Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 5,530,315 A→G K229K (AAA→AAG)  PA14_RS25330 → iron ABC transporter substrate‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084635,530,3150AG100.0% 1422.1 / NA 362K229K (AAA→AAG) PA14_RS25330iron ABC transporter substrate‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (205/157);  total (205/157)

Only 100 of 390 total aligned reads displayed.

TCAAGGGCCAGGAGGAAGCCGAGGATTGGCTGACCGGCCTGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAAAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCCAGCAGTTCGTCGCCTTCATGCTCAGCGAG  >  NC_008463/5530167‑5530462
                                                                                                                                                    |                                                                                                                                                   
  aaGGGCCAGGAGGAAGCCGAGGATTGGCTGACCGGCCTGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAagaagg                                                                                                                                                 >  2:2544198/1‑151 (MQ=255)
     ggCCAGGAGGAAGCCGAGGATTGGCTGACCGGCCTGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAagaaggaaa                                                                                                                                              <  1:8106339/151‑1 (MQ=255)
     ggCCAGGAGGAAGCCGAGGATTGGCTGACCGGCCTGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAagaaggaaa                                                                                                                                              <  1:1454345/151‑1 (MQ=255)
          ggaggaAGCCGAGGATTGGCTGACCGGCCTGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGc                                                                                                                                         >  2:2413964/1‑151 (MQ=255)
           gaggcACCCGAGGATTGGCTGACCGTCCAGACGGCCTTCCGCTAGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCg                                                                                                                                        <  2:9758938/151‑1 (MQ=255)
           gaggaAGCCGAGGATTGGCTGACCGGCCTGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCg                                                                                                                                        <  1:8182442/151‑1 (MQ=255)
           gaggaAGCCGAGGATTGGCTGACCGGCCTGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCg                                                                                                                                        <  1:1401345/151‑1 (MQ=255)
             ggaAGCCGAGGATTGGCTGACCGGCCTGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAg                                                                                                                                      <  1:2573796/151‑1 (MQ=255)
              gaAGCCGAGGATTGGCTGACCGGCCTGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGt                                                                                                                                     >  2:4587835/1‑151 (MQ=255)
               aaGCCGAGGATTGGCTGACCGGCCTGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGtt                                                                                                                                    <  2:10784363/151‑1 (MQ=255)
               aaGCCGAGGATTGGCTGACCGGCCTGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGtt                                                                                                                                    >  2:2187729/1‑151 (MQ=255)
                aGCCGAGGATTGGCTGCCCGGCCTGAAGGCCTTCGGCTCGATCTACACCAACACCGACACCGCCATGAAAGCCGTGGAGAACGGCGCGGTCGACATGGCGCTGAGCAACAACTACTACTGGGACACCCTGAAGAAGGAAAAGGGCGAGTTg                                                                                                                                   <  1:1112401/151‑1 (MQ=255)
                aGCCGAGGATTGGCTGACCGGCCTGAAGGCCTTCGGCTCGATCTCCACCAACAACGTCACCGCAATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGtag                                                                                                                                   >  2:7908068/1‑149 (MQ=255)
                   cGAGGATTGGCTGACCGGCCTGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAAc                                                                                                                                >  2:6603418/1‑151 (MQ=255)
                     aGGATTGGCTGACCGGCCTGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTc                                                                                                                              <  1:5038151/151‑1 (MQ=255)
                      ggATTGGCTGACCGGCCTGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTcg                                                                                                                             >  1:10671714/1‑151 (MQ=255)
                          tGGCTGACCGGCCTGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGcc                                                                                                                         >  1:6109734/1‑151 (MQ=255)
                          tGGCTGACCGGCCTGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGcc                                                                                                                         >  2:7694982/1‑151 (MQ=255)
                           ggCTGACCGGCCTGAAGTCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACGACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCt                                                                                                                        <  1:2998252/151‑1 (MQ=255)
                              tGACCGGCCTGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCa                                                                                                                     >  1:2218077/1‑151 (MQ=255)
                                  cGGCCTGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCactac                                                                                                                 >  1:9433768/1‑151 (MQ=255)
                                   ggCCTGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCCTGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCactact                                                                                                                >  2:7116694/1‑151 (MQ=255)
                                   ggCCTGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCactact                                                                                                                >  1:467211/1‑151 (MQ=255)
                                     ccTGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTc                                                                                                              >  1:3217956/1‑151 (MQ=255)
                                       tGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCgg                                                                                                            >  1:274550/1‑151 (MQ=255)
                                        gAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCATCAACTACGCCTGGTACACCGTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACGTCTGCGGc                                                                                                           >  2:5939614/1‑151 (MQ=255)
                                        gAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGc                                                                                                           >  1:10987280/1‑151 (MQ=255)
                                            gCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAAcc                                                                                                       <  2:5246345/151‑1 (MQ=255)
                                            gCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAAcc                                                                                                       >  1:6954154/1‑151 (MQ=255)
                                             ccTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCa                                                                                                      <  1:5850124/151‑1 (MQ=255)
                                              cTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAg                                                                                                     >  1:1952992/1‑151 (MQ=255)
                                                tctgCTAGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGa                                                                                                   <  2:10153279/148‑1 (MQ=255)
                                                tCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGa                                                                                                   <  2:9110202/151‑1 (MQ=255)
                                                tCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGa                                                                                                   <  2:3398008/151‑1 (MQ=255)
                                                  ggCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGAcc                                                                                                 >  1:8967535/1‑151 (MQ=255)
                                                  ggCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGAcc                                                                                                 >  2:7457887/1‑151 (MQ=255)
                                                     tCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCgg                                                                                              <  1:7221092/151‑1 (MQ=255)
                                                        aTCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGgcg                                                                                           >  2:1816819/1‑151 (MQ=255)
                                                         tCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGgcgc                                                                                          <  2:11400826/151‑1 (MQ=255)
                                                          cTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGgcgcg                                                                                         >  1:9953708/1‑151 (MQ=255)
                                                            acacCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCt                                                                                       >  1:5258599/1‑151 (MQ=255)
                                                             cacCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTg                                                                                      >  1:11247869/1‑151 (MQ=255)
                                                              acCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTgg                                                                                     >  2:2873129/1‑151 (MQ=255)
                                                              acCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTgg                                                                                     >  1:2384563/1‑151 (MQ=255)
                                                               ccAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGt                                                                                    >  2:3497958/1‑151 (MQ=255)
                                                                 aacaacGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCa                                                                                  <  2:10557893/151‑1 (MQ=255)
                                                                   caacGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCAcc                                                                                >  1:9627818/1‑151 (MQ=255)
                                                                       gTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGtct                                                                            >  1:2196509/1‑151 (MQ=255)
                                                                       gTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGtct                                                                            >  2:2710518/1‑151 (MQ=255)
                                                                        tCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGtctc                                                                           >  1:4346561/1‑151 (MQ=255)
                                                                         cACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTcc                                                                          <  1:10857203/151‑1 (MQ=255)
                                                                           ccGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCgg                                                                        <  2:8727425/151‑1 (MQ=255)
                                                                           ccGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCgg                                                                        <  1:2568908/151‑1 (MQ=255)
                                                                            cGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGgc                                                                       >  2:8534130/1‑151 (MQ=255)
                                                                             gCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGgcg                                                                      >  2:9435935/1‑151 (MQ=255)
                                                                              ccATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGgcgc                                                                     >  1:11419443/1‑151 (MQ=255)
                                                                              ccATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGgcgc                                                                     >  1:3512590/1‑151 (MQ=255)
                                                                               cATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGcgcc                                                                    <  2:4785963/151‑1 (MQ=255)
                                                                                 tGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGcgccgc                                                                  >  1:6455979/1‑151 (MQ=255)
                                                                                   aaaGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAg                                                                >  2:4080774/1‑151 (MQ=255)
                                                                                    aaGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGt                                                               <  1:5989358/151‑1 (MQ=255)
                                                                                      gCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCACCCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGc                                                             >  2:11203536/1‑151 (MQ=255)
                                                                                        cGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTc                                                           >  1:8237121/1‑151 (MQ=255)
                                                                                           ggAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAg                                                        >  1:10545710/1‑151 (MQ=255)
                                                                                           ggAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAg                                                        <  1:10171169/151‑1 (MQ=255)
                                                                                             agaACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTc                                                      >  1:2666312/1‑151 (MQ=255)
                                                                                              gaACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTcc                                                     <  2:8463833/151‑1 (MQ=255)
                                                                                              gaACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTcc                                                     <  1:7288336/151‑1 (MQ=255)
                                                                                               aaCGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCa                                                    <  1:5562252/151‑1 (MQ=255)
                                                                                               aaCGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCa                                                    >  1:7580867/1‑151 (MQ=255)
                                                                                                aCGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGACGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCag                                                   >  1:7530844/1‑151 (MQ=255)
                                                                                                    cGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACACAGAAGAAGCAAAAGTGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGAGCACAAGTCCagcaag                                               >  1:10373688/1‑151 (MQ=255)
                                                                                                      aGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCagcaagca                                             <  1:10218316/151‑1 (MQ=255)
                                                                                                          cGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCAcccc                                         >  2:1177829/1‑151 (MQ=255)
                                                                                                          cGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCAcccc                                         >  1:9803012/1‑151 (MQ=255)
                                                                                                             cATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAgg                                      >  1:11377231/1‑151 (MQ=255)
                                                                                                             cATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAgg                                      >  2:5169933/1‑151 (MQ=255)
                                                                                                              aTGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAggg                                     <  1:10062065/151‑1 (MQ=255)
                                                                                                                 gcgcTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAg                                  >  1:6829460/1‑151 (MQ=255)
                                                                                                                  cgcTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGc                                 >  2:1578717/1‑151 (MQ=255)
                                                                                                                   gcTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGcc                                <  1:1761398/151‑1 (MQ=255)
                                                                                                                   gcTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGcc                                >  1:4068242/1‑151 (MQ=255)
                                                                                                                     tGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGTGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCca                              <  1:8499047/151‑1 (MQ=255)
                                                                                                                     tGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCca                              <  2:5794176/151‑1 (MQ=255)
                                                                                                                     tGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCca                              >  2:7395831/1‑151 (MQ=255)
                                                                                                                      gATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCcag                             <  2:6362059/151‑1 (MQ=255)
                                                                                                                      gATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCcag                             <  2:10396700/151‑1 (MQ=255)
                                                                                                                       aTCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCcagc                            <  2:11092495/151‑1 (MQ=255)
                                                                                                                            caacTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCCAGCAGTtc                       <  1:3851242/151‑1 (MQ=255)
                                                                                                                             aacTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCCAGCAGTtcg                      >  1:9557864/1‑151 (MQ=255)
                                                                                                                              actactactGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCCAGCAGTtcgt                     <  2:10285286/151‑1 (MQ=255)
                                                                                                                               ctactactGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCCAGCAGTtcgtc                    <  1:6614275/151‑1 (MQ=255)
                                                                                                                                  ctactGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCCAGCAGTTCGTCGcc                 >  1:934263/1‑151 (MQ=255)
                                                                                                                                        gTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCCAGCAGTTCGTCGCCTTCATg           >  1:2853672/1‑151 (MQ=255)
                                                                                                                                           cacCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCCAGCAGTTCGTCGCCTGCATGCTc        >  1:1181448/1‑151 (MQ=255)
                                                                                                                                            acCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCCAGCAGTTCGTCGCCTTCATGCTCa       <  1:7835570/151‑1 (MQ=255)
                                                                                                                                             cccTGAAGAAGGAACAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCCAGCAGTTCGTCGCCTTCATGCTCAg      <  2:6921959/151‑1 (MQ=255)
                                                                                                                                               cTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCCAGCAGTTCGTCGCCTTCATGCTCAGCg    <  2:3186293/151‑1 (MQ=255)
                                                                                                                                               cTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCCAGCAGTTCGTCGCCTTCATGCTCAGCg    >  1:7156285/1‑151 (MQ=255)
                                                                                                                                                tGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCCAGCAGTTCGTCGCCTTCATGCTCAGCGa   <  1:7106782/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TCAAGGGCCAGGAGGAAGCCGAGGATTGGCTGACCGGCCTGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAAAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCCAGCAGTTCGTCGCCTTCATGCTCAGCGAG  >  NC_008463/5530167‑5530462

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑