCCTGTGCTGCAAGGGCGACCTAGCGTTGCTCGACTTCGGCGGCGCGGGCGATTTCATCGGGACCGGGGACg                                                                                                   <  1:5690719/151‑1 (MQ=255)
                                                  ggATGGTTTGACCGTGACTGCCGATAGCACGGACTTCGTCGGGGCTCATCTGCTGCTGGAGCAGCAGTTCGCGCACGCCCTGTGCTGCAAGGGCGACCTAGCGTTGCTCGACTTCGGCGGCGCGGGCGATTTCATCGGGACCGGGGACGca                                                                                                 >  2:11461510/1‑151 (MQ=255)
                                                   gATGGTTTGACCGTGACTGCCGATAGCACGGACTTCGTCGGGGCTCATCTGCTGCTGGAGCAGCAGTTCGCGCACGCCCTGTGCTGCAAGGGCGACCTAGCGTTGCTCGACTTCGGCGGCGCGGGCGATTTCATCGGGACCGGGGACGcac                                                                                                <  1:3887542/151‑1 (MQ=255)
                                                    aTGGTTTGACCGTGACTGCCGATAGCACGGACTTCGTCGGGGCTCATCTGCTGCTGGAGCAGCAGTTCGCGCACGCCCTGTGCTGCAAGGGCGACCTAGCGTTGCTCGACTTCGGCGGCGCGGGCGATTTCATCGGGACCGGGGACGcaca                                                                                               >  1:1174482/1‑151 (MQ=255)
                                                     tGGTTTGACCGTGACTGCCGATAGCACGGACTTCGTCGGGGCTCATCTGCTGCTGGAGCAGCAGTTCGCGCACGCCCTGTGCTGCAAGGGCGACCTAGCGTTGCTCGACTTCGGCGGCGCGGGCGATTTCATCGGGACCGGGGACGCACAg                                                                                              <  1:2647189/151‑1 (MQ=255)
                                                     tGGTTTGACCGTGACTGCCGATAGCACGGACTTCGTCGGGGCTCATCTGCTGCTGGAGCAGCAGTTCGCGCACGCCCTGTGCTGCAAGGGCGACCTAGCGTTGCTCGACTTCGGCGGCGCGGGCGATTTCATCGGGACCGGGGACGCACAg                                                                                              <  1:8349186/151‑1 (MQ=255)
                                                     tGGTTTGACCGTGACTGCCGATAGCACGGACTTCGTCGGGGCTCATCTGCTGCTGGAGCAGCAGTTCGCGCACGCCCTGTGCTGCAAGGGCGACCTAGCGTTGCTCGACTTCGGCGGCGCGGGCGATTTCATCGGGACCGGGGACGCACAg                                                                                              <  1:9019320/151‑1 (MQ=255)
                                                     tGGTTTGACCGTGACTGCCGATAGCACGGACTTCGACGGGGCTCATCTGCTGCTGGAGCAGCAGTTCGCGCACGCCCTGTGCTGCAAGGGCGACCTAGCGTTGCTCGACTTCGGCGGCGCGGGCGATTTCCTCGGGACCGGGGACGCACAg                                                                                              <  2:8663440/151‑1 (MQ=255)
                                                      ggTTTGACCGTGACTGCCGATAGCACGGACTTCGTCGGGGCTCATCTGCTGCTGGAGCAGCAGTTCGCGCACGCCCTGTGCTGCAAGGGCGACCTAGCGTTGCTCGACTTCGGCGGCGCGGGCGATTTCATCGGGACCGGGGACGCACAgg                                                                                             <  1:2284277/151‑1 (MQ=255)
                                                      ggTTTGACCGTGACTGCCGATAGCACGGACTTCGTCGGGGCTCATCTGCTGCTGGAGCAGCAGTTCGCGCACGCCCTGTGCTGCAAGGGCGACCTAGCGTTGCTCGACTTCGGCGGCGCGGGCGATTTCATCGGGACCGGGGACGCACAgg                                                                                             <  1:8526404/151‑1 (MQ=255)
                                                        tttGACCGTGACTGCCGATCGCACGGACTTCGTCGGGGCTCATCTGCTGCTGGAGCAGCAGTTCGCGCACGCCCTGTGCTGCAAGGGCGACCTAGCGTTGCTCGACTTCGGCGGCGCGGGCGATTTCATCGGGACCGGGGACGCACAGGGc                                                                                           <  2:5336372/151‑1 (MQ=255)
                                                        tttGACCGTGACTGCCGATAGCACGGACTTCGTCGGGGCTCATCTGCTGCTGGAGCAGCAGTTCGCGCACGCCCTGTGCTGCAAGGGCGACCTAGCGTTGCTCGACTTCGGCGGCGCGGGCGATTTCATCGGGACCGGGGACGCACAGGGc                                                                                           <  1:7189437/151‑1 (MQ=255)
                                                           gACCGTGACTGCCGATAGCACGGACTTCGTCGGGGCTCATCTGCTGCTGGAGCAGCAGTTCGCGCACGCCCTGTGCTGCAAGGGCGACCTAGCGTTGCTCGACTTCGGCGGCGCGGGCGATTTCATCGGGACCGGGGACGCACAGGGCGAg                                                                                        >  2:10775067/1‑151 (MQ=255)
                                                           gACCGTGACTGCCGATAGCACGGACTTCGTCGGGGCTCATCTGCTGCTGGAGCAGCAGTTCGCGCACGCCCTGTGCTGCAAGGGCGACCTAGCGTTGCTCGACTTCGGCGGCGCGGGCGATTTCATCGGGACCGGGGACGCACAGGGCGAg                                                                                        <  1:11371809/151‑1 (MQ=255)
                                                                 gACTGCCGATAGCACGGACTTCGTCGGGGCTCATCTGCTGCTGGAGCAGCAGTTCGCGCACGCCCTGTGCTGCAAGGGCGACCTAGCGTTGCTCGACTTCGGCGGCGCGGGCGATTTCATCGGGACCGGGGACGCACAGGGCGAGGATGTc                                                                                  >  2:421923/1‑151 (MQ=255)
                                                                  aCTGCCGATAGCACGGACTTCGTCGGGGCTCATCTGCTGCTGGAGCAGCAGTTCGCGCACGCCCTGTGCTGCAAGGGCGACCTAGCGTTGCTCGACTTCGGCGGCGCGGGCGATTTCATCGGGACCGGGGACGCACAGGGCGAGGATGTCt                                                                                 >  2:11271875/1‑151 (MQ=255)
                                                                   cTGCCGATAGCACGGACTTCGTCGGGGCTCATCTGCTGCTGGAGCAGCTGTTCGCGCACGCCCTGTGCTGCAAGGGCGACCTAGCGTTGCTCGACTTCGGCGGCGCGGGCGATTTCATCGGGACCGGGGACGCACAGGGCGAGGATGTCtt                                                                                <  2:2301322/151‑1 (MQ=255)
                                                                   cTGCCGATAGCACGGACTTCGTCGGGGCTCATCTGCTGCTGGAGCAGCAGTTCGCGCACGCCCTGTGCTGCAAGGGCGACCTAGCGTTGCTCGACTTCGGCGGCGCGGGCGATTTCATCGGGACCGGGGACGCACAGGGCGAGGATGTCtt                                                                                <  1:5922966/151‑1 (MQ=255)
                                                                   cTGCCGATAGCACGGACTTCGTCGGGGCTCATCTGCTGCTGGAGCAGCAGTTCGCGCACGCCCTGTGCTGCAAGGGCGACCTAGCGTTGCTCGACTTCGGCGGCGCGGGCGATTTCATCGGGACCGGGGACGCACAGGGCGAGGATGTCtt                                                                                >  1:4599861/1‑151 (MQ=255)
                                                                    tGCCGATAGCACGGACTTCGTCGGGGCTCATCTGCTGCTGGAGCAGCAGTTCGCGCACGCCCTGTGCTGCAAGGGCGACCTAGCGTTGCTCGACTTCGGCGGCGCGGGCGATTTCATCGGGACCGGGGACGCACAGGGCGAGGATGTCTTc                                                                               <  1:10855335/151‑1 (MQ=255)
                                                                    tGCCGATAGCACGGACTTCGTCGGGGCTCATCTGCTGCTGGAGCAGCAGTTCGCGCACGCCCTGTGCTGCAAGGGCGACCTAGCGTTGCTCGACTTCGGCGGCGCGGGCGATTTCATCGGGACCGGGGACGCACAGGGCGAGGATGTCTTc                                                                               >  2:3229987/1‑151 (MQ=255)
                                                                          tAGCANGGACTTCGTCGGGGCTCATCCGCTGCTGGAGCAGCAGTTCGCGCACGCCCTGTGCTGCAAGGGCGACCTAGCGTTGCTCGACTTCGGCGGCGCGGGCGATTTCATCGGGACCGGGGACGCACAGGGCGAGGATGTCTTCGCGCAg                                                                         >  1:6080647/1‑151 (MQ=255)
                                                                             cACGGACTTCGTCGGGGCTCATCTGCTGCTGGAGCAGCAGTTCGCGCACGCCCTGTGCTGCAAGGGCGACCTAGCGTTGCTCGACTTCGGCGGCGCGGGCGATTTCATCGGGACCGGGGACGCACAGGGCGAGGATGTCTTCGCGCAGccc                                                                      >  1:7022330/1‑151 (MQ=255)
                                                                             cACGGACTTCGTCGGGGCTCATCTGCTGCTGGAGCAGCAGTTCGCGCACGCCCTGTGCTGCAAGGGCGACCTAGCGTTGCTCGACTTCGGCGGCGCGGGCGATTTCATCGGGACCGGGGACGCACAGGGCGAGGATGTCTTCGCGCAGccc                                                                      <  2:9052739/151‑1 (MQ=255)
                                                                              aCGGACTTCGTCGGGGCTCATCTGCTGCTGGAGCAGCAGTTCGCGCACGCCCTGTGCTGCAAGGGCGACCTAGCGTTGCTCGACTTCGGCGGCGCGGGCGATTTCATCGGGACCGGGGACGCACAGGGCGAGGATGTCTTCGCGCAGCccg                                                                     >  1:9234695/1‑151 (MQ=255)
                                                                               cGGACTTCGTCGGGGCTCATCTGCTGCTGGAGCAGCAGTTCGCGCACGCCCTGTGCTGCAAGGGCGACCTAGCGTTGCTCGACTTCGGCGGCGCGGGCGATTTCATCGGGACCGGGGACGCACAGGGCGAGGATGTCTTCGCGCAGCccgg                                                                    >  1:8817876/1‑151 (MQ=255)
                                                                                ggACTTCGTCGGGGCTCATCTGCTGCTGGAGCAGCAGTTCGCGCACGCCCTGTGCTGCAAGGGCGACCTAGCGTTGCTCGACTTCGGCGGCGCGGGCGATTTCATCGGGACCGGGGACGCACAGGGCGAGGATGTCTTCGCGCAGCccggc                                                                   >  2:10885677/1‑151 (MQ=255)
                                                                                ggACTTCGTCGGGGCTCATCTGCTGCAGGAGCAGCAGTTCGCGCACGCCCTGTGCTGCAAGGGCGACCTAGCGTTGCTCGACTTCGGCGGCGCGGGCGATTTCATCGGGACCGGGGACGCACAGGGCGAGGATGTCTTCGCGCAGCccggc                                                                   <  1:1544190/151‑1 (MQ=255)
                                                                                 gACTTCGTCGGGGCTCATCTGCTGCTGGAGCAGCAGTTCGCGCACGCCCTGTGCTGCAAGGGCGACCTAGCGTTGCTCGACTTCGGCGGCGCGGGCGATTTCATCGGGACCGGGGACGCACAGGGCGAGGATGTCTTCGCGCAGCccggcc                                                                  >  1:4392592/1‑151 (MQ=255)
                                                                                      cgtcgGGGCTCATCTGCTGCTGGAGCAGCAGTTCGCGCACGCCCTGTGCTGCAAGGGCGACCTAGCGTTGCTCGACTTCGGCGGCGCGGGCGATTTCATCGGGACCGGGGACGCACAGGGCGAGGATGTCTTCGCGCAGCCCGGCCGGCAt                                                             >  2:6455389/1‑151 (MQ=255)
                                                                                       gtcgGGGCTCATCTGCTGCTGGAGCAGCAGTTCGCGCACGCCCTGTGCTGCAAGGGCGACCTAGCGTTGCTCGACTTCGGCGGCGCGGGCGATTTCATCGGGACCGGGGACGCACAGGGCGAGGATGTCTTCGCGCAGCCCGGCCGGCATg                                                            >  1:7894768/1‑151 (MQ=255)
                                                                                       gtcgGGGCTCATCTGCTGCTGGAGCAGCAGTTCGCGCACGCCCTGTGCTGCAAGGGCGACCTAGCGTTGCTCGACTTCGGCGGCGCGGGCGATTTCATCGGGACCGGGGACGCACAGGGCGAGGATGTCTTCGCGCAGCCCGGCCGGCATg                                                            <  2:2739375/151‑1 (MQ=255)
                                                                                       gtcgGGGCTCATCTGCTGCTGGAGCAGCAGTTCGCGCACGCCCTGTGCTGCAAGGGCGACCTAGCGTTGCTCGACTTCGGCGGCGCGGGCGATTTCATCGGGACCGGGGACGCACAGGGCGAGGATGTCTTCGCGCAGCCCGGCCGGCATg                                                            >  1:9472527/1‑151 (MQ=255)
                                                                                       gtcgGGGCTCATCTGCTGCTGGAGCAGCAGTTCGCGCACGCCCCGTGCTGCAAGGGCGACCTAGCGTTGCTCGACTTCGGCGGCGCGGGCGATTTCATCGGGACCGGGGACGCACAGGGCGAGGATGTCTTCGCGCAGCCCGGCCGGCATg                                                            >  2:9646952/1‑151 (MQ=255)
                                                                                         cgGGGCTCATCTGCTGCTGGAGCAGCAGTTCGCGCACGCCCTGTGCTGCAAGGGCGACCTAGCGTTGCTCGACTTCGGCGGCGCGGGCGATTTCATCGGGACCGGGGACGCACAGGGCGAGGATGTCTTCGCGCAGCCCGGCCGGCATggg                                                          <  2:3317042/151‑1 (MQ=255)
                                                                                          ggggCTCATCTGCTGCTGGAGCAGCAGTTCGCGCACGCCCTGTGCTGCAAGGGCGACCTAGCGTTGCTCGACTTCGGCGGCGCGGGCGATTTCATCGGGACCGGGGACGCACAGGGCGAGGATGTCTTCGCGCAGCCCGGCCGGCATGggt                                                         <  1:5419186/151‑1 (MQ=255)
                                                                                          ggggCTCATCTGCTGCTGGAGCAGCAGTTCGCGCACGCCCTGTGCTGCAAGGGCGACCTAGCGTTGCTCGACTTCGGCGGCGCGGGCGATTTCATCGGGACCGGGGACGCACAGGGCGAGGATGTCTTCGCGCAGCCCGGCCGGCATGggt                                                         <  1:2889131/151‑1 (MQ=255)
                                                                                          ggggCTCATCTGCTGCTGGAGCAGCAGTTCGCGCACGCCCTGTGCTGCAAGGGCGACCTAGCGTTGCTCGACTTCGGCGGCGCGGGCGATTTCATCGGGACCGGGGACGCACAGGGCGAGGATGTCTTCGCGCAGCCCGGCCGGCATGggt                                                         >  1:6367824/1‑151 (MQ=255)
                                                                                            ggCTCATCTGCTGCTGGAGCAGCAGTTCGCGCACGCCCTGTGCTGCAAGGGCGACCTAGCGTTGCTCGACTTCGGCGGCGCGGGCGATTTCATCGGGACCGGGGACGCACAGGGCGAGGATGTCTTCGCGCAGCCCGGCCGGCATGggtag                                                       >  2:9485340/1‑151 (MQ=255)
                                                                                            ggCTCATCTGCTGCTGGAGCAGCAGTTCGCGCACGCCCTGTGCTGCAAGGGCGACCTAGCGTTGCTCGACTTCGGCGGCGCGGGCGATTTCATCGGGACCGGGGACGCACAGGGCGAGGATGTCTTCGCGCAGCCCGGCCGGCATGggtag                                                       >  2:7777279/1‑151 (MQ=255)
                                                                                               tCATCTGCTGCTGGAGCAGCAGTTCGCGCACGCCCTGTGCTGCAAGGGCGACCTAGCGTTGCTCGACTTCGGCGGCGCGGGCGATTTCATCGGGACCGGGGACGCACAGGGCGAGGATGTCTTCGCGCAGCCCGGCCGGCATGggtaggta                                                    >  1:4803164/1‑151 (MQ=255)
                                                                                                cATCTGCTGCTGGAGCAGCAGTTCGCGCACGCCCTGTGCTGCAAGGGCGACCTAGCGTTGCTCGACTTCGGCGGCGCGGGCGATTTCATCGGGACCGGGGACGCACAGGGCGAGGATGTCTTCGCGCAGCCCGGCCGGCATGGGTAGGTaa                                                   <  2:1712573/151‑1 (MQ=255)
                                                                                                 aTCTGCTGCTGGAGCAGCAGTTCGCGCACGCCCTGTGCTGCAAGGGCGACCTAGCGTTGCTCGACTTCGGCGGCGCGGGCGATTTCATCGGGACCGGGGACGCACAGGGCGAGGATGTCTTCGCGCAGCCCGGCCGGCATGGGTAGGTAAt                                                  <  2:1443874/151‑1 (MQ=255)
                                                                                                     gctgctgGAGCAGCAGTTCGCGCACGCCCTGTGCTGCAAGGGCGACCTAGCGTTGCTCGACTTCGGCGGCGCGGGCGATTTCATCGGGACCGGGGACGCACAGGGCGAGGATGTCTTCGCGCAGCCCGGCCGGCATGGGTAGGTAATGGGa                                              <  1:7310072/151‑1 (MQ=255)
                                                                                                      ctgctgGAGCAGCAGTTCGCGCACGCCCTGTGCTGCAAGGGCGACCTAGCGTTGCTCGACTTCGGCGGCGCGGGCGATTTCATCGGGACCGGGGACGCACAGGGCGAGGATGTCTTCGCGCAGCCCGGCCGGCATGGGTAGGTAATGGGaa                                             <  2:6009795/151‑1 (MQ=255)
                                                                                                       tgctgGAGCAGCAGTTCGCGCACGCCCTGTGCTGCAAGGGCGACCTAGCGTTGCTCGACTTCGGCGGCGCGGGCGATTTCATCGGGACCGGGGACGCACAGGGCGAGGATGTCTTCGCGCAGCCCGGCCGGCATGGGTAGGTAATGGGAAg                                            <  2:3957700/151‑1 (MQ=255)
                                                                                                         ctgGAGCAGCAGTTCGCGCACGCCCTGTGCTGCAAGGGCGACCTAGCGTTGCTCGACTTCGGCGGCGCGGGCGATTTCATCGGGACCGGGGACGCACAGGGCGAGGATGTCTTCGCGCAGCCCGGCCGGCATGGGTAGGTAATGGGAAGCg                                          <  1:4274677/151‑1 (MQ=255)
                                                                                                          tgGAGCAGCAGTTCGCGCACGCCCTGTGCTGCAAGGGCGACCTAGCGTTGCTCGACTTCGGCGGCGCGGGCGATTTCATCGGGACCGGGGACGCACAGGGCGAGGATGTCTTCGCGCAGCCCGGCCGGCATGGGTAGGTAATGGGAAGCGa                                         <  1:3236356/151‑1 (MQ=255)
                                                                                                             agcagcAGTTCGCGCACGCCCTGTGCTGCAAGGGCGACCTAGCGTTGCTCGACTTCGGCGGCGCGGGCGATTTCATCGGGACCGGGGACGCACAGGGCGAGGATGTCTTCGCGCAGCCCGGCCGGCATGGGTAGGTCATGGGAAGCGAGCa                                      >  2:8451577/1‑151 (MQ=255)
                                                                                                                agcagTTCGCGCACGCCCTGTGCTGCAAGGGCGACCTAGCGTTGCTCGACTTCGGCGGCGCGGGCGATTTCATCGGGACCGGGGACGCACAGGGCGAGGATGTCTTCGCGCAGCCCGGCCGGCATGGGTAGGTAATGGGAAGCGAGCAAgg                                   >  1:6215303/1‑151 (MQ=255)
                                                                                                                  cagTTCGCGCACGCCCTGTGCTGCAAGGGCGACCTAGCGTTGCTCGACTTCGGCGGCGCGGGCGATTTCATCGGGACCGGGGACGCACAGGGCGAGGATGTCTTCGCGCAGCCCGGCCGGCATGGGTAGGTAATGGGAAGCGAGCAAggtg                                 <  1:4106067/151‑1 (MQ=255)
                                                                                                                    gTTCGCGCACGCCCTGTGCTGCAAGGGCGACCTAGCGTTGCTCGACTTCGGCGGCGCGGGCGATTTCATCGGGACCGGGGACGCACAGGGCGAGGATGTCTTCGCGCAGCCCGGTCGGCATGGGTAGGTAATGGGAAGCGAGCAAggtggt                               <  2:8846126/151‑1 (MQ=255)
                                                                                                                      tCGCGCACGCCCTGTGCTGCAAGGGCGACCTAGCGTTGCTCGACTTCGGCGGCGCGGGCGATTTCATCGGGACCGGGGACGCACAGGGCGAGGATGTCTTCGCGCAGCCCGGCCGGCATGGGTAGGTAATGGGAAGCGAGCAAGGTGGTGc                             <  2:621862/151‑1 (MQ=255)
                                                                                                                       cgcgCACGCCCTGTGCTGCAAGGGCGACCTAGCGTTGCTCGACTTCGGCGGCGCGGGCGATTTCATCGGGACCGGGGACGCACAGGGCGAGGATGTCTTCGCGCAGCCCGGCCGGCATGGGTAGGTAATGGGAAGCGAGCACGGTGGTGCg                            >  2:8193601/1‑151 (MQ=255)
                                                                                                                       cgcgCACGCCCTGTGCTGCAAGGGCGACCTAGCGTTGCTCGACTTCGGCGGCGCGGGCGATTTCATCGGGACCGGGGACGCACAGGGCGAGGATGTCTTCGCGCAGCCCGGCCGGCATGGGTAGGTAATGGGAAGCGAGCAAGGTGGTGCg                            >  1:304910/1‑151 (MQ=255)
                                                                                                                        gcgcACGCCCTGTGCTGCAAGGGCGACCTAGCGTTGCTCGACTTCGGCGGCGCGGGCGATTTCATCGGGACCGGGGACGCACAGGGCGAGGATGTCTTCGCGCAGCCCGGCCGGCATGGGTAGGTAATGGGCAGCGAGCAAGGTGGTGCGa                           >  2:2546069/1‑151 (MQ=255)
                                                                                                                        gcgcACGCCCTGTGCTGCAAGGGCGACCTAGCGTTGCTCGACTTCGGCGGCGCGGGCGATTTCATCGGGACCGGGGACGCACAGGGCGAGGATGTCTTCGCGCAGCCCGGCCGGCATGGGTAGGTAATGGGAAGCGAGCAAGGTGGTGCGa                           <  1:10635285/151‑1 (MQ=255)
                                                                                                                         cgcACGCCCTGTGCTGCAAGGGCGACCTAGCGTTGCTCGACTTCGGCGGCGCGGGCGATTTCATCGGGACCGGGGACGCACAGGGCGAGGATGTCTTCGCGCAGCCCGGCCGGCATGGGTAGGTAATGGGAAGCGAGCAAGGTGGTGCGAt                          >  2:10650760/1‑151 (MQ=255)
                                                                                                                          gcACGCCCTGTGCTGCAAGGGCGACCTAGCGTTGCTCGACTTCGGCGGCGCGGGCGATTTCATCGGGACCGGGGACGCACAGGGCGAGGATGTCTTCGCGCAGCCCGGCCGGCATGGGTAGGTAATGGGATGCGAGCAAGGTGGTGCGATc                         >  2:3041598/1‑151 (MQ=255)
                                                                                                                          gcACGCCCTGTGCTGCAAGGGCGACCTAGCGTTGCTCGACTTCGGCGGCGCGGGCGATTTCATCGGGACCGGGGACGCACAGGGCGAGGATGTCTTCGCGCAGCCCGGCCGGCATGGGTAGGTAATGGGAAGCGAGCAAGGTGGTGCGATc                         >  2:4874578/1‑151 (MQ=255)
                                                                                                                            aCGCCCTGTGCTGCAAGGGCGACCTAGCGTTGCTCGACTTCGGCGGCGCGGGCGATTTCATCGGGACCGGGGACGCACAGGGCGAGGATGTCTTCGCGCAGCCCGGCCGGCATGGGTAGGTAATGGGAAGCGAGCAAGGTGGTGCGATCAc                       <  2:187497/151‑1 (MQ=255)
                                                                                                                                 cTGTGCTGCAAGGGCGACCTAGCGTTGCTCGACTTCGGCGGCGCGGGCGATTTCATCGGGACCGGGGACGCACAGGGCGAGGATGTCTTCGCGCAGCCCGGCCGGCATGGGTAGGTAATGGGAAGCGAGCAAGGTGGTGCGATCACCTTGc                  <  1:6810421/151‑1 (MQ=255)
                                                                                                                                  tgtgCTGCAAGGGCGACCTAGCGTTGCTCGACTTCGGCGGCGCGGGCGATTTCATCGGGACCGGGGACGCACAGGGCGAGGATGTCTTCGCGCAGCCCGGCCGGCATGGGTAGGTAATGGGAAGCGAGCAAGGTGGTGCGATCACCTTGCt                 >  2:7140162/1‑151 (MQ=255)
                                                                                                                                    tgctgcAAGGGCGACCTAGCGTTGCTCGACTTCGGCGGCGCGGGCGATTTCATCGGGACCGGGGACGCACAGGGCGAGGATGTCTTCGCGCAGCCCGGCCGGCATGGGTAGGTCATGGGAAGCGAGCAAGGTGGTGCGATCACCTTGCTCg               >  2:5855632/1‑151 (MQ=255)
                                                                                                                                      ctgcAAGGGCGACCTAGCGTTGCTCGACTTCGGCGGCGCGGGCGATTTCATCGGGACCGGGGACGCACAGGGCGAGGATGTCTTCGCGCAGCCCGGCCGGCATGGGTAGGTAATGGGAAGCGAGCAAGGTGGTGCGATCACCTTGCTCGAt             >  2:5073178/1‑151 (MQ=255)
                                                                                                                                       tgcAAGGGCGACCTAGCGTTGCTCGACTTCGGCGGCGCGGGCGATTTCATCGGGACCGGGGACGCACAGGGCGAGGATGTCTTCGCGCAGCCCGGCCGGCATGGGTAGGTAATGGGAAGCGAGCAAGGTGGTGCGATCACCTTGCTCGATc            <  1:10107485/151‑1 (MQ=255)
                                                                                                                                               cGACCTAGCGTTGCTCGACTTCGGCGGCGCGGGCGATTTCATCGGGACCGGGGACGCACAGGGCGAGGATGTCTTCGCGCAGCCCGGCCGGCATGGGTAGGTAATGGGAAGCGAGCAAGGTGGTGCGATCACCTTGCTCGATCAGGACGAt    <  1:3528242/151‑1 (MQ=255)
                                                                                                                                               cGACCTAGCGTTGCTCGACTTCGGCGGCGCGGGCGATTTCATCGGGACCGGGGACGCACAGGGCGAGGATGTCTTCGCGCAGCCCGGCCGGCATGGGTAGGTAATGGGAAGCGAGCAAGGTGGTGCGATCACCTTGCTCGATCAGGACGAt    >  2:11086237/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CAGGGCGGGGTTGCCGATCTGCACCGTGAAGTGGCGAGTGGGTTCATGTCGGATGGTTTGACCGTGACTGCCGATAGCACGGACTTCGTCGGGGCTCATCTGCTGCTGGAGCAGCAGTTCGCGCACGCCCTGTGCTGCAAGGGCGACCCAGCGTTGCTCGACTTCGGCGGCGCGGGCGATTTCATCGGGACCGGGGACGCACAGGGCGAGGATGTCTTCGCGCAGCCCGGCCGGCATGGGTAGGTAATGGGAAGCGAGCAAGGTGGTGCGATCACCTTGCTCGATCAGGACGATGT  >  NC_008463/729372‑729667

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑