Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 1,375,947 A→G intergenic (+18/‑38) PA14_RS06470 → / → PA14_RS06475 PspA/IM30 family protein/DUF1449 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084631,375,9470AG99.8% 1902.1 / NA 482intergenic (+18/‑38)PA14_RS06470/PA14_RS06475PspA/IM30 family protein/DUF1449 family protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (217/264);  minor base T (1/0);  total (218/264)
Fisher's exact test for biased strand distribution p-value = 4.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 483 total aligned reads displayed.

CGGCCGAACTGGCCAGCGCGGCGGCGCCGGACGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCAAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGATCATCTACTGGGGCATCGTCGCCCTGGGCATGGTCGAGAT  >  NC_008463/1375799‑1376094
                                                                                                                                                    |                                                                                                                                                   
 ggCCGAACTGGCCAGCGCGGCGGCGCCGGACGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAg                                                                                                                                                  <  1:2552243/151‑1 (MQ=255)
   ccGAACTGGCCAGCGCGGCGGCGCCGGACGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGc                                                                                                                                                <  1:8236817/151‑1 (MQ=255)
   ccGAACTGGCCAGCGCGGCGGCGCCGGACGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGc                                                                                                                                                <  1:2683162/151‑1 (MQ=255)
   ccGAACTGGCCAGCGCGGCGGCGCCGGACGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGc                                                                                                                                                <  1:8892354/151‑1 (MQ=255)
    cGAACTGGCCAGCGCGGCGGCGCCGGACGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCg                                                                                                                                               >  2:8780478/1‑151 (MQ=255)
      aaCTGGCCAGCGCGGCGGCGCCGGACGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTc                                                                                                                                             <  2:2998592/151‑1 (MQ=255)
       aCTGGCCAGCGCGGCGGCGCCGGACGATGCGCTGGACGCCACGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTcc                                                                                                                                            <  1:4379998/151‑1 (MQ=255)
        cTGGCCAGCGCGGCGGCGCCGGACGATGCGCTGGTCGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCg                                                                                                                                           <  2:10424260/151‑1 (MQ=255)
        cTGGCCAGCGCGGCGGCGCCGGACGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCg                                                                                                                                           <  2:324993/151‑1 (MQ=255)
        cTGGCCAGCGCGGCGGCGCCGGACGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCg                                                                                                                                           <  2:5828876/151‑1 (MQ=255)
         tGGCCAGCGCGGCGGCGCCGGACGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGCTGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCgg                                                                                                                                          <  2:3582018/151‑1 (MQ=255)
          ggCCAGCGCGGCGGCGCCGGACGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGACGGCGTCCggg                                                                                                                                         >  1:710265/1‑151 (MQ=255)
          ggCCAGCGCGGCGGCGCCGGACGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCggg                                                                                                                                         >  1:11559797/1‑151 (MQ=255)
           gCCAGCGCGGCGGCGCCGGACGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGc                                                                                                                                        <  2:3363323/151‑1 (MQ=255)
            ccAGCGCGGCGGCGCCGGACGATGCGCTGGACGCCAAGCTGAGCGCGGCCTGGATCAAGGCCTCGGGCAACATCGTGGATGGCGTGCTGGCGCGCCTGAAGGCAAAGGGCAAGGCGTGGTGGGCCCGCGGCCCGGCGAGGGCGGCGGGGcc                                                                                                                                       >  2:3179850/1‑151 (MQ=255)
                 gcggcggcgCCGGACGATGCGCTGGACGCCACGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGttt                                                                                                                                  <  2:7095902/151‑1 (MQ=255)
                 gcggcggcgCCGGACGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGttt                                                                                                                                  <  2:389058/151‑1 (MQ=255)
                 gcggcggcgCCGGACGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGttt                                                                                                                                  <  1:9089973/151‑1 (MQ=255)
                  cggcggcgCCGGACGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGtttt                                                                                                                                 <  1:4833803/151‑1 (MQ=255)
                    gcggcgCCGGACGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGtttttg                                                                                                                               <  1:7243573/151‑2 (MQ=255)
                    gcggcgCCGGACGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTc                                                                                                                               <  1:8596593/151‑1 (MQ=255)
                    gcggcgCCGGACGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTc                                                                                                                               <  1:525150/151‑1 (MQ=255)
                      ggcgCCGGACGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAg                                                                                                                             >  1:7476915/1‑151 (MQ=255)
                       gcgcCGGACGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAgg                                                                                                                            >  2:808896/1‑151 (MQ=255)
                       gcgcCGGACGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAgg                                                                                                                            <  1:7692992/151‑1 (MQ=255)
                         gcCGGACGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAgggg                                                                                                                          >  1:6228939/1‑150 (MQ=255)
                         gcCGGACGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGa                                                                                                                          >  1:5593661/1‑151 (MQ=255)
                         gcCGGACGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGa                                                                                                                          >  1:10754686/1‑151 (MQ=255)
                          ccGGACGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGAt                                                                                                                         <  1:6662845/151‑1 (MQ=255)
                          ccGGACGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGAt                                                                                                                         >  2:2655964/1‑151 (MQ=255)
                           cGGACGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCCAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATa                                                                                                                        >  1:2261554/1‑151 (MQ=255)
                           cGGACGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGACGGCGTCCGGGCCCGTTTTTCAGGGATa                                                                                                                        <  2:1452239/151‑1 (MQ=255)
                            ggACGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATag                                                                                                                       <  2:8339631/151‑1 (MQ=255)
                              aCGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATagag                                                                                                                     <  2:6274643/151‑1 (MQ=255)
                               cGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGTCCTCGGGCAACAGCGTGGATGGCGTGATGGCGCGCGTGAAGGAAAAGGGCAAGGCCTGATAGTCCCGCGGCCCGGCGAAGGCGTCCGGGCCGGTGTTTCTGGGATAGAGc                                                                                                                    >  2:992669/1‑151 (MQ=255)
                               cGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGGCCGGGCCCGTTTTTCAGGGATAGAGc                                                                                                                    >  2:11225197/1‑151 (MQ=255)
                                   gcgcTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACa                                                                                                                <  2:520992/151‑1 (MQ=255)
                                     gcTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACCGCGTGGATGGCGTGCTGGCGCGCCGGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATCGAGCGACact                                                                                                              >  2:4078925/1‑149 (MQ=255)
                                     gcTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAAt                                                                                                              >  2:11586345/1‑151 (MQ=255)
                                       tGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCACCAGCGTGGCTGGCGTGCTGGCGCGCCTGACGGAAAAGGGCAAGGCCGGATAGGCCCGCGGCCCGGCGAAGGCGGCCGGGCCCGGTTTTCAGGGATAGAGCGACAATgg                                                                                                            >  1:11423211/1‑151 (MQ=255)
                                        ggACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGa                                                                                                           <  1:4706723/151‑1 (MQ=255)
                                          aCGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAg                                                                                                         <  1:7923265/151‑1 (MQ=255)
                                           cGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGATATGGGCAAGGCGTGAGAGGCCCGCGGCCCGGCGTAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACTATGGAAgg                                                                                                        >  2:10009419/1‑151 (MQ=255)
                                           cGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAgg                                                                                                        >  1:8184160/1‑151 (MQ=255)
                                              cAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGttt                                                                                                     <  2:6487722/151‑1 (MQ=255)
                                                  cTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTg                                                                                                 <  1:1350608/151‑1 (MQ=255)
                                                   tGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGc                                                                                                <  2:8558205/151‑1 (MQ=255)
                                                     cgcgcgGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAg                                                                                              <  1:9583352/151‑1 (MQ=255)
                                                        gcgGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAACAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTATTTCAGGGATAGAGCGACAATGGACGGGTTCCTGCAGACg                                                                                           <  1:10458478/151‑1 (MQ=255)
                                                        gcgGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACg                                                                                           <  2:1694732/151‑1 (MQ=255)
                                                            ccGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGgcgc                                                                                       >  1:3054639/1‑151 (MQ=255)
                                                                gATCAAGGCCTCGGGCAACAGCGTGGCAGGCGTGCGGGCGCGCCTGACGGACAAGGGCAAGGCCTGCTAGGCCCGCGGCCCGGCGACGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGGTTCCTGCAGACGGCGCTGTc                                                                                   <  2:1102826/151‑1 (MQ=255)
                                                                gATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTc                                                                                   <  2:9287557/151‑1 (MQ=255)
                                                                gATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTc                                                                                   <  1:5894989/151‑1 (MQ=255)
                                                                 aTCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCg                                                                                  <  2:6014225/151‑1 (MQ=255)
                                                                    aaGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGttt                                                                               <  1:11045569/151‑1 (MQ=255)
                                                                      ggCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTcc                                                                             <  1:331783/151‑1 (MQ=255)
                                                                        ccTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGACCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGa                                                                           >  1:11224038/1‑151 (MQ=255)
                                                                               cAACAGCTTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGTCCCGTTATTCAGGGATAGAGCGACAAGGGAAGGTGTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCt                                                                    >  2:8649672/1‑151 (MQ=255)
                                                                               cAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCt                                                                    <  2:7941044/151‑1 (MQ=255)
                                                                               cAACAGCGGGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCt                                                                    >  1:3527697/1‑151 (MQ=255)
                                                                                 aCAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGt                                                                  <  1:206020/151‑1 (MQ=255)
                                                                                   aGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTc                                                                >  1:366836/1‑151 (MQ=255)
                                                                                    gCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCa                                                               <  1:9238010/151‑1 (MQ=255)
                                                                                     cGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAg                                                              >  1:6728114/1‑151 (MQ=255)
                                                                                        ggATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCtt                                                           <  2:9179152/151‑1 (MQ=255)
                                                                                            ggCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTttctt                                                       >  2:8996529/1‑151 (MQ=255)
                                                                                                 gCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGAt                                                  >  2:6863189/1‑151 (MQ=255)
                                                                                                     gcgcgcCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTc                                              <  2:5467174/151‑1 (MQ=255)
                                                                                                     gcgcgcCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTc                                              <  1:5740525/151‑1 (MQ=255)
                                                                                                     gcgcgcCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTc                                              <  2:4114292/151‑1 (MQ=255)
                                                                                                     gcgcgcCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTGTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTc                                              <  2:4573225/151‑1 (MQ=255)
                                                                                                      cgcgcCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTcg                                             <  1:3988990/151‑1 (MQ=255)
                                                                                                       gcgcCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTcgc                                            <  1:4570855/151‑1 (MQ=255)
                                                                                                       gcgcCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTcgc                                            <  1:9783473/151‑1 (MQ=255)
                                                                                                        cgcCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTcgcg                                           <  1:5051246/151‑1 (MQ=255)
                                                                                                          ccTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGat                                         <  1:10653642/151‑1 (MQ=255)
                                                                                                             gAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGatcat                                      >  2:7479913/1‑151 (MQ=255)
                                                                                                             gAAGGAAAAGGGCAAGGCCTGATAGGCCCGAGGCCCGGCTAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAAAGGAAGGTTTCCTGCAGACGGCGAAGTCGTTTCAGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGatcat                                      >  2:3748193/1‑151 (MQ=255)
                                                                                                              aaGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGatcatc                                     >  1:6076959/1‑151 (MQ=255)
                                                                                                                 gAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGATCATCTAc                                  >  2:1324109/1‑151 (MQ=255)
                                                                                                                 gAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGATCATCTAc                                  >  2:10407568/1‑151 (MQ=255)
                                                                                                                    aaGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGATCATCTACTgg                               >  1:8356276/1‑151 (MQ=255)
                                                                                                                     aGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGATCATCTACTggg                              <  2:1856657/151‑1 (MQ=255)
                                                                                                                       ggCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGATCATCTACTGGGGc                            <  2:11056744/151‑1 (MQ=255)
                                                                                                                          aaGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGATCATCTACTGGGGCAtc                         <  2:10258025/151‑1 (MQ=255)
                                                                                                                            ggCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGATCATCTACTGGGGCAtcgt                       <  1:10034689/151‑1 (MQ=255)
                                                                                                                            ggCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGATCATCTACTGGGGCAtcga                       >  2:10608931/1‑150 (MQ=255)
                                                                                                                             gCCGGATAGGCCCGCGGCCCGGCGACGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGATCATCTACTGGGGCAtcgtc                      <  2:6617056/151‑1 (MQ=255)
                                                                                                                              ccTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGATCATCTACTGGGGCAtcgtcg                     <  1:8734151/151‑1 (MQ=255)
                                                                                                                              ccTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGATCATCTACTGGGGCAtcgtcg                     <  2:4373436/151‑1 (MQ=255)
                                                                                                                               cTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGATCATCTACTGGGGCATCGTCGc                    <  1:4213295/151‑1 (MQ=255)
                                                                                                                                tGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGATCATCTACTGGGGCATCGTCGcc                   >  1:1822174/1‑151 (MQ=255)
                                                                                                                                 gATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGAGCATCTACTGGGGCATCGTCGccc                  >  2:2890693/1‑151 (MQ=255)
                                                                                                                                    aGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGATCATCTACTGGGGCATCGTCGCCCTgg               <  1:6308549/151‑1 (MQ=255)
                                                                                                                                      gCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGATCATCTACTGGGGCATCGTCGCCCTGGGc             <  1:3838807/151‑1 (MQ=255)
                                                                                                                                        ccGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGATCATCTACTGGGGCATCGTCGCCCTGGGCAt           <  1:9341628/151‑1 (MQ=255)
                                                                                                                                           cgGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGATCATCTACTGGGGCATCGTCGCCCTGGGCATGGt        >  1:10994119/1‑151 (MQ=255)
                                                                                                                                              cccGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGATCATCTACTGGGGCATCGTCGCCCTGGGCATGGTCga     >  2:9631773/1‑151 (MQ=255)
                                                                                                                                              cccGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGATCATCTACTGGGGCATCGTCGCCCTGGGCATGGTCga     <  1:9502480/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CGGCCGAACTGGCCAGCGCGGCGGCGCCGGACGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCAAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGATCATCTACTGGGGCATCGTCGCCCTGGGCATGGTCGAGAT  >  NC_008463/1375799‑1376094

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑