Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 5,757,527 G→C P26R (CCC→CGC)  PA14_RS26415 ← ferredoxin reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084635,757,5270GC99.8% 1815.9 / NA 467P26R (CCC→CGC) PA14_RS26415ferredoxin reductase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base C (217/249);  minor base A (0/1);  total (217/250)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 467 total aligned reads displayed.

CGCGCCGGCGGCCAGCCGGGGGCGGTACGCAGGGCCAGGGCGAGCATATCGTCGGCCACCCAGTAGCGTGCCTCGACCCGCGCATAGTGGCGGTTCAGCGACGCCAGCGGATGGGCGTGGCGCAGCAGGCCGTCGACATCGGCCTGGGGCAGCCAGCCACGGGCGGCGAGACGGCGCAGGGGTGCGAGCAAAGGGGTCAGGAGAGCGAGGGGAAGCAAGGCCATCGAGAATATTTCAGTGAACGATCGTTCACAAAACTTTGCCCGTTCCGCGCAATCGAGTCAACAAGCGT  >  NC_008463/5757380‑5757671
                                                                                                                                                   |                                                                                                                                                
  cgcCGGCGGCCAGCCGGGGGCGGTACGCAGGGCCAGGGCGAGCATATCGTCGGCCACCCAGTAGCGTGCCTCGACCCGCGCATAGTGGCGGTTCAGCGACGCCAGCGGATGGGCGTGGCGCAGCAGGCCGTCGACATCGGCCTCGCGcagc                                                                                                                                             >  1:6928796/1‑151 (MQ=255)
  cgcCGGCGGCCAGCCGGGGGCGGTACGCAGGGCCAGGGCGAGCATATCGTCGGCCACCCAGTAGCGTGCCTCGACCCGCGCATAGTGGCGGTTCAGCGACGCCAGCGGATGGGCGTGGCGCAGCAGGCCGTCGACATCGGCCTCGCGcagc                                                                                                                                             >  1:3097/1‑151 (MQ=255)
    ccGGCGGCCAGCCGGGGGCGGTACGCAGGGCCAGGGCGAGCATATCGTCGGCCACCCAGTAGCGTGCCTCGACCCGCGCATAGTGGCGGTTCAGCGACGCCAGCGGATGGGCGTGGCGCAGCAGGCCGTCGACATCGGCCTCGCGcagcca                                                                                                                                           <  2:8956400/151‑1 (MQ=255)
       gcggcCAGCCGGGGGCGGTACGCAGGGCCAGGGCGAGCATATCGTCGGCCACCCAGTAGCGTGCCTCGACCCGCGCATAGTGGCGGTTCAGCGACGCCAGCGGATGGGCGTGGCGCAGCAGGCCGTCGACATCGGCCTCGCGCagccagcc                                                                                                                                        <  2:7932901/151‑1 (MQ=255)
        cggcCAGCCGGGGGCGGTACGCAGGGCCAGGGCGAGCATATCGTCGGCCACCCAGTAGCGTGCCTCGACCCGCGCATAGTGGCGGTTCAGCGACGCCAGCGGATGGGCGTGGCGCAGCAGGCCGTCGACATCGGCCTCGCGCAgccagcca                                                                                                                                       <  2:9726969/151‑1 (MQ=255)
           ccAGCGGGGGGCGGTACGCCGGGCCCGGGCGAGCATATCGTCGGCCACCCAGTAGCGGGCCTCGACCCGCGCATTGTGGCGGTTCAGCGACGCCAGCGGATGGGCGTGGCGCAGCAGGCCGTCGACATCGGCCTCGCGCAGCCAGCCACgg                                                                                                                                    <  2:10622594/151‑1 (MQ=255)
              gCCGGGGGCGGGTCGCTGGGCCTGGGCGAGCATCTCGTCGGCCACCCCGTAGCGTGCCTCGACCCGCGCATAGTGGCGGTTCAGCGACGCCAGCGGATGGGCGTGGCGCAGCAGGCCGTCGACATCGGCCTCGCGCAGCCAGCCACGggcg                                                                                                                                 <  2:8126509/151‑1 (MQ=255)
                 gggggCGGTACGCAGGGCCAGGGCGAGCATATCGTCGGCCACCCAGTAGCGTGCCTCGACCCGCGCATAGTGGCGGTTCAGCGACGCCAGCGGATGGGCGTGGCGCAGCAGGCCGTCGACATCGGCCTCGCGCAGCCAGCCACGGgcggcg                                                                                                                              >  2:10163593/1‑151 (MQ=255)
                  ggggCGGTACGCAGGGCCAGGGCGAGCATATCGTCGGCCACCCAGTAGCGTGCCTCGACCCGCGCATAGTGGCGGTTCAGCGACGCCAGCGGATGGGCGTGGCGCAGCAGGCCGTCGACATCGGCCTCGCGCAGCCAGCCACGGGCGGCga                                                                                                                             <  1:2445718/151‑1 (MQ=255)
                        gTACGCAGGGCCAGGGCGAGCATATCGTCGGCCACCCAGTAGCGTGCCTCGACCCGCGCATAGTGGCGGTTCAGCGACGCCAGCGGATGGGCGTGGCGCAGCAGGCCGTCGACATCGGCCTCGCGCAGCCAGCCACGGGCGGCGAGACGgc                                                                                                                       <  2:3613105/151‑1 (MQ=255)
                        gTACGCAGGGCCAGGGCGAGCATATCGTCGGCCACCCAGTAGCGTGCCTCGACCCGCGCATAGTGGCGGTTCAGCGACGCCAGCGGATGGGCGTGGCGCAGCAGGCCGTCGACATCGGCCTCGCGCAGCCAGCCACGGGCGGCGAGACGgc                                                                                                                       >  1:10669620/1‑151 (MQ=255)
                            gCAGGGCCAGGGCGAGCATATCGTCGGCCACCCAGTAGCGTGCCTCGACCCGCGCATAGTGGCGGTTCAGCGACGCCAGCGGATGGGCGTGGCGCAGCAGGCCGTCGACATCGGCCTCGCGCAGCCAGCCACGGGCGGCGAGACGGCGCAg                                                                                                                   >  2:11365804/1‑151 (MQ=255)
                            gCAGGGCCAGGGCGAGCATATCGTCGGCCACCCAGTAGCGTGCCTCGACCCGCGCATAGTGGCGGTTCAGCGACGCCAGCGGATGGGCGTGGCGCAGCAGGCCGTCGACATCGGCCTCGCGCAGCCAGCCACGGGCGGCGAGACGGCGCAg                                                                                                                   >  2:1174058/1‑151 (MQ=255)
                              agggccagggcGAGCATATCGTCGGCCACCCAGTAGCGTGCCTCGACCCGCGCATAGTGGCGGTTCAGCGACGCCAGCGGATGGGCGTGGCGCAGCAGGCCGTCGACATCGGCCTCGCGCAGCCAGCCACGGGCGGCGAGACGGCGCAggg                                                                                                                 <  1:760097/151‑1 (MQ=255)
                                ggccagggcGAGCATATCGTCGGCCACCCAGTAGCGTGCCTCGACCCGCGCATAGTGGCGGTTCAGCGACGCCAGCGGATGGGCGTGGCGCAGCAGGCCGTCGACATCGGCCTCGCGCAGCCAGCCACGGGCGGCGAGACGGCGCAGGGGt                                                                                                               >  1:4573281/1‑151 (MQ=255)
                                    agggcGAGCATATCGTCGGCCACCCAGTAGCGTGCCTCGACCCGCGCATAGTGGCGGTTCAGCGACGCCAGCGGATGGGCGTGGCGCAGCAGGCCGTCGACATCGGCCTCGCGCAGCCAGCCACGGGCGGCGAGACGGCGCAGGGGTGCGa                                                                                                           >  2:10386341/1‑151 (MQ=255)
                                     gggcGAGCATATCGTCGGCCACCCAGTAGCGTGCCTCGACCCGCGCATAGTGGCGGTTCAGCGACGCCAGCGGATGGGCGTGGCGCAGCAGGCCGTCGACATCGGCCTCGCGCAGCCAGCCACGGGCGGCGAGACGGCGCAGGGGTGCGAg                                                                                                          <  1:2952147/151‑1 (MQ=255)
                                     gggcGAGCATATCGTCGGCCACCCAGTAGCGTGCCTCGACCCGCGCATAGTGGCGGTTCAGCGACGCCAGCGGATGGGCGTGGCGCAGCAGGCCGTCGACATCGGCCTCGCGCAGCCAGCCACGGGCGGCGAGACGGCGCAGGGGTGCGAg                                                                                                          <  1:2271186/151‑1 (MQ=255)
                                      ggcGAGCATATCGTCGGCCACCCAGTAGCGTGCCTCGACCCGCGCATAGTGGCGGTTCAGCGACGCCAGCGGATGGGCGTGGCGCAGCAGGCCGTCGACATCGGCCTCGCGCAGCCAGCCACGGGCGGCGAGACGGCGCAGGGGTGCGAGc                                                                                                         <  1:304973/151‑1 (MQ=255)
                                       gcGAGCATATCGTCGGCCACCCAGTAGCGTGCCTCGACCCGCGCATAGTGGCGGTTCAGCGACGCCAGCGGATGGGCGTGGCGCAGCAGGCCGTCGACATCGGCCTCGCGCAGCCAGCCACGGGCGGCGAGACGGCGCAGGGGTGCGAGCa                                                                                                        <  1:7824085/151‑1 (MQ=255)
                                        cGAGCATATCGTCGGCCACCCAGTAGCGTGCCTCGACCCGCGCATAGTGGCGGTTCAGCGACGCCAGCGGATGGGCGTGGCGCAGCAGGCCGTCGACATCGGCCTCGCGCAGCCAGCCACGGGCGGCGAGACGGCGCAGGGGTGCGAGCaa                                                                                                       >  1:9987255/1‑151 (MQ=255)
                                         gAGCATATCGTCGGCCACCCAGTAGCGTGCCTCGACCCGCGCATAGTGGCGGTTCAGCGACGCCAGCGGATGGGCGTGGCGCAGCAGGCCGTCGACATCGGCCTCGCGCAGCCAGCCACGGGCGGCGAGACGGCGCAGGGGTGCGAGCaaa                                                                                                      >  2:2356040/1‑151 (MQ=255)
                                         gAGCATATCGTCGGCCACCCAGTAGCGTGCCTCGACCCGCGCATAGTGGCGGTTCAGCGACGCCAGCGGATGGGCGTGGCGCAGCAGGCCGTCGACATCGGCCTCGCGCAGCCAGCCACGGGCGGCGAGACGGCGCAGGGGTGCGAGCaaa                                                                                                      <  1:809831/151‑1 (MQ=255)
                                           gCATATCGTCGGCCACCCAGTAGCGTGCCTCGACCCGCGCATAGTGGCGGTTCAGCGACGCCAGCGGATGGGCGTGGCGCAGCAGGCCGTCGACATCGGCCTCGCGCAGCCAGCCACGGGCGGCGAGACGGCGCAGGGGTGCGAGCAAAgg                                                                                                    <  1:5525332/151‑1 (MQ=255)
                                            cATATCGTCGGCCACCCAGTAGCGTGCCTCGACCCGCGCATAGTGGCGGTTCAGCGACGCCAGCGGATGGGCGTGGCGCAGCAGGCCGTCGACATCGGCCTCGCGCAGCCAGCCACGGGCGGCGAGACGGCGCAGGGGTGCGAGCAAAggg                                                                                                   >  1:9856811/1‑151 (MQ=255)
                                              tatCGTCGGCCACCCAGTAGCGTGCCTCGACCCGCGCATAGTGGCGGTTCAGCGACGCCAGCGGATGGGCGTGGCGCAGCAGGCCGTCGACATCGGCCTCGAGCAGCCAGCCACGGGCGGCGAGACGGCGCAGGGGTGCGAGCAAAGGGGt                                                                                                 <  2:5445385/151‑1 (MQ=255)
                                               atCGTCGGCCACCCAGTAGCGTGCCTCGACCCGCGCATAGTGGCGGTTCAGCGACGCCAGCGGATGGGCGTGGCGCAGCAGGCCGTCGACATCGGCCTCGCGCAGCCAGCCACGGGCGGCGAGACGGCGCAGGGGTGCGAGCAAAGGGGTc                                                                                                >  2:1764151/1‑151 (MQ=255)
                                                   tcgGCCACCCAGTAGCGTGCCTCGACCCGCGCATAGTGGCGGTTCAGCGACGCCAGCGGATGGGCGTGGCGCAGCAGGCCGTCGACATCGGCCTCGCGCAGCCAGCCACGGGCGGCGAGACGGCGCAGGGGTGCGAGCAAAGGGGTCAGga                                                                                            <  1:8811420/151‑1 (MQ=255)
                                                    cgGCCACCCAGTAGCGTGCCTCGACCCGCGCATAGTGGCGGTTCAGCGACGCCAGCGGATGGGCGTGGCGCAGCAGGCCGTCGACATCGGCCTCGCGCAGCCAGCCACGGGCGGCGAGACGGCGCAGGGGTGCGAGCAAAGGGGTCAGgag                                                                                           <  1:5762638/151‑1 (MQ=255)
                                                      gCCACCCAGTAGCGTGCCTCGACCCGCGCATACTGGCGGTTCAGCCACGCCAGCGGCTGGGCGTGGCGCAGCAGGCCGTCGACATCGGCCCCGCGCAGCCAGCCACGGGCGGCGAGACGGCGCAGGGGTGCGAGCAAAGGGGTCAGGagag                                                                                         <  2:8544048/151‑1 (MQ=255)
                                                       ccACCCAGTAGCGTGCCTCGACCCGCGCATAGTGGCGGTTCAGCGACGCCAGCGGATGGGCGTGGCGCAGCAGGCCGTCGACATCGGCCTCGCGCAGCCAGCCACGGGCGGCGAGACGGCGCAGGGGTGCGAGCAAAGGGGTCAGGAGAGc                                                                                        <  2:7812781/151‑1 (MQ=255)
                                                       ccACCCAGTAGCGTGCCTCGACCCGCGCATAGTGGCGGTTCAGCGACGCCAGCGGATGGGCGTGGCGCAGCAGGCCGTCGACATCGGCCTCGCGCAGCCAGCCACGGGCGGCGAGACGGCGCAGGGGTGCGAGCAAAGGGGTCAGGAGAGc                                                                                        >  2:6217072/1‑151 (MQ=255)
                                                          cccAGTAGCGTGCCTCGACCCGCGCATAGTGGCGGTTCAGCGACGCCAGCGGATGGGCGTGGCGCAGCAGGCCGTCGACATCGGCCTCGCGCAGCCAGCCACGGGCGGCGAGACGGCGCAGGGGTGCGAGCAAAGGGGTCAGGAGAGCGAg                                                                                     >  2:4565653/1‑151 (MQ=255)
                                                          cccAGTAGCGTGCCTCGACCCGCGCATAGTGGCGGTTCAGCGACGCCAGCGGATGGGCGTGGCGCAGCAGGCCGTCGACATCGGCCTCGCGCAGCCAGCCACGGGCGGCGAGACGGCGCAGGGGTGCGAGCAAAGGGGTCAGGAGAGCGAg                                                                                     >  2:934678/1‑151 (MQ=255)
                                                          cccAGTAGCGTGCCTCGACCCGCGCATAGTGGCGGTTCAGCGACGCCAGCGGATGGGCGTGGCGCAGCAGGCCGTCGACATCGGCCTCGCGCAGCCAGCCACGGGCGGCGAGACGGCGCAGGGGTGCGAGCAAAGGGGTCAGGAGAGCGAg                                                                                     <  1:6051884/151‑1 (MQ=255)
                                                          cccAGTAGCGTGCCTCGACCCGCGCATAGTGGCGGTTCAGCGACGCCAGCGGATGGGCGTGGCGCAGCAGGCCGTCGACATCGGCCTCGCGCAGCCAGCCACGGGCGGCGAGACGGCGCAGGGGTGCGAGCAAAGGGGTCAGGAGAGCGAg                                                                                     >  2:2873455/1‑151 (MQ=255)
                                                           ccAGTAGCGTGCCTCGACCCGCGCATAGTGGCGGTTCAGCGACGCCAGCGGATGGGCGTGGCGCAGCAGGCCGTCGACATCGGCCTCGCGCAGCCAGCCACGGGCGGCGAGACGGCGCAGGGGTGCGAGCAAAGGGGTCAGGAGAGCGAgg                                                                                    >  1:4852807/1‑151 (MQ=255)
                                                            cAGTAGCGTGCCTCGACCCGCGCATAGTGGCGGTTCAGCGACGCCAGCGGATGGGCGTGGCGCAGCAGGCCGTCGACATCGGCCTCGCGCAGCCAGCCACGGGCGGCGAGACGGCGCAGGGGTGCGAGCAAAGGGGTCAGGAGAGCGAggg                                                                                   <  2:6992713/151‑1 (MQ=255)
                                                             aGTAGCGTGCCTCGACCCGCGCATAGTGGCGGTTCAGCGACGCCAGCGGATGGGCGTGGCGCAGCAGGCCGTCGACATCGGCCTCGCGCAGCCAGCCACGGGCGGCGAGACGGCGCAGGGGTGCGAGCAAAGGGGTCAGGAGAGCGAgggg                                                                                  >  2:7463488/1‑151 (MQ=255)
                                                                aGCGTGCCTCGACCCGCGCATAGTGGCGGTTCAGCGACGCCAGCGGATGGGCGTGGCGCAGCAGGCCGTCGACATCGGCCTCGCGCAGCCAGCCACGGGCGGCGAGACGGCGCAGGGGTGCGAGCAAAGGGGTCAGGAGAGCGAGGGGAAg                                                                               <  2:8485161/151‑1 (MQ=255)
                                                                aGCGTGCCTCGACCCGCGCATAGTGGCGGTTCAGCGACGCCAGCGGATGGGCGTGGCGCAGCAGGCCGTCGACATCGGCCTCGCGCAGCCAGCCACGGGCGGCGAGACGGCGCAGGGGTGCGAGCAAAGGGGTCAGGAGAGCGAGGGGAAg                                                                               >  1:1074402/1‑151 (MQ=255)
                                                                aGCGTGCCTCGACCCGCGCATAGTGGCGGTTCAGCGACGCCAGCGGATGGGCGTGGCGCAGCAGGCCGTCGACATCGGCCTCGCGCAGCCAGCCACGGGCGGCGAGACGGCGCAGGGGTGCGAGCAAAGGGGTCAGGAGAGCGAGGGGAAg                                                                               >  2:2318087/1‑151 (MQ=255)
                                                                 gCGTGCCTCGACCCGCGCATAGTGGCGGTTCAGCGACGCCAGCGGATGGGCGTGGCGCAGCAGGCCGTCGACATCGGCCTCGCGCAGCCAGCCACGGGCGGCGAGACGGCGCAGGGGTGCGAGCAAAGGGGTCAGGAGAGCGCGGGGAAGc                                                                              >  1:7744951/1‑151 (MQ=255)
                                                                  cGTGCCTCGACCCGCGCATAGTGGCGGTTCAGCGACGCCAGCGGATGGGCGTGGCGCAGCAGGCCGTCGACATCGGCCTCGCGCAGCCAGCCACGGGCGGCGAGACGGCGCAGGGGTGCGAGCAAAGGGGTCAGGAGAGCGAGGGGAAGCa                                                                             <  1:1481775/151‑1 (MQ=255)
                                                                    tGCCTCGACCCGCGCATAGTGGCGGTTCAGCGACGCCAGCGGATGGGCGTGGCGCAGCAGGCCGTCGACATCGGCCTCGCGCAGCCAGCCACGGGCGGCGAGACGGCGCAGGGGTGCGAGCAAAGGGGTCAGGAGAGCGAGGGGAAGCAAg                                                                           <  1:5175090/151‑1 (MQ=255)
                                                                     gCCTCGACCCGCGCATAGTGGCGGTTCAGCGACGCCAGCGGATGGGCGTGGCGCAGCAGGCCGTCGACATCGGCCTCGCGCAGCCAGCCACGGGCGGCGAGACGGCGCAGGGGTGCGAGCAAAGGGGTCAGGAGAGCGAGGGGAAGCAAgg                                                                          <  2:675025/151‑1 (MQ=255)
                                                                     gCCTCGACCCGCGCATAGTGGCGGTTCAGCGACGCCAGCGGATGGGCGTGGCGCAGCAGGCCGTCGACATCGGCCTCGCGCAGCCAGCCACGGGCGGCGAGACGGCGCAGGGGTGCGAGCAAAGGGGTCAGGAGAGCGAGGGGAAGCAAgg                                                                          >  2:3261523/1‑151 (MQ=255)
                                                                      ccTCGACCCGCGCATAGTGGCGGTTCAGCGACGCCAGCGGATGGGCGTGGCGCAGCAGGCCGTCGACATCGGCCTCGCGCAGCCAGCCACGGGCGGCGAGACGGCGCAGGGGTGCGAGCAAAGGGGTCAGGAGAGCGAGGGGAAGCAAGGc                                                                         >  2:710757/1‑151 (MQ=255)
                                                                       cTCGACCCGCGCATAGTGGCGGTTCAGCGACGCCAGCGGATGGGCGTGGCGCAGCAGGCCGTCGACATCGGCCTCGCGCAGCCAGCCACGGGCGGCGAGACGGCGCAGGGGTGCGAGCAAAGGGGTCAGGAGAGCGAGGGGAAGCAAGGcc                                                                        <  2:4061368/151‑1 (MQ=255)
                                                                        tCGACCCGCGCATAGTGGCGGTTCAGCGACGCCAGCGGATGGGCGTGGCGCAGCAGGCCGTCGACATCGGCCTCGCGCAGCCAGCCACGGGCGGCGAGACGGCGCAGGGGTGCGAGCAAAGGGGTCAGGAGAGCGAGGGGAAGCAAGGCCa                                                                       <  2:9522355/151‑1 (MQ=255)
                                                                        tCGACCCGCGCATAGTGGCGGTTCAGCGACGCCAGCGGATGGGCGTGGCGCAGCAGGCCGTCGACATCGGCCTCGCGCAGCCAGCCACGGGCGGCGAGACGGCGCAGGGGTGCGAGCAAAGGGGTCAGGAGAGCGAGGGGAAGCAAGGCCa                                                                       <  2:10084826/151‑1 (MQ=255)
                                                                           aCCCGCGCATAGTGGCGGTTCAGCGACGCCAGCGGATGGGCGTGGCGCAGCAGGCCGTCGACATCGGCCTCGCGCAGCCAGCCACGGGCGGCGAGACGGCGCAGGGGTGCGAGCAAAGGGGTCAGGAGAGCGAGGGGAAGCAAGGCCATCg                                                                    <  2:10487917/151‑1 (MQ=255)
                                                                           aCCCGCGCATAGTGGCGGTTCAGCGACGCCAGCGGATGGGCGTGGCGCAGCAGGCCGTCGACATCGGCCTCGCGCAGCCAGCCACGGGCGGCGAGACGGCGCAGGGGTGCGAGCAAAGGGGTCAGGAGAGCGAGGGGAAGCAAGGCCATCg                                                                    <  1:4272194/151‑1 (MQ=255)
                                                                           aCCCGCGCATAGTGGCGGTTCAGCGACGCCAGCGGATGGGCGTGGCGCAGCAGGCCGTCGACATCGGCCTCGCGCAGCCAGCCACGGGCGGCGAGACGGCGCAGGGGTGCGAGCAAAGGGGTCAGGAGAGCGAGGGGAAGCAAGGCCATCg                                                                    >  1:1281242/1‑151 (MQ=255)
                                                                            cccGCGCTTAGTGGCGGTTCAGCGACGCCAGCGGATGGGCGTGGCGCAGCAGGCCGTCGACATCGGCCTCGCGCAGCCAGCCACGGGCGGCGAGACGGCGCAGGGGTGCGAGCAAAGGGGTCAGGAGAGCGAGGGGAAGCAAGGCCATCga                                                                   <  2:10958995/151‑1 (MQ=255)
                                                                              cgcgCATAGTGGCGGTTCAGCGACGCCAGCGGATGGGCGTGGCGCAGCAGGCCGTCGACATCGGCCTCGCGCAGCCAGCCACGGGCGGCGAGACGGCGCAGGGGTGCGAGCAAAGGGGTCAGGAGAGCGAGGGGAAGCAAGGCCATCgaga                                                                 >  2:4280769/1‑151 (MQ=255)
                                                                              cgcgCATAGTGGCGGTTCAGCGACGCCAGCGGATGGGCGTGGCGCAGCAGGCCGTCGACATCGGCCTCGCGCAGCCAGCCACGGGCGGCGAGACGGCGCAGGGGTGCGAGCAAAGGGGTCAGGAGAGCGAGGGGAAGCAAGGCCATCgaga                                                                 <  1:9219753/151‑1 (MQ=255)
                                                                                cgcATAGTGGCGGTTCAGCGACGCCAGCGGATGGGCGTGGCGCAGCAGGCCGTCGACATCGGCCTCGCGCAGCCAGCCACGGGCGGCGAGACGGCGCAGGGGTGCGAGCAAAGGGGTCAGGAGAGCGAGGGGAAGCAAGGCCATCGAGAat                                                               <  1:6445526/151‑1 (MQ=255)
                                                                                     aGTGGCGGTTCAGCGACGCCAGCGGATGGGCGTGGCGCAGCAGGCCGTCGACATCGGCCTCGCGCAGCCAGCCACGGGCGGCGAGACGGCGCAGGGGTGCGAGCAAAGGGGTCAGGAGAGCGAGGGGAAGCAAGGCCATCGAGAATATTTc                                                          <  2:183578/151‑1 (MQ=255)
                                                                                      gTGGCGGTTCAGCGACGCCAGCGGATGGGCGTGGCGCAGCAGGCCGTCGACATCGGCCTCGCGCAGCCAGCCACGGGCGGCGAGACGGCGCAGGGGTGCGAGCAAAGGGGTCAGGAGAGCGAGGGGAAGCAAGGCCATCGAGAATATTTCa                                                         <  2:6559399/151‑1 (MQ=255)
                                                                                      gTGGCGGTTCAGCGACGCCAGCGGATGGGCGTGGCGCAGCAGGCCGTCGACATCGGCCTCGCGCAGCCAGCCACGGGCGGCGAGACGGCGCAGGGGTGCGAGCAAAGGGGTCAGGAGAGCGAGGGGAAGCAAGGCCATCGAGAATATTTCa                                                         <  1:3608104/151‑1 (MQ=255)
                                                                                          cGGTTCAGCGACGCCAGCGGATGGGCGTGGCGCAGCAGGCCGTCGACATCGGCCTCGCGCAGCCAGCCACGGGCGGCGAGACGGCGCAGGGGTGCGAGCAAAGGGGTCAGGAGAGCGAGGGGAAGCAAGGCCATCGAGAATATTTCAGTGa                                                     <  2:4946659/151‑1 (MQ=255)
                                                                                           ggTTCAGCGACGCCAGCGGATGGGCGTGGCGCAGCAGGCCGTCGACATCGGCCTCGCGCAGCCAGCCACGGGCGGCGAGACGGCGCAGGGGTGCGAGCAAAGGGGTCAGGAGAGCGAGGGGAAGCAAGGCCATCGAGAATATTTCAGTGaa                                                    >  2:2703386/1‑151 (MQ=255)
                                                                                           ggTTCAGCGACGCCAGCGGATGGGCGTGGCGCAGCAGGCCGTCGACATCGGCCTCGCGCAGCCAGCCACGGGCGGCGAGACGGCGCAGGGGTGCGAGCAAAGGGGTCAGGAGAGCGAGGGGAAGCAAGGCCATCGAGAATATTTCAGTGaa                                                    >  2:3444029/1‑151 (MQ=255)
                                                                                           ggTTCAGCGACGCCAGCGGATGGGCGTGGCGCAGCAGGCCGTCGACATCGGCCTCGCGCAGCCAGCCACGGGCGGCGAGACGGCGCAGGGGTGCGAGCAAAGGGGTCAGGAGAGCGAGGGGAAGCAAGGCCATCGAGAATATTTCAGTGaa                                                    <  1:6642802/151‑1 (MQ=255)
                                                                                               cAGCGACGCCAGCGGATGGGCGTGGCGCAGCAGGCCGTCGACATCGGCCTCGCGCAGCCAGCCACGGGCGGCGAGACGGCGCAGGGGTGCGAGCAAAGGGGTCAGGAGAGCGAGGGGAAGCAAGGCCATCGAGAATATTTCAGTGAACGAt                                                <  1:1742322/151‑1 (MQ=255)
                                                                                               cAGCGACGCCAGCGGATGGGCGTGGCGCAGCAGGCCGTCGACATCGGCCTCGCGCAGCCAGCCACGGGCGGCGAGACGGCGCAGGGGTGCGAGCAAAGGGGTCAGGAGAGCGAGGGGAAGCAAGGCCATCGAGAATATTTCAGTGAACGAt                                                <  2:9178780/151‑1 (MQ=255)
                                                                                                aGCGACGCCAGCGGATGGGCGTGGCGCAGCAGGCCGTCGACATCGGCCTCGCGCAGCCAGCCACGGGCGGCGAGACGGCGCAGGGGTGCGAGCAAAGGGGTCAGGAGAGCGAGGGGAAGCAAGGCCATCGAGAATATTTCAGTGAACGATc                                               >  1:8037457/1‑151 (MQ=255)
                                                                                                 gCGACGCCAGCGGATGGGCGTGGCGCAGCAGGCCGTCGACATCGGCCTCGCGCAGCCAGCCACGGGCGGCGAGACGGCGCAGGGGTGCGAGCACTGGGGTCAGGAGAGCGGGGGGAAGCAAGGCCATCGAGCATATTTCAGTGAACGATCg                                              >  1:8613789/1‑151 (MQ=255)
                                                                                                    aCGCCAGCGGATGGGCGTGGCGCAGCAGGCCGTCGACATCGGCCTCGCGCAGCCAGCCACGGGCGGCGAGACGGCGCAGGGGTGCGAGCAAAGGGGTCAGGAGAGCGAGGGGAAGCAAGGCCATCGAGAATATTTCAGTGAACGATCGTTc                                           <  2:2083393/151‑1 (MQ=255)
                                                                                                     cGCCAGCGGATGGGCGTGGCGCAGCAGGCCGTCGACATCGGCCTCGCGCAGCCAGCCACGGGCGGCGAGACGGCGCAGGGGTGCGAGCAAAGGGGTCAGGAGAGCGAGGGGAAGCAAGGCCATCGAGAATATTTCAGTGAACGATCGTTca                                          >  2:5983478/1‑151 (MQ=255)
                                                                                                       cccgCGGATGGGCGTGGCGCAGCAGGCCGTCGACATCGGCCTCGCGCAGCCAGCCACGGGCGGCGAGACGGCGCAGGGGTGCGAGCAAAGGGGTCAGGAGAGCGAGGGGAAGCAAGGCCATCGAGAATATTTCAGTGAACGATCGTGcaca                                        >  2:7333286/4‑151 (MQ=255)
                                                                                                         aGCGGATGGGCGTGGCGCAGCAGGCCGTCGACATCGGCCTCGCGCAGCCAGCCACGGGCGGCGAGACGGCGCAGGGGTGCGAGCAAAGGGGTCAGGAGAGCGAGGGGAAGCAAGGCCATCGAGAATATTTCAGTGAACGATCGTTCACaaa                                      >  1:95973/1‑151 (MQ=255)
                                                                                                         aGCGGATGGGCGTGGCGCAGCAGGCCGTCGACATCGGCCTCGCGCAGCCAGCCACGGGCGGCGAGACGGCGCAGGGGTGCGAGCAAAGGGGTCAGGAGAGCGAGGGGAAGCAAGGCCATCGAGAATATTTCAGTGAACGATCGTTCACaaa                                      <  1:1008393/151‑1 (MQ=255)
                                                                                                          gCGGATGGGCGTGGCGCAGCAGGCCGTCGACATCGGCCTCGCGCAGCCAGCCACGGGCGGCGAGACGGCGCAGGGGTGCGAGCAAAGGGGTCAGGAGAGCGAGGGGAAGCAAGGCCATCGAGAATATTTCAGTGAACGATCGTTCACaaat                                     >  2:11263987/1‑150 (MQ=255)
                                                                                                          gCGGATGGGCGTGGCGCAGCAGGCCGTCGACATCGGCCTCGCGCAGCCAGCCACGGGCGGCGAGACGGCGCAGGGGTGCGAGCAAAGGGGTCAGGAGAGCGAGGGGAAGCAAGGCCATCGAGAATATTTCAGTGAACGATCGTTCACaaaa                                     >  2:5227668/1‑151 (MQ=255)
                                                                                                           cGGATGGGCGTGGCGCAGCAGGCCGTCGACATCGGCCTCGCGCAGCCAGCCACGGGCGGCGAGACGGCGCAGGGGTGCGAGCAAAGGGGTCAGGAGAGCGAGGGGAAGCAAGGCCATCGAGAATATTTCAGTGAACGATCGTTCACAAAAc                                    >  2:3779020/1‑151 (MQ=255)
                                                                                                           cGGATGGGCGTGGCGCAGCAGGCCGTCGACATCGGCCTCGCGCAGCCAGCCACGGGCGGCGAGACGGCGCAGGGGTGCGAGCAAAGGGGTCAGGAGAGCGAGGGGAAGCAAGGCCATCGAGAATATTTCAGTGAACGATCGTTCACAAAAc                                    <  2:8300625/151‑1 (MQ=255)
                                                                                                              aTGGGCGTGGCGCAGCAGGCCGTCGACATCGGCCTCGCGCAGCCAGCCACGGGCGGCGAGACGGCGCAGGGGTGCGAGCAAAGGGGTCAGGAGAGCGAGGGGAAGCAAGGCCATCGAGAATATTTCAGTGAACGATCGTTCACAAAACttt                                 >  1:9561540/1‑151 (MQ=255)
                                                                                                                 ggCGTGGCGCAGCAGGCCGTCGACATCGGCCTCGCGCAGCCAGCCACGGGCGGCGAGACGGCGCAGGGGTGCGAGCAAAGGGGTCAGGAGAGCGAGGGGAAGCAAGGCCATCGAGAATATTTCAGTGAACGATCGTTCACAAAACTTTGcc                              >  2:8676894/1‑151 (MQ=255)
                                                                                                                    gTGGCGCAGCAGGCCGTCGACATCGGCCTCGCGCAGCCAGCCACGGGCGGCGAGACGGCGCAGGGGTGCGAGCAAAGGGGTCAGGAGAGCGAGGGGAAGCAAGGCCATCGAGAATATTTCAGTGAACGATCGGTCACAAATCTTTGCCCGt                           >  1:10937529/1‑151 (MQ=255)
                                                                                                                        cgcAGCAGGCCTTCGACATCGGCCTCGCGCAGCCAGCCACGGGCGGCGAGACGGCGCAGGGGTGCGAGCAAAGGGGTCAGGAGAGCGAGGGGAAGCAAGGCCATCGAGAATATTTCAGTGAACGATCGTTCACAAAACTTTGCCCGTTCcg                       <  1:10302245/151‑1 (MQ=255)
                                                                                                                        cgcAGCAGGCCGTCGACATCGGCCTCGCGCAGCCAGCCACGGGCGGCGAGACGGCGCAGGGGTGCGAGCAAAGGGGTCAGGAGAGCGAGGGGAAGCAAGGCCATCGAGAATATTTCAGTGAACGATCGTTCACAAAACTTTGCCCGTTCcg                       <  1:9356565/151‑1 (MQ=255)
                                                                                                                         gcagcaGGCCGTCGACATCGGCCTCGCGCAGCCAGCCACGGGCGGCGAGACGGCGCAGGGGTGCGAGCAAAGGGGTCAGGAGAGCGAGGGGAAGCAAGGCCATCGAGAATATTTCAGTGAACGATCGTTCACAAAACTTTGCCCGTTCcgc                      <  1:2146190/151‑1 (MQ=255)
                                                                                                                              agGCCGTCGACATCGGCCTCGCGCAGCCAGCCACGGGCGGCGAGACGGCGCAGGGGTGCGAGCAAAGGGGTCAGGAGAGCGAGGGGAAGCAAGGCCATCGAGAATATTTCAGTGAACGATCGTTCACAAAACTTTGCCCGTTCCGCGCAAt                 >  1:3513275/1‑151 (MQ=255)
                                                                                                                              agGCCGTCGACATCGGCCTCGCGCAGCCAGCCACGGGCGGCGAGACGGCGCAGGGGTGCGAGCAAAGGGGTCAGGAGAGCGAGGGGAAGCAAGGCCATCGAGAATATTTCAGTGAACGATCGTTCACAAAACTTTGCCCGTTCCGCGCAAt                 <  1:1835507/151‑1 (MQ=255)
                                                                                                                               ggCCGTCGACATCGGCCTCGCGCAGCCAGCCACGGGCGGCGAGACGGCGCAGGGGTGCGAGCAAAGGGGTCAGGAGAGCGAGGGGAAGCAAGGCCATCGAGAATATTTCAGTGAACGATCGTTCACAAAACTTTGCCCGTTCCGCGCAATc                <  1:11121798/151‑1 (MQ=255)
                                                                                                                                   gTCGACATCGGCCTCGCGCAGCCAGCCACGGGCGGCGAGACGGCGCAGGGGTGCGAGCAAAGGGGTCAGGAGAGCGAGGGGAAGCAAGGCCATCGAGAATATTTCAGTGAACGATCGTTCACAAAACTTTGCCCGTTCCGCGCAATCGAGt            >  2:574422/1‑151 (MQ=255)
                                                                                                                                    tCGACATCGGCCTCGCGCAGCCAGCCACGGGCGGCGAGACGGCGCAGGGGTGCGAGCAAAGGGGTCAGGAGAGCGAGGGGAAGCAAGGCCATCGAGAATATTTCAGTGAACGATCGTTCACAAAACTTTGCCCGTTCCGCGCAATCGAGTc           <  2:3323993/151‑1 (MQ=255)
                                                                                                                                      gACATCGGCCTCGCGCAGCCAGCCACGGGCGGCGAGACGGCGCAGGGGTGCGAGCACAGGGGTCAGGAGAGCGAGGGGAAGCAAGGCCATCGAGAATATTTCAGTGAACGATCGTTCACTAAACTTTGCCCGTTCCGCGCAATCGAGTcaa         >  1:7212943/1‑151 (MQ=255)
                                                                                                                                      gACATCGGCCTCGCGCAGCCAGCCACGGGCGGCGAGACGGCGCAGGGGTGCGAGCAAAGGGGTCAGGAGAGCGAGGGGACGCAAGGCCATCGAGAATATTTCAGTGAACGATCGTTCACAAAACTTTGCCCGTTCCGCGCAATCGAGTcaa         >  2:1598977/1‑151 (MQ=255)
                                                                                                                                       tcATCGGCCTCGCGCAGCCAGCCACGGGCGGCGAGACGGCGCAGGGGTGCGAGCAAAGGGGTCAGGAGAGCGAGGGGAAGCAAGGCCATCGAGAATATTTCAGTGAACGATCGTTCACAAAACTTTGCCCGTTCCGCGCAATCGAGTcaac        >  1:1127952/2‑151 (MQ=255)
                                                                                                                                        cATCGGCCTCGCGCAGCCAGCCACGGGCGGCGAGACGGCGCAGGGGTGCGAGCAAAGGGGTCAGGAGAGCGAGGGGAAGCAAGGCCATCGAGAATATTTCAGTGAACGATCGTTCACAAAACTTTGCCCGTTCCGCGCAATCGAGTcaaca       >  1:8289485/1‑151 (MQ=255)
                                                                                                                                          tCGGCCTCGCGCAGCCAGCCACGGGCGGCGAGACGGCGCAGGGGTGCGAGCAAAGGGGTCAGGAGAGCGAGGGGAAGCAAGGCCATCGAGAATATTTCAGTGAACGATCGTTCACAAAACTTTGCCCGTTCCGCGCAATCGAGTCAACAAg     >  2:3061601/1‑151 (MQ=255)
                                                                                                                                          tCGGCCTCGCGCAGCCAGCCACGGGCGGCGAGACGGCGCAGGGGTGCGAGCAAAGGGGTCAGGAGAGCGAGGGGAAGCAAGGCCATCGAGAATATTTCAGTGAACGATCGTTCACAAAACTTTGCCCGTTCCGCGCAATCGAGTCAACAAg     >  2:7250031/1‑151 (MQ=255)
                                                                                                                                           cGGCCTCGCGCAGCCAGCCACGGGCGGCGAGACGGCGCAGGGGTGCGAGCAAAGGGGTCAGGAGAGCGAGGGGAAGCAAGGCCATCGAGAATATTTCAGTGAACGATCGTTCACAAAACTTTGCCCGTTCCGCGCAATCGAGTCAACAAGc    >  1:4684024/1‑151 (MQ=255)
                                                                                                                                            ggCCTCGCGCAGCCCGCCACGGGCGGCGAGACGGCGCAGGGGTGCGAGCAAAGGGGTCAGGAGAGCGAGGGGAAGCAAGGCCATCGAGAATATTTCAGTGAACGATCGTTCACAAAACTTTGCCCGTTCCGCGCAATCGAGTCAACAAGCg   <  2:10679134/151‑1 (MQ=255)
                                                                                                                                            ggCCTCGCGCAGCCAGCCACGGGCGGCGAGACGGCGCAGGGGTGCGAGCAAAGGGGTCAGGAGAGCGAGGGGAAGCAAGGCCATCGAGAATATTTCAGTGAACGATCGTTCACAAAACTTTGCCCGTTCCGCGCACTCGAGTCAACAAGCg   >  2:1460617/1‑151 (MQ=255)
                                                                                                                                            ggCCTCGCGCAGCCAGCCACGGGCGGCGAGACGGCGCAGGGGTGCGAGCAAAGGGGTCAGGAGAGCGAGGGGAAGCAAGGCCATCGAGAATATTTCAGTGAACGATCGTTCACAAAACTTTGCCCGTTCCGCGCAATCGAGTCAACAAGCg   <  1:3916035/151‑1 (MQ=255)
                                                                                                                                             gCCTCGCGCAGCCAGCCACGGGCGGCGAGACGGCGCAGGGGTGCGAGCAAAGGGGTCAGGAGAGCGAGGGGAAGCAAGGCCATCGAGAATATTTCAGTGAACGATCGTTCACAAAACTTTGCCCGTTCCGCGCAATCGAGTCAACAAGCGt  <  1:10441793/151‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
CGCGCCGGCGGCCAGCCGGGGGCGGTACGCAGGGCCAGGGCGAGCATATCGTCGGCCACCCAGTAGCGTGCCTCGACCCGCGCATAGTGGCGGTTCAGCGACGCCAGCGGATGGGCGTGGCGCAGCAGGCCGTCGACATCGGCCTGGGGCAGCCAGCCACGGGCGGCGAGACGGCGCAGGGGTGCGAGCAAAGGGGTCAGGAGAGCGAGGGGAAGCAAGGCCATCGAGAATATTTCAGTGAACGATCGTTCACAAAACTTTGCCCGTTCCGCGCAATCGAGTCAACAAGCGT  >  NC_008463/5757380‑5757671

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑