Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 2,354,163 (G)5→4 intergenic (‑48/+3) PA14_RS10945 ← / ← lipA lipase secretion chaperone/triacylglycerol lipase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084632,354,1590G.99.7% 1862.1 / ‑2.5 377intergenic (‑44/+7)PA14_RS10945/lipAlipase secretion chaperone/triacylglycerol lipase
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base . (188/188);  total (189/188)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 403 total aligned reads displayed.

CGCTGGCCGGGGGCGCCGTCTCGGGTGCGGGGGAAGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAAGCCCCGGCCGGGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGTCCAGGTGGTTCATCCGGTAGTTGTCGCGGATCACCATG  >  NC_008463/2354010‑2354304
                                                                                                                                                     |                                                                                                                                                 
cgcTGGCCGGGGGCGCCGTCTCGGGTGCGGGGGAAGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCccggccg                                                                                                                                                   >  2:10468199/1‑150 (MQ=255)
cgcTGGCCGGGGGCGCCGTCTCGGGTGCGGGGGAAGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCccggccg                                                                                                                                                   >  1:4745445/1‑150 (MQ=255)
  cTGGCCGGGGGCGCCGTCTCGGGTGCGGGGGAAGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCCggg                                                                                                                                                 >  2:4026211/1‑150 (MQ=255)
    ggCCGGGGGCGCCGTCTCGGGTGCGGGGGAAGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCCggggt                                                                                                                                               >  1:8179106/1‑149 (MQ=255)
            gcgcCTTCTCGGGTGCGGGGGACGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCCGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAgg                                                                                                                                      <  1:489054/150‑1 (MQ=255)
             cgcCGTCTCGGGTGCGGGGGATGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGATCGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGc                                                                                                                                     <  2:9906606/150‑1 (MQ=255)
             cgcCGTCTCGGGTGCGGGGGAAGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGc                                                                                                                                     <  2:10870313/150‑1 (MQ=255)
             cgcCGTCTCGGGGGCGGGGGAAGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGc                                                                                                                                     <  1:8615632/150‑1 (MQ=255)
              gcCGTCTCGGGTGCGGGGGAAGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCt                                                                                                                                    >  1:9928582/1‑150 (MQ=255)
              gcCGTCTCGGGTGCGGGGGAAGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCt                                                                                                                                    <  2:6310896/150‑1 (MQ=255)
               ccGTCTCGGGTGCGGGGGAAGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTg                                                                                                                                   >  1:8784211/1‑150 (MQ=255)
                cGTCTCGGGTGCGGGGGAAGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTgg                                                                                                                                  >  2:10231406/1‑150 (MQ=255)
                  tctcGGGTGCGGGGGAAGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCg                                                                                                                                >  1:2845694/1‑150 (MQ=255)
                     cGGGTGCGGGGGAAGGTTCCAGCCAGACGAACCCGGCCAGGCGGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGttc                                                                                                                             <  1:10533678/150‑1 (MQ=255)
                      gggTGCGGGGGAAGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGATCGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGttct                                                                                                                            <  2:7145388/150‑1 (MQ=255)
                      gggTGCGGGGGAAGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGttct                                                                                                                            >  2:3436688/1‑150 (MQ=255)
                       ggTGCGGGGGAAGGTTCCCGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTGTCTTCACGCGAGGGGGCTTCCTGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGttctt                                                                                                                           <  2:5675794/150‑1 (MQ=255)
                       ggTGCGGGGGAAGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGttctt                                                                                                                           >  1:10100783/1‑150 (MQ=255)
                        gTGCGGGGGAAGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGttcttc                                                                                                                          >  1:4309449/1‑150 (MQ=255)
                          gCGGGGGAAGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAg                                                                                                                        >  2:4303678/1‑150 (MQ=255)
                           cGGGGGAAGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGTCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAgg                                                                                                                       <  2:7908500/150‑1 (MQ=255)
                            gggggAAGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGc                                                                                                                      >  2:227116/1‑150 (MQ=255)
                             ggggAAGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCg                                                                                                                     >  2:8342471/1‑150 (MQ=255)
                                gatggTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCGGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGtt                                                                                                                  <  1:10366044/147‑1 (MQ=255)
                                gAAGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGtt                                                                                                                  >  1:7369359/1‑150 (MQ=255)
                                 aaGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTg                                                                                                                 >  1:8294612/1‑150 (MQ=255)
                                 aaGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTg                                                                                                                 <  2:10052762/150‑1 (MQ=255)
                                       ccagccagACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGc                                                                                                           >  1:9326970/1‑150 (MQ=255)
                                        cagccagACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCt                                                                                                          <  1:7216505/150‑1 (MQ=255)
                                        cagccagACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCt                                                                                                          >  2:8064113/1‑150 (MQ=255)
                                           ccagACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGc                                                                                                       <  2:5141934/150‑1 (MQ=255)
                                             agACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCgg                                                                                                     <  1:398143/150‑1 (MQ=255)
                                                   aCCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACg                                                                                               >  1:10992442/1‑150 (MQ=255)
                                                    ccaggccaggCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGc                                                                                              >  2:4588225/1‑150 (MQ=255)
                                                     caggccaggcTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCt                                                                                             >  2:1219673/1‑150 (MQ=255)
                                                     caggccaggcTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCt                                                                                             >  2:6785231/1‑150 (MQ=255)
                                                       ggccaggcTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGa                                                                                           >  2:5443541/1‑150 (MQ=255)
                                                        gccaggcTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGAc                                                                                          >  2:6642849/1‑150 (MQ=255)
                                                         ccaggcTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGGGGGGGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGAcc                                                                                         <  1:3560572/150‑1 (MQ=255)
                                                          caggcTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCg                                                                                        <  1:5098706/150‑1 (MQ=255)
                                                          caggcTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCg                                                                                        <  2:6213926/150‑1 (MQ=255)
                                                           aggcTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCgg                                                                                       >  2:905342/1‑150 (MQ=255)
                                                            ggcTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCggg                                                                                      >  1:10210393/1‑150 (MQ=255)
                                                            ggcTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCggg                                                                                      >  1:5420779/1‑150 (MQ=255)
                                                                ggcggcGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTgg                                                                                  >  1:1198434/1‑150 (MQ=255)
                                                                   ggcgAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGtct                                                                               >  2:7359133/1‑150 (MQ=255)
                                                                       aaCGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGaa                                                                           >  1:6338641/1‑150 (MQ=255)
                                                                       aaCGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGaa                                                                           >  1:1746786/1‑150 (MQ=255)
                                                                         cGCCAGTGGACTCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACa                                                                         <  1:1463879/150‑1 (MQ=255)
                                                                          gCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAg                                                                        >  1:7090332/1‑150 (MQ=255)
                                                                           ccAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAgg                                                                       >  1:5196433/1‑150 (MQ=255)
                                                                            cAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGc                                                                      <  1:6739394/150‑1 (MQ=255)
                                                                             aGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCt                                                                     >  1:7698424/1‑150 (MQ=255)
                                                                                ggAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGt                                                                  >  1:244705/1‑150 (MQ=255)
                                                                                 gAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTg                                                                 >  2:10746302/1‑150 (MQ=255)
                                                                                  aaTCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGa                                                                >  1:7543550/1‑150 (MQ=255)
                                                                                   ataagcacGAGGATTTTCTTCACGCGAGGGGGCTTCCTGTAAAGGGCCTGGGCAGAGGCCCCGGCC‑GGGGTCCTACAGTCTGCCCTTCTTAAGTCGGTTGGCGAGCTGTCGGTAGACTATGACCGGGCTGGTCACTAACAGTCAGGagag                                                               >  2:7543336/4‑150 (MQ=255)
                                                                                   aTCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAg                                                               <  1:5837729/150‑1 (MQ=255)
                                                                                    tCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCTGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAgg                                                              >  2:6038801/1‑150 (MQ=255)
                                                                                     cagcagGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGc                                                             <  2:8533968/150‑1 (MQ=255)
                                                                                       gcagGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGCGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCg                                                           <  2:3810020/150‑1 (MQ=255)
                                                                                       gcagGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCg                                                           >  1:2272542/1‑150 (MQ=255)
                                                                                         aggaggATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGaa                                                         >  1:9015049/1‑150 (MQ=255)
                                                                                         aggaggATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGaa                                                         >  2:11135578/1‑150 (MQ=255)
                                                                                          ggaggaTTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAg                                                        <  2:133916/150‑1 (MQ=255)
                                                                                          ggaggaTTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAg                                                        <  2:10171510/150‑1 (MQ=255)
                                                                                            aggaTTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGAc                                                      >  1:9479787/1‑150 (MQ=255)
                                                                                               aTTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTg                                                   >  1:5585016/1‑150 (MQ=255)
                                                                                                ttttCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTgg                                                  >  2:9541901/1‑150 (MQ=255)
                                                                                                   tcttcACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTc                                               >  1:10725642/1‑150 (MQ=255)
                                                                                                       cACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCt                                           <  1:86972/150‑1 (MQ=255)
                                                                                                       cACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCt                                           <  1:7845507/150‑1 (MQ=255)
                                                                                                        ccgcgAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTc                                          <  2:9830425/149‑1 (MQ=255)
                                                                                                        aCGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTc                                          <  2:4233002/150‑1 (MQ=255)
                                                                                                         cgcgAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCg                                         >  2:5278971/1‑150 (MQ=255)
                                                                                                          gcgAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGt                                        >  1:5665712/1‑150 (MQ=255)
                                                                                                           cgAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGTc                                       >  2:9407783/1‑150 (MQ=255)
                                                                                                           cgAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGTc                                       >  2:3616396/1‑150 (MQ=255)
                                                                                                            gtgggggCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGTcc                                      <  1:659800/148‑1 (MQ=255)
                                                                                                            gAGGCGGCGTCCGGGAAAGGGCCGGTGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGGTCTTCAGGCGGGTGGCGAGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTTGTTCACCTCGTcc                                      <  2:10328402/150‑1 (MQ=255)
                                                                                                             aGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGTCCa                                     >  1:4510371/1‑150 (MQ=255)
                                                                                                              gggggCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGTCCAg                                    <  2:1500115/150‑1 (MQ=255)
                                                                                                               ggggCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGTCCAgg                                   >  2:7689821/1‑150 (MQ=255)
                                                                                                                     tCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGTCCAGGTGGTTc                             <  2:2490216/150‑1 (MQ=255)
                                                                                                                     tCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGTCCAGGTGGTTc                             >  1:3326521/1‑150 (MQ=255)
                                                                                                                      ccGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACCGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGTCCAGGTGGTTCa                            <  2:728532/150‑1 (MQ=255)
                                                                                                                              gggccggggccgAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGTCCAGGTGGTTCATCCGGtcg                    >  2:9584886/1‑148 (MQ=255)
                                                                                                                                 ccggggccgAGGCCCCGGCC‑GGGGTCCTCCAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGATGGTGAGGCCGAAGACCTGGTTCACCTCGTCCAGGTGGTTCATCCGGTAGTTg                 >  2:2690626/1‑150 (MQ=255)
                                                                                                                                 ccggggccgAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGTCCAGGTGGTTCATCCGGTAGTTg                 <  2:391378/150‑1 (MQ=255)
                                                                                                                                  cggggccgAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGTCCAGGTGGTTCATCCGGTAGTTGt                <  2:3227281/150‑1 (MQ=255)
                                                                                                                                   ggggccgAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGTCCAGGTGGTTCATCCGGTAGTTGTc               <  1:9748999/150‑1 (MQ=255)
                                                                                                                                    gggccgAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGTCCAGGTGGTTCATCCGGTAGTTGTcg              >  2:9262222/1‑150 (MQ=255)
                                                                                                                                    gggccgAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGGTCACCTCGTCCAGGTGGTTCATCCGGTAGTTGTcg              >  2:9754139/1‑150 (MQ=255)
                                                                                                                                      gccgAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGTCCAGGTGGTTCATCCGGTAGTTGTcgcg            >  1:5628677/1‑150 (MQ=255)
                                                                                                                                         gaggCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGTCCAGGTGGTTCATCCGGTAGTTGTCGCGGAt         >  1:1246897/4‑150 (MQ=255)
                                                                                                                                         gaggCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGTCCAGGTGGTTCATCCGGTAGTTGTCGCGGAt         >  1:4411661/4‑150 (MQ=255)
                                                                                                                                         gaggCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGTCCAGGTGGTTCATCCGGTAGTTGTCGCGGAt         <  2:4909420/147‑1 (MQ=255)
                                                                                                                                              cccGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCTGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCTCCTCGTACAGGTGGTTCATCCGGTAGTTGTCGCGGATCACCa    <  1:8629693/150‑1 (MQ=255)
                                                                                                                                              cccGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGTCCAGGTGGTTCATCCGGTAGTTGTCGCGGATCACCa    >  1:1627037/1‑150 (MQ=255)
                                                                                                                                               ccggcc‑ggGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGGTCACCTCGTCCAGGTGGTTCATCCGGGAGTTGGCGCGGATCACCAt   >  2:1822484/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                 
CGCTGGCCGGGGGCGCCGTCTCGGGTGCGGGGGAAGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAAGCCCCGGCCGGGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGTCCAGGTGGTTCATCCGGTAGTTGTCGCGGATCACCATG  >  NC_008463/2354010‑2354304

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑