BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_008463	2,149,425	T→C	Q549Q (CAA→CAG)	PA14_RS09965 ←	M14 family metallopeptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_008463	2,149,425	0	T	C	100.0%	1790.4 / NA	461	Q549Q (CAA→CAG)	PA14_RS09965	M14 family metallopeptidase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (224/237); total (224/237)

Only 100 of 461 total aligned reads displayed.

CGCGCAGGCGTTTGTCGAAGGCGGACTTCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTTTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCTGACCAGCAGGCGACCTTCGTAGCCCGGAATCCGGTTG  >  NC_008463/2149277‑2149572

 gcgcAGGCGTTTGTCGAAGGCGGACTTCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGc                                                                                                                                                  >  2:2871590/1‑151 (MQ=255)

    cAGGCGTTTGTCGAAGGCGGACTTCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGt                                                                                                                                               >  2:8478980/1‑151 (MQ=255)

         gTTTGTCGAAGGCGGACTTCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTgcag                                                                                                                                          >  2:2117340/1‑151 (MQ=255)

             gTCGAAGGCGGACTTCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAgg                                                                                                                                      >  1:4260565/1‑151 (MQ=255)

              tCGAAGGCTGACTTCAGCGCAGGGTCTTCCGCCAGAATCTTGCGCGCCATCGGCTCCATCACGTTGTCCTACGGCTCCTCGTCGGCCACCAGGGTGTAATTGAAGAAGCCCCAGGTCCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGt                                                                                                                                     <  2:1894803/151‑1 (MQ=255)

              tCGAAGGCGGACTTCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGt                                                                                                                                     <  1:8253031/151‑1 (MQ=255)

               cGAAGGCGGACTTCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCCGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTc                                                                                                                                    <  2:2789031/151‑1 (MQ=255)

               cGAAGGCGGACTTCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTc                                                                                                                                    >  1:10950276/1‑151 (MQ=255)

               cGAAGGCGGACTTCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTc                                                                                                                                    >  2:2588680/1‑151 (MQ=255)

                 aaGGCGGACTTCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGa                                                                                                                                  >  1:623496/1‑151 (MQ=255)

                 aaGGCGGACTTCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGa                                                                                                                                  >  1:1176415/1‑151 (MQ=255)

                 aaGGCGGACTTCAGCGCCGGGTCTCCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCTGGTCGa                                                                                                                                  >  2:10756842/1‑151 (MQ=255)

                  aGGCGGACTTCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGAt                                                                                                                                 >  2:668019/1‑151 (MQ=255)

                   ggCGGACTTCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATg                                                                                                                                >  2:9765608/1‑151 (MQ=255)

                     cGGACTTCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCCCCAGGGTGGACTTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGc                                                                                                                              >  2:7459494/1‑151 (MQ=255)

                       gACTTCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCa                                                                                                                            <  2:5320578/151‑1 (MQ=255)

                       gACTTCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCa                                                                                                                            >  1:9411399/1‑151 (MQ=255)

                        aCTTCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAg                                                                                                                           >  1:2378323/1‑151 (MQ=255)

                        aCTTCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAg                                                                                                                           <  1:5929518/151‑1 (MQ=255)

                         cTTCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGACTTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAgg                                                                                                                          >  2:11454861/1‑151 (MQ=255)

                           tCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGAc                                                                                                                        <  2:11330882/151‑1 (MQ=255)

                           tCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGAc                                                                                                                        <  1:7546286/151‑1 (MQ=255)

                             aGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGc                                                                                                                      >  2:234920/1‑151 (MQ=255)

                                gcCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCa                                                                                                                   >  1:1793433/1‑151 (MQ=255)

                                gcCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCa                                                                                                                   >  1:1335811/1‑151 (MQ=255)

                                 ccGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGACGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAg                                                                                                                  >  1:7830727/1‑151 (MQ=255)

                                     gTCTTCCGCCATCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGc                                                                                                              <  2:869757/151‑1 (MQ=255)

                                      tCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCt                                                                                                             <  1:3162920/151‑1 (MQ=255)

                                        ttCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTgg                                                                                                           <  2:7679794/151‑1 (MQ=255)

                                         tCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTggg                                                                                                          <  1:1898451/151‑1 (MQ=255)

                                                 cATCTAGCGCGCCAACGGCTCCATCACGTATTCCTCCGGCTCCCCGGCGGCCACCACGGTGGAATTGCAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGtcg                                                                                                  <  1:1733116/151‑1 (MQ=255)

                                                       gcgcgcCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGAc                                                                                            <  2:6851659/151‑1 (MQ=255)

                                                        cgcgcCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACa                                                                                           <  1:9307394/151‑1 (MQ=255)

                                                         gcgcCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGACGAAGCCCCAGGACCAGACCGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAg                                                                                          <  1:10622246/151‑1 (MQ=255)

                                                           gcCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCg                                                                                        >  1:8349943/1‑151 (MQ=255)

                                                           gcCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCg                                                                                        <  1:10341969/151‑1 (MQ=255)

                                                            ccATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGTATTGATGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCCGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGa                                                                                       <  2:7659748/151‑1 (MQ=255)

                                                            ccAACGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGa                                                                                       <  1:887830/151‑1 (MQ=255)

                                                             cATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAg                                                                                      >  2:8675723/1‑151 (MQ=255)

                                                               tCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGGCGATCGACAGCGAgcc                                                                                    <  2:811243/151‑1 (MQ=255)

                                                                 ggCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAgccgg                                                                                  >  1:10005427/1‑151 (MQ=255)

                                                                      cATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCggg                                                                             <  2:1401455/151‑1 (MQ=255)

                                                                       aTCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGa                                                                            >  2:10523251/1‑151 (MQ=255)

                                                                        tCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGaa                                                                           >  2:372574/1‑151 (MQ=255)

                                                                        acACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGaa                                                                           <  1:398699/150‑1 (MQ=255)

                                                                         cACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAg                                                                          <  1:7451141/151‑1 (MQ=255)

                                                                           cGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGt                                                                        <  2:5049705/151‑1 (MQ=255)

                                                                                  ctccGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAg                                                                 <  1:6631505/151‑1 (MQ=255)

                                                                                  ctccGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAg                                                                 <  2:8313679/151‑1 (MQ=255)

                                                                                    ccGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGCACGAATCCGGACTCGGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAgcg                                                               <  2:33324/151‑1 (MQ=255)

                                                                                    ccGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAgcg                                                               <  2:10137516/151‑1 (MQ=255)

                                                                                    ccGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAgcg                                                               >  1:11261433/1‑151 (MQ=255)

                                                                                    ccGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAgcg                                                               <  2:4455328/151‑1 (MQ=255)

                                                                                       gCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTg                                                            <  2:10350577/151‑1 (MQ=255)

                                                                                       gCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTg                                                            >  1:6849931/1‑151 (MQ=255)

                                                                                       gCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTg                                                            <  1:251145/151‑1 (MQ=255)

                                                                                        ctcctcGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGACCGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTgg                                                           <  1:9075159/151‑1 (MQ=255)

                                                                                           ctcGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAAc                                                        <  1:11168509/151‑1 (MQ=255)

                                                                                           ctcGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAAc                                                        <  2:4003797/151‑1 (MQ=255)

                                                                                            tcGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGATGGTCTGCAAGCGCTGGAACg                                                       <  1:9625267/151‑1 (MQ=255)

                                                                                            tcGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACg                                                       >  1:6353710/1‑151 (MQ=255)

                                                                                             cggcggCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGGCGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGTACgg                                                      >  1:1552700/1‑151 (MQ=255)

                                                                                             cggcggCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACgg                                                      >  1:5502671/1‑151 (MQ=255)

                                                                                               gcggcCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCt                                                    <  2:10680469/151‑1 (MQ=255)

                                                                                                cggcCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCtt                                                   >  2:6428627/1‑151 (MQ=255)

                                                                                                 ggcCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTc                                                  <  1:5337795/151‑1 (MQ=255)

                                                                                                    caccaGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGc                                               >  2:5672005/1‑151 (MQ=255)

                                                                                                    caccaGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGc                                               >  1:5122563/1‑151 (MQ=255)

                                                                                                     accaGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCg                                              >  2:1765909/1‑151 (MQ=255)

                                                                                                     accaGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCg                                              >  1:2936679/1‑151 (MQ=255)

                                                                                                         gggTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGcc                                          >  1:5878749/1‑151 (MQ=255)

                                                                                                            tGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGGTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCt                                       <  2:229038/151‑1 (MQ=255)

                                                                                                              gAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCTGa                                     >  1:7220926/1‑151 (MQ=255)

                                                                                                              gAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCTGa                                     >  2:7034044/1‑151 (MQ=255)

                                                                                                              gAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGACGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCTGa                                     >  2:7848270/1‑151 (MQ=255)

                                                                                                               ccttGAAGAAGCCCCAGGACCAGAACCACTCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCTGAc                                    <  2:9448481/149‑1 (MQ=255)

                                                                                                                aTTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCTGAcc                                   <  1:11405660/151‑1 (MQ=255)

                                                                                                                   gaagaaGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGACCGGCTTCGGCGTGCCGCTGACcagc                                >  2:7248538/1‑151 (MQ=255)

                                                                                                                    aagaagCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCTGACcagca                               >  1:8618587/1‑151 (MQ=255)

                                                                                                                     agaagCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCTGACcagcag                              <  2:465669/151‑1 (MQ=255)

                                                                                                                     agaagCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCTGACcagcag                              <  2:6191264/151‑1 (MQ=255)

                                                                                                                        agCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCTGACCAGCAGGCg                           <  1:9954526/151‑1 (MQ=255)

                                                                                                                         gCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCTGACCAGCAGGCGa                          >  2:11094132/1‑151 (MQ=255)

                                                                                                                           cccAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCTGACCAGCAGGCGAcc                        <  2:251132/151‑1 (MQ=255)

                                                                                                                           cccAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCTGACCAGCAGGCGAcc                        >  2:2015428/1‑151 (MQ=255)

                                                                                                                            ccAGGACCAGAACGACTCCGGACTCTGCGGTTGCAGCCGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCTGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTTCCGCTGACCAGCAGTCGACCt                       <  2:5994004/151‑1 (MQ=255)

                                                                                                                            ccAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCTGACCAGCAGGCGACCt                       >  1:7971125/1‑151 (MQ=255)

                                                                                                                              aGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCTGACCAGCAGGCGACCTTc                     <  1:3431810/151‑1 (MQ=255)

                                                                                                                                 aCCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCTGACCAGCAGGCGACCTTCGTa                  <  1:2693878/151‑1 (MQ=255)

                                                                                                                                  ccAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCTGACCAGCAGGCGACCTTCGTAg                 <  1:4877236/151‑1 (MQ=255)

                                                                                                                                     gAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCTGACCAGCAGGCGACCTTCGTAGccc              <  1:7337335/151‑1 (MQ=255)

                                                                                                                                          aaTCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCTGACCAGCAGGCGACCTTCGTAGCCCGGAAt         >  1:3768629/1‑151 (MQ=255)

                                                                                                                                          aaTCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCTGACCAGCAGGCGACCTTCGTAGCCCGGAAt         <  2:9557353/151‑1 (MQ=255)

                                                                                                                                            tCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCTGACCAGCAGGCGACCTTCGTAGCCCGGAATcc       >  1:5087187/1‑151 (MQ=255)

                                                                                                                                             ccGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCTGACCAGCAGGCGACCTTCGTAGCCCGGAATCCg      >  1:2203207/1‑151 (MQ=255)

                                                                                                                                               ggACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCTGACCAGCAGGCGACCTTCGTAGCCCGGAATCCGGt    <  2:2942649/151‑1 (MQ=255)

                                                                                                                                                gACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCTGACCAGCAGGCGACCTTCGTAGCCCGGAATCCGGtt   <  1:4737374/151‑1 (MQ=255)

                                                                                                                                                gACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCTGACCAGCAGGCGACCTTCGTAGCCCGGAATCCGGtt   <  1:736602/151‑1 (MQ=255)

                                                                                                                                                gACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGGCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCTGACCCGCAGGCGACCTTCGTAGCCCGGAATCCGGtt   >  2:9160648/1‑151 (MQ=255)

                                                                                                                                                 aCTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCTGACCAGCAGGCGACCTTCGTAGCCCGGAATCCGGTTg  <  2:8870769/151‑1 (MQ=255)

CGCGCAGGCGTTTGTCGAAGGCGGACTTCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTTTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCTGACCAGCAGGCGACCTTCGTAGCCCGGAATCCGGTTG  >  NC_008463/2149277‑2149572

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`