BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_008463	2,730,681	2 bp→CG	intergenic (+19/+95)	PA14_RS12860 → / ← ctpH	anti‑virulence regulator CigR family protein/methyl‑accepting chemotaxis protein CtpH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_008463	2,730,681	0	G	C	100.0%	1459.2 / NA	371	intergenic (+19/+96)	PA14_RS12860/ctpH	anti‑virulence regulator CigR family protein/methyl‑accepting chemotaxis protein CtpH
Reads supporting (aligned to +/- strand): ref base G (0/0); new base C (169/202); total (169/202)
*	NC_008463	2,730,682	0	C	G	99.7%	1444.5 / NA	371	intergenic (+20/+95)	PA14_RS12860/ctpH	anti‑virulence regulator CigR family protein/methyl‑accepting chemotaxis protein CtpH
Reads supporting (aligned to +/- strand): ref base C (0/0); major base G (168/202); minor base T (1/0); total (169/202)
Fisher's exact test for biased strand distribution p-value = 4.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 371 total aligned reads displayed.

CGGCCGCCTGCTAGGCCGGCTGCCGCACTACGACGGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGCAGGCCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGCAGGCGTTGCAGGGCATCGCCCAGTTGCGCGGAGAG  >  NC_008463/2730536‑2730821

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cGGCCGCCTGCTAGGCCGGCTGCCGCACTACGACGGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCAt                                                                                                                                         >  1:4631343/1‑151 (MQ=255)

 ggCCGCCTGCTAGGCCGGCTGCCGCACTACGACGGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATa                                                                                                                                        >  2:11342908/1‑151 (MQ=255)

   ccgccTGCTAGGCCGGCTGCCGCACTACGACGGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAgg                                                                                                                                      <  1:6553191/151‑1 (MQ=255)

   ccgccTGCTAGGCCGGCTGCCGCACTACGACGGCTACGAGTGGAGGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAgg                                                                                                                                      <  2:8414076/151‑1 (MQ=255)

    cgccTGCTAGGCCGGCTGCCGCACAACGACGGCTACGAGTGGATGCAGGCCGGCACCGTCCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGgc                                                                                                                                     <  2:7851760/151‑1 (MQ=255)

     gccTGCTAGGCCGGCTGCCGCACTACGACGGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGgcg                                                                                                                                    <  1:6980103/151‑1 (MQ=255)

     gccTGCTAGGCCGGCTGCCGCACTACGACGGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGgcg                                                                                                                                    >  1:2243690/1‑151 (MQ=255)

      ccTGCTAGGCCGGCTGCCGCACTACGACGGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGgcgc                                                                                                                                   >  1:8380402/1‑151 (MQ=255)

       cTGCTAGGCCGGCTGCCGCACTACGACGGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCt                                                                                                                                  <  1:9725626/151‑1 (MQ=255)

        tGCTAGGCCGGCTGCCGCACTACGACGGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTa                                                                                                                                 <  1:7507622/151‑1 (MQ=255)

          cTAGGCCGGCTGCCGCACTACGACGGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATc                                                                                                                               <  2:252953/151‑1 (MQ=255)

           tAGGCCGGCTGCCGCACTACGACGGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATcc                                                                                                                              <  1:1212681/151‑1 (MQ=255)

            aGGCCGGCTGCCGCACTACGACGGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCg                                                                                                                             <  1:10284749/151‑1 (MQ=255)

             ggCCGGCTGCCGCACTACGACGGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACGGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGc                                                                                                                            >  2:1708059/1‑151 (MQ=255)

              gCCGGCTGCCGCACTACGACGGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGcc                                                                                                                           <  2:10824228/151‑1 (MQ=255)

                  gCTGCCGCACTACGACGGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGc                                                                                                                       <  2:6764073/151‑1 (MQ=255)

                  gCTGCCGCACTACGACGGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGc                                                                                                                       <  2:811371/151‑1 (MQ=255)

                   cTGCCGCACTACGACGGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGcc                                                                                                                      <  1:811202/151‑1 (MQ=255)

                     gCCGCACTACGACGGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGAGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCTCCATAGGCGCTATCCGCCAGGcccc                                                                                                                    >  2:5742170/1‑151 (MQ=255)

                         cACTACGACGGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTg                                                                                                                <  1:3567144/151‑1 (MQ=255)

                          aCTACGACGGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTgg                                                                                                               >  2:4341985/1‑151 (MQ=255)

                          aCTACGACGGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGGTgg                                                                                                               >  1:9912369/1‑151 (MQ=255)

                          aCTACGACGGCTACGAGTGGATGCAGGCCGGCACCGACCTGCGGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTgg                                                                                                               <  2:6050737/151‑1 (MQ=255)

                             acgacgGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACACCGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGt                                                                                                            <  1:4311578/151‑1 (MQ=255)

                              cgacgGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTg                                                                                                           <  2:5032339/151‑1 (MQ=255)

                              cgacgGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTg                                                                                                           >  2:6317443/1‑151 (MQ=255)

                               gccgGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTgg                                                                                                          <  2:2989153/149‑1 (MQ=255)

                                acgGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGc                                                                                                         <  1:877004/151‑1 (MQ=255)

                                 cgGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCg                                                                                                        <  2:9732301/151‑1 (MQ=255)

                                  ggCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCgg                                                                                                       >  1:10748857/1‑151 (MQ=255)

                                    cTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGAt                                                                                                     <  1:537737/151‑1 (MQ=255)

                                     tACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATa                                                                                                    <  2:2539997/151‑1 (MQ=255)

                                      aCGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGCGCGGGCCAGGGCGGGTAGCGCAATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATaa                                                                                                   <  1:8706343/151‑1 (MQ=255)

                                      aCGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATaa                                                                                                   <  2:284540/151‑1 (MQ=255)

                                       cGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAAc                                                                                                  >  1:7755975/1‑151 (MQ=255)

                                        gAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAAcc                                                                                                 <  2:7709697/151‑1 (MQ=255)

                                           tGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCgtg                                                                                              >  2:11034165/1‑151 (MQ=255)

                                            ggATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCgtgt                                                                                             >  1:10848381/1‑151 (MQ=255)

                                              ctGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCTGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTg                                                                                           <  1:3075119/150‑1 (MQ=255)

                                              aggCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGACGTGCTGGACAACGCACTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTg                                                                                           <  2:4039/149‑1 (MQ=255)

                                               tGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTgc                                                                                          >  1:8878384/1‑151 (MQ=255)

                                                  aGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTTGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCt                                                                                       <  2:4672959/151‑1 (MQ=255)

                                                  aGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTgcgcg                                                                                       >  2:6787887/1‑150 (MQ=255)

                                                   ggCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCtt                                                                                      <  1:8552604/151‑1 (MQ=255)

                                                     ccGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGt                                                                                    <  1:7393284/151‑1 (MQ=255)

                                                       ggCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGACGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTcc                                                                                  <  2:8721384/151‑1 (MQ=255)

                                                         cACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGc                                                                                <  2:511549/151‑1 (MQ=255)

                                                          aCCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGCGTGGCGGATAACCGTGTTGCGCTTGTCCGcc                                                                               >  2:7429530/1‑151 (MQ=255)

                                                          aCCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGcc                                                                               >  2:2329389/1‑151 (MQ=255)

                                                           ccGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGccc                                                                              >  2:9263967/1‑151 (MQ=255)

                                                            cGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCt                                                                             >  1:6738618/1‑151 (MQ=255)

                                                            cGACCTGCGGCTGGTGGCGGTGGCGACCGGGCTGATCTACGACGTGCTGGACAACGCTCCCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCt                                                                             <  1:7217531/151‑1 (MQ=255)

                                                               ccTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTccc                                                                          >  2:4128546/1‑151 (MQ=255)

                                                               ccTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTAcc                                                                          <  1:10173141/151‑1 (MQ=255)

                                                                ctgctgctgGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCt                                                                         >  1:273310/1‑151 (MQ=255)

                                                                    tgctgGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTg                                                                     >  2:8974516/1‑151 (MQ=255)

                                                                     gctgGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTgg                                                                    <  2:4565709/151‑1 (MQ=255)

                                                                        ggtggcggtggcGACCGGGCTGTTCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAg                                                                 >  2:11603476/1‑151 (MQ=255)

                                                                        ggtggcggtggcGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAg                                                                 <  1:2573434/151‑1 (MQ=255)

                                                                        ggtggcggtggcGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAg                                                                 <  1:11569687/151‑1 (MQ=255)

                                                                          tggcggtggcgACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTAGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTc                                                               >  2:3277283/1‑151 (MQ=255)

                                                                            gcggtggcgACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCt                                                             <  2:3682333/151‑1 (MQ=255)

                                                                             cggtggcgACCGGGCTGATCTACGACGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCtt                                                            <  2:147261/151‑1 (MQ=255)

                                                                             cggtggcgACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCtt                                                            <  1:1906595/151‑1 (MQ=255)

                                                                               gtggcgACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCtttt                                                          <  1:9694888/151‑1 (MQ=255)

                                                                                 ggcgACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCt                                                        <  2:5549696/151‑1 (MQ=255)

                                                                                   cgACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTa                                                      <  1:9238718/151‑1 (MQ=255)

                                                                                    gACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTAc                                                     >  2:10478881/1‑151 (MQ=255)

                                                                                    gACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTAc                                                     <  2:3182692/151‑1 (MQ=255)

                                                                                    gACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTAc                                                     >  1:9208848/1‑151 (MQ=255)

                                                                                        gggCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCa                                                 <  1:2402914/151‑1 (MQ=255)

                                                                                          gCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAgg                                               <  1:1418789/151‑1 (MQ=255)

                                                                                            tGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTc                                             >  2:7208938/1‑151 (MQ=255)

                                                                                             gATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCa                                            <  1:2615806/151‑1 (MQ=255)

                                                                                             gATCAACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAAACGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCa                                            >  2:10321866/1‑151 (MQ=255)

                                                                                              aTCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAg                                           >  1:7902410/1‑151 (MQ=255)

                                                                                              aTCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAg                                           <  1:3980745/151‑1 (MQ=255)

                                                                                               tCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAgg                                          >  1:610368/1‑151 (MQ=255)

                                                                                                cTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGc                                         >  1:1677224/1‑151 (MQ=255)

                                                                                                       gTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGCAgg                                  >  1:11456424/1‑151 (MQ=255)

                                                                                                         gCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGCAGGCg                                <  2:9804152/151‑1 (MQ=255)

                                                                                                         gCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGCAGGCg                                <  1:8638625/151‑1 (MQ=255)

                                                                                                         gCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGCAGGCg                                >  1:381732/1‑151 (MQ=255)

                                                                                                           tGGACAACGCACTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGCAGGCGtt                              <  1:508602/151‑1 (MQ=255)

                                                                                                             gACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGCAGGCGTTGc                            <  1:2156693/151‑1 (MQ=255)

                                                                                                             gACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGCAGGCGTTGc                            >  1:7296604/1‑151 (MQ=255)

                                                                                                             gACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGCAGGCGTTGc                            >  1:473709/1‑151 (MQ=255)

                                                                                                                  cGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGCAGGCGTTGCAGGGc                       >  1:10013290/1‑151 (MQ=255)

                                                                                                                     tctcGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGCAGGCGTTGCAGGGCATc                    <  2:8195686/151‑1 (MQ=255)

                                                                                                                      ctcGACGGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGCAGGCGTTGCAGGGCATCg                   <  2:10250092/151‑1 (MQ=255)

                                                                                                                          aCTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGCAGGCGTTGCAGGGCATCGCCCa               <  2:1911356/151‑1 (MQ=255)

                                                                                                                           cTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGCAGGCGTTGCAGGGCATCGCCCAg              <  2:9539989/151‑1 (MQ=255)

                                                                                                                             gAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCAAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGCAGGCGTTGCAGGGCATCGCCCAGtt            >  1:6214729/1‑151 (MQ=255)

                                                                                                                                cGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCGCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGCTGGCGTTGCAGGGCATCGCCCAGTTgcg         <  1:5722106/151‑1 (MQ=255)

                                                                                                                                 gggCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGCAGGCGTTGCAGGGCATCGCCCAGTTgcgc        <  1:6449508/151‑1 (MQ=255)

                                                                                                                                  ggCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGATGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGCAGGCGTTGCAGGGCATCGCCCAGTTGcgcg       <  1:4180636/151‑1 (MQ=255)

                                                                                                                                   gCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGCAGGCGTTGCAGGGCATCGCCCAGTTGCGCgg      >  2:3820410/1‑151 (MQ=255)

                                                                                                                                    ccAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGCAGGCGTTGCAGGGCATCGCCCAGTTGCGCGga     <  2:5429032/151‑1 (MQ=255)

                                                                                                                                    ccAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGCAGGCGTTGCAGGGCATCGCCCAGTTGCGCGga     <  1:9480090/151‑1 (MQ=255)

                                                                                                                                     cAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGCAGGCGTTGCAGGGCATCGCCCAGTTGCGCGgag    >  1:3292299/1‑151 (MQ=255)

||

CGGCCGCCTGCTAGGCCGGCTGCCGCACTACGACGGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGCAGGCCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGCAGGCGTTGCAGGGCATCGCCCAGTTGCGCGGAGAG  >  NC_008463/2730536‑2730821

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`