BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_008463	3,673,534	3 bp→CTC	intergenic (+83/+13)	fabI → / ← PA14_RS16690	enoyl‑ACP reductase FabI/SurA N‑terminal domain‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_008463	3,673,534	0	T	C	99.8%	1746.8 / NA	447	intergenic (+83/+15)	fabI/PA14_RS16690	enoyl‑ACP reductase FabI/SurA N‑terminal domain‑containing protein
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (245/201); minor base G (1/0); total (246/201)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*	NC_008463	3,673,535	0	C	T	96.3%	1887.8 / 31.6	464	intergenic (+84/+14)	fabI/PA14_RS16690	enoyl‑ACP reductase FabI/SurA N‑terminal domain‑containing protein
Reads supporting (aligned to +/- strand): ref base C (10/7); new base T (246/201); total (256/208)
Fisher's exact test for biased strand distribution p-value = 8.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.44e-01
*	NC_008463	3,673,536	0	T	C	96.3%	1716.5 / 42.2	464	intergenic (+85/+13)	fabI/PA14_RS16690	enoyl‑ACP reductase FabI/SurA N‑terminal domain‑containing protein
Reads supporting (aligned to +/- strand): ref base T (10/7); new base C (246/201); total (256/208)
Fisher's exact test for biased strand distribution p-value = 8.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 464 total aligned reads displayed.

ATCCTCTACGTCGATGGCGGCTTCAACACTACCGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGNGTAAACGGCCTTTTTCT‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCTCGTCGTCGGAGATAGCCTCCTCCGGCTC  >  NC_008463/3673392‑3673680

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aTCCTCTACGTCGATGGCGGCTTCAACACTACCGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCAtc                                                                                                                                            >  1:10746829/1‑151 (MQ=255)

 tCCTCTACGTCGATGGCGGATTCAACCCTACCGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATTAAAAATGGCCGCTTCGTGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCAtct                                                                                                                                           <  1:10626143/151‑1 (MQ=255)

    tctACGTCGATGGCGGCTTCAACACTACCGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCgg                                                                                                                                        >  1:11207996/1‑151 (MQ=255)

     ctACGTCGATGGCGGCTTCAACACTACCGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGt                                                                                                                                       >  2:4792135/1‑151 (MQ=255)

     ctACGTCGATGGCGGCTTCAACACTACCGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGt                                                                                                                                       >  1:2518606/1‑151 (MQ=255)

      tACGTCGATGGCGGCTTCAACCCTACCGCCATGGGCCCGCGGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGtc                                                                                                                                      <  2:2765619/151‑1 (MQ=255)

         gTCGATGGCGGCTACAACACTACCGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCATTTTTCTTGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCa                                                                                                                                   <  1:5525592/151‑1 (MQ=255)

          tCGATGGCGGCTTCAACACTACCGCCATGGGCCCGCGGGACGACGACTGATCACCCCGCTCATGAAAAAGGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAg                                                                                                                                  <  2:4130551/151‑1 (MQ=255)

              tGGCGTCTACAACACACCCGCCATGGGCCGGGTGGCCGACGACTGACCACCCCGCTCATGAAAATGGGCCGATTCTAGCGGCCTTTTTAATGGGCGTAATAAACAAACGGCCGCGTACGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTAtt                                                                                                                              <  1:7764003/151‑1 (MQ=255)

                   gcTACAACACTACCGCCATGGGCCCGCGGGACGACGTCTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCg                                                                                                                         <  2:939149/151‑1 (MQ=255)

                     ttCAACACTACCGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGAc                                                                                                                       >  2:10770407/1‑151 (MQ=255)

                       cAACACTACCGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACtt                                                                                                                     >  1:6827318/1‑151 (MQ=255)

                       cAACACTACCGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACtt                                                                                                                     >  2:3278905/1‑151 (MQ=255)

                        aaCACTACCGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTc                                                                                                                    >  1:10529845/1‑151 (MQ=255)

                         acacTACCGCCATGGGCCCGCTGTACGACGAATGATCACCCCCCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGCCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTcc                                                                                                                   <  1:6183044/151‑1 (MQ=255)

                           acTACCGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGc                                                                                                                 <  1:8475257/151‑1 (MQ=255)

                            ctcccGCCTTGGGCCCGCTGGACGACGACTGATCACCCCGCTCATTAAAATTGGCCGCTTCGAGGGGCCTTTTTCTTGGGCGTAATAAACAAACGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGcc                                                                                                                <  1:239963/148‑1 (MQ=255)

                             tACCGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCTGCCTTTTTCATGGTCGGAATAAAGAAAAGGCCGCGTAAGCGTCCTTTTCTC‑TGCATCTCTGTCTCAGTATTTCTCTACTTCCGCCt                                                                                                               >  2:7710199/1‑151 (MQ=255)

                               ccGCCATGGGCCCGCTGGCCGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGTAATAAAGAAACGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTg                                                                                                             <  1:8756955/151‑1 (MQ=255)

                                 gCCATGGGCCCGCTGGCCGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGCGCGGCCTTTTTCATGGGCGTAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTc                                                                                                           <  1:7942843/151‑1 (MQ=255)

                                 gCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAACGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTc                                                                                                           <  2:5456502/151‑1 (MQ=255)

                                   cttGGGCCCGCTGGACGACTACTGATCACCCCGCACATTAAAAATGGCCGCTTCTAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGCCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCt                                                                                                         <  1:3873301/149‑1 (MQ=255)

                                   cATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCt                                                                                                         >  2:5700522/1‑151 (MQ=255)

                                    aTGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTg                                                                                                        >  2:6603664/1‑151 (MQ=255)

                                      gggCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCa                                                                                                      <  1:11059938/151‑1 (MQ=255)

                                      gggCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCa                                                                                                      >  1:7125315/1‑151 (MQ=255)

                                           cGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGa                                                                                                 <  2:11484119/151‑1 (MQ=255)

                                            gcgggACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGAc                                                                                                <  2:604524/148‑1 (MQ=255)

                                               ggACGACGACTGATAACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGAcggc                                                                                             >  2:2606969/1‑151 (MQ=255)

                                                    acgacTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAg                                                                                        >  1:4701637/1‑151 (MQ=255)

                                                     cgacTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAgg                                                                                       <  1:10084612/151‑1 (MQ=255)

                                                      gacTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAggc                                                                                      >  1:9456697/1‑151 (MQ=255)

                                                       acTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTACGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAggcg                                                                                     >  2:3055844/1‑151 (MQ=255)

                                                        cTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTGTTTCATGGGCGGACTCAAGAACAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGGATGTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAggcgg                                                                                    >  2:10987029/1‑151 (MQ=255)

                                                          ggtCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGgcggcg                                                                                  >  1:605792/3‑151 (MQ=255)

                                                          gATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGgcggcg                                                                                  <  2:8882496/151‑1 (MQ=255)

                                                              aCCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGt                                                                              >  1:2985025/1‑151 (MQ=255)

                                                              aCCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGt                                                                              >  2:4245315/1‑151 (MQ=255)

                                                                cccGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGACGGCGGCGCAGTCt                                                                            >  1:7478856/1‑151 (MQ=255)

                                                                   gCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTGTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGcc                                                                         >  1:7545247/1‑151 (MQ=255)

                                                                      cATGACAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGACTAAAGAAAAGGCCGCGTACGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGt                                                                      <  1:2217650/151‑1 (MQ=255)

                                                                       aTGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTc                                                                     <  2:3486021/151‑1 (MQ=255)

                                                                        agAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTcc                                                                    <  1:4462392/150‑1 (MQ=255)

                                                                           aaaaTGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCt                                                                 <  1:8179284/151‑1 (MQ=255)

                                                                             aaTGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTgc                                                               <  1:9297485/151‑1 (MQ=255)

                                                                              ttGGCCGCTTCGAGCGGCCTTTTTCATGGGGGGAATAAAGAAAAGGCCGCGTAAGGGGCCTTTTCTC‑TGCAGCTCGGTATCAGGATTTCTCGACTTCCGCCTGGTCGTGCAACTGACGGCGGAGGGGGGCGTAGTCTGGCTGTGAGCTgcg                                                              >  2:9930913/2‑151 (MQ=255)

                                                                                   cGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAg                                                         >  2:9605166/1‑151 (MQ=255)

                                                                                    gCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGc                                                        >  1:8956791/1‑151 (MQ=255)

                                                                                       tagAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACGTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAg                                                     <  2:2034717/149‑1 (MQ=255)

                                                                                       tCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAg                                                     >  1:5132832/1‑151 (MQ=255)

                                                                                       tCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAg                                                     <  1:6903250/151‑1 (MQ=255)

                                                                                          aGCGGCCTTTTTCATGGGCGGAATAACGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGaa                                                  <  1:3093935/151‑1 (MQ=255)

                                                                                            cGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGACgc                                                >  1:11532378/1‑151 (MQ=255)

                                                                                            cGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAgc                                                <  1:11597226/151‑1 (MQ=255)

                                                                                            cGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAgc                                                <  2:1253832/151‑1 (MQ=255)

                                                                                               ccTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGACgcggc                                             >  2:9160001/1‑151 (MQ=255)

                                                                                                  ttttCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCACCTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGt                                          >  2:1746430/1‑151 (MQ=255)

                                                                                                  ttttCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGt                                          >  1:4003540/1‑151 (MQ=255)

                                                                                                    ttCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTAc                                        >  2:815084/1‑151 (MQ=255)

                                                                                                       aTGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATg                                     >  2:2387490/1‑151 (MQ=255)

                                                                                                            cGGAATAAAGAAACGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCtt                                <  2:5038143/151‑1 (MQ=255)

                                                                                                            cGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCtt                                <  2:11372816/151‑1 (MQ=255)

                                                                                                               aaTCAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCCTCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTtctc                             <  1:6530438/151‑1 (MQ=255)

                                                                                                               aaTAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTCTTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGACGCGGCGGTACATGGCCTtctc                             >  2:6540000/1‑151 (MQ=255)

                                                                                                               aaTAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTtctc                             <  1:3495079/151‑1 (MQ=255)

                                                                                                                aTAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCtcg                            >  1:7804047/1‑151 (MQ=255)

                                                                                                                 tAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCTGTCTCAGTATTTCTCGACTTCCGCCTGGTCCTGCAACTGACGGCGGAAGGCGGCGACGTCTGCATGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCtcgt                           >  2:5901902/1‑151 (MQ=255)

                                                                                                                 tAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCtcgt                           >  1:8089505/1‑151 (MQ=255)

                                                                                                                  aaaGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCtcgtc                          <  2:3810646/151‑1 (MQ=255)

                                                                                                                  aaaGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCtcgtc                          >  2:6135705/1‑151 (MQ=255)

                                                                                                                  aaaGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGACTCTGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGATGCGGCGGTACATGGCCTTCtcgtc                          >  2:10593241/1‑151 (MQ=255)

                                                                                                                   aaGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCtcgtcg                         <  2:3650574/151‑1 (MQ=255)

                                                                                                                    aGACAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCtcgtcgt                        <  2:7430966/151‑1 (MQ=255)

                                                                                                                    aGAAAAGGCCGCGTAAGCGGCCTTGTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCtcgtcgg                        >  2:4523411/1‑150 (MQ=255)

                                                                                                                       aaaGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCTCGTCGTCgg                     <  1:9862912/151‑1 (MQ=255)

                                                                                                                        aaGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCTCGTCGTCGga                    >  2:8776216/1‑151 (MQ=255)

                                                                                                                         aGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCTCGTCGTCGgag                   >  2:7222261/1‑151 (MQ=255)

                                                                                                                          ggCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAACCAGGAAGCGGCGGTACATGGCCTTCTCGTCGTCGgaga                  >  2:6851900/1‑151 (MQ=255)

                                                                                                                           gCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCTCGTCGTCGGAGAt                 >  1:5904524/1‑151 (MQ=255)

                                                                                                                            ccGCGTACGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCTCGTCGTCGGAGATa                <  1:4348929/151‑1 (MQ=255)

                                                                                                                            ccGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCTCGTCGTCGGAGATa                <  2:2144477/151‑1 (MQ=255)

                                                                                                                             cgcgTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCTCGTCGTCGGAGATAg               <  1:7670036/151‑1 (MQ=255)

                                                                                                                             cgcgTAAGCGGCCTTTTCTC‑TGCATCGCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCTCGTCGTCGGAGATAg               <  2:5181911/151‑1 (MQ=255)

                                                                                                                                gTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCTCGTCGTCGGAGATAGcct            >  1:3581254/1‑151 (MQ=255)

                                                                                                                                gTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCTCGTCGTCGGAGATAGcct            >  1:5758353/1‑151 (MQ=255)

                                                                                                                                 tAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCTCGTCGTCGGAGATAGcctc           <  2:7821956/151‑1 (MQ=255)

                                                                                                                                     gcctccttttCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCTCGTCGTCGGAGATAGCctcctc        <  1:11416325/145‑1 (MQ=255)

                                                                                                                                     cGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCTCGTCGTCGGAGATAGCCtcctcc       <  2:6961969/151‑1 (MQ=255)

                                                                                                                                     cGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCTCGTCGTCGGAGATAGCCtcctcc       >  1:3252102/1‑151 (MQ=255)

                                                                                                                                     cGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCTCGTCGTCGGAGATAGCCtcctcc       <  2:8356482/151‑1 (MQ=255)

                                                                                                                                      ggCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCTCGTCGTCGGAGATAGCCTCCTCCg      >  1:4907994/1‑151 (MQ=255)

                                                                                                                                      ggCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCTCGTCGTCGGAGATAGCCTCCTCCg      >  2:10184422/1‑151 (MQ=255)

                                                                                                                                       gCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCTCGTCGTCGGAGATAGCCTCCTCCgg     >  2:10392271/1‑151 (MQ=255)

                                                                                                                                       gCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCTCGTCGTCGGAGATAGCCTCCTCCgg     >  1:9626021/1‑151 (MQ=255)

                                                                                                                                          cttttCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCTCGTCGTCGGAGATAGCCTCCTCCGGCt   <  2:8699639/150‑1 (MQ=255)

                                                                                                                                           ttttCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCTCGTCGTCGGAGATAGCCTCCTCCGGCTc  >  1:1696366/1‑151 (MQ=255)

                                                                                                                                           ttttCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCTCGTCGTCGGAGATAGCCTCCTCCGGCTc  >  1:2013834/1‑151 (MQ=255)

                                                                                                                                           ttttCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCTCGTCGTCGGAGATAGCCTCCTCCGGCTc  >  1:6653924/1‑151 (MQ=255)

                                                                                                                                           ttttCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCTCGTCGTCGGAGATAGCCTCCTCCGGCTc  >  1:7235731/1‑151 (MQ=255)

                                                                                                                                           ttttCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCTCGTCGTCGGAGATAGCCTCCTCCGGCTc  >  1:10257995/1‑151 (MQ=255)

|||

ATCCTCTACGTCGATGGCGGCTTCAACACTACCGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGNGTAAACGGCCTTTTTCT‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCTCGTCGTCGGAGATAGCCTCCTCCGGCTC  >  NC_008463/3673392‑3673680

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`