BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_008463	1,239,163	(C)_5→4	intergenic (+161/‑32)	PA14_RS05795 → / → lptF	alpha/beta hydrolase/LPS export ABC transporter permease LptF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_008463	1,239,159	0	C	.	100.0%	2025.8 / NA	408	intergenic (+157/‑36)	PA14_RS05795/lptF	alpha/beta hydrolase/LPS export ABC transporter permease LptF
Reads supporting (aligned to +/- strand): ref base C (0/0); new base . (184/224); total (184/224)

Only 100 of 422 total aligned reads displayed.

AGCGCTGAACGAGCGTTCGCCTCGACGGTCTATCGGGCTCTTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCNGACGGGCCCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGATCATCATGAGTGGCCGTTTCATCAAGTACCTGGCGCA  >  NC_008463/1239010‑1239304

 gcgcTGAACGAGCGTTCGCCTCGACGGTCTATCGGGCTCTTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGGcc                                                                                                                                                  >  2:5424368/1‑150 (MQ=255)

   gcTGAACGAGCGTTCGCCTCGACGGTCTATCGGGCTCTTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGGcccc                                                                                                                                                <  1:7678497/150‑1 (MQ=255)

   gcTGAACGAGCGTTCGCCTCGACGGTCTATCGGGCTCTTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGGcccc                                                                                                                                                >  1:7302574/1‑150 (MQ=255)

       aaCGAGCGTTCGCCTCGACGGTCTATCGGGCTCTTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGACACGCGTGATGACGGGGCCGACGGGccccgtca                                                                                                                                            >  1:8600556/1‑146 (MQ=255)

         cGAGCGTTCGCCTCGACGGTCTATCGGGCTCTTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCaga                                                                                                                                         <  2:2206501/150‑1 (MQ=255)

          gAGCGTTCGCCTCGACGGTCTATCGGGCTCTTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCagag                                                                                                                                        <  1:5084371/150‑1 (MQ=255)

              gTTCGCCTCGACGGTCTATCGGGCTCTTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTg                                                                                                                                    >  2:4771315/1‑150 (MQ=255)

               ttCGCCTCGACGGTCTATCGGGCTCTTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTgg                                                                                                                                   >  1:8090674/1‑150 (MQ=255)

                tCGCCTCGACGGTCTATCGGGCTCTTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGc                                                                                                                                  <  1:8816504/150‑1 (MQ=255)

                tCGCCACGACGGTCTATCGGGCTCTTGGTTCGGCCGGCGCAGTGACAGGCCCGCCGCATCGCAGGATACTGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGAGGACGGCGCCGACGGG‑CCCCGTCAGCGCCTGGc                                                                                                                                  <  1:3256714/150‑1 (MQ=255)

                   ccTCGACGGTCTATCGGGCTCTTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAAc                                                                                                                               <  1:10430251/150‑1 (MQ=255)

                    cTCGACGGTCTATCGGGCTCTTGGGTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAAcc                                                                                                                              <  1:10980998/150‑1 (MQ=255)

                       gACGGTCTATCGGGCTCTTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAg                                                                                                                           <  2:3594701/150‑1 (MQ=255)

                       gACGGTCTATCGGGCTCTTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAg                                                                                                                           >  1:2010056/1‑150 (MQ=255)

                        aCGGTCTATCGGGCTCTTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAgg                                                                                                                          <  1:7184411/150‑1 (MQ=255)

                          ggTCTATCGGGCTCTTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAg                                                                                                                        <  1:10033805/150‑1 (MQ=255)

                           gTCTATCGGGCTCTTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAgt                                                                                                                       >  1:3361118/1‑150 (MQ=255)

                           gTCTATCGGGCTCTTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAgt                                                                                                                       >  1:9272654/1‑150 (MQ=255)

                              tATCGGGCTCTTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTc                                                                                                                    <  1:6914081/150‑1 (MQ=255)

                                 cGGGCTCTTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCgg                                                                                                                 <  1:2466907/150‑1 (MQ=255)

                                    gCTCTTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGAtt                                                                                                              <  1:2324879/150‑1 (MQ=255)

                                      tctTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGa                                                                                                            <  1:5475752/150‑1 (MQ=255)

                                       ctTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGAt                                                                                                           <  1:3083543/150‑1 (MQ=255)

                                            ttCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGttt                                                                                                      <  1:7879974/150‑1 (MQ=255)

                                              cGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTc                                                                                                    <  1:4426789/150‑1 (MQ=255)

                                              cGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTc                                                                                                    <  2:8892292/150‑1 (MQ=255)

                                               ggCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTcc                                                                                                   <  1:1812377/150‑1 (MQ=255)

                                                gCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGACACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCg                                                                                                  >  1:534763/1‑150 (MQ=255)

                                                gCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGCGTGTCCGGATTGATCGTTTTCCg                                                                                                  >  2:10760960/1‑150 (MQ=255)

                                                gCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCg                                                                                                  >  1:3703657/1‑150 (MQ=255)

                                                 ccTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTACGCGCCCTGCCGCCATGGCCGGCAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGt                                                                                                 >  2:4718183/1‑150 (MQ=255)

                                                  cTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGtt                                                                                                <  1:6262712/150‑1 (MQ=255)

                                                  cTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGtt                                                                                                >  1:3766449/1‑150 (MQ=255)

                                                    gcgcAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAACCGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTGAt                                                                                              >  2:7429105/1‑150 (MQ=255)

                                                      gcAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTAttt                                                                                            <  1:8275721/150‑1 (MQ=255)

                                                      gcAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTAttt                                                                                            <  1:6790642/150‑1 (MQ=255)

                                                      gcAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTAttt                                                                                            <  1:1209494/150‑1 (MQ=255)

                                                      gcAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTAttt                                                                                            <  1:9390379/150‑1 (MQ=255)

                                                       cAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTg                                                                                           <  2:1819718/150‑1 (MQ=255)

                                                            aCAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCg                                                                                      <  2:10299034/150‑1 (MQ=255)

                                                               ggCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGa                                                                                   <  2:9232893/150‑1 (MQ=255)

                                                                gCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGaa                                                                                  <  1:7479594/150‑1 (MQ=255)

                                                                gCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGaa                                                                                  <  2:6666538/150‑1 (MQ=255)

                                                                 cccGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAg                                                                                 >  2:2093016/1‑150 (MQ=255)

                                                                 cccGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAg                                                                                 >  1:4915929/1‑150 (MQ=255)

                                                                 cccGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGGCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAg                                                                                 >  2:380124/1‑150 (MQ=255)

                                                                  ccgccgAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGt                                                                                >  2:11035313/1‑150 (MQ=255)

                                                                      cgAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTg                                                                            <  1:8547378/150‑1 (MQ=255)

                                                                       gAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTgg                                                                           >  1:2681720/1‑150 (MQ=255)

                                                                       gAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTgg                                                                           >  2:10359666/1‑150 (MQ=255)

                                                                         aacGCAGGATACTGCCGGCTATGGTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTc                                                                         <  2:5916580/148‑1 (MQ=255)

                                                                         aTCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTc                                                                         <  1:3522843/150‑1 (MQ=255)

                                                                           cGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCAc                                                                       >  2:8448312/1‑150 (MQ=255)

                                                                            gCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCAcc                                                                      <  1:1523812/150‑1 (MQ=255)

                                                                             cAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCa                                                                     >  2:9553662/1‑150 (MQ=255)

                                                                             cAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCa                                                                     <  1:4216312/150‑1 (MQ=255)

                                                                             cAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCa                                                                     >  2:1729327/1‑150 (MQ=255)

                                                                             cAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCa                                                                     >  1:576492/1‑150 (MQ=255)

                                                                              aGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCAt                                                                    >  2:9789278/1‑150 (MQ=255)

                                                                                 aTAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAg                                                                 >  2:5111106/1‑150 (MQ=255)

                                                                                  tAATGCCGGCTATTTTCTCCGCGCCATGCCGCCCCGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGCGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAgc                                                                >  2:4929432/1‑150 (MQ=255)

                                                                                   aaTGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAgcg                                                               >  2:7782093/1‑150 (MQ=255)

                                                                                      gCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCg                                                            >  1:1656349/1‑150 (MQ=255)

                                                                                        cGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTc                                                          >  1:7091354/1‑150 (MQ=255)

                                                                                          gCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAg                                                        >  2:3054822/1‑150 (MQ=255)

                                                                                           cTATTTTCTCCGCGCCATGCCGCCCTGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAgc                                                       <  2:5589534/150‑1 (MQ=255)

                                                                                            tATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAgcg                                                      <  2:1370018/150‑1 (MQ=255)

                                                                                            tATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAgcg                                                      >  1:4717097/1‑150 (MQ=255)

                                                                                             aTTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAgcgc                                                     >  2:2968563/1‑150 (MQ=255)

                                                                                               tttCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCg                                                   <  1:5885792/150‑1 (MQ=255)

                                                                                                 tctcCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGtg                                                 >  2:336833/1‑150 (MQ=255)

                                                                                                         ccATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGt                                         <  2:7876797/150‑1 (MQ=255)

                                                                                                          cATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTg                                        >  2:2563561/1‑150 (MQ=255)

                                                                                                               cgccATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGatcat                                   <  2:7012824/150‑1 (MQ=255)

                                                                                                                 ccATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGAtcatca                                 <  2:8593102/150‑1 (MQ=255)

                                                                                                                 ccATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGACCCTGGCAACCCAGGAGTGTCCGGATTGAGCGTTTACCGTTATTTGTCCCGAGAAGTGCTCGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGAtcatca                                 >  2:10739042/1‑150 (MQ=255)

                                                                                                                    tGGCCGGAACCGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGATCATCATGa                              >  1:11021115/1‑150 (MQ=255)

                                                                                                                     ggCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGATCATCATGAg                             <  1:8946255/150‑1 (MQ=255)

                                                                                                                     ggCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGATCATCATGAg                             <  1:10155232/150‑1 (MQ=255)

                                                                                                                     ggCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGATCATCATGAg                             <  2:402943/150‑1 (MQ=255)

                                                                                                                      gCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGATCATCATGAGt                            <  1:3982656/150‑1 (MQ=255)

                                                                                                                        cGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGATCATCATGAGTgg                          <  1:9086194/150‑1 (MQ=255)

                                                                                                                         ggAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGATCATCATGAGTGGc                         <  2:8757058/150‑1 (MQ=255)

                                                                                                                         ggAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGATCATCATGAGTGGc                         >  2:6394109/1‑150 (MQ=255)

                                                                                                                           aaaCGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGATCATCATGAGTGGCCg                       >  2:11177797/1‑150 (MQ=255)

                                                                                                                            aaCGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGATCATCATGAGTGGCCGt                      >  1:6832941/1‑150 (MQ=255)

                                                                                                                             aCGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGATCATCATGAGTGGCCGtt                     >  2:2785339/1‑150 (MQ=255)

                                                                                                                             aCGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGATCATCATGAGTGGCCGtt                     <  1:6403531/150‑1 (MQ=255)

                                                                                                                              cgcgTGATGACGGGGCCGGCGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGATCATCATGAGTGGCCGttt                    <  1:2518574/150‑1 (MQ=255)

                                                                                                                               gcgTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGATCATCATGAGTGGCCGTTtc                   >  1:3416346/1‑150 (MQ=255)

                                                                                                                                  tgatgaCGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGATCATCATGAGTGGCCGTTtcatc                >  1:5550692/1‑150 (MQ=255)

                                                                                                                                   gatgaCGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGATCATCATGAGTGGCCGTTtcatca               >  2:864533/1‑150 (MQ=255)

                                                                                                                                      gaCGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGATCATCATGAGTGGCCGTTTCATCAAGt            <  2:4455953/150‑1 (MQ=255)

                                                                                                                                         ggggCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGATCATCATGAGTGGCCGTTTCATCAAGTAcc         <  2:821061/150‑1 (MQ=255)

                                                                                                                                         ggggACGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTGGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGATCATCATGAGTGGCCGTTTCATCAAGTAcc         <  2:5725113/150‑1 (MQ=255)

                                                                                                                                          gggCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGATCATCATGAGTGGCCGTTTCATCAAGTACCt        <  1:7397399/150‑1 (MQ=255)

                                                                                                                                          gggCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGATCATCATGAGTGGCCGTTTCATCAAGTACCt        <  2:4194150/150‑1 (MQ=255)

                                                                                                                                            gCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGATCATCATGAGTGGCCGTTTCATCAAGTACCTgg      <  2:4557801/150‑1 (MQ=255)

                                                                                                                                               gACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGATCATCATGAGTGGCCGTTTCATCAAGTACCTGgcgc   >  2:6117509/1‑150 (MQ=255)

                                                                                                                                                aCGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGATCATCATGAGTGGCCGTTTCATCAAGTACCTGGCGCa  <  1:10516522/150‑1 (MQ=255)

AGCGCTGAACGAGCGTTCGCCTCGACGGTCTATCGGGCTCTTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCNGACGGGCCCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGATCATCATGAGTGGCCGTTTCATCAAGTACCTGGCGCA  >  NC_008463/1239010‑1239304

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`