BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_008463	5,131,729	Δ34 bp	pseudogene (288‑321/1180 nt)	PA14_RS23490 ←	acyl‑CoA dehydrogenase family protein

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_008463	5131729	5131762	34	655 [0]	[1] 649	PA14_RS23490	acyl‑CoA dehydrogenase family protein

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_008463	= 5131728	0 (0.000)	571 (1.280)	205/262	0.1	100%	pseudogene (322/1180 nt)	PA14_RS23490	acyl‑CoA dehydrogenase family protein
*	?	NC_008463	5131763 =	0 (0.000)	571 (1.280)	205/262	0.1	100%	pseudogene (287/1180 nt)	PA14_RS23490	acyl‑CoA dehydrogenase family protein

Only 100 of 649 total aligned reads displayed.

CCCGGCTCGGTCATGGCGATGGCCGCGACCATCTCGCCGGATACCAGCTTCGGCAGGTACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGGCGCAGCGATGTCCGAATGTAGGGCGCAGCGATGTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATGACCACGGCGCTGTAGAGGAAGTCCGCGCCCATCCCGCCATACTCTTCCGGCAGGTGCGAACAGAGCATTCCGGCCTCGCCGGCCTTGTTCCAG  >  NC_008463/5131618‑5131912

cgtggtctccaccccttgcaggtcggaaccggcccccggctcggtcatggcgatggccgcgaccatctcgccggataccagcttcggcaggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGa                                                                                                                                                   >  2:96350‑M2/146‑150 (MQ=255)

 gtggtcttcaccccttgcaggtcggaaccggcccccggctcggtcatggcgatggccgcgaccatctcgccggataccagcttcggcaggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGaa                                                                                                                                                  <  2:10761173‑M2/6‑1 (MQ=255)

 gtggtcttcaccccttgcaggtcggaaccggcccccggctcggtcatggcgatggccgcgaccatctcgccggataccagcttcggcaggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGaa                                                                                                                                                  <  2:9473722‑M2/6‑1 (MQ=255)

  tggtcttcaccccttgcaggtcggaaccggcccccggctcggtcatggcgatggccgcgaccatctcgccggataccagcttcggcaggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAAt                                                                                                                                                 <  2:5557532‑M2/7‑1 (MQ=255)

  tggtcttcaccccttgcaggtcggaaccggcccccggctcggtcatggcgatggccgcgaccatctcgccggataccagcttcggcaggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAAt                                                                                                                                                 <  2:7155350‑M2/7‑1 (MQ=255)

  tggtcttcaccccttgcaggtcggaaccggcccccggctcggtcatggcgatggccgcgaccatctcgccggataccagcttcggcaggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAAt                                                                                                                                                 >  1:4427225‑M2/144‑150 (MQ=255)

   ggtcttcaccccttgcaggtcggaaccggcccccggctcggtcatggcgatggccgcgaccatctcgccggataccagcttcggcaggtacttgcgcttcagcgcctcgctgccggagtgggggatgtagggcgcagcgatgTCCGAATg                                                                                                                                                >  2:4779201‑M2/143‑150 (MQ=255)

     tcttcaccccttgcaggtcggaaccggcccccggctcggtcatggcgatggccgcgaccatctcgccggataccagcttcggcaggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCa                                                                                                                                              >  1:1296371‑M2/141‑150 (MQ=255)

         caccccttgcaggtcggaaccggcccccggctcggtcatggcgatggccgcgaccatctcgccggataccagcttcggcaggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGga                                                                                                                                          <  2:4591030‑M2/14‑1 (MQ=255)

          accccttgcaggtcggaaccggcccccggctcggtcatggcgatggccgcgaccatctcgccggataccagcttcggcaggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGgag                                                                                                                                         <  2:1626416‑M2/15‑1 (MQ=255)

             ccttgcaggtcggaaccggcccccggctcggtcatggcgatggccgcgaccatctcgccggataccagcttcggcaggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGaaa                                                                                                                                      <  1:8221109‑M2/18‑1 (MQ=255)

               ttgcaggtcggaaccggcccccggctcggtcatggcgatggccgcgaccatctcgccggataccagcttcggcaggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAAcc                                                                                                                                    >  2:6160425‑M2/131‑150 (MQ=255)

               ttgcaggtcggaaccggcccccggctcggtcatggcgatggccgcgaccatctcgccggataccagcttcggcaggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAAcc                                                                                                                                    <  2:2886808‑M2/20‑1 (MQ=255)

               ttgcaggtcggaaccggcccccggctcggtcatggcgatggccgcgaccagctcgccggataccagcttcggcaggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGACAcc                                                                                                                                    <  2:4312203‑M2/20‑1 (MQ=255)

                 gcaggtcggaaccggcccccggctcggtcatggcgatggccgcgaccatctcgccggataccagcttcggcaggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGa                                                                                                                                  <  1:8920967‑M2/22‑1 (MQ=255)

                        ggaaccggcccccggctcggtcatggcgatggccgcgaccatctcgccggataccagcttcggcaggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGt                                                                                                                           <  1:8691879‑M2/29‑1 (MQ=255)

                            ccggcccccggctcggtcatggcgatggccgcgaccatctcgccggataccagcttcggcaggtacttgcgcctcagcgcctcgctgccggagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAgg                                                                                                                       >  1:2487496‑M2/118‑150 (MQ=255)

                             cggcccccggctcggtcatggcgatggccgcgaccatctcgccggataccagcttcggcaggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAggc                                                                                                                      >  2:8981753‑M2/117‑150 (MQ=255)

                                cccccggctcggtcatggcgatggccgcgaccatctcgccggataccagcttcggcaggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAggccgg                                                                                                                   <  2:925617‑M2/37‑1 (MQ=255)

                                  cccggctcggtcatggcgatggccgcgaccatctcgccggataccagcttcggcaggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAggccggcc                                                                                                                 >  1:4543575‑M2/112‑150 (MQ=255)

                                   ccggctcggtcatggcgatggccgcgaccatctcgccggataccagcttcggcaggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGccc                                                                                                                <  2:6803544‑M2/40‑1 (MQ=255)

                                         cggtcatggcgatggccgcgaccatctcgccggataccagcttcggcaggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCccgaccga                                                                                                          <  1:7160333‑M2/46‑1 (MQ=255)

                                           gtcatggcgatggccgcgaccatctcgccggataccagcttcggcaggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGAtc                                                                                                        >  2:4160413‑M2/103‑150 (MQ=255)

                                           gtcatggcgatggccgcgaccatctcgccggataccagcttcggcaggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGAtc                                                                                                        >  1:1490152‑M2/103‑150 (MQ=255)

                                           gtcatggcgatggccgcgaccatctcgccggataccagcttcggcaggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGAtc                                                                                                        >  1:206294‑M2/103‑150 (MQ=255)

                                             catggcgatggccgcgaccatctcgccggataccagcttcggcaggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGAtctc                                                                                                      >  1:6298553‑M2/101‑150 (MQ=255)

                                               tggcgatggccgcgaccatctcgccggataccagcttcggcaggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCtt                                                                                                    >  1:4861803‑M2/99‑150 (MQ=255)

                                               tggcgatggccgcgaccatctcgccggataccagcgtcggcaggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCtt                                                                                                    <  2:7648839‑M2/52‑1 (MQ=255)

                                               tggccatggccgcgaccaactcgccggatccccgccgcggcaggtacttgcgcttccgcgccacgctgccggagtggaggctgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCtt                                                                                                    <  2:5809703‑M2/52‑1 (MQ=255)

                                                   gatggccgcgaccatctcgccgtataccagcttcggcaggtacttgcgattcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGAt                                                                                                >  2:6433738‑M2/95‑150 (MQ=255)

                                                   gatggccgcgaccatctcgccggataccagcttcggcaggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGAt                                                                                                <  1:79835‑M2/56‑1 (MQ=255)

                                                     tggccgcgaccatctcgccggataccagcttcggcaggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATGa                                                                                              >  2:3070248‑M2/93‑150 (MQ=255)

                                                       gccgcgaccatctcgccggataccagcttcggcaggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATGAcc                                                                                            <  1:8585274‑M2/60‑1 (MQ=255)

                                                            gaccatctcgccggataccagcttcggcaggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATGACCACGgc                                                                                       <  2:3158623‑M2/65‑1 (MQ=255)

                                                              ccatctcgccggataccagcttcggcaggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATGACCACGgcgc                                                                                     >  1:5646810‑M2/84‑150 (MQ=255)

                                                                atctcgccggataccagcttcggcaggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATGACCACGGCGCTg                                                                                   <  1:4759023‑M2/69‑1 (MQ=255)

                                                                    cgccggataccagcttcggcaggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATGACCACGGCGCTGTaga                                                                               <  1:5196736‑M2/73‑1 (MQ=255)

                                                                    cgccggagaccagcatcggcaggtcctagcgcttccgcgcctcgctgccgtaggggcggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGAACCCGCTCAGGCCGGCCCGACCGAACACTTCCATGACCACGGCGCCGTaga                                                                               <  2:10670175‑M2/73‑1 (MQ=255)

                                                                       cggataccagcttcggcaggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATGACCACGGCGCTGTAGAGGa                                                                            >  2:3686777‑M2/75‑150 (MQ=255)

                                                                       cggataccagcttcggcaggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATGACCACGGCGCTGTAGAGGa                                                                            >  1:7422402‑M2/75‑150 (MQ=255)

                                                                       cggataccagcttcggcaggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATGACCACGGCGCTGTAGAGGa                                                                            >  1:9902944‑M2/75‑150 (MQ=255)

                                                                        ggataccagcttcggcaggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATGACCACGGCGCTGTAGAGGaa                                                                           <  1:1616302‑M2/77‑1 (MQ=255)

                                                                        ggataccagcttcggcaggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATGACCACGGCGCTGTAGAGGaa                                                                           >  1:5475651‑M2/74‑150 (MQ=255)

                                                                         gataccagcttcggcaggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATGACCACGGCGCTGTAGAGGAAg                                                                          >  2:352966‑M2/73‑150 (MQ=255)

                                                                          ataccagcttcggcaggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATGACCACGGCGCTGTAGAGGAAGt                                                                         >  1:3209824‑M2/72‑150 (MQ=255)

                                                                           taccagcttcggcaggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATGACCACGGCGCTGTAGAGGAAGTc                                                                        <  1:7836369‑M2/80‑1 (MQ=255)

                                                                            accagcttcggcaggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATGACCACGGCGCTGTAGAGGAAGTcc                                                                       <  2:10886472‑M2/81‑1 (MQ=255)

                                                                             ccagcttcggcaggtacttgcgcttcagcgcctcgctgccgtagtggaggaggtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATGACCACGGCGCTGTAGAGGAAGTCcg                                                                      >  2:977315‑M2/69‑150 (MQ=255)

                                                                              cagcttcggcaggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATGACCACGGCGCTGTAGAGGAAGTCcgc                                                                     <  2:4021777‑M2/83‑1 (MQ=255)

                                                                              cagcttcggcaggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATGACCACGGCGCTGTAGAGGAAGTCcgc                                                                     <  1:4071285‑M2/83‑1 (MQ=255)

                                                                                gcttcggcaggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATGACCACGGCGCTGTAGAGGAAGTCCgcgc                                                                   >  2:5721178‑M2/66‑150 (MQ=255)

                                                                                 cttcggcaggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATGACCACGGCGCTGTAGAGGAAGTCCGCGcc                                                                  >  2:8418811‑M2/65‑150 (MQ=255)

                                                                                 cttcggcaggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATGACCACGGCGCTGTAGAGGAAGTCCGCGcc                                                                  >  1:1778657‑M2/65‑150 (MQ=255)

                                                                                  ttcggcaggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATGACCACGGCGCTGTAGAGGAAGTCCGCGccc                                                                 >  2:6986106‑M2/64‑150 (MQ=255)

                                                                                  ttcggcaggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATGACCACGGCGCTGTAGAGGAAGTCCGCGccc                                                                 >  1:6525971‑M2/64‑150 (MQ=255)

                                                                                    cggcaggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATGACCACGGCGCTGTAGAGGACGTCCGCGCCCAt                                                               >  2:10288080‑M2/62‑150 (MQ=255)

                                                                                    cggcaggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATGACCACGGCGCTGTAGAGGAAGTCCGCGCCCAt                                                               <  1:771366‑M2/89‑1 (MQ=255)

                                                                                     ggcaggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATGACCACGGCGCTGTAGAGGAAGTCCGCGCCCATc                                                              >  2:810851‑M2/61‑150 (MQ=255)

                                                                                     ggcaggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATGACCACGGCGCTGTAGAGGAAGTCCGCGCCCATc                                                              <  2:233239‑M2/90‑1 (MQ=255)

                                                                                      gcaggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATGACCACGGCGCTGTAGAGGAAGTCCGCGCCCATcc                                                             <  2:8288205‑M2/91‑1 (MQ=255)

                                                                                      gcaggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATGACCACGGCGCTGTAGAGGAAGTCCGCGCCCATcc                                                             <  1:7270719‑M2/91‑1 (MQ=255)

                                                                                        aggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATGACCACGGCGCTGTAGAGGAAGTCCGCGCCCATCCCg                                                           <  1:10930041‑M2/93‑1 (MQ=255)

                                                                                         ggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATGACCACGGCGCTGTAGAGGAAGTCCGCGCCCATCCCGc                                                          >  1:4308760‑M2/57‑150 (MQ=255)

                                                                                          gtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATGACCACGGCGCTGTAGAGGAAGTCCGCGCCCATCCCGcc                                                         <  1:3422646‑M2/95‑1 (MQ=255)

                                                                                             cttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATGACCACGGCGCTGTAGAGGAAGTCCGCGCCCATCCCGCCATa                                                      >  1:3139998‑M2/53‑150 (MQ=255)

                                                                                               tgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATGACCACGGCGCTGTAGAGGAAGTCCGCGCCCATCCCGCCATAct                                                    >  1:1125565‑M2/51‑150 (MQ=255)

                                                                                               tgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATGACCACGGCGCTGTAGAGGAAGTCCGCGCCCATCCCGCCATAct                                                    >  1:5412937‑M2/51‑150 (MQ=255)

                                                                                                gcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATGACCACGGCGCTGTAGAGGAAGTCCGCGCCCATCCCGCCATActc                                                   >  1:10042922‑M2/50‑150 (MQ=255)

                                                                                                gcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATGACCACGGCGCTGTAGAGGAAGTCCGCGCCCATCCCGCCATActc                                                   <  1:2792604‑M2/101‑1 (MQ=255)

                                                                                                 cgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATGACCACGGCGCTGTAGAGGAAGTCCGCGCCCATCCCGCCAAActct                                                  >  1:9186081‑M2/49‑150 (MQ=255)

                                                                                                  gcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGACGGCCCGACCGATCTCTTCGATGACCACGGCGCTGTAGAGGAAGTCCGCGCCCATCCCGCCATACTCtt                                                 <  1:2617043‑M2/103‑1 (MQ=255)

                                                                                                   cttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATGACCACGGCGCTGTAGAGGAAGTCCGCGCCCATCCCGCCATACTCTTc                                                <  2:5107032‑M2/104‑1 (MQ=255)

                                                                                                       agcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATGACCACGGCGCTGTAGAGGAAGTCCGCGCCCATCCCGCCATACTCTTCCGGc                                            >  2:8500313‑M2/43‑150 (MQ=255)

                                                                                                       agcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATGACCACGGCGCTGTAGAGGAAGTCCGCGCCCATCCCGCCATACTCTTCCGGc                                            >  1:5908746‑M2/43‑150 (MQ=255)

                                                                                                       agcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATGACCACGGCGCTGTAGAGGAAGTCCGCGCCCATCCCGCCATACTCTTCCGGc                                            >  1:1057553‑M2/43‑150 (MQ=255)

                                                                                                        gcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATGACCACGGCGCTGTAGAGGAAGTCCGCGCCCATCCCGCCATACTCTTCCGGCa                                           >  1:6696812‑M2/42‑150 (MQ=255)

                                                                                                            ctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATGACCACGGCGCTGTAGAGGAAGTCCGCGCCCATCCCGCCATACTCTTCCGGCAGGTg                                       <  1:9793481‑M2/113‑1 (MQ=255)

                                                                                                             tcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCCAACCCGGTCAGGCCGGCCCGACCGATCTCTTCGATGACCACGGCGCTGTAGAGGAAGTCCGCGCCCATCCCGCCATACTCTTCCGGCAGGTGc                                      >  2:7402044‑M2/37‑150 (MQ=255)

                                                                                                               gctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATGACCACGGCGCTGTAGAGGAAGTCCGCGCCCATCCCGCCATACTCTTCCGGCAGGTGCGa                                    <  2:1148198‑M2/116‑1 (MQ=255)

                                                                                                               gctgccggagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATGACCACGGCGCTGTAGAGGAAGTCCGCGCCCATCCCGCCATACTCTTCCGGCAGGTGCGa                                    >  2:9862282‑M2/35‑150 (MQ=255)

                                                                                                                ctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATGACCACGGCGCTGTAGAGGAAGTCCGCGCCCATCCCGCCATACTCTTCCGGCAGGTGCGaa                                   >  2:2688287‑M2/34‑150 (MQ=255)

                                                                                                                 tgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATGACCACGGCGCTGTAGAGGAAGTCCGCGCCCATCCCGCCATACTCTTCCGGCAGGTGCGAAc                                  <  1:9575730‑M2/118‑1 (MQ=255)

                                                                                                                   ccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATGACCACGGCGCTGTAGAGGAAGTCCGCGCCCATCCCGCCATACTCTTCCGGCAGGTGCGACCag                                >  2:7975396‑M2/31‑150 (MQ=255)

                                                                                                                   ccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATGACCACGGCGCTGTAGAGGAAGTCCGCGCCCATCCCGCCATACTCTTCCGGCAGGTGCGAACag                                <  1:10200296‑M2/120‑1 (MQ=255)

                                                                                                                   ccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATGACCACGGCGCTGTAGAGGAAGTCCGCGCCCATCCCGCCATACTCTTCCGGCAGGTGCGAACag                                <  2:9057216‑M2/120‑1 (MQ=255)

                                                                                                                   ccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATGACCACGGCGCTGTAGAGGAAGTCCGCGCCCATCCCGCCATACTCTTCCGGCAGGTGCGAACag                                <  1:3812890‑M2/120‑1 (MQ=255)

                                                                                                                    cgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATGACCACGGCGCTGTAGAGGAAGTCCGCGCCCATCCCGCCATACTCTTCCGGCAGGTGCGAACaga                               <  2:1493678‑M2/121‑1 (MQ=255)

                                                                                                                     gtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATGACCACGGCGCTGTAGAGGAAGTCCGCGCCCATCCCGCCATACTCTTCCGGCAGGTGCGAACagag                              <  2:4919829‑M2/122‑1 (MQ=255)

                                                                                                                      tagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATGACCACGGCGCTGTAGAGGAAGTCCGCGCCCATCCCGCCATACTCTTCCGGCAGGTGCGAACAGAGc                             >  2:1706409‑M2/28‑150 (MQ=255)

                                                                                                                      tagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATGACCACGGCGCTGTAGAGGAAGTCCGCGCCCATCCCGCCATACTCTTCCGGCAGGTGCGAACAGAGc                             <  1:1742893‑M2/123‑1 (MQ=255)

                                                                                                                           gaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATGACCACGGCGCTGTAGAGGAAGTCCGCGCCCATCCCGCCATACTCTTCCGGCAGGTGCGAACAGAGCATTcc                        <  1:3623449‑M2/128‑1 (MQ=255)

                                                                                                                             ggatgtagggcgcagcgatgTCCGAATGCAGGGAGACACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATGACCACGGCGCTGTAGAGGAAGTCCGCGCCCATCCCGCCATACTCTTCCGGCAGGTGCGCACAGAGCATTCCgg                      >  2:6702944‑M2/21‑150 (MQ=255)

                                                                                                                                  tagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATGACCACGGCGCTGTAGAGGAAGTCCGCGCCCATCCCGCCATACTCTTCCGGCAGGTGCGAACAGAGCATTCCGGCCTCg                 <  2:8313600‑M2/135‑1 (MQ=255)

                                                                                                                                    gggcgcagcgaggTCCGAATGCAGGGCGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCGTCGATGACCACGGCGCTGTAGAGGAAGTCCGCGCCCATCCCGCCATACTCTTGCGGCAGGTGCGAACAGAGCTTTCCGGCCTCGcc               >  2:5469895‑M2/14‑150 (MQ=255)

                                                                                                                                     ggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATGACCACGGCGCTGTAGAGGAAGTCCGCGCCCATCCCGCCATACTCTTCCGGCAGGTGCGAACAGAGCATTCCGGCCTCGCCg              <  2:2043061‑M2/138‑1 (MQ=255)

                                                                                                                                     ggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATGACCACGGCGCTGTAGAGGAAGTCCGCGCCCATCCCGCCATACTCTTCCGGCAGGTGCGAACAGAGCATTCCGGCCTCGCCg              >  1:3480078‑M2/13‑150 (MQ=255)

                                                                                                                                          agcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATGACCACGGCGCTGTAGAGGAAGTCCGCGCCCATCCCGCCATACTCTTCCGGCAGGTGCGAACAGAGCATTCCGGCCTCGCCGGCCtt         <  2:8819173‑M2/143‑1 (MQ=255)

                                                                                                                                           gcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATGACCACGGCGCTGTAGAGGAAGTCCGCGCCCATCCCGCCATACTCTTCCGGCAGGTGCGAACAGAGCATTCCGGCCTCGCCGGCCTTg        >  1:5032390‑M2/7‑150 (MQ=255)

                                                                                                                                            cgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATGACCACGGCGCTGTAGAGGAAGTCCGCGCCCATCCCGCCATACTCTTCCGGCAGGTGCGAACAGAGCATTCCGGCCTCGCCGGCCTTGt       <  2:212336‑M2/145‑1 (MQ=255)

                                                                                                                                              atgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATGACCACGGCGCTGTAGAGGAAGTCCGCGCCCATCCCGCCATACTCTTCCGGCAGGTGCGAACAGAGCATTCCGGCCTCGCCGGCCTTGTTc     >  1:2201898‑M2/4‑150 (MQ=255)

CCCGGCTCGGTCATGGCGATGGCCGCGACCATCTCGCCGGATACCAGCTTCGGCAGGTACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGGCGCAGCGATGTCCGAATGTAGGGCGCAGCGATGTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATGACCACGGCGCTGTAGAGGAAGTCCGCGCCCATCCCGCCATACTCTTCCGGCAGGTGCGAACAGAGCATTCCGGCCTCGCCGGCCTTGTTCCAG  >  NC_008463/5131618‑5131912

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`