Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 155,993 Δ1 bp coding (922/981 nt) PA14_RS00700 → ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_008463155,9900G.100.0% 2750.5 / NA 552coding (919/981 nt)PA14_RS00700ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (136/416);  total (136/416)

Only 100 of 570 total aligned reads displayed.

GTGGCCCAGGGGATCGGGCTGTCGATTCCGGCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCCGGGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCAATGGGGCATTTTTGCCGAACCCCGTTAAGATTCTCCCAGTCGATTTGAAACAC  >  NC_008463/155840‑156136
                                                                                                                                                      |                                                                                                                                                  
gtggCCCAGGGGATCGGGCTGTCGATTCCGGCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCCg                                                                                                                                                    <  1:4134429/151‑1 (MQ=255)
     ccAGGGGATCGGGCTGTCGATTCCGGCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCCgggcac                                                                                                                                               >  2:1510310/1‑148 (MQ=255)
      cAGGGGATCGGGCTGTCGATTCCGGCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCCgggcact                                                                                                                                              >  1:10581574/1‑147 (MQ=255)
         gggATCGGGCTGTCGATTCCGGCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGAc                                                                                                                                          <  1:10381019/151‑1 (MQ=255)
         gggATCGGGCTGTCGATTCCGGCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGAc                                                                                                                                          <  2:7076051/151‑1 (MQ=255)
          ggATCGGGCTGTCGATTCCGGCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACg                                                                                                                                         <  1:1002513/151‑1 (MQ=255)
           gATCGGGCTGTCGATTCCGGCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGa                                                                                                                                        <  2:7856434/151‑1 (MQ=255)
              cGGGCTGTCGATTCCGGCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAggg                                                                                                                                     <  1:6893086/151‑1 (MQ=255)
              cGGGCTGTCGATTCCGGCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAggg                                                                                                                                     <  2:4648853/151‑1 (MQ=255)
               gggCTGTCGATTCCGGCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGc                                                                                                                                    <  1:51409/151‑1 (MQ=255)
                 gCTGTCGATTCCGGCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAg                                                                                                                                  <  1:7602795/151‑1 (MQ=255)
                  cTGTCGATTCCGGCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAgg                                                                                                                                 <  1:7200672/151‑1 (MQ=255)
                    gTCGATTCCGGCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGtt                                                                                                                               <  2:3541754/151‑1 (MQ=255)
                    gTCGATTCCGGCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCGGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGtt                                                                                                                               <  2:5059267/151‑1 (MQ=255)
                      cGATTCCGGCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGttct                                                                                                                             <  1:2923021/151‑1 (MQ=255)
                       gATTCCGGCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGttctt                                                                                                                            <  2:10297021/151‑1 (MQ=255)
                         ttCCGGCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCg                                                                                                                          <  1:6175165/151‑1 (MQ=255)
                           ccGGCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGAt                                                                                                                        <  2:399526/151‑1 (MQ=255)
                            cGGCCATCCTGCTGGCGATGCTGCCCTATGCGGCGACCATGCTGGTGCTGGTCCTGCTCTCGCTGGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTTGAAGGGC‑GGGCACTGACGAGGGCAGGTTCTTCGAtt                                                                                                                       <  2:10640843/151‑1 (MQ=255)
                            cGGCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGAtt                                                                                                                       <  2:6498622/151‑1 (MQ=255)
                             ggCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTa                                                                                                                      <  1:4638193/151‑1 (MQ=255)
                             ggCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTa                                                                                                                      >  2:4557766/1‑151 (MQ=255)
                             ggCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTa                                                                                                                      <  2:8507975/151‑1 (MQ=255)
                              gCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAg                                                                                                                     >  1:8785170/1‑151 (MQ=255)
                              gCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAg                                                                                                                     <  1:4517442/151‑1 (MQ=255)
                               ccAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAgt                                                                                                                    <  2:8339633/151‑1 (MQ=255)
                                cAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAgtt                                                                                                                   <  2:9633797/151‑1 (MQ=255)
                                cAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAgtt                                                                                                                   >  1:7986878/1‑151 (MQ=255)
                                 aaCCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAgttg                                                                                                                  <  1:5835857/151‑1 (MQ=255)
                                 aaCCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAgttg                                                                                                                  <  1:11331559/151‑1 (MQ=255)
                                 aaCCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAgttg                                                                                                                  <  2:143189/151‑1 (MQ=255)
                                   ccTGCTGGCGATGCGGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAgttgtt                                                                                                                <  2:2491970/151‑1 (MQ=255)
                                    ctgctgGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGttt                                                                                                               <  1:10752459/151‑1 (MQ=255)
                                    ctgctgGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGttt                                                                                                               >  1:8398454/1‑151 (MQ=255)
                                      gctgGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTa                                                                                                             <  2:521455/151‑1 (MQ=255)
                                        tgGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAAc                                                                                                           >  1:966743/1‑151 (MQ=255)
                                         ggCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACt                                                                                                          <  1:6355816/151‑1 (MQ=255)
                                         ggCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACt                                                                                                          <  1:11122420/151‑1 (MQ=255)
                                         ggCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACt                                                                                                          <  2:3753782/151‑1 (MQ=255)
                                         ggCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACt                                                                                                          <  1:8184486/151‑1 (MQ=255)
                                              tgctgcCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTta                                                                                                     <  2:7735956/151‑1 (MQ=255)
                                               gctgcCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTtat                                                                                                    <  1:4870088/151‑1 (MQ=255)
                                                  gcCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTtatata                                                                                                 >  2:3358329/1‑151 (MQ=255)
                                                  gcCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTtatata                                                                                                 >  1:6712028/1‑151 (MQ=255)
                                                    ccTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGTGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATAtt                                                                                               >  2:1658374/1‑151 (MQ=255)
                                                     cTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATAttc                                                                                              <  1:8292870/151‑1 (MQ=255)
                                                      tATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATAttct                                                                                             <  1:5410810/151‑1 (MQ=255)
                                                      tATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATAttct                                                                                             <  2:8853177/151‑1 (MQ=255)
                                                        tGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATAttcttc                                                                                           <  2:10277552/151‑1 (MQ=255)
                                                        tGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATAttcttc                                                                                           <  2:5452889/151‑1 (MQ=255)
                                                        tGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATAttcttc                                                                                           <  2:6865592/151‑1 (MQ=255)
                                                             cgACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAg                                                                                      >  2:8210368/1‑151 (MQ=255)
                                                             cgACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAg                                                                                      <  2:4389376/151‑1 (MQ=255)
                                                              gACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGa                                                                                     <  1:5622028/151‑1 (MQ=255)
                                                              gACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGa                                                                                     <  2:1990213/151‑1 (MQ=255)
                                                               aCCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTGATATATTCTTCGATAGAt                                                                                    <  2:10568400/151‑1 (MQ=255)
                                                                  aTCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTa                                                                                 <  2:11293370/151‑1 (MQ=255)
                                                                  aTCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTa                                                                                 >  2:363459/1‑151 (MQ=255)
                                                                   tCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAg                                                                                <  1:4439156/151‑1 (MQ=255)
                                                                    ccTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGa                                                                               >  1:2543300/1‑151 (MQ=255)
                                                                     cTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGAt                                                                              <  2:318751/151‑1 (MQ=255)
                                                                       ggTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATat                                                                            <  1:2765661/151‑2 (MQ=255)
                                                                        gTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAAt                                                                           <  1:3033581/151‑1 (MQ=255)
                                                                         tGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAAtt                                                                          <  2:74541/151‑1 (MQ=255)
                                                                          gCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAAttt                                                                         <  1:1812336/151‑1 (MQ=255)
                                                                           cTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTc                                                                        >  1:331846/1‑151 (MQ=255)
                                                                               tCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGATTTAACTATTTATATATACTTCGATAGATTTAGATAATATCACTg                                                                    >  2:1245690/1‑151 (MQ=255)
                                                                                   gCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATgg                                                                <  2:2267609/151‑1 (MQ=255)
                                                                                   gCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATgg                                                                >  2:9409422/1‑151 (MQ=255)
                                                                                    ctctCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGc                                                               <  2:597508/151‑1 (MQ=255)
                                                                                     tctcCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGcc                                                              <  2:2991086/151‑1 (MQ=255)
                                                                                           gcgATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATc                                                        <  1:3735615/151‑1 (MQ=255)
                                                                                            cgATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCa                                                       >  1:11601039/1‑151 (MQ=255)
                                                                                            cgATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCa                                                       <  1:1335232/151‑1 (MQ=255)
                                                                                             gaggCCCTGCGCTCCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTATTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTTATGGCCTAAATCaa                                                      <  2:7556684/148‑1 (MQ=255)
                                                                                              aTGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCAAt                                                     >  1:8631986/1‑151 (MQ=255)
                                                                                                 cccTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCAATggg                                                  <  2:11334971/151‑1 (MQ=255)
                                                                                                    tGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCAATGGGGCa                                               >  1:7358428/1‑151 (MQ=255)
                                                                                                    tGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCAATGGGGCa                                               >  2:5787580/1‑151 (MQ=255)
                                                                                                    tGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCAATGGGGCa                                               <  2:9081205/151‑1 (MQ=255)
                                                                                                       gcACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCAATGGGGCAttt                                            <  2:4188786/151‑1 (MQ=255)
                                                                                                        cACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTGCTTCGATTCGTTGTTTTAACTATATATATATTCTTCGATAGATGTAGATAATTTCACTGATGGCCTAAATCAATGGGGCAtttt                                           >  2:4800945/1‑151 (MQ=255)
                                                                                                           ccGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCAATGGGGCATTTTTGc                                        <  1:9352085/151‑1 (MQ=255)
                                                                                                              gCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCAATGGGGCATTTTTGCCGa                                     >  1:2155808/1‑151 (MQ=255)
                                                                                                              gCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCAATGGGGCATTTTTGCCGa                                     >  2:10090693/1‑151 (MQ=255)
                                                                                                                tGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCAATGGGGCATTTTTGCCGAAc                                   >  2:5333406/1‑151 (MQ=255)
                                                                                                                    tGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCAATGGGGCATTTTTGCCGAACCCCg                               <  1:903600/151‑1 (MQ=255)
                                                                                                                    tGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCAATGGGGCATTTTTGCCGAACCCCg                               <  2:2894046/151‑1 (MQ=255)
                                                                                                                     gCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCAATGGGGCATTTTTGCCGAACCCCGt                              <  2:6711105/151‑1 (MQ=255)
                                                                                                                      ccccGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTGTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCAATGGGGCATTTTTGCCGAACCCCGtt                             >  2:7214525/1‑151 (MQ=255)
                                                                                                                          ggTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCAATGGGGCATTTTTGCCGAACCCCGTTAAGa                         >  1:3450251/1‑151 (MQ=255)
                                                                                                                                  tGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCAATGGGGCATTTTTGCCGAACCCCGTTAAGATTCTCCCa                 <  2:2797395/151‑1 (MQ=255)
                                                                                                                                  tGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCAATGGGGCATTTTTGCCGAACCCCGTTAAGATTCTCCCa                 <  1:1987033/151‑1 (MQ=255)
                                                                                                                                   gggCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCAATGGGGCATTTTTGCCGAACCCCGTTAAGATTCTCCCAg                <  2:4625820/151‑1 (MQ=255)
                                                                                                                                    ggCCATCCGTGGAAGGCC‑GGTCACTGACGAGGGCAGGTTCTTCGATGAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACAGATGGCCTAAATCAATGGGGCATTTTTGCCGAACCCCGTTAAGATTCTCCCAGt               <  2:2080723/151‑1 (MQ=255)
                                                                                                                                    ggCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCAATGGGGCATTTTTGCCGAACCCCGTTAAGATTCTCCCAGt               <  2:11439911/151‑1 (MQ=255)
                                                                                                                                    ggCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCAATGGGGCATTTTTGCCGAACCCCGTTAAGATTCTCCCAGt               <  1:10880148/151‑1 (MQ=255)
                                                                                                                                    ggCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCAATGGGGCATTTTTGCCGAACCCCGTTAAGATTCTCCCAGt               <  1:7453416/151‑1 (MQ=255)
                                                                                                                                      ccAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCAATGGGGCATTTTTGCCGAACCCCGTTAAGATTCTCCCAGTCg             <  2:11022271/151‑1 (MQ=255)
                                                                                                                                                 aGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCAATGGGGCATTTTTGCCGAACCCCGTTAAGATTCTCCCAGTCGATTTGAAacac  >  2:6306894/1‑151 (MQ=255)
                                                                                                                                                      |                                                                                                                                                  
GTGGCCCAGGGGATCGGGCTGTCGATTCCGGCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCCGGGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCAATGGGGCATTTTTGCCGAACCCCGTTAAGATTCTCCCAGTCGATTTGAAACAC  >  NC_008463/155840‑156136

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑