Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 72,440 T→C K132E (AAG→GAG)  PA14_RS00315 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00846372,4400TC99.4% 2027.2 / ‑4.4 516K132E (AAG→GAG) PA14_RS00315hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (195/317);  minor base A (0/3);  total (196/320)
Fisher's exact test for biased strand distribution p-value = 2.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 520 total aligned reads displayed.

CGGCAGCGGTCCGGCGCAGCCGGCGAGGAGCAGCAGCGCGAGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTTGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGAACTTGAGGTTGAGCTGGCAATCGCGCCAGAGCGGGCT  >  NC_008463/72292‑72587
                                                                                                                                                    |                                                                                                                                                   
cGGCAGCGGTCGGGCGCAGCCGGCGAGGAGCTGCAGCGCGAGCGGGACGAGACGGGTGAGTGGACGCTTGGGGCGGGCTCGGGTGGGCGTTCAGCTCCTAGAATAGCGCCCTCGCCTTGGGCGGTTCAGACCTTCGCGCAGCGCCCCTCGc                                                                                                                                                   <  1:10021277/151‑1 (MQ=255)
  gCAGCGGTCCGGCGCAGCCGGCGAGGAGCAGCAGCGCGAGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCgg                                                                                                                                                 >  1:10517283/1‑151 (MQ=255)
    aGCGGTCCGGCGCAGCCGGCGAGGAGCAGCAGCGCGAGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGcc                                                                                                                                               <  2:5935350/151‑1 (MQ=255)
    aGCGGTCCGGCGCAGCCGGCGAGGAGCAGCAGCGCGAGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGcc                                                                                                                                               >  1:3768396/1‑151 (MQ=255)
    aGCGGTCCGGCGCAGCCGGCGAGGAGCAGCAGCGCGAGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGcc                                                                                                                                               <  2:4592953/151‑1 (MQ=255)
       ggTCCGGCGCAGCCGGCGAGGAGCAGCAGCGCGAGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCcgcg                                                                                                                                            >  1:4084170/1‑151 (MQ=255)
       ggTCCGGCGCAGCCGGCGAGGAGCAGCAGCGCGAGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCcgcg                                                                                                                                            >  2:10048712/1‑151 (MQ=255)
          ccGGCGCAGCCGGCGAGGAGCAGCAGCGCGAGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCTTAGCGCCCGCGCCTTGCGCCGTTCAGTCCGTCGCGCAGCGCCCCTCGCGGCCgcgcgc                                                                                                                                         >  2:6751348/1‑151 (MQ=255)
          ccGGCGCAGCCGGCGAGGAGCAGCAGCGCGAGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCgcgcgc                                                                                                                                         >  2:2856452/1‑151 (MQ=255)
               gcAGCCGGCGAGGAGCAGCAGCGCGAGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAAtt                                                                                                                                    <  1:9766707/151‑1 (MQ=255)
               gcAGCCGGCGAGGAGCAGCAGCGCGAGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAAtt                                                                                                                                    <  2:4285996/151‑1 (MQ=255)
                cAGCCGGCGAGGAGCAGCAGCGCGAGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTc                                                                                                                                   >  1:2646237/1‑151 (MQ=255)
                    cGGCGAGGAGCAGCAGCGCGAGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCtg                                                                                                                               <  2:8814940/151‑1 (MQ=255)
                       cGAGGAGCAGCAGCGCGAGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCtgttg                                                                                                                            >  1:8931976/1‑151 (MQ=255)
                        gaggagCAGCAGCGCGAGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCtgttgt                                                                                                                           <  2:801560/151‑1 (MQ=255)
                        gaggagCAGCAGCGCGAGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTAAGCTCCTAGCATAGCGCACTCGCCTTGCGACGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCGtgttgt                                                                                                                           >  2:299380/1‑151 (MQ=255)
                          ggagCAGCAGCGCGAGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTcc                                                                                                                         <  1:9933356/151‑1 (MQ=255)
                           gagCAGCAGCGCGAGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCt                                                                                                                        >  2:4865322/1‑151 (MQ=255)
                             gcagcagcGCGAGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTgg                                                                                                                      >  1:5405285/1‑151 (MQ=255)
                               agcagcGCGAGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTc                                                                                                                    >  1:6265540/1‑151 (MQ=255)
                                     gcgAGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTagag                                                                                                              <  2:9106610/151‑1 (MQ=255)
                                        aGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCtt                                                                                                           <  2:7219129/151‑1 (MQ=255)
                                        aGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCtt                                                                                                           <  2:11273790/151‑1 (MQ=255)
                                         gCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTc                                                                                                          >  1:7930496/1‑151 (MQ=255)
                                         gCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTc                                                                                                          <  2:5000552/151‑1 (MQ=255)
                                         gCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTc                                                                                                          <  1:2964131/151‑1 (MQ=255)
                                           gggACGAGACGGGTGGGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGc                                                                                                        >  1:2531316/1‑151 (MQ=255)
                                           gggACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGc                                                                                                        >  1:9895420/1‑151 (MQ=255)
                                           gggACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGc                                                                                                        <  2:9321442/151‑1 (MQ=255)
                                             gACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGccc                                                                                                      >  2:81612/1‑151 (MQ=255)
                                              aCGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCa                                                                                                     <  2:5805402/151‑1 (MQ=255)
                                               cGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAg                                                                                                    <  1:9179165/151‑1 (MQ=255)
                                                gagaCGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAgg                                                                                                   <  2:8357385/151‑1 (MQ=255)
                                                 agaCGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAggg                                                                                                  <  1:7287910/151‑1 (MQ=255)
                                                       gTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGaa                                                                                            <  1:8458731/151‑1 (MQ=255)
                                                        tGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAg                                                                                           >  1:3378716/1‑151 (MQ=255)
                                                        tGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCACCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAg                                                                                           <  2:6587092/151‑1 (MQ=255)
                                                            tGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGa                                                                                       <  2:2639475/151‑1 (MQ=255)
                                                             ggACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGAt                                                                                      <  2:11078876/151‑1 (MQ=255)
                                                               aCGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCACTTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGAtgt                                                                                    <  1:5049654/151‑1 (MQ=255)
                                                                cGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGAtgtg                                                                                   <  1:11621871/151‑1 (MQ=255)
                                                                 gCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGAGGTGc                                                                                  >  2:1353692/1‑151 (MQ=255)
                                                                   aTGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGccc                                                                                >  2:6136760/1‑151 (MQ=255)
                                                                    tGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCg                                                                               <  2:2793978/151‑1 (MQ=255)
                                                                     ggggCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCgg                                                                              <  2:6495599/151‑1 (MQ=255)
                                                                       ggcgggcTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGcc                                                                            <  1:10923000/151‑1 (MQ=255)
                                                                       ggcgggcTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGcc                                                                            <  1:616702/151‑1 (MQ=255)
                                                                        gcgggcTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCt                                                                           <  1:7553088/151‑1 (MQ=255)
                                                                        gcgggcTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCt                                                                           <  2:4384223/151‑1 (MQ=255)
                                                                            gcTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAg                                                                       >  2:3182117/1‑151 (MQ=255)
                                                                             cTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCGTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAgg                                                                      <  2:7092765/151‑1 (MQ=255)
                                                                             cTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAgg                                                                      >  1:7382186/1‑151 (MQ=255)
                                                                               ccGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCg                                                                    >  1:9614197/1‑151 (MQ=255)
                                                                                 ggAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGt                                                                  <  1:1143574/151‑1 (MQ=255)
                                                                                   aGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAg                                                                <  1:4781598/151‑1 (MQ=255)
                                                                                   aGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAg                                                                <  2:1742535/151‑1 (MQ=255)
                                                                                    gggCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCAGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAgc                                                               <  2:211184/151‑1 (MQ=255)
                                                                                      gCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAgcgc                                                             <  2:7946078/151‑1 (MQ=255)
                                                                                      gCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAgcgc                                                             <  2:1016380/151‑1 (MQ=255)
                                                                                       cGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCt                                                            <  1:2167330/151‑1 (MQ=255)
                                                                                        gTTCAGCTCCTAGCATCGCGCCCTCGCCTTGCGCCGTTCAGTCCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTg                                                           <  2:3684504/151‑1 (MQ=255)
                                                                                        gTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTg                                                           <  1:6645775/151‑1 (MQ=255)
                                                                                           cAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGcc                                                        <  1:1128614/151‑1 (MQ=255)
                                                                                            aGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCACTTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCg                                                       <  1:7146779/151‑1 (MQ=255)
                                                                                             gCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCgg                                                      >  1:11128728/1‑151 (MQ=255)
                                                                                               tCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGgcg                                                    >  1:1580377/1‑151 (MQ=255)
                                                                                                 cTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCt                                                  >  2:393886/1‑151 (MQ=255)
                                                                                                 cTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCt                                                  >  1:10298215/1‑151 (MQ=255)
                                                                                                    gCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGaa                                               >  1:2009425/1‑151 (MQ=255)
                                                                                                       tAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCTCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTc                                            >  1:10130184/1‑151 (MQ=255)
                                                                                                        aGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTcc                                           <  2:10874904/151‑1 (MQ=255)
                                                                                                        aGCGCCCACGCCGTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACTGAAGCCGATGTGCCCGGCCTCCTGGCGGTAGCGCTGGCCGGCGCTGAAGTcc                                           <  2:551485/151‑1 (MQ=255)
                                                                                                         gcgcCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCt                                          >  1:4570405/1‑151 (MQ=255)
                                                                                                            cccTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGa                                       <  1:5788895/151‑1 (MQ=255)
                                                                                                                cGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGAACtt                                   <  2:1593068/151‑1 (MQ=255)
                                                                                                                cGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGAACtt                                   >  1:8043030/1‑151 (MQ=255)
                                                                                                                  ccTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTCGCGCTGGCCGGCGCTGAAGTCCTTGAACTTGa                                 >  1:564331/1‑151 (MQ=255)
                                                                                                                    ttGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGAACTTGAgg                               <  1:1078013/151‑1 (MQ=255)
                                                                                                                    ttGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGAACTTGAgg                               <  1:6876247/151‑1 (MQ=255)
                                                                                                                      gcgcCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAAGCCCCCGTTGGCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGCAGTCCTTGAACTTGAGGtt                             <  2:9756487/151‑1 (MQ=255)
                                                                                                                           gTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGAACTTGAGGTTGAGCt                        <  2:7639816/151‑1 (MQ=255)
                                                                                                                           gTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGAACTTGAGGTTGAGCt                        >  1:8091891/1‑151 (MQ=255)
                                                                                                                           gTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGAACTTGAGGTTGAGCt                        >  2:3529792/1‑151 (MQ=255)
                                                                                                                              cAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGAACTTGAGGTTGAGCTGGc                     <  1:3472921/151‑1 (MQ=255)
                                                                                                                               aGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTACGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTACCGCTGGCCGGCGCTGAAGTCCTTGAACTTGAGGTTGAGCTGGCa                    <  1:649676/151‑1 (MQ=255)
                                                                                                                                gACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGAACTTGAGGTTGAGCTGGCaa                   <  2:5300791/151‑1 (MQ=255)
                                                                                                                                 aCCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGAACTTGAGGTTGAGCTGGCAAt                  <  1:6959710/151‑1 (MQ=255)
                                                                                                                                 aCCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTCGAACTTGAGGTTGAGCTGGCAAt                  <  2:19160/151‑1 (MQ=255)
                                                                                                                                       gcgcAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGAACTTGAGGTTGAGCTGGCAATCGCGcc            >  1:1748222/1‑151 (MQ=255)
                                                                                                                                        cgcAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGAACTTGAGGTTGAGCTGGCAATCGCGCCa           <  1:10443269/151‑1 (MQ=255)
                                                                                                                                          cAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGAACTTGAGGTTGAGCTGGCAATCGCGCCaga         <  2:10554722/151‑1 (MQ=255)
                                                                                                                                          cAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGAACTTGAGGTTGAGCTGGCAATCGCGCCaga         <  2:9033497/151‑1 (MQ=255)
                                                                                                                                          cAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGAACTTGAGGTTGAGCTGGCAATCGCGCCaga         <  2:2465191/151‑1 (MQ=255)
                                                                                                                                           aGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGAACTTGAGGTTGAGCTGGCAATCGCGCCagag        <  1:4265001/151‑1 (MQ=255)
                                                                                                                                           aGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCGTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGAACTTGAGGTTGAGCTGGCAATCGCGCCagag        <  2:11592503/151‑1 (MQ=255)
                                                                                                                                             cgcCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGAACTTGAGGTTGAGCTGGCAATCGCGCCAGAGCg      <  1:2840450/151‑1 (MQ=255)
                                                                                                                                             cgcCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCACCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGAACTTGAGGTTGAGCTGGCAATCGCGCCAGAGCg      <  2:9603875/151‑1 (MQ=255)
                                                                                                                                              gcCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGAACTTGAGGTTGAGCTGGCAATCGCGCCAGAGCgg     >  2:7436303/1‑151 (MQ=255)
                                                                                                                                              gcCCCTCGCGGCCGCGCGCCAAGTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGAACTTGAGGTTGAGCTGGCAATCGCGCCAGAGCgg     <  2:2201189/151‑1 (MQ=255)
                                                                                                                                                cccTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGAACTTGAGGTTGAGCTGGCAATCGCGCCAGAGCGGGc   <  1:8733707/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CGGCAGCGGTCCGGCGCAGCCGGCGAGGAGCAGCAGCGCGAGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTTGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGAACTTGAGGTTGAGCTGGCAATCGCGCCAGAGCGGGCT  >  NC_008463/72292‑72587

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑