Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 3,543,662 A→G T494A (ACG→GCG)  PA14_RS16110 → PucR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084633,543,6620AG100.0% 2269.6 / NA 575T494A (ACG→GCG) PA14_RS16110PucR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (240/335);  total (240/335)

Only 100 of 575 total aligned reads displayed.

GAACGCCGCGCAGCGGCTACACATACACAAGCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAACGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGACGCCGCCGCCAACAGGCGCGGCCAGGAACGGTTGCCTGGCCGCGG  >  NC_008463/3543514‑3543809
                                                                                                                                                    |                                                                                                                                                   
   cgccgcGCAGCGGCTACACATACACAAGCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAgg                                                                                                                                                <  2:4568913/151‑1 (MQ=255)
    gccgcGCAGCGGCTACACATACACAAGCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAggg                                                                                                                                               <  1:4967301/151‑1 (MQ=255)
    gccgcGCAGCGGCTACACATACACAAGCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGATGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAggg                                                                                                                                               >  2:397424/1‑151 (MQ=255)
      cgcgCAGCGGCTACACATACACAAGCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGc                                                                                                                                             >  1:3855893/1‑151 (MQ=255)
       gcgcAGCGGCTACACATACACAAGCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCa                                                                                                                                            <  1:4694184/151‑1 (MQ=255)
       gcgcAGCGGCTACACATACACAAGCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCa                                                                                                                                            <  2:8811980/151‑1 (MQ=255)
       gcgcAGCGGCTACACATACACAAGCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCa                                                                                                                                            <  1:2140682/151‑1 (MQ=255)
         gcAGCGGCTACACATACTCAAGCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAgg                                                                                                                                          <  2:5369102/151‑1 (MQ=255)
         gcAGCGGCTACACATACACAAGCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAgg                                                                                                                                          >  1:6941576/1‑151 (MQ=255)
            gCGGCTACACAGACACAAGCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACg                                                                                                                                       <  2:6699957/151‑1 (MQ=255)
             cGGCTACACATACACAAGCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGACATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCGCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGCAGCGAGGGGCAGGACgg                                                                                                                                      >  2:8301935/1‑151 (MQ=255)
             cGGCTACACATACACAAGCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACgg                                                                                                                                      >  2:9270978/1‑151 (MQ=255)
             cGGCTACACATACACAAGCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACgg                                                                                                                                      <  1:7448722/151‑1 (MQ=255)
               gCTACACATACACAAGCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCg                                                                                                                                    <  2:5945653/151‑1 (MQ=255)
               gCTACACATACACAAGCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCg                                                                                                                                    >  2:10560288/1‑151 (MQ=255)
               gCTACACATACACAAGCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCg                                                                                                                                    <  1:611595/151‑1 (MQ=255)
                cTACACATACACAAGCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGa                                                                                                                                   <  2:11157976/151‑1 (MQ=255)
                 tacacctacacaAGCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGaa                                                                                                                                  <  1:1142684/151‑1 (MQ=255)
                  acacatacacaAGCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAag                                                                                                                                 >  1:3284108/1‑151 (MQ=255)
                      atacacaAGCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGagca                                                                                                                             <  2:2224101/151‑1 (MQ=255)
                        acactAGCAGAGCCTGGTCTCCCGGATACGCCGCATCGAGGAACTCAGCGGTCGCTCGCTGGACAGCACCGCCGTTGTGGCGACCCTCTGGGTCGCCTTGCAGGCTACCCGGCTGTCGCCGGATGCGAGGGGCCGGTCGGCGAAGagcagc                                                                                                                           >  2:6927799/1‑151 (MQ=255)
                        acacaAGCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGagcagc                                                                                                                           <  1:5438737/151‑1 (MQ=255)
                            aaGCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGcctcc                                                                                                                       <  2:35521/151‑1 (MQ=255)
                              gCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTg                                                                                                                     <  1:7945619/151‑1 (MQ=255)
                               cAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTgg                                                                                                                    <  2:112234/151‑1 (MQ=255)
                                agttccTGGTCTACCTGATACGCCGCATCGAGGAAATCAGCGTTCGATCGCTGGACAGCACCGCCGATGTGGCGACCATCTGGTTCGCCTTTCAGGCAACCGGGCTGTCGCCTGAAGCGAGGGGCTGTACGGGGAAGAGCTGCCTCCTGGa                                                                                                                   <  2:6183928/147‑1 (MQ=255)
                                agagCCTGGTCTACCGGATACGACGCATCGAGGTAATCAGCGGTCGCACGCTGGAGAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCTACCCGGCTGTCGCCGGAAGCGAGGGGCAGGTCGGCGAAGAGCAGCCTCCTGGa                                                                                                                   >  1:3984356/1‑151 (MQ=255)
                                 gagCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAg                                                                                                                  >  2:4454453/1‑151 (MQ=255)
                                  agCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGc                                                                                                                 >  2:3079096/1‑151 (MQ=255)
                                   gCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCa                                                                                                                >  2:7369449/1‑151 (MQ=255)
                                      tGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTc                                                                                                             >  2:8053707/1‑151 (MQ=255)
                                       ggTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTcc                                                                                                            >  1:7226431/1‑151 (MQ=255)
                                       ggTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTcc                                                                                                            >  2:4767272/1‑151 (MQ=255)
                                               ggATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCa                                                                                                    <  2:7835038/151‑1 (MQ=255)
                                               ggATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCa                                                                                                    >  1:10636463/1‑151 (MQ=255)
                                               ggATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCa                                                                                                    >  2:6811169/1‑151 (MQ=255)
                                                gATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCAt                                                                                                   <  1:1238140/151‑1 (MQ=255)
                                                gATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCAt                                                                                                   >  1:2929539/1‑151 (MQ=255)
                                                 aTACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATg                                                                                                  <  1:8728292/151‑1 (MQ=255)
                                                       cgcATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGgcggcg                                                                                            >  1:1975192/1‑151 (MQ=255)
                                                        gcATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGa                                                                                           <  1:1103720/151‑1 (MQ=255)
                                                        gcATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGa                                                                                           >  2:3322242/1‑151 (MQ=255)
                                                         cATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGAc                                                                                          >  1:778231/1‑151 (MQ=255)
                                                            cGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATg                                                                                       >  2:103335/1‑151 (MQ=255)
                                                            cGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATg                                                                                       <  1:2697507/151‑1 (MQ=255)
                                                                gAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACt                                                                                   <  2:124773/151‑1 (MQ=255)
                                                                gAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACt                                                                                   >  1:9101572/1‑151 (MQ=255)
                                                                 aaaTCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTg                                                                                  <  1:4840588/151‑1 (MQ=255)
                                                                  aaTCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGa                                                                                 >  1:665729/1‑151 (MQ=255)
                                                                    tCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGAcg                                                                               <  1:2370318/151‑1 (MQ=255)
                                                                         ggTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGa                                                                          >  2:9688306/1‑151 (MQ=255)
                                                                          gTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAg                                                                         >  1:3529015/1‑151 (MQ=255)
                                                                          gTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAg                                                                         <  1:8497312/151‑1 (MQ=255)
                                                                          gTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAg                                                                         >  1:1635735/1‑151 (MQ=255)
                                                                            cgctcgctGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGcc                                                                       <  2:5267668/151‑1 (MQ=255)
                                                                             gcgcgctGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCGGGCAGGCAACCCGGCTGTCGGCGGAAGCGAGGGGCAGGACGGCGAAGCGCAGCCTCCTGGAGCAGTCCGCGCACCCTGGCGGCGACAGGGACTGACGGCGGAGccc                                                                      >  1:7547865/4‑151 (MQ=255)
                                                                              ctcgctGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCg                                                                     <  1:7043445/151‑1 (MQ=255)
                                                                                cgctGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGAt                                                                   >  1:2501216/1‑151 (MQ=255)
                                                                                 gctGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATa                                                                  <  2:8588238/151‑1 (MQ=255)
                                                                                  ctGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATAc                                                                 <  1:8329763/151‑1 (MQ=255)
                                                                                   tGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATAcc                                                                <  2:5013864/151‑1 (MQ=255)
                                                                                   tGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATAcc                                                                <  2:11515029/151‑1 (MQ=255)
                                                                                      aCAGCACCGCCGATGTGGCGAGCCTCTGGGTCGCCTTGCAGGCATCCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGGGCCCGATACCTgg                                                             >  2:4218752/1‑151 (MQ=255)
                                                                                        aGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGgcg                                                           <  1:9334526/151‑1 (MQ=255)
                                                                                        aGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGgcg                                                           <  2:2400427/151‑1 (MQ=255)
                                                                                          cACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCt                                                         <  1:3425480/151‑1 (MQ=255)
                                                                                               ccgATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAg                                                    <  2:2813173/151‑1 (MQ=255)
                                                                                                 gATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGga                                                  >  2:5855879/1‑151 (MQ=255)
                                                                                                  aTGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTTGCGCTTGCAGgac                                                 <  1:981103/151‑1 (MQ=255)
                                                                                                  aTGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGgac                                                 <  1:1342947/151‑1 (MQ=255)
                                                                                                     tgGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGgacgac                                              <  1:8240571/151‑1 (MQ=255)
                                                                                                      ggCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGacgacg                                             <  1:1445114/151‑1 (MQ=255)
                                                                                                       gCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGAcgc                                            <  1:9445114/151‑1 (MQ=255)
                                                                                                         gACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGAcgccg                                          >  1:5194864/1‑151 (MQ=255)
                                                                                                         gACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGAcgccg                                          >  2:10876743/1‑151 (MQ=255)
                                                                                                           cccTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGAcgccgcc                                        <  2:1984518/151‑1 (MQ=255)
                                                                                                           cccTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGAcgccgcc                                        <  2:10004305/151‑1 (MQ=255)
                                                                                                            ccTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGAcgccgccg                                       >  2:6568846/1‑151 (MQ=255)
                                                                                                            ccTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGAcgccgccg                                       <  2:7077141/151‑1 (MQ=255)
                                                                                                                 ggTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGACGCCGCCGCCAAc                                  <  1:6308663/151‑1 (MQ=255)
                                                                                                                 ggTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGACGCCGCCGCCAAc                                  >  2:2104619/1‑151 (MQ=255)
                                                                                                                 ggTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGACGCCGCCGCCAAc                                  >  1:9644836/1‑151 (MQ=255)
                                                                                                                  gTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGACGCCGCCGCCAACa                                 >  2:985196/1‑151 (MQ=255)
                                                                                                                  gTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGACGCCGCCGCCAACa                                 <  2:6410477/151‑1 (MQ=255)
                                                                                                                  gTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGACGCCGCCGCCAACa                                 <  2:1808246/151‑1 (MQ=255)
                                                                                                                    tCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGACGCCGCCGCCAACAgg                               >  2:3269716/1‑151 (MQ=255)
                                                                                                                      gCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGACGCCGCCGCCAACAGgcg                             <  1:10001081/151‑1 (MQ=255)
                                                                                                                       ccTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCCGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGACGCCGCCGCCAACAGgcgc                            >  1:5732780/1‑151 (MQ=255)
                                                                                                                          tGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGACGCCGCCGCCAACAGGCGCGGc                         <  2:1551001/151‑1 (MQ=255)
                                                                                                                          tGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGACGCCGCCGCCAACAGGCGCGGc                         <  1:10491753/151‑1 (MQ=255)
                                                                                                                           gCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGACGCCGCCGCCAACAGGCGCGGcc                        <  2:9469941/151‑1 (MQ=255)
                                                                                                                                 aaCCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGACGCCGCCGCCAACAGGCGCGGCCAGGAAc                  <  1:6428978/151‑1 (MQ=255)
                                                                                                                                  aCCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGACGCCGCCGCCAACAGGCGCGGCCAGGAACg                 <  2:10179410/151‑1 (MQ=255)
                                                                                                                                   cccGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGACGCCGCCGCCAACAGGCGCGGCCAGGAACgg                >  1:4612863/1‑151 (MQ=255)
                                                                                                                                      ggCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGACGCCGCCGCCAACAGGCGCGGCCAGGAACGGTTg             <  2:9184530/151‑1 (MQ=255)
                                                                                                                                      ggCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGACGCCGCCGCCAACAGGCGCGGCCAGGAACGGTTg             <  1:11086750/151‑1 (MQ=255)
                                                                                                                                          gTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGACGCCGCCGCCAACAGGCGCGGCCAGGAACGGTTGCCTg         <  2:5707788/151‑1 (MQ=255)
                                                                                                                                          gTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGACGCCGCCGCCAACAGGCGCGGCCAGGAACGGTTGCCTg         <  1:4220724/151‑1 (MQ=255)
                                                                                                                                              ccGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGACGCCGCCGCCAACAGGCGCGGCCAGGAACGGTTGCCTGGCcg     >  1:8961803/1‑151 (MQ=255)
                                                                                                                                               cGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGAACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGACGCCGCCGCCAACAGGCGCGGCCAGGAACGGTTGCCTGGCcgc    >  1:5783222/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GAACGCCGCGCAGCGGCTACACATACACAAGCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAACGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGACGCCGCCGCCAACAGGCGCGGCCAGGAACGGTTGCCTGGCCGCGG  >  NC_008463/3543514‑3543809

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑