Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 1,128,162 N→C intergenic (+23/‑38) PA14_RS05280 → / → PA14_RS05285 DUF1302 domain‑containing protein/DUF1329 domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084631,128,1620NC99.8% 1747.2 / NA 464intergenic (+23/‑38)PA14_RS05280/PA14_RS05285DUF1302 domain‑containing protein/DUF1329 domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base N (0/0);  major base C (238/224);  minor base A (1/0);  total (239/225)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 469 total aligned reads displayed.

GGGGGTGGACGCGGAGTACCAGAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTNNNANCNGGNCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAGGCGGCCAAGCTCGGCACCAGCC  >  NC_008463/1128013‑1128311
                                                                                                                                                     |                                                                                                                                                     
gggggTGGACGCGGAGTACCAGAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCg                                                                                                                                                      <  2:2300764/151‑1 (MQ=255)
gggggTGGACGCGGAGTACCAGAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCg                                                                                                                                                      <  2:105210/151‑1 (MQ=255)
gggggTGGACGCGGAGTACCAGAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCg                                                                                                                                                      <  2:9430807/151‑1 (MQ=255)
   ggTGGACGCGGAGTACCAGAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGc                                                                                                                                                   <  2:1767447/151‑1 (MQ=255)
   ggTGGACGCGGAGTACCAGAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGc                                                                                                                                                   <  2:4520613/151‑1 (MQ=255)
   ggTGGACGCGGAGTACCAGAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGc                                                                                                                                                   <  1:1128878/151‑1 (MQ=255)
      ggACGCGGAGTACCAGAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCgg                                                                                                                                                >  2:4964964/1‑151 (MQ=255)
       ggcgcgGAGTACCAGAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGAGCGCGACTTCGTCGCGCTCAGCGTCGGCATGTACTTGTGTTGTACGGCGGCGCGGTGCGGACCGGGCCggg                                                                                                                                               >  1:2906834/3‑150 (MQ=255)
            ggAGTACCAGAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCa                                                                                                                                          <  1:5129251/151‑1 (MQ=255)
             gAGTACCATAACACCTCCACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAg                                                                                                                                         <  1:4195947/151‑1 (MQ=255)
              aGTACCAGAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGAAGGCAAGTACACCACCGTGGACGATCGCGACTTCGTAGCGCTCACCATCGGCATGAACTTCGGAATTAAGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAgg                                                                                                                                        >  2:2928921/1‑151 (MQ=255)
                tACCAGAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGAc                                                                                                                                      >  2:4125785/1‑151 (MQ=255)
                  ccAGAACACCTACACCGCGAGCCTCTCCTACACCACCTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACtt                                                                                                                                    <  2:7678114/151‑1 (MQ=255)
                  ccAGAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACtt                                                                                                                                    >  2:6497378/1‑151 (MQ=255)
                  ccAGAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTATGATGTTCGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACtt                                                                                                                                    >  1:7713763/1‑151 (MQ=255)
                       acacCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGa                                                                                                                               >  2:6725882/1‑151 (MQ=255)
                       acacCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCACAGCATCGGCAAGAACTTCTGATGTACGCCGGCCCCGTGCGAACCGGGCCGGCGAGCAGGACTTAACGa                                                                                                                               >  2:8557174/1‑151 (MQ=255)
                         acCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACATCGTCGCGCTCAGCTTCGGCATGAACTTCAGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAgg                                                                                                                             >  1:2657667/1‑151 (MQ=255)
                          ccTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGAGCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGga                                                                                                                            >  1:6712422/1‑151 (MQ=255)
                           cTACCCCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGAACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGAGTTACGCCGGCCCGGTGCGAACGGGGCCGGCGAGCAGGACTTAACGAGgac                                                                                                                           <  1:5802074/151‑1 (MQ=255)
                           cTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGgac                                                                                                                           >  1:10584432/1‑151 (MQ=255)
                           cTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGgac                                                                                                                           <  2:10012438/151‑1 (MQ=255)
                              cacCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCCTGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGgacgac                                                                                                                        >  2:7508034/1‑151 (MQ=255)
                              cacCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGgacgac                                                                                                                        <  2:10825026/151‑1 (MQ=255)
                               acCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGacgacg                                                                                                                       >  2:7405256/1‑151 (MQ=255)
                                ccGCGAGCCTCTCCTACACCAACTTCTACGACGGCAAGTACACCACCGTGGACCATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGAACGCCGGCCCGCTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGAcgacga                                                                                                                      <  1:819815/151‑1 (MQ=255)
                                  gcgAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGc                                                                                                                    <  2:10962738/151‑1 (MQ=255)
                                    gAGCCTCTCCTACACCACCTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGa                                                                                                                  <  2:7794814/151‑1 (MQ=255)
                                       ccTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATg                                                                                                               <  1:8938361/151‑1 (MQ=255)
                                            ccTCCACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAaac                                                                                                          <  2:388262/151‑1 (MQ=255)
                                            ccTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAaac                                                                                                          >  2:4220478/1‑151 (MQ=255)
                                             cTACCCCACCTTCCTCGACGGCAAGTACACCACCGTCGACGAACGCGACGACGTCGCGCTCAGCTTCGGCATGAACTTCTGCTGTACGCCGGCCCGGAGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAaaca                                                                                                         <  2:7172558/151‑1 (MQ=255)
                                             cTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAaaca                                                                                                         <  2:1367481/151‑1 (MQ=255)
                                              tacccaaaCTTCTTCGACGGCACGTACCCCACCGTGGACGAGCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAaacaa                                                                                                        <  1:1015305/145‑1 (MQ=255)
                                                 acCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGa                                                                                                     >  2:6146224/1‑151 (MQ=255)
                                                  ccAACTTCTTCGACGGCAAGTACACCACCGTGGACGAGCGCGACTTCGTCGCGCTCAGCGTAGGCATGAACTTCTGATGTACGCCGGCCCCGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGaa                                                                                                    >  1:7978184/1‑151 (MQ=255)
                                                    aaCTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGa                                                                                                  <  2:5848966/151‑1 (MQ=255)
                                                     aCTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGAt                                                                                                 <  2:8763650/151‑1 (MQ=255)
                                                      cttcttCGACGGCAAGTACACCACCGTGGACGATAGCGACATCGTCGCGCTCAGCTTCGGCATGAACTTCTGATTTAAGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGAtt                                                                                                >  1:9212833/1‑151 (MQ=255)
                                                       ttcttcGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGAttc                                                                                               <  2:7232265/151‑1 (MQ=255)
                                                       ttcttcGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGAttc                                                                                               >  2:2726112/1‑151 (MQ=255)
                                                       ttcttcGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTAAGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGAttc                                                                                               >  2:10190577/1‑151 (MQ=255)
                                                         cttcGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGGATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGAttctt                                                                                             >  2:9264211/1‑151 (MQ=255)
                                                         cttcGACGGCAAGTACACCACCGTGGACGATCGCGAATTCGTCGCGCTCAGCTTCGGAATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGAttctt                                                                                             <  1:996565/151‑1 (MQ=255)
                                                                ggCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATa                                                                                      <  1:718843/151‑1 (MQ=255)
                                                                 gCAAGTACACCACCGTGGACGAGCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGTTTCTTCTGCATAc                                                                                     >  2:6571139/1‑151 (MQ=255)
                                                                   aaGTACACCACCGTGGACGATCGCGACTTCGTAGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCCGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGCATGAAAACAACGAAGATTCTTATGCATACCg                                                                                   >  1:10017205/1‑151 (MQ=255)
                                                                    aGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCgg                                                                                  <  1:2412519/151‑1 (MQ=255)
                                                                         accaccGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTAc                                                                             >  2:7939584/1‑151 (MQ=255)
                                                                            accGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAATATTCTTCTGCATACCGGTGTACTgg                                                                          >  2:8690971/1‑151 (MQ=255)
                                                                            accGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTgg                                                                          <  2:8936751/151‑1 (MQ=255)
                                                                              cGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGcc                                                                        <  2:9466006/151‑1 (MQ=255)
                                                                                tGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCt                                                                      <  1:8557960/151‑1 (MQ=255)
                                                                                tGGACGATCGCGACTTCGTCGCGCTCAGCTTAGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACAGGccca                                                                      >  1:6018150/1‑150 (MQ=255)
                                                                                    cGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGc                                                                  >  2:2571814/1‑151 (MQ=255)
                                                                                       tCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTTACGTGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCTATCCGGTGTACTGGCCCTGAGCctg                                                               >  1:2094639/1‑151 (MQ=255)
                                                                                         gcgACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCctgct                                                             >  2:2056342/1‑151 (MQ=255)
                                                                                          cgACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGACCCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGTGCctgcgg                                                            >  1:361849/1‑149 (MQ=255)
                                                                                          cgACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCctgctg                                                            >  2:8392876/1‑151 (MQ=255)
                                                                                            aCTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGc                                                          <  2:9593075/151‑1 (MQ=255)
                                                                                                cgtcgCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCAcc                                                      >  2:2238308/1‑151 (MQ=255)
                                                                                                 gtcgCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCAccc                                                     >  1:10842377/1‑151 (MQ=255)
                                                                                                  tcgCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCa                                                    <  2:9127497/151‑1 (MQ=255)
                                                                                                      gcTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTg                                                <  2:11596391/151‑1 (MQ=255)
                                                                                                       cTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGa                                               >  1:6542581/1‑151 (MQ=255)
                                                                                                         cAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATg                                             <  1:8334809/151‑1 (MQ=255)
                                                                                                           gCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATggc                                           <  1:3717475/151‑1 (MQ=255)
                                                                                                            cTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGACTGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATggcg                                          >  1:6963954/1‑151 (MQ=255)
                                                                                                            cTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATggcg                                          >  2:1536149/1‑151 (MQ=255)
                                                                                                               cGGCATGAACTTCTGCTGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGgcggcg                                       <  1:3437168/151‑1 (MQ=255)
                                                                                                                 gCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGt                                     <  1:11543570/151‑1 (MQ=255)
                                                                                                                 gCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGt                                     >  2:11401061/1‑151 (MQ=255)
                                                                                                                 gCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGt                                     >  2:8115978/1‑151 (MQ=255)
                                                                                                                    tGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATc                                  >  2:11184849/1‑151 (MQ=255)
                                                                                                                       aCTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGAATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATccgc                               >  2:2461226/1‑151 (MQ=255)
                                                                                                                        ctactgctgTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGCATGAAAACACCCAAGATTCTTCTGCATACCGGTGTACGGGCCCTGCGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATccgcc                              <  1:7561296/148‑1 (MQ=255)
                                                                                                                        cTTCTGATGTACGCCTTCCCGGTGCGACCCGTGCGGGAGTGCAGGAATTATCGTGGAATTCGTGAGTATGAAAACAACGAAGATTCTTCTTCATACCGGTGTTCTGGCCCTGAGCCTGCTGGCCTCCCAGGTGATGGCGGCGTTATccgcc                              <  2:6409200/151‑1 (MQ=255)
                                                                                                                        cTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATccgcc                              >  2:2471419/1‑151 (MQ=255)
                                                                                                                        cTTCTGATGTACGCCGGCCCGGTGCGAACAGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATccgcc                              >  1:10981993/1‑151 (MQ=255)
                                                                                                                         ttCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATccgccg                             >  1:1518083/1‑151 (MQ=255)
                                                                                                                         ttCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATccgccg                             >  2:10557215/1‑151 (MQ=255)
                                                                                                                            tGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCcgacg                          <  1:4576436/151‑1 (MQ=255)
                                                                                                                              aTGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAg                        <  1:470379/151‑1 (MQ=255)
                                                                                                                                 tACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAggcg                     <  1:3946329/151‑1 (MQ=255)
                                                                                                                                  aCGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAggcgg                    <  2:6230660/151‑1 (MQ=255)
                                                                                                                                     ccGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAGGCGGCCa                 >  1:6337576/1‑151 (MQ=255)
                                                                                                                                      cGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGCGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAGGCGGCCaa                <  2:4392585/151‑1 (MQ=255)
                                                                                                                                      cGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAGGCGGCCaa                >  1:4276807/1‑151 (MQ=255)
                                                                                                                                      cGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAGGCGGCCaa                <  2:4785947/151‑1 (MQ=255)
                                                                                                                                      cGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAGGCGGCCaa                >  2:11047362/1‑151 (MQ=255)
                                                                                                                                       ggCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAGGCGGCCAAg               >  2:10342922/1‑151 (MQ=255)
                                                                                                                                        gCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAGGCGGCCAAGc              >  2:5160054/1‑151 (MQ=255)
                                                                                                                                           cGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACACCGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAGGCGGCCAAGCTCg           <  2:3109144/151‑1 (MQ=255)
                                                                                                                                           cGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAGGCGGCCAAGCTCg           <  1:314080/151‑1 (MQ=255)
                                                                                                                                           cGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAGGCGGCCAAGCTCg           <  1:9732593/151‑1 (MQ=255)
                                                                                                                                            ggTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAGGCGGCCAAGCTCgg          <  1:3314916/151‑1 (MQ=255)
                                                                                                                                            ggTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAGGCGGCCAAGCTCgg          <  2:3240546/151‑1 (MQ=255)
                                                                                                                                              tGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAGGCGGCCAAGCTCGGCa        <  1:10387501/151‑1 (MQ=255)
                                                                                                                                                 gaaCCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAGGCGGCCAAGCTCGGCACCa     <  1:5517241/150‑1 (MQ=255)
                                                                                                                                                  aaCCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAGGCGGCCAAGCTCGGCACCAg    >  2:9057557/1‑151 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GGGGGTGGACGCGGAGTACCAGAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTNNNANCNGGNCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAGGCGGCCAAGCTCGGCACCAGCC  >  NC_008463/1128013‑1128311

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑