Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 6,076,066 G→C R105R (CGG→CGC)  PA14_RS27775 → methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084636,076,0660GC99.8% 2241.7 / NA 584R105R (CGG→CGC) PA14_RS27775methyltransferase
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base C (292/291);  total (293/291)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 584 total aligned reads displayed.

GGCGCAGCCTCGAACAGGCCGACGCGGAGCACAACCAGCCCACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGGTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCTGGGCCGCCTGCTGGCCCATGCGGGGCAACCG  >  NC_008463/6075918‑6076213
                                                                                                                                                    |                                                                                                                                                   
 gcgcAGCCTCGAACAGGCCGACGCGGAGCACAACCAGCCCACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTc                                                                                                                                                  <  1:9388665/151‑1 (MQ=255)
  cgcAGCCTCGAACAGGCCGACGCGGAGCACAACCAGCCCACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCa                                                                                                                                                 <  2:111477/151‑1 (MQ=255)
  cgcAGCCTCGAACAGGCCGACGCGGAGCACAACCAGCCCACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCa                                                                                                                                                 >  1:7366112/1‑151 (MQ=255)
   gcAGCCTCGAACAGGCCGACGCGGAGCACAACCAGCCCACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCaa                                                                                                                                                <  1:3223170/151‑1 (MQ=255)
   gcAGCCTCGAACAGGCCGACGCGGAGCACAACCAGCCCACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCaa                                                                                                                                                <  1:2433907/151‑1 (MQ=255)
      gCCTCGAACAGGCCGACGCGGAGCACAACCAGCCCACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGt                                                                                                                                             <  2:5678685/151‑1 (MQ=255)
       ccTCGAACAGGCCGACGCGGAGCACAACCAGCCCACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTc                                                                                                                                            <  2:5879865/151‑1 (MQ=255)
          cGAACAGGCCGACGCGGAGCACAACCAGCCCACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGAc                                                                                                                                         <  2:10401108/151‑1 (MQ=255)
           gAACAGGCCGACGCGGAGCACAACCAGCCCACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACg                                                                                                                                        >  2:9142943/1‑151 (MQ=255)
            aaCAGGCCGACGCGGAGCACAACCAGCCCACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGGCGGCGAACTGCCATTGGCGGAACGCCCGCGGGCCCCGAACCGCTTCAATGTCGACGt                                                                                                                                       >  1:137974/1‑151 (MQ=255)
              cAGGCCGACGCGGAGCACAACCAGCCCACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTAc                                                                                                                                     >  2:7159777/1‑151 (MQ=255)
               aGGCCGACGCGGAGCACAACCAGCCCACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAGCCGCTTCAATGTCGACGTAcc                                                                                                                                    >  2:5993382/1‑151 (MQ=255)
                ggCCGACGCGGAGCACAACCAGCCCACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCg                                                                                                                                   <  1:11147679/151‑1 (MQ=255)
                ggCCGACGCGGAGCACAACCAGCCCACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCg                                                                                                                                   <  1:5980306/151‑1 (MQ=255)
                ggCCGACGCGGAGCACAACCAGCCCACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCg                                                                                                                                   <  2:4058590/151‑1 (MQ=255)
                 gCCGACGCGGAGCACAACCAGCCCACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCgg                                                                                                                                  <  2:1614587/151‑1 (MQ=255)
                  ccGACGCGGAGCACAACCAGCCCACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCggg                                                                                                                                 <  1:10010429/151‑1 (MQ=255)
                    gACGCGGAGCACAACCAGCCCACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGAc                                                                                                                               <  1:5017416/151‑1 (MQ=255)
                    gACGCGGAGCACAACCAGCCCACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGAc                                                                                                                               <  1:3672860/151‑1 (MQ=255)
                            gCACAACCAGCCCCCCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCGCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCAGTGGCGGACCGCCCGCAGGCACCGAACCGCGTCAATGGCGACGTCCCGGGACGCAAGTgg                                                                                                                       >  2:7887810/1‑151 (MQ=255)
                              acaACCAGCCCACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGgca                                                                                                                     <  2:5620204/151‑1 (MQ=255)
                               caACCAGCCCACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCCTTGGCGGAACGCCCGCAGGCACCGAACCGCTGCAATGTCGACGTACCGGGACGCTAGTGgcag                                                                                                                    >  1:8068388/1‑151 (MQ=255)
                                aaCCAGCCCACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGgcagc                                                                                                                   <  1:9135494/151‑1 (MQ=255)
                                 aCCAGCCCACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGgcagca                                                                                                                  <  2:463327/151‑1 (MQ=255)
                                 aCCAGCCCACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGgcagca                                                                                                                  >  1:10250987/1‑151 (MQ=255)
                                   cAGCCCACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGa                                                                                                                >  2:8187960/1‑151 (MQ=255)
                                   cAGCCCACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGGCGACGTACCGGGACGCAAGTGGCAGCAGa                                                                                                                >  2:9396834/1‑151 (MQ=255)
                                   cAGCCCACCCGCCTGGCCGCGACCTCGCCGTATCGGCAGCTCCCCGCCCAGGCGGCCGGGGGCGGGGCGGGGGGCGCACTGCGTTGGGCGGAACGCGCGCGGGCTCCGATCGGCTTCAATCTCGACGGACGGGGACGCACGGGGaagcagt                                                                                                                >  2:3874463/1‑150 (MQ=255)
                                    aGCCCACCCGCCTGGCCGCGCCCGCGCCGTCTCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGAGGCAGCAGAt                                                                                                               >  2:10062665/1‑151 (MQ=255)
                                       ccACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCgg                                                                                                            <  1:10470469/151‑1 (MQ=255)
                                         aCCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGcc                                                                                                          >  2:5272348/1‑151 (MQ=255)
                                           ccGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTg                                                                                                        <  1:4803440/151‑1 (MQ=255)
                                             gCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCACGTGGCAGCAGATCGGCCTGtt                                                                                                      >  2:1413476/1‑151 (MQ=255)
                                             gCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGtt                                                                                                      <  1:1935448/151‑1 (MQ=255)
                                                tGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGc                                                                                                   <  2:7411800/151‑1 (MQ=255)
                                                tGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGc                                                                                                   <  2:10794369/151‑1 (MQ=255)
                                                 ggCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCCTTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGcc                                                                                                  >  1:1760763/1‑151 (MQ=255)
                                                 ggCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGcc                                                                                                  <  1:2534091/151‑1 (MQ=255)
                                                     gcgcccgcgccTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGgc                                                                                              <  1:7294853/151‑1 (MQ=255)
                                                      cgcccgcgccTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGgcg                                                                                             <  1:4123664/151‑1 (MQ=255)
                                                       gcccgcgccTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGgcgc                                                                                            >  2:6211844/1‑151 (MQ=255)
                                                       gcccgcgccTGCTCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGGGGCAGCAGCTCGGCCTGTTCGCCGGgcgc                                                                                            <  2:7525706/151‑1 (MQ=255)
                                                         ccgcgccTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCt                                                                                          >  1:8955937/1‑151 (MQ=255)
                                                         ccgcgccTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCt                                                                                          >  1:9602707/1‑151 (MQ=255)
                                                             gccTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGACCCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAg                                                                                      >  2:6548517/1‑151 (MQ=255)
                                                              ccTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCAACGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATAGGCCTGTTCGCCGGGCGCCTGCAGt                                                                                     >  1:10762182/1‑151 (MQ=255)
                                                               cTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCGGTTCGCCGGGCGCCTGCAGtt                                                                                    >  2:2155057/1‑151 (MQ=255)
                                                                ttATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTc                                                                                   <  2:2735489/151‑1 (MQ=255)
                                                                   tCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGcc                                                                                <  1:8506596/151‑1 (MQ=255)
                                                                   tCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGcc                                                                                <  1:581404/151‑1 (MQ=255)
                                                                     cGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCccg                                                                              >  2:6979113/1‑151 (MQ=255)
                                                                          cTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCccggccgg                                                                         >  2:768068/1‑151 (MQ=255)
                                                                            cgccgcCCAGGCCGCAGCTCTGGGCGCGGTCGGCGAACTGCCATTGGCTGAACGCACGCAGGCACCGAACCGCTTCAATGTCTACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGgtc                                                                       >  2:4834544/1‑151 (MQ=255)
                                                                               cgccCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGgtcgtc                                                                    <  1:4625737/151‑1 (MQ=255)
                                                                                gccCAGGCCGCAGCGCTGGGCGCGGTCGGCGACCTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTcc                                                                   <  1:5094691/151‑1 (MQ=255)
                                                                                gccCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTcc                                                                   <  1:3972987/151‑1 (MQ=255)
                                                                                 cccAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCGGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCa                                                                  >  1:4267695/1‑151 (MQ=255)
                                                                                 cccAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATGGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCa                                                                  <  1:7009775/151‑1 (MQ=255)
                                                                                  ccAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCCCCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCAc                                                                 >  2:9584262/1‑151 (MQ=255)
                                                                                   cAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACt                                                                >  1:3387007/1‑151 (MQ=255)
                                                                                    aGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTg                                                               >  1:693704/1‑151 (MQ=255)
                                                                                       ccGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCt                                                            >  1:2923409/1‑151 (MQ=255)
                                                                                           aGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGAc                                                        <  2:9860540/151‑1 (MQ=255)
                                                                                             cgcTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTg                                                      <  1:5638678/151‑1 (MQ=255)
                                                                                               cTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGt                                                    <  1:8739317/151‑1 (MQ=255)
                                                                                                   gcgcGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTc                                                >  2:2953175/1‑151 (MQ=255)
                                                                                                    cgcgGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTcc                                               <  2:3119373/151‑1 (MQ=255)
                                                                                                    cgcgGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTcc                                               <  1:2028979/151‑1 (MQ=255)
                                                                                                     gcgGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCg                                              <  1:3447647/151‑1 (MQ=255)
                                                                                                      cgGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCgg                                             >  2:8825553/1‑151 (MQ=255)
                                                                                                       ggTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGc                                            <  1:6174646/151‑1 (MQ=255)
                                                                                                           ggCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAgg                                        >  1:1267153/1‑151 (MQ=255)
                                                                                                            gCGAACTGCCCTTGGCGGAACGCCCGCAGGCACCGAACCGCTTCTATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAggg                                       <  2:11612146/151‑1 (MQ=255)
                                                                                                              gAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGcc                                     >  1:759410/1‑151 (MQ=255)
                                                                                                               aaCTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCa                                    >  2:203796/1‑151 (MQ=255)
                                                                                                                 cTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATc                                  >  1:4759323/1‑151 (MQ=255)
                                                                                                                  tGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGGTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCt                                 >  1:9818788/1‑151 (MQ=255)
                                                                                                                    ccATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCTGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACAGGCTCGACTGGTGCTCCGGCAAGGGCCATCTgg                               <  2:2654249/151‑1 (MQ=255)
                                                                                                                    ccATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCTgg                               >  2:9772654/1‑151 (MQ=255)
                                                                                                                       ttGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCTGGgcc                            >  2:10621753/1‑151 (MQ=255)
                                                                                                                         ggCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCTGGgccgc                          >  2:178025/1‑151 (MQ=255)
                                                                                                                             gAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCTGGGCCGCctgc                      <  2:11356123/151‑1 (MQ=255)
                                                                                                                              aaCGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCTGGGCCGCctgct                     >  2:2384363/1‑151 (MQ=255)
                                                                                                                                  cccGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCTGGGCCGCCTGCTGGcc                 <  2:4169511/151‑1 (MQ=255)
                                                                                                                                   ccGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCTGGGCCGCCTGCTGGccc                <  2:3694359/151‑1 (MQ=255)
                                                                                                                                    cGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCTGGGCCGCCTGCTGGCCCa               >  1:533035/1‑151 (MQ=255)
                                                                                                                                      cAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCTGGGCCGCCTGCTGGCCCATg             <  2:9683096/151‑1 (MQ=255)
                                                                                                                                       aGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCTGGGCCGCCTGCTGGCCCATGc            <  2:6819591/151‑1 (MQ=255)
                                                                                                                                       aGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCTGGGCCGCCTGCTGGCCCATGc            <  1:6520022/151‑1 (MQ=255)
                                                                                                                                         gCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCCAGGGCCATCTGGGCCGCCTGCTGGCCCATGCgg          >  2:4531772/1‑151 (MQ=255)
                                                                                                                                          cACCGAACCGCTTCAATGTCGACGGACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCTGGGCCGCCTGCTGGCCCATGCggg         <  1:8229376/151‑1 (MQ=255)
                                                                                                                                           agcGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGGTCCGGCAAGGGCCATCTGGGCCGCCTGCTGGCCCATGCgggg        <  1:11467581/149‑1 (MQ=255)
                                                                                                                                           aCCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCTGGGCCGCCTGCTGGCCCATGCgggg        >  2:6122/1‑151 (MQ=255)
                                                                                                                                           aCCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCTGGGCCGCCTGCTGGCCCATGCgggg        <  1:6646708/151‑1 (MQ=255)
                                                                                                                                             cGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCTGGGCCGCCTGCTGGCCCATGCGGGGCa      >  2:3281351/1‑151 (MQ=255)
                                                                                                                                             cGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCTGGGCCGCCTGCTGGCCCATGCGGGGCa      <  2:11005959/151‑1 (MQ=255)
                                                                                                                                             cGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCTGGGCCGCCTGCTGGCCCATGCGGGGCa      >  1:2709207/1‑151 (MQ=255)
                                                                                                                                               aaCCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCTGGGCCGCCTGCTGGCCCATGCGGGGCAAc    >  2:8530097/1‑151 (MQ=255)
                                                                                                                                                aCCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCTGGGCCGCCTGCTGGCCCATGCGGGGCAAcc   <  1:4488390/151‑1 (MQ=255)
                                                                                                                                                aCCGCTTCAATGTCGACGGACCGGGACGCAAGTGGCAGCAGATCGGCCTGGTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCTGGGCCGCCTGCTGGCCCATGCGGGGCAAcc   <  2:5145870/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GGCGCAGCCTCGAACAGGCCGACGCGGAGCACAACCAGCCCACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGGTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCTGGGCCGCCTGCTGGCCCATGCGGGGCAACCG  >  NC_008463/6075918‑6076213

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑