Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 4,565,040 A→G F307F (TTT→TTC)  PA14_RS20885 ← anthranilate synthase component 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084634,565,0400AG99.9% 2655.2 / ‑4.7 673F307F (TTT→TTC) PA14_RS20885anthranilate synthase component 1
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (324/348);  minor base C (0/1);  total (324/349)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 675 total aligned reads displayed.

GGCGCAACTCCGCTTCCAGGCGATTGTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCCCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCAAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCCCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGCCGGCCCTCACG  >  NC_008463/4564892‑4565187
                                                                                                                                                    |                                                                                                                                                   
 gcgcAACTCCGCTTCCAGGCGATTGTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAAc                                                                                                                                                  <  2:2023080/151‑1 (MQ=255)
    cAACTCCGCTTCCAGGCGATTGTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAgg                                                                                                                                               <  1:74495/151‑1 (MQ=255)
       cTCCGCTTCCAGGCGATTGTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAg                                                                                                                                            >  2:943205/1‑151 (MQ=255)
        tCCGCTTCCAGGCGATTGTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGa                                                                                                                                           <  1:9551911/151‑1 (MQ=255)
         ccGCTTCCAGGCGATTGTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGaa                                                                                                                                          >  1:2219361/1‑151 (MQ=255)
               ccAGGCGATTGTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTcccc                                                                                                                                    <  2:7065951/151‑1 (MQ=255)
               ccAGGCGATTGTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTcccc                                                                                                                                    <  1:9126669/151‑1 (MQ=255)
                cAGGCGATTGTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTccccc                                                                                                                                   >  1:5645433/1‑151 (MQ=255)
                 aGGCGATTGTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCg                                                                                                                                  >  2:6307156/1‑151 (MQ=255)
                   gCGATTGTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCa                                                                                                                                >  1:10047314/1‑151 (MQ=255)
                   gCGATTGTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCa                                                                                                                                >  1:4232881/1‑151 (MQ=255)
                   gCGATTGTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCa                                                                                                                                <  2:8120847/151‑1 (MQ=255)
                     gATTGTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATc                                                                                                                              >  2:735323/1‑151 (MQ=255)
                     gATTGTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATc                                                                                                                              <  1:8299478/151‑1 (MQ=255)
                     gATTGTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATc                                                                                                                              >  2:10625080/1‑151 (MQ=255)
                     gATTGTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATc                                                                                                                              >  1:3514311/1‑151 (MQ=255)
                     gATTGTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATc                                                                                                                              <  1:4936196/151‑1 (MQ=255)
                     gATTGTCCAGTTCCGCATCGAGGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCACGGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATc                                                                                                                              <  1:6498333/151‑1 (MQ=255)
                       ttGTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGa                                                                                                                            >  2:10898922/1‑151 (MQ=255)
                       ttGTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGa                                                                                                                            >  2:1845060/1‑151 (MQ=255)
                        tGTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAg                                                                                                                           <  2:11352648/151‑1 (MQ=255)
                        tGTCCAGGTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCATAGGGGTAGAGGTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAg                                                                                                                           <  2:6025003/151‑1 (MQ=255)
                          tCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGga                                                                                                                         <  1:1642680/151‑1 (MQ=255)
                          tCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGga                                                                                                                         >  1:2695889/1‑151 (MQ=255)
                          tCCAGGTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGga                                                                                                                         <  2:5139102/151‑1 (MQ=255)
                            cAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCACTGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGgaag                                                                                                                       <  2:5246618/151‑1 (MQ=255)
                                    cATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTa                                                                                                               >  2:2991790/1‑151 (MQ=255)
                                      tCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACg                                                                                                             <  1:9321660/151‑1 (MQ=255)
                                        gATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACggg                                                                                                           >  1:8430391/1‑151 (MQ=255)
                                        gATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACggg                                                                                                           <  2:1731039/151‑1 (MQ=255)
                                           ggCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTg                                                                                                        <  2:4097125/151‑1 (MQ=255)
                                            gcgcCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTgg                                                                                                       <  2:10165947/151‑1 (MQ=255)
                                                cccGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGtt                                                                                                   <  1:11154897/151‑1 (MQ=255)
                                                cccGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGtt                                                                                                   <  2:7984182/151‑1 (MQ=255)
                                                  cGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGc                                                                                                 <  1:6228519/151‑1 (MQ=255)
                                                  cGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGc                                                                                                 <  2:4933733/151‑1 (MQ=255)
                                                     gCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCa                                                                                              <  2:1384316/151‑1 (MQ=255)
                                                       aTCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAgg                                                                                            >  2:8955303/1‑151 (MQ=255)
                                                         cgcgTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGAGTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGca                                                                                          >  1:2594573/1‑151 (MQ=255)
                                                           cgTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGcaca                                                                                        <  1:5120276/151‑1 (MQ=255)
                                                              ccGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACt                                                                                     <  2:6590719/151‑1 (MQ=255)
                                                                gcgcgGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTgg                                                                                   <  1:11003835/151‑1 (MQ=255)
                                                                 cgcgGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGc                                                                                  <  1:4430272/151‑1 (MQ=255)
                                                                  gcgGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCg                                                                                 >  1:11450184/1‑151 (MQ=255)
                                                                   cgGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGa                                                                                >  1:9775797/1‑151 (MQ=255)
                                                                    gggCGAGTGCCGTCTATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGAt                                                                               <  1:2085367/151‑1 (MQ=255)
                                                                     ggCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCCCAACTGGCGATa                                                                              >  2:5879682/1‑151 (MQ=255)
                                                                     ggCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATa                                                                              >  2:5328655/1‑151 (MQ=255)
                                                                      gCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAg                                                                             <  1:5501264/151‑1 (MQ=255)
                                                                      gCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAg                                                                             <  1:11350404/151‑1 (MQ=255)
                                                                        gAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGc                                                                           <  2:3495923/151‑1 (MQ=255)
                                                                            gCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGc                                                                       >  2:4287535/1‑151 (MQ=255)
                                                                               ggCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAg                                                                    >  1:8894918/1‑151 (MQ=255)
                                                                               ggCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAg                                                                    <  1:5274328/151‑1 (MQ=255)
                                                                                 cAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAggg                                                                  <  1:879661/151‑1 (MQ=255)
                                                                                  aaTGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAgggg                                                                 <  1:1182004/151‑1 (MQ=255)
                                                                                  aaTGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAgggg                                                                 >  2:3134216/1‑151 (MQ=255)
                                                                                     ggggTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTACAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTcc                                                              <  2:10123588/151‑1 (MQ=255)
                                                                                      gggTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCcg                                                             <  2:8828671/151‑1 (MQ=255)
                                                                                       ggTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCcgc                                                            <  2:1632940/151‑1 (MQ=255)
                                                                                       ggTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCcgc                                                            <  1:7050973/151‑1 (MQ=255)
                                                                                              ttCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGc                                                     >  1:3930503/1‑151 (MQ=255)
                                                                                               tCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCa                                                    <  2:10490613/151‑1 (MQ=255)
                                                                                                 cACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATg                                                  <  2:973414/151‑1 (MQ=255)
                                                                                                 cACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATg                                                  <  1:7327770/151‑1 (MQ=255)
                                                                                                 cACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATg                                                  >  1:10546139/1‑151 (MQ=255)
                                                                                                  aCCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCCCAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGc                                                 >  2:4559226/1‑151 (MQ=255)
                                                                                                  aCCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGc                                                 >  2:3686881/1‑151 (MQ=255)
                                                                                                       gcgACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAg                                            <  1:10372852/151‑1 (MQ=255)
                                                                                                       gcgACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAg                                            <  2:5072567/151‑1 (MQ=255)
                                                                                                        cgACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGc                                           >  1:9955588/1‑151 (MQ=255)
                                                                                                         gACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCt                                          <  1:5819632/151‑1 (MQ=255)
                                                                                                          aCTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTg                                         >  1:10817729/1‑151 (MQ=255)
                                                                                                           ctctCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTgc                                        >  1:865897/1‑151 (MQ=255)
                                                                                                           ctctCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTgc                                        >  2:5517254/1‑151 (MQ=255)
                                                                                                           ctctCCCCGCCGTACTTCCCAGCCGCCTCGGGCGCAGCGCCGACCAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTgc                                        <  2:11216842/151‑1 (MQ=255)
                                                                                                                cgcgtcgtACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGAc                                   <  2:6904799/149‑1 (MQ=255)
                                                                                                                 gcgtcgtACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACg                                  <  2:828740/150‑1 (MQ=255)
                                                                                                                  cgtcgtACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACgg                                 >  1:1770197/1‑151 (MQ=255)
                                                                                                                   gtcgtACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGt                                <  1:6987163/151‑1 (MQ=255)
                                                                                                                   gtcgtACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGt                                <  1:7786350/151‑1 (MQ=255)
                                                                                                                      gtACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCCGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACg                             >  2:2655800/1‑151 (MQ=255)
                                                                                                                      gtACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACg                             >  1:1331823/1‑151 (MQ=255)
                                                                                                                      gtACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACg                             >  2:8479335/1‑151 (MQ=255)
                                                                                                                       tACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGa                            >  1:3122077/1‑151 (MQ=255)
                                                                                                                        aCTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGAt                           <  2:11468902/151‑1 (MQ=255)
                                                                                                                        aCTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGAt                           <  2:7828752/151‑1 (MQ=255)
                                                                                                                          ttCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTCCGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCt                         >  2:4877449/1‑151 (MQ=255)
                                                                                                                           tCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGACGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTg                        <  2:4769975/151‑1 (MQ=255)
                                                                                                                            cAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTgg                       >  2:2446323/1‑151 (MQ=255)
                                                                                                                             aaTGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGa                      >  1:1272550/1‑151 (MQ=255)
                                                                                                                              aTGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGaa                     >  2:5687849/1‑151 (MQ=255)
                                                                                                                               tGCCGACTCCGGCGAAGCGCCGACCAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAac                    <  2:2839415/151‑1 (MQ=255)
                                                                                                                               tGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAac                    >  1:1026543/1‑151 (MQ=255)
                                                                                                                                gCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAaca                   >  2:3791550/1‑151 (MQ=255)
                                                                                                                                   gACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGt                >  2:7618657/1‑151 (MQ=255)
                                                                                                                                       ccGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGcc            <  2:3327778/151‑1 (MQ=255)
                                                                                                                                            gAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGCCGGccc       >  1:7196133/1‑151 (MQ=255)
                                                                                                                                              aGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTCCGATCTGGAACACGTCGCCGGCCCTc     >  2:9171134/1‑151 (MQ=255)
                                                                                                                                              aGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGCCGGCCCTc     >  1:7900629/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GGCGCAACTCCGCTTCCAGGCGATTGTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCCCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCAAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCCCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGCCGGCCCTCACG  >  NC_008463/4564892‑4565187

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑