Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084635,125,7990TG24.4% 311.4 / 31.8 229intergenic (‑54/+32)PA14_RS23455/PA14_RS23460glutathione S‑transferase N‑terminal domain‑containing protein/cytochrome c1
Reads supporting (aligned to +/- strand):  ref base T (127/46);  new base G (51/5);  total (178/51)
Fisher's exact test for biased strand distribution p-value = 5.26e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 232 total aligned reads displayed.

TACCGCCACGTTCTTCTCGGCCAGGACCAGGCGGATGCGATGGCAGTAATGATCGGCAGGGTCGGAATAGCAGGTCAGCTTGTTGATTGAAGCCATGGAGCCCTCCTCGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGTAGGGCGCGCGCTTCGAAGACCAACGTGCGGGTTAGTGCACGTCCTTCCAGTACTCGCGCTTGAGGAGATAGGCGAATACGAAGAAGAAGGCCAGGTAGAGCAGGACGTAGGTACCGATACGCTCGGAGGCCAGCTTGTTCGGGTTGGCC  >  NC_008463/5125650‑5125948
                                                                                                                                                     |                                                                                                                                                     
tACCGCCACGTTCTTCTCGGCCAGGACCAGGCGGATGCGATGGCAGTAATGATCGGCAGGGTCGGAATAGCAGGTCAGCTTGTTGATTGAAGCCATGGAGCCCTCCTCGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGt                                                                                                                                                       >  2:1262865/1‑150 (MQ=255)
 aCCGCCACGTTCTTCTCGGCCAGGACCAGGCGGATGCGATGGCAGTAATGATCGGCAGGGTCGGAATAGCAGGTCAGCTTGTTGATTGAAGCCATGGAGCCCTCCTCGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGTa                                                                                                                                                      >  1:10827319/1‑150 (MQ=255)
 aCCGCCACGTTCTTCTCGGCCAGGACCAGGCGGATGCGATGGCAGTAATGATCGGCAGGGTCGGAATAGCAGGTCAGCTTGTTGATTGAAGCCATGGAGCCCTCCTCGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGTa                                                                                                                                                      <  1:2701411/150‑1 (MQ=255)
  ccGCCACGTTCTTCTCGGCCAGGACCAGGCGGATGCGATGGCAGTAATGATCGGCAGGGTCGGAATAGCAGGTCAGCTTGTTGATTGAAGCCATGGAGCCCTCCTCGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGTAg                                                                                                                                                     >  1:4250563/1‑150 (MQ=255)
   cGCCACGTTCTTCTCGGCCAGGACCAGGCGGATGCGATGGCAGTAATGATCGGCAGGGTCGGAATAGCAGGTCAGCTTGTTGATTGAAGCCATGGAGCCCTCCTCGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGTAgg                                                                                                                                                    >  1:6701832/1‑150 (MQ=255)
    gCCACGTTCTTCTCGGCCAGGACCAGGCGGATGCGATGGCAGTAATGATCGGCAGGGTCGGAATAGCAGGTCAGCTTGTTGATTGAAGCCATGGAGCCCTCCTCGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGGAggg                                                                                                                                                   >  2:7527010/1‑150 (MQ=255)
       aCGTTCTTCTCGGCCAGGACCAGGCGGATGCGATGGCAGTAATGATCGGCAGGGTCGGAATAGCAGGTCAGCTTGTTGATTGAAGCCATGGAGCCCTCCTCGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGTAGGgcgc                                                                                                                                                >  2:9710579/1‑150 (MQ=255)
        cGTTCTTCTCGGCCAGGACCAGGCGGATGCGATGGCAGTAATGATCGGCAGGGTCGGAATAGCAGGTCAGCTTGTTGATTGAAGCCATGGAGCCCTCCTCGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGTAGGgcgcg                                                                                                                                               >  2:7496272/1‑150 (MQ=255)
         gTTCTTCTCGGCCAGGACCAGGCGGATGCGATGGCAGTAATGATCGGCAGGGTCGGAATAGCAGGTCAGCTTGTTGATTGAAGCCATGGAGCCCTCCTCGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGTAGGgcgcgc                                                                                                                                              <  2:2234630/150‑1 (MQ=255)
         gTTCTTCTCGGCCAGGACCAGGCGGATGCGATGGCAGTAATGATCGGCAGGGTCGGAATAGCAGGTCAGCTTGTTGATTGAAGCCATGGAGCCCTCCTCGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGTAGGgcgcgc                                                                                                                                              >  1:2495174/1‑150 (MQ=255)
          ttcttcTCGGCCAGGACCAGGCGGATGCGATGGCAGTAATGATCGGCAGGGTCGGAATAGCAGGTCAGCTTGTTGATTGAAGCCCTGGAGCCCTCCTCGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGGAGGgcgcgcg                                                                                                                                             >  1:4189102/1‑150 (MQ=255)
          ttcttcTCGGCCAGGACCAGGCGGATGCGATGGCAGTAATGATCGGCAGGGTCGGAATAGCAGGTCAGCTTGTTGATTGAAGCCATGGAGCCCTCCTCGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGTAGGgcgcgcg                                                                                                                                             <  1:6855654/150‑1 (MQ=255)
                 cGGCCAGGACCAGGCGGATGCGATGGCAGTAATGATCGGCAGGGTCGGAATAGCAGGTCAGCTTGTTGATTGAAGCCATGGAGCCCTCCTCGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGTAGGGCGCGCGCTTCGaa                                                                                                                                      >  2:4221961/1‑150 (MQ=255)
                  ggCCAGGACCAGGCGGATGCGATGGCAGTAATGATCGGCAGGGTCGGAATAGCAGGTCAGCTTGTTGATTGAAGCCATGGAGCCCTCCTCGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGTTGGGCGCGCGCTTCGaat                                                                                                                                     >  1:3325056/1‑149 (MQ=255)
                   gCCAGGACCAGGCGGATGCGATGGCAGTAATGATCGGCAGGGTCGGAATAGCAGGTCAGCTTGTTGATTGAAGCCATGGAGCCCTCCTCGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGGAGGGCGCGCGCGTCGAAGa                                                                                                                                    >  1:8397411/1‑150 (MQ=255)
                     cAGGACCAGGCGGATGCGATGGCAGTAATGATCGGCAGGGTCGGAATAGCAGGTCAGCTTGTTGATTGAAGCCATGGAGCCCTCCTCGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGGAGGGCGCGCGCGTCGAAGAcc                                                                                                                                  >  1:1152886/1‑150 (MQ=255)
                       ggACCAGGCGGATGCGATGGCAGTAATGATCGGCAGGGTCGGAATAGCAGGTCAGCTTGTTGATTGAAGCCATGGAGCCCTCCTCGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGTAGGGCGCGCGCGTCGAAGACCaa                                                                                                                                >  2:4889810/1‑150 (MQ=255)
                       ggACCAGGCGGATGCGATGGCAGTAATGATCGGCAGGGTCGGAATAGCAGGTCAGCTTGTTGATTGAAGCCATGGAGCCCTCCTCGCAGGTCGACGTGTGGGAGGGGAAAAGACGCGCGCGCCCCGTAGGGCGCGCGCTTCGAAGAACaa                                                                                                                                >  2:846520/1‑150 (MQ=255)
                          ccAGGCGGATGCGATGGCAGTAATGATCGGCAGGGTCGGAATAGCAGGTCAGCTTGTTGATTGAAGCCATGGAGCCCTCCTCGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGTAGGGCGCGCGCTTCGAAGACCAACGt                                                                                                                             >  2:4468791/1‑150 (MQ=255)
                          ccAGGCGGATGCGATGGCAGTAATGATCGGCAGGGTCGGAATAGCAGGTCAGCTTGTTGATTGAAGCCATGGAGCCCTCCTCGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGTAGGGCGCGCGCTTCGAAGACCAACGt                                                                                                                             >  1:8825674/1‑150 (MQ=255)
                             ggCGGATGCGATGGCAGTAATGATCGGCAGGGTCGGACTAGCAGGTCAGCTTGTTGATTGAAGCCATGGAGCCCTCCTCGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGGAGGGCGCGCGCTTCGTAGACCCACGTGCg                                                                                                                          >  2:3740433/1‑150 (MQ=255)
                                ggATGCGATGGCAGTAATGATCGGCAGGGTCGGAATAGCAGGTCAGCTTGTTGATTGAAGCCATGGAGCCCTCCTCGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGTCGGGCGCGCGCTTCGAAGACCAACGTGCGGGt                                                                                                                       >  2:2481721/1‑150 (MQ=255)
                                 gATGCGATGGCAGTAATGATCGGCAGGGTCGGAATAGCAGGTCAGCTTGTTGATTGAAGCCATGGAGCCCTCCTCGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGTAGGGCGCGCGCTTCGAAGACCAACGTGCggggt                                                                                                                      >  1:9605783/1‑148 (MQ=255)
                                   tGCGATGGCAGTAATGATCGGCAGGGTCGGAATAGCAGGTCAGCTTGTTGATTGAAGCCATGGAGCCCTCCTCGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGTAGGGCGCGCGCTTCGAAGACCAACGTGCGGGTTAg                                                                                                                    >  1:6195871/1‑150 (MQ=255)
                                   tGCGATGGCAGTAATGATCGGCAGGGTCGGAATAGCAGGTCAGCTTGTTGATTGAAGCCATGGAGCCCTCCTCGCAGGTCGACTTGTGGGAGGGGAAAAGACGCGCGCGCCCCGTTGGGCGCGCGCTTCGAAGACCCACGTGCGGGTTAg                                                                                                                    >  1:7386404/1‑150 (MQ=255)
                                   tGCGAGGGCAGTAATGATCGGCAGGGTCGGAATAGCAGGTCAGCTTGTTGATTGAAGCCATGGAGCCCTCCTCGCAGGTCGACGTGTGGGAGGTGAAAAGACGCGCGCGCCCCGTGGGGCGCGCGCTTCGAAGACCAACGTGCGGGTGAg                                                                                                                    >  2:6811690/1‑150 (MQ=255)
                                    gCGATCGCAGTAATGATCGGCAGGGTCGGAATAGCAGGTCAGCTTGTTGATTGAAGCCATGGAGCCCTCCTCGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGGAGGGCGCGCGCTTGGAAGTCCAAcgggcggggttgt                                                                                                                   >  1:2009182/1‑145 (MQ=255)
                                     cGATGGCAGTAATGATCGGCAGGGTCGGAATAGCAGGTCAGCTTGTTGATTGAAGCCATGGAGCCCTCCTCGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGTAGGGCGCGCGCTTCGAAGACCAACGTGCGGGTTAGTg                                                                                                                  >  2:1646400/1‑150 (MQ=255)
                                      gATGGCAGTAATGATCGGCAGGGTCGGAATAGCAGGTCAGCTTGTTGATTGAAGCCATGGAGCCCTCCTCGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGTAGGGCGCGCGCTTCGAAGACCAACGTGCGGGTTAGTGc                                                                                                                 >  2:4372706/1‑150 (MQ=255)
                                          gCAGTAATGATCGGCAGGGTCGGAATAGCAGGTCAGCTTGTTGATTGAAGCCATGGAGCCCTCCTCGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGTAGGGCGCGCGCTTCGAAGACCAACGTGCGGGTTAGTGCACGt                                                                                                             >  2:9300889/1‑150 (MQ=255)
                                           cAGTAATGATCGGCAGGGTCGGAATAGCAGGTCAGCTTGTTGATTGAAGCCATGGAGCCCTCCTCGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGTAGGGCGCGCGCTTCGAAGACCAACGTGCGGGTTAGTGCACGTc                                                                                                            >  1:7872187/1‑150 (MQ=255)
                                           cAGTAATGATCGGCAGGGTCGGAATAGCAGGTCAGCTTGTTGATTGAAGCCATGGAGCCCTCCTCGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGGAGGGCGCGCGCTTCGAAGACCAACGTGCGGGTTAGTGCACGTc                                                                                                            >  2:3411483/1‑150 (MQ=255)
                                           cAGTAATGATCGGCAGGGTCGGAATAGCAGGTCAGCTTGTTGATTGAAGCCATGGAGCCCTCCTCGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCCCCCCGTAGGGCGCGCGCGTCGAAGACCAACGTGCGGGTTAGTGCACGTc                                                                                                            >  1:9184418/1‑150 (MQ=255)
                                             gTAATGATCGGCAGGGTCGGAATAGCAGGTCAGCTTGTTGATTGAAGCCATGGAGCCCTCCTCGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGTAGGGCGCGCGCTTCGAAGACCAACGTGAGGGTTAGTGCACGTCCt                                                                                                          >  2:1216826/1‑150 (MQ=255)
                                               aaTGATCGGCAGGGTCGGAATAGCAGGTCAGCTTGTTGATTGAAGCCATGGAGCCCTCCTCGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGTAGGGCGCGCGCTTCGAAGACCAACGTGCGGGTTAGTGCACGTCCTTc                                                                                                        >  2:7123610/1‑150 (MQ=255)
                                                aTGATCGGCAGGGTCGGAATAGCAGGTCAGCTTGTTGATTGAAGCCATGGAGCCCTCCTCGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGTAGGGCGCGCGCTTCGAAGACCAACGTGCGGGTTAGTGCACGTCCTTcc                                                                                                       >  2:6126362/1‑150 (MQ=255)
                                                  gATCGGCAGGGTCGGAATAGCAGGTCAGCTTGTTGATTGAAGCCATGGAGCCCTCCTCGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGTAGGGCGCGCGCTTCGAAGACCCACGTGCGGGGTAGTGCACGTCCTTCCAg                                                                                                     >  2:10070697/1‑150 (MQ=255)
                                                  gATCGGCAGGGTCGGAATAGCAGGTCAGCTTGTTGATTGAAGCCATGGAGCCCTCCTCGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGTAGGGCGCGCGCTTCGAAGACCAAAGTGCGGGTTATTGCACGACCTTACAg                                                                                                     >  2:2516337/1‑150 (MQ=255)
                                                  gATCGGCAGGGTCGGAATAGCAGGTCAGCTTGTTGATTGAAGCCATGGAGCCCTCCTCGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCCTGGGGCGCGCGCGTAGAAGACCAACGTGGGGGGTAGTGGACGTACTTACAg                                                                                                     >  1:3749546/1‑150 (MQ=255)
                                                   aTCGGCAGGGTCGGAATAGCAGGTCAGCTTGTTGATTGAAGCCATGGAGCCCTCCTCGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGGAGGGGGCGCGCGTCGAAGACCAACGTGCGGGTTAGTGCACGTACTTCCAGt                                                                                                    >  1:5922635/1‑150 (MQ=255)
                                                    tCGGCCGGGTCGTAATAGCTGGACTGTTTGTTTTTTTACCCCTTGGCGCCCTCCTCGCTGTGACCCTTGTGGTTGGTTAAAAGACGCGCGCCCCCTGGAGGGCGCCCGCTTCGAAGACCAACGTGCGGGTTAGTGCACGTCCTTCCAGTa                                                                                                   <  2:1426059/150‑1 (MQ=255)
                                                    tCGGCAGGGTCGGAATAGCAGGTCAGCTTGTTGATTGAAGCCATGGAGCCCTCCTCGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGTAGGGCGCGCGCTTCGAAGACCAACGTTCGGGTGAGTGCACGTCCTTCCAGTa                                                                                                   >  1:7040548/1‑150 (MQ=255)
                                                    tCGGCAGGGTCGGAATAGCAGGTCAGCTTGTTGATTGAAGCCATGGAGCCCTCCTCGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGGAGGGCGCGCGCGTCGAAGACCAACGGGCGGGGTCGTGCACGTCCTTcccgga                                                                                                   >  1:1419831/1‑146 (MQ=255)
                                                      ggCAGGGTCGGAATAGCAGGTCAGCTTGTTGATTGAAGCCATGGAGCCCTCCTCGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGGAGGGCGCGCGCTTCGAAGTACAACGTGCGGGTTAGTGCACGTCCTTCCAGTACt                                                                                                 >  1:8006717/1‑150 (MQ=255)
                                                       gCAGGGTCGGAATAGCAGGTCAGCTTGTTGATTGAAGCCATGGAGCCCTCCTCGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGGAGGGCGCGCGCTTCGAAGACCAACGTGCGGGTTAGTGCACGTCCTTCCAGTACTc                                                                                                >  2:5335685/1‑150 (MQ=255)
                                                          gggTCGGAATAGCAGGTCAGCTTGTTGATTGACGCCATGGAGCCCTCCTCGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGTAGGGCGCGCGCTTCGAAGACCAACGTGCGGGTTAGTGCACGTCCTTCCAGGACAcgcg                                                                                             >  2:195449/1‑150 (MQ=255)
                                                          gggTCGGAATAGCAGGTCAGCTTGTTGATTGAAGCCATGGAGCCCTCCTCGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGTAGGGCGCGCGCGTCGAAGACCAACGTGCGGGTTAGTGCACGTCCTTCCAGTACTcgcg                                                                                             >  1:3927928/1‑150 (MQ=255)
                                                           ggTCGTATTAGCTGGACTTCTTTTTGATTCACGCCAGGGCGCCCCCCTCGCTGGGCTCGTTGTGGGTGGTTAAATCACGCGCGCCCCCCGTCGGGCGCCCGCTTCGAAGACCAACGTGCGGGTTAGTGCACGTCCTTCCAGTACTCgcgc                                                                                            <  2:10783778/150‑1 (MQ=255)
                                                           ggTCGGAATAGCAGGTCAGCTTGTTGATTGAAGCCATGGAGCCCTCCTCGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGTAGGGCGCGCGCTTCGAAGACCAACGTGCGGGTTAGTGCACGTCCTTCCAGTACTCgcgc                                                                                            >  2:32077/1‑150 (MQ=255)
                                                           ggTCGGAATAGCAGGTCAGCTTGTTGATTGAAGCCATGGAGCCCTCCTCGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGGCGGGCGCGCGCGTGGAAGACCAACGTGCGGGGTCGTGCACGGACTTCCAGTGCTcgggg                                                                                            >  1:3821672/1‑147 (MQ=255)
                                                            gTCGGAATAGCAGGTCAGCTTGTTGATTGAAGCCATGGAGCCCTCCTCGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGGAGGGGGCGCGCGTCGAAGTCCAACGTGGGGGTTAGTGCACGTCCTTCCAGTAAGcgcggt                                                                                           >  1:10272952/1‑148 (MQ=255)
                                                                 aaTAGCAGGTCAGCTTGTTGATTGAAGCCATGGAGCCCTCCTCGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGGAGGGCGCGCGCTTCGAAGACCAACGTGCGGGTTAGTGAACGTCCTTCCAGTACTCGCGCGTgagg                                                                                      >  2:1521668/1‑150 (MQ=255)
                                                                   tAGCTGGTCAGATTTTTTATTTAACCCAGGTAGCCCCCCTCGCAGGTCTACTGGTGGGAGGTTAAACGACGCGCGCGCCCCGTTGGGCGCCCGCTTCGAAGACCAACGTGCGGGTTAGTGCACGTCCTTCCAGTACTCGCGCTTgaggag                                                                                    <  2:7026741/150‑1 (MQ=255)
                                                                      cAGGTCAGCTTGTTGATTGAAGCCATGGAGCCCTCCTCGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGTAGGGCGCGCGCTTCGAAGACCAACGTGCGGGTTAGTGCACGTCCTTCCAGTACTCGCGCTTGAGGAGATa                                                                                 >  2:10659715/1‑150 (MQ=255)
                                                                        ggTCAGCTTGTTGATTGAAGCCATGGAGCCCTCCTCGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGTAGGGCGCGCGCGTCGAAGACCAACGTGCGGGGTTGTGCACGTCCTTCCAGTACTCGCGCGTGTGGAGTTAgg                                                                               >  1:2587652/1‑150 (MQ=255)
                                                                        ggTCAGCTTGTTGATTGAAGCCATGGAGCCCTCCTCGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGGAGGGCGCGCGCTTCGAACACCAACGTGCGGGTTAGTGCACGTCCTTACAGTAATCGCGCTTGAGGAGATAgg                                                                               >  1:1793047/1‑150 (MQ=255)
                                                                         gTCAGCTTGTTGATTGAAGCCATGGAGCCCTCCTCGCAGGTCGTCTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGTGGGGGGGGCGGTGCGGAGACCAACGGGGGGGTTAGTGCACGTCCTGCCAGTAGTCGCGCTGGAGGAGTTTGGc                                                                              >  1:3463443/1‑150 (MQ=255)
                                                                             gcgtgttgATTGAAGCCATGGAGCCCTCCTCGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGTATGGCGCGCCCTTCGCAGAACAACGTGCGGGTGAGTGCCCCTGCTTACAATAAACGCGCTGGAGGGGATATGCGAAt                                                                          >  1:4768302/4‑150 (MQ=255)
                                                                               ttgttgATTGAAGCCATGGAGCCCTCCTCGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGTAGGGCGCGCGCTTCGAAGACCAACGTGCGGGTTAGTGCACGTCCTTCCAGTACTCGCGCTTGAGGAGATAGGCGAATAc                                                                        >  2:10019137/1‑150 (MQ=255)
                                                                               ttgttgATTGAAGCCATGGAGCCCTCCTCGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCCTAGGGCGCGCGCTTCGAATACCAAACAGCGGGTTAGTGCACGTACATACACTACTCGCGCGTGAGGAGATATGCGAATaa                                                                        >  1:9265715/1‑149 (MQ=255)
                                                                                 gttgATTGAAGCCATGGAGCCCTCCTCGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGTAGGGCGCGCGCGTCGAAGACCAACGTGCGGGGTAGTGCACGTCCATCCAGTACTCGCGCTTGAGGAGATAGGCGAATACga                                                                      >  1:10656739/1‑150 (MQ=255)
                                                                                  ttgattgaAGCCATGGAGCCCTCCTCGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGGAGGGCGCGCGCTTCGAAGACCAACGTGCGGGGTAGTGCACGTCCTTACAGTACACGCGCGTGAGGAGATAGGCGAATACgaa                                                                     >  1:9729043/1‑150 (MQ=255)
                                                                                   tgattgaAGCCATGGAGCCCTCCTCGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGGAGGGCGCGCGCTTCGAAGACCAACGTGCGGGTTAGTGCACGTCCTTCCAGTACTCGCGCTTGAGGAGATAGGCGAATACgaag                                                                    >  2:1325165/1‑150 (MQ=255)
                                                                                     attgaAGCCATGGAGCCCTCCTCGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGTAGGGCGCGCGCTTCGAAGACCAACGTGCGGGGTAGTGCACGTCCTTCCAGTACTCGCGCTTGTGGTGGTAGGCGGATACgaagaa                                                                  >  1:7141889/1‑150 (MQ=255)
                                                                                     attgaAGCCATGGAGCACTCCTCGCAGGTCGACTTGTGGGAGGTGAATAGACTCGCGCCCCACGTAGGGCGCGGGCGTCTAATACCCCCGTGACGGTTACTTCAAGACCATCCACTACTAGCGCGGGTTGAGATAGGCGaagaagaagga                                                                  >  2:4608097/1‑148 (MQ=255)
                                                                                      ttgaAGCCATGGCGCCCTCCTCGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCCGAGGGCGCGCGCGTAGAAGACCAACCTGCGGGTTAGTGCACGTCCTTCCACTAATCGCGCGTGAGGGGATAGGCGAATaagaagaag                                                                 >  1:1369292/1‑150 (MQ=255)
                                                                                      ttgaAGCCATGGAGCCCTCCTCGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGTAGGGCGCGCGCTTCGAAGACCAACGTGCGGGGTAGTGCACGTCCTTCCAGTACTCGCGCGTGAGGAGATAGGCGTATACgaagaag                                                                 >  2:2815327/1‑150 (MQ=255)
                                                                                         aaGCCATGGAGCCCTCCTCGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGTATGGCGCGCGCTTAGAAGAACAACCTGAGGGTTATTTCACGTCAATACAGTAATATCGCATTAGGATATAGGAGAATAATaagaagaag                                                              >  2:1015221/1‑150 (MQ=255)
                                                                                         aaGCCATGGAGCCCTCCTCGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGTAGGGCGCGCGCTTCGAAGACCAACGTGCGGGGTAGTGCACGTCCTTCCAGTACTCGCGCTTGAGGAGGTAGGCGAATACGaagaagaag                                                              >  2:1005451/1‑150 (MQ=255)
                                                                                         aaGCCATGGAGCCCTCCTCGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGTAGGGCGCGCGCGTCGAAGACCAACGTGCGGGTTAGTGCACGTCCTTCCAGTACTCGCGCTTGTGGAGATAGGCGAATACGtagaagaag                                                              >  1:8884158/1‑150 (MQ=255)
                                                                                              aTGGAGCCCTCCTCGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGTAGGGCGCGCGCTTCGAAGACCAACGTGCGGGTTAGTGCACGTCCTTCCAGTACTCGCGCTTGAGGAGATAGGCGAATACGAAGAAGAAGGCCAg                                                         >  1:9162891/1‑150 (MQ=255)
                                                                                                ggAGCCCTCCTCGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGTAGGGCGCGCGCGTCGAAGACCAACGTGCGGGTTCGTGCACGTCCTTCCAGTACTCGCGCGTGAGGAGGTAGGCGAATACGAAGAAGAAGGCCAGGt                                                       >  2:6362877/1‑150 (MQ=255)
                                                                                                 gAGCCCTCCGCGCTGGTTGAGTGGGGGTGGGTGAAATGACGCGCGCGCCCCGTGGGGCGCGCGCTTCGAAGACCAACGTGCGGGTTAGTGCACGTCCTTCCAGTACTCGCGCTTGAGGAGATAGGCGAATACGAAGAAGAAGGCCAGGTa                                                      <  2:8046924/150‑1 (MQ=255)
                                                                                                    ccccccTCGCCGGTCTAGTTTGGGGTGGTTAAACCCCGCGCGCCCCCCGTGGGGCGCGCGCTTCGAAGACCAACGTGCGGGTTAGTGCACGTCCTTCCAGTACTCGCGCTTGAGGAGATAGGCGAATACGAAGAAGAAGGCCAGGTagag                                                   <  2:5558312/150‑1 (MQ=255)
                                                                                                    cccTCCTCGAAGTTCGACTTGTGGTAGGTTAAACGACGCGCGCGCCCCGTAGGGCGCGCGCTTCGAAGACCAACGTGCGGGTTAGTGCACGTCCTTCCAGTACTCGCGCTTGAGGAGATAGGCGAATACGAAGAAGAAGGCCAGGTagag                                                   <  2:7948227/150‑1 (MQ=255)
                                                                                                         ctcGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGGAGGGCGCGCGCTTCGAAGACCAACGTGCGGGTTAGTGCACGTCCTTCCAGTACTCGCGCTTGAGGAGATAGGCGAATACGAAGAAGAAGGCCAGGTAGAGCAGGa                                              >  2:4488608/1‑150 (MQ=255)
                                                                                                          tcGCTGTTCGATTTGGGGGAGGTTAAAAGAGGCGCGCGCCCCGTCGGGCGCGCGCTTCGAAGACCAACGTGCGGGTTAGTGCACGTCCTTCCAGTACTCGCGCTTGAGGAGATAGGCGAATACGAAGAAGAAGGCCAGGTAGAGCAGGAc                                             <  2:5225594/150‑1 (MQ=255)
                                                                                                          tcGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGTAGGGCGCGCGCTTCGAAGACCAACGTGCGGGTTAGTGCACGTCCTTCCAGTACTCGCGCGTGAGGAGATAGGCGAATACGAAGAAGAAGGCCAGGTAGAGCAGGAc                                             >  2:7221294/1‑150 (MQ=255)
                                                                                                           cGCTGGTCTACTGGGGGGTGGTCAAACGACGGGCGCGCCCGTTAGGGCGCCCGCTTCGAAGACCAACGTGCGGGTTAGTGCACGTCCTTCCAGTACTCGCGCTTGAGGAGATAGGCGAATACGAAGAAGAAGGCCAGGTAGAGCAGGACg                                            <  2:11033258/150‑1 (MQ=255)
                                                                                                             cAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGGAGGGCGCGCGCTTCGAAGACCAACGTGCGGGTTAGTGCACGTCCATCCAGTACACTCGCTTGAGGAGATAGGCGAATACGACTAAGAAGGCCAGGTAGATCAGGACGTa                                          >  2:238882/1‑150 (MQ=255)
                                                                                                             cAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGGAGGGCGCGCGCTTCGAAGACCAACGTGCGGGGTAGTGCACGTCCGTCCACTACACCCGCGTGAGGAGATAAGCGCATAAGAAGAAGAATGCCAGGTAGAGCAGGACGTa                                          >  1:7535051/1‑150 (MQ=255)
                                                                                                             cAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCCTAGGGCGCGCGCATCGAAGACCAACGGGCGGGTTAGTTCACGTCCTTCCAGTACTCGCGCATGAGGAGATAGGCGAATACCAAGAAGAAGGCCACGTAGAGCAGGACGTa                                          >  1:4352462/1‑150 (MQ=255)
                                                                                                                  cgcgttGTGGGTGGTCAAAAGCCGCGGGCCCCCCGTTGGGCGCGCGCTTCGAAGACCAACGTGCGGGTTAGTGCACGTCCTTCCAGTACTCGCGCTTGAGGAGATAGGCGAATACGAAGAAGAAGGCCAGGTAGAGCAGGACGTAGGTAc                                     <  2:3088546/146‑1 (MQ=255)
                                                                                                                       tgtgGGAGGTGAAAAGACGCGCGCGCCCCGTAGGGCGCGCGCTTCGAAGACCAACGTGCGGGTTAGTGCACGTCCTTCCAGTACTCGCGCTTGTGGAGATAGGCGAATACGAAGAAGAAGGCCAGGTAGAGCAGGACGTAGGTACCGATa                                >  2:1783946/1‑150 (MQ=255)
                                                                                                                        gtgggtggTGAAAAGACGCGCGCCCCCCGTTGGGCGCGCGCTTCGAAGACCAACGTGCGGGTTAGTGCACGTCCTTCCAGTACTCGCGCTTGAGGAGATAGGCGAATACGAAGAAGAAGGCCAGGTAGAGCAGGACGTAGGTACCGATAc                               <  2:8754177/150‑1 (MQ=255)
                                                                                                                            gAGGTGAAAAGACGCGCGCCCCCCGTATGGCGCGCGCATCGAAGACCAACGTGCGGGTTAGTTCAAGTAAATCCAGTACTCGCGCTAGATGATATATGCGAATAAGAATAAGAAGGACATGTAGAGCAGGAAGTATGTAACGATaagata                           >  2:9784102/1‑145 (MQ=255)
                                                                                                                                   aacgACGCGCGCGCCCCGTCGGGCGCGCGCTTCGAAGACCAACGTGCGGGTTAGTGCACGTCCTTCCAGTACTCGCGCTTGAGGAGATAGGCGAATACGAAGAAGAAGGCCAGGTAGAGCAGGACGTAGGTACCGATACGCTCGGAGGcc                    <  2:7671153/147‑1 (MQ=255)
                                                                                                                                     aGACGCGCGCGCCCCCGAGGGCGCGCGCGTCGAAGACCAACCTGCGGGTTAGTGCACGTCCTTCCAGTACGAGCGCTTGAGGAGATAGGCGAATACGAAGAAGAAGGCCAGGGAGAGCAGGACGTAGGTACCGATACGCTCGGAGGCCAg                  >  2:6429431/1‑150 (MQ=255)
                                                                                                                                         gcgcgcgcCCCGTAGGGCGCGCGCTTCGAAGACCAACGTGCGGGTTAGTGCACGTCCTTCCAGTACTCGCGCTTGAGGAGATAGGCGAATACGAAGAAGAAGGCCAGGTAGTGCAGGACGTAGGTACCGATACGCTCGGAGGCCAGCTTg              >  1:5455789/1‑150 (MQ=255)
                                                                                                                                             gcgcCCCGTAGGGCGCGCGCTTCGAAGACCAACGTGCGGGTTAGTGCACGTCCTTCCAGTACTCGCGCTTGAGGAGATAGGCGAATACGAAGAAGAAGGCCAGGTAGAGCAGGACGTAGGTACCGATACGCTCGGAGGCCAGCTTGTTCg          >  1:3106741/1‑150 (MQ=255)
                                                                                                                                              cgcCCCGTAGGGCGCGCGCTTCGAAGACCAACGTGCGGGTTAGTGCACGTCCTTCCAGTACTCGCGCTTGAGGAGATAGGCGAATACGAAGAAGAAGGCCAGGTAGAGCAGGACGTAGGTACCGATACGCTCGGAGGCCAGCTTGTTCgg         >  1:6601137/1‑150 (MQ=255)
                                                                                                                                                ccccGTCGGGCGCGCGCTTCGAAGACCAACGTGCGGGTTAGTGCACGTCCTTCCAGTACTCGCGCTTGAGGAGATAGGCGAATACGAAGAAGAAGGCCAGGTAGAGCAGGACGTAGGTACCGATACGCTCGGAGGCCAGCTTGTTCGGGt       <  1:5282179/150‑1 (MQ=255)
                                                                                                                                                ccccGTCGGGCCCCCGCTTCGAAGACCAACGTGCGGGTTAGTGCACGTCCTTCCAGTACTCGCGCTTGAGGAGATAGGCGAATACGAAGAAGAAGGCCAGGTAGAGCAGGACGTAGGTACCGATACGCTCGGAGGCCAGCTTGTTCGGGt       <  1:4167675/150‑1 (MQ=255)
                                                                                                                                                ccccGTCGGGCCCCCGCTTCGAAGACCAACGTGCGGGTTAGTGCACGTCCTTCCAGTACTCGCGCTTGAGGAGATAGGCGAATACGAAGAAGAAGGCCAGGTAGAGCAGGACGTAGGTACCGATACGCTCGGAGGCCAGCTTGTTCGGGt       <  2:5026202/150‑1 (MQ=255)
                                                                                                                                                ccccGTAGGGCGCGCGCTTCGAAGACCAACGTGCGGGTTAGTGCACGTCCTTCCAGTACTCGCGCTTGAGGAGATAGGCGAATACGAAGAAGAAGGCCAGGTAGAGCAGGACGTAGGTACCGATACGCTCGGAGGCCAGCTTGTTCGGGt       >  1:2210643/1‑150 (MQ=255)
                                                                                                                                                  ccGTCGGGCGCGCGCTTCGAAGACCAACGTGCGGGTTAGTGCACGTCCTTCCAGTACTCGCGCTTGAGGAGATAGGCGAATACGAAGAAGAAGGCCAGGTAGAGCAGGACGTAGGTACCGATACGCTCGGAGGCCAGCTTGTTCGGGTTg     <  2:811437/150‑1 (MQ=255)
                                                                                                                                                  ccGTAGGGCGCGCGCTTCGAAGACCAACGTGCGGGTTAGTGCACGTCCTTCCAGTACTCGCGCTTGAGGAGATAGGCGAATACGAAGAAGAAGGCCAGGTAGAGCAGGACGTAGGTACCGATACGCTCGGAGGCCAGCTTGTTCGGGTTg     >  1:4449414/1‑150 (MQ=255)
                                                                                                                                                   cGTAGGGCGCGCGCTTCGAAGACCAACGTGCGGGTTAGTGCACGTCCTTCCAGTACTCGCGCTTGAGGAGATAGGCGAATACGAAGAAGAAGGCCAGGTAGAGCAGGACGTAGGTACCGATACGCTCGGAGGCCAGCTTGTTCGGGTTgg    >  2:9674235/1‑150 (MQ=255)
                                                                                                                                                    gTAGGGCGCGCGCTTCGAAGACCAACGTGCGGGTTAGTGCACGTCCTTCCAGTACTCGCGCTTGAGGAGATAGGCGAATACGAAGAAGAAGGCCAGGTAGAGCAGGACGTAGGTACCGATACGCTCGGAGGCCAGCTTGTTCGGGTTGGc   >  1:1900505/1‑150 (MQ=255)
                                                                                                                                                    gTAGGGCGCGCGCTTCGAAGACCAACGTGCGGGTTAGTGCACGTCCTTCCAGTACGCGCGCTTGAGGAGATAGGCGAATACGAAGAAGAAGGCCAGGTAGAGCAGGACGTAGGTACCGATCCGCTCGGAGGCCAGCTTGTGCGGGTTGGc   >  2:2103023/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TACCGCCACGTTCTTCTCGGCCAGGACCAGGCGGATGCGATGGCAGTAATGATCGGCAGGGTCGGAATAGCAGGTCAGCTTGTTGATTGAAGCCATGGAGCCCTCCTCGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGTAGGGCGCGCGCTTCGAAGACCAACGTGCGGGTTAGTGCACGTCCTTCCAGTACTCGCGCTTGAGGAGATAGGCGAATACGAAGAAGAAGGCCAGGTAGAGCAGGACGTAGGTACCGATACGCTCGGAGGCCAGCTTGTTCGGGTTGGCC  >  NC_008463/5125650‑5125948

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑