Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084635,125,7990TG24.8% 386.9 / 23.8 228intergenic (‑54/+32)PA14_RS23455/PA14_RS23460glutathione S‑transferase N‑terminal domain‑containing protein/cytochrome c1
Reads supporting (aligned to +/- strand):  ref base T (109/58);  new base G (44/11);  total (153/75)
Fisher's exact test for biased strand distribution p-value = 4.47e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 231 total aligned reads displayed.

TACCGCCACGTTCTTCTCGGCCAGGACCAGGCGGATGCGATGGCAGTAATGATCGGCAGGGTCGGAATAGCAGGTCAGCTTGTTGATTGAAGCCATGGAGCCCTCCTCGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGTAGGGCGCGCGCTTCGAAGACCAACGTGCGGGTTAGTGCACGTCCTTCCAGTACTCGCGCTTGAGGAGATAGGCGAATACGAAGAAGAAGGCCAGGTAGAGCAGGACGTAGGTACCGATACGCTCGGAGGCCAGCTTGTTCGGGTTGGCC  >  NC_008463/5125650‑5125948
                                                                                                                                                     |                                                                                                                                                     
tACCGCCACGTTCTTCTCGGCCAGGACCAGGCGGATGCGATGGCAGTAATGATCGGCAGGGTCGGAATAGCAGGTCAGCTTGTTGATTGAAGCCATGGAGCCCTCCTCGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGt                                                                                                                                                       >  1:7287432/1‑150 (MQ=255)
 aCCGCCACGTTCTTCTCGGCCAGGACCAGGCGGATGCGATGGCAGTAATGATCGGCAGGGTCGGAATAGCAGGTCAGCTTGTTGATTGAAGCCATGGAGCCCTCCTCGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGTa                                                                                                                                                      <  2:4777456/150‑1 (MQ=255)
 aCCGCCACGTTCTTCTCGGCCAGGACCAGGCGGATGCGATGGCAGTAATGATCGGCAGGGTCGGAATAGCAGGTCAGCTTGTTGATTGAAGCCATGGAGCCCTCCTCGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGTa                                                                                                                                                      <  2:5314165/150‑1 (MQ=255)
  ccGCCACGTTCTTCTCGGCCAGGACCAGGCGGATGCGATGGCAGTAATGATCGGCAGGGTCGGAATAGCAGGTCAGCTTGTTGATTGAAGCCATGGAGCCCTCCTCGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGTAg                                                                                                                                                     <  1:6093804/150‑1 (MQ=255)
  ccGCCACGTTCTTCTCGGCCAGGACCAGGCGGATGCGATGGCAGTAATGATCGGCAGGGTCGGAATAGCAGGTCAGCTTGTTGATTGAAGCCATGGAGCCCTCCTCGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGTAg                                                                                                                                                     <  1:4306679/150‑1 (MQ=255)
  ccGCCACGTTCTTCTCGGCCAGGACCAGGCGGATGCGATGGCAGTAATGATCGGCAGGGTCGGAATAGCAGGTCAGCTTGTTGATTGAAGCCATGGAGCCCTCCTCGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGTAg                                                                                                                                                     <  1:7852994/150‑1 (MQ=255)
    gCCACGTTCTTCTCGGCCAGGACCAGGCGGATGCGATGGCAGTAATGATCGGCAGGGTCGGAATAGCAGGTCAGCTTGTTGATTGAAGCCATGGAGCCCTCCTCGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGTAggg                                                                                                                                                   <  1:9089427/150‑1 (MQ=255)
    gCCACGTTCTTCTCGGCCAGGACCAGGCGGATGCGATGGCAGTAATGATCGGCAGGGTCGGAATAGCAGGTCAGCTTGTTGATTGAAGCCATGGAGCCCTCCTCGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGTAggg                                                                                                                                                   >  2:6171067/1‑150 (MQ=255)
     ccACGTTCTTCTCGGCCAGGACCAGGCGGATGCGATGGCAGTAATGATCGGCAGGGTCGGAATAGCAGGTCAGCTTGTTGATTGAAGCCATGGAGCCCTCCTCGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGTAGGgc                                                                                                                                                  <  1:1850088/150‑1 (MQ=255)
      cACGTTCTTCTCGGCCAGGACCAGGCGGATGCGATGGCAGTAATGATCGGCAGGGTCGGAATAGCAGGTCAGCTTGTTGATTGAAGCCATGGAGCCCTCCTCGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGTTGGgcg                                                                                                                                                 <  2:6512507/150‑1 (MQ=255)
      cACGTTCTTCTCGGCCAGGACCAGGCGGATGCGATGGCAGTAATGATCGGCAGGGTCGGAATAGCAGGTCAGCTTGTTGATTGAAGCCATGGAGCCCTCCTCGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGTAGGgcg                                                                                                                                                 >  2:10037456/1‑150 (MQ=255)
      cACGTTCTTCTCGGCCAGGACCAGGCGGATGCGATGGCAGTAATGATCGGCAGGGTCGGAATAGCAGGTCAGCTTGTTGATTGAAGCCATGGAGCCCTCCTCGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGTAGGgcg                                                                                                                                                 <  2:11017365/150‑1 (MQ=255)
       aCGTTCTTCTCGGCCAGGACCAGGCGGATGCGATGGCAGTAATGATCGGCAGGGTCGGAATAGCAGGTCAGCTTGTTGATTGAAGCCATGGAGCCCTCCTCGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGGAGGgcgc                                                                                                                                                >  2:6066794/1‑150 (MQ=255)
           tcttctCGGCCTGGACCTGGGGGATGCTTTGGCATTAATTATCGGCTGGGTCGGAATAGCTGGTCAGATTGTTTATTTAAGCCTTGGAGCCCTCCTCGAAGGTCGACTTGTGGGAGGTTAAAAGACGCGCGCGCCCCGTCGGgcgcgcgc                                                                                                                                            <  1:2924981/150‑1 (MQ=255)
              tctcGGCCAGGACCAGGCGGATGCGATGGCAGTAATGATCGGCAGGGTCGGAATAGCAGGTCAGCTTGTTGATTGAAGCCATGGAGCCCTCCTCGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGTAGGGCGCGCGCTTc                                                                                                                                         >  2:1324602/1‑150 (MQ=255)
                tcGGCCAGGACCAGGCGGATGCGATGGCAGTAATGATCGGCAGGGTCGGAATAGCAGGTCAGCTTGTTGATTGAAGCCATGGAGCCCTCCTCGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGTAGGGCGCGCGCTTCGa                                                                                                                                       >  1:9620715/1‑150 (MQ=255)
                    ccAGGACCAGGCGGATGCGATGGCAGTAATGATCGGCAGGGTCGGAATAGCAGGTCAGCTTGTTGATTGAAGCCATGGAGCCCTCCTCGCAGGTCGACTTGTGGGAGGGGAAAAGACGCGCGCGCCCCGTAGGGCGCGCGCTTCGAAGAc                                                                                                                                   >  2:5747343/1‑150 (MQ=255)
                        gACCAGGCGGATGCGATGGCAGTAATGATCGGCAGGGTCGGAATAGCAGGTCAGCTTGTTGATTGAAGCCATGGAGCCCTCCTCGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGTAGGGCGCGCGCTTCGAAGACCAAc                                                                                                                               >  1:2422268/1‑150 (MQ=255)
                        gACCAGGCGGATGCGATGGCAGTAATGATCGGCAGGGTCGGAATAGCAGGTCAGCTTGTTGATTGAAGCCATGGAGCCCTCCTCGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCACGTAGGGCGCGCGCTTCGAATACCAAc                                                                                                                               >  2:7406088/1‑150 (MQ=255)
                          ccAGGCGGATGCGATGGCAGTAATGATCGGCAGGGTCGGAATAGCAGGTCAGCTTGTTGATTGAAGCCATGGAGCCCTCCTCGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGGAGGGCGCGCGCGTCGAAGACCAACGt                                                                                                                             >  1:8834170/1‑150 (MQ=255)
                           cAGGCGGATGCGATGGCAGTAATGATCGGCAGGGTCGGAATAGCAGGTCAGCTTGTTGATTGAAGCCATGGAGCCCTCCTCGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGTAGGGCGCGCGCTTCGAAGACCCACGTg                                                                                                                            >  2:10426730/1‑150 (MQ=255)
                               cGGATGCGATGGCAGTAATGATCGGCAGGGTCGGAATAGCAGGTCAGCTTGTTGATTGAAGCCATGGAGCCCTCCTCGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGTAGGGCGCGCGCTTCGAAGACCAACGTGCggg                                                                                                                        >  2:934584/1‑150 (MQ=255)
                                 gATGCGATGGCAGTAATGATCGGCAGGGTCGGAATAGCAGGTCAGCTTGTTGATTGAAGCCATGGAGCCCTCCTCGCAGGTCGACGTGTGGGAGGTGAAAAGACGCGCGCGCCCCGTAGGGCGCGCGCTTCGAAGACCAACGTGCggggt                                                                                                                      >  1:9224085/1‑148 (MQ=255)
                                       aTGGCAGTAATGATCGGCAGGGTCGGAATAGCAGGTCAGCTTGTTGATTGAAGCCATGGAGCCCTCCTCGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGTAGGGCGCGCGCTTCGAAGACCAACGTGCGGGTTAGTGCa                                                                                                                >  1:9247688/1‑150 (MQ=255)
                                        tGGCAGTAATGATCGGCAGGGTCGGAATAGCAGGTCAGCTTGTGGATTGAAGCCAGGGAGCCCTCCTCGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGTAGGGCGCGCGGTTCGGAGACCAACGTGGGGGTTAGTGGAc                                                                                                               >  1:10489408/1‑150 (MQ=255)
                                         ggCAGTAATGATCGGCAGGGTCGGAATAGCAGGTCAGCTTGTTGATTGAAGCCATGGAGCCCTCCTCGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGTAGGGCGCGCCCTTAGAAGACCAAAGTTCGGGTTAGTGCACg                                                                                                              >  1:9709239/1‑150 (MQ=255)
                                         ggCAGTAATGATCGGCAGGGTCGGAATAGCAGGTCAGCGTGTTGATTGAAGCCATGGAGCCCTCCTCGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGTAGGGCGCGCGCTTCGAAGACCAACGTGGGGGGTAGTGCACg                                                                                                              >  2:10398626/1‑150 (MQ=255)
                                            aGTAATGATCGGCAGGGTCGGAATAGCAGGTCAGCTTGTTGATTGAAGCCATGGAGCCCTCCTCGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGGAGGGCGCGCGCTTCGAAGACCAACGTGCGGGTTAGTGCACGTcc                                                                                                           >  1:3602261/1‑150 (MQ=255)
                                             gTAATGATCGGCAGGGTCGGAATAGCAGGTCAGCTTGTTGATTGAAGCCATGGAGCCCTCCTCGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGGAGGGCGCGCGCTTCGAAGACCAACGTGCGGGTTAGTGCACGTCCt                                                                                                          >  2:989302/1‑150 (MQ=255)
                                               aattacgggCTGGGTCGTTATAGCAGGTCTGCTTGTTGATTTACCCCTTGCTCCCAGCCCCCCTGGTCTATGTGGGGGTGGTTAAATGCCCCGCCCCCCCCGTTGGGCGCCCGCTTCGAAGACCAACGTGCGGGTTAGTGCACGTCCTTc                                                                                                        <  2:2519409/143‑1 (MQ=255)
                                                 tGATCGGCAGGGTCGGAATAGCAGGTCAGCTTGTTGATTGAAGCCATGGAGCCCTCCTCGCAGGTCGACTGGTGGGAGGTGAAAAGACGCGCGCGCCCCGTAGGGCGCGCGCTTGGTAGAACAACGGGCGGGTTAGTGCACGTCCTTCCa                                                                                                      >  1:4494715/1‑150 (MQ=255)
                                                     cGGCAGGGTCGGAATAGCAGGTCAGCTTGTTGATTGAAGCCATGGAGCCCTCCTCGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGGATGGCGCGCGCATAGGAAAACAACGTGCGGGTTAGTGCACGTACGTACAGTAc                                                                                                  >  1:9519952/1‑150 (MQ=255)
                                                      ggCAGGGTCGGAATAGCAGGTCAGCTTGTTGATTGAAGCCATGGAGCCCTCCTCGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGGAGGGCGCGCGCGTCGAAGACCAACGTGCGGGGGAGTGCACGTCCTTCCAGTACt                                                                                                 >  1:2319906/1‑150 (MQ=255)
                                                      ggCAGGGTCGGAATAGCAGGTCAGCTTGTTGATTGAAGCCATGGAGCCCTCCTCGCAGGTCGACGTGTGGGAGGTGAAAAGACGCGCGCGCCCCGTAGGGCGCGCGCTTCGAAGACCAACGTGCGGGTTAGTGCACGTCCTTCCAGGACt                                                                                                 >  2:4282003/1‑150 (MQ=255)
                                                        cAGGGTCGGAATAGCAGGTCAGCTTGTTGATTGAAGCCATGGAGCCCTCCTCGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGGAGGGCGCGCGCTTCGAAGACCCACCTGCGGGTTAGTGCACGTCCATCCAGTACTcg                                                                                               >  1:1991993/1‑150 (MQ=255)
                                                        cAGGGTCGGAATAGCAGGTCAGCTTGTTGATTGAAGCCATGGAGCCCTCCTCGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGGAGGGCGCGCGCTTCGAAGACCAACGTGCGGGTTAGTGCACGTCCTTCCAGTACTcg                                                                                               >  2:10503596/1‑150 (MQ=255)
                                                        cAGGGTCGGAATAGCAGGTCAGCTTGTTGATTGAAGCCATGGAGCCCTCCTCGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGGAGGGCGCGCGCGTCGAACACCAACCTGCGGGGTAGTGCACCTCCATACAGTACTcg                                                                                               >  1:7599095/1‑150 (MQ=255)
                                                         aGGGTCGGAATAGCAGGTCAGCTTGTTGATTGAAGCCATGGAGCCCTCCTCGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGTAGGGCGCGCGCTTCGAAGACCAACGTGCGGGTTAGTGCACGTCCTTCCAGTACgcgc                                                                                              >  2:4028819/1‑150 (MQ=255)
                                                         aGGGTCGGAATAGCAGGTCAGCTTGTTGATTGAAGCCATGGAGCCCTCCTCGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGTAGGGCGCGCGCTTCGAAGACCAACGTGCGGGTTAGTGCACGTCCTTCCAGTACTcgc                                                                                              >  2:9849102/1‑150 (MQ=255)
                                                           ggTCGGAATAGCAGGTCAGCTTGTTGATTGAAGCCATGGAGCCCTCCTCGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGTAGGGCGCGCGCTTCGAATACCAACGGGCGGGTTAGTGCACGTCCTTCCAGTACTCgcgc                                                                                            >  2:3093941/1‑150 (MQ=255)
                                                            gTCGGAATAGCAGGTCAGCTTGTTGATTGAAGCCATGGAGCCCTCCTCGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGTAGGGCGCGCGCTTCGAAGACCAACGTGCGGGTTAGTGCACGTCCTTCCAGTACTCGCGCt                                                                                           >  2:2893734/1‑150 (MQ=255)
                                                             tCGGAATAGCAGGTCAGCTTGTTGATTGAAGCCATGGAGCCCTCCTCGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGTAGGGCGCGCGCTTAGAAGACCAAAGTGCGGGGTAGTGCACGTCCTTCCAGTACTCGCGCtt                                                                                          >  1:10650709/1‑150 (MQ=255)
                                                              cGGAATAGCAGGTCAGCTTGTTGATTGAAGCCATGGAGCCCTCCTCGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGGAGGGCGCGCGCTTCGAATACCAACGTGCGGGGTAGTGCACGTCCTTCCAGTACTCGCGCTTg                                                                                         >  1:8320351/1‑150 (MQ=255)
                                                                tcatcgCAGGTCAGGTTGTTTATTCAAGCCTTGGAGCCCACCACGCTGGACTACTTTGGGTTGGGTAAACTCCGGGCGCCCCCCTTCGGGCGCGCGCTTCGAAGACCAACGTGCGGGTTAGTGCACGTCCTTCCAGTACTCGCGCTTgag                                                                                       <  2:6101344/145‑1 (MQ=255)
                                                                  aTAGCAGGTCAGCTTGTTGATTGAAGCCATGGAGCCCTCCTCGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGTAGGGCGCGCGCTTCGAAGACCAACGTGCGGGTTAGTGCACGTCCTTCCAGTACACGCGCTTgagga                                                                                     >  2:4697371/1‑150 (MQ=255)
                                                                     gCAGGTCAGCTTGTTGATTGAAGCCATGGAGCCCTCCTCGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGTAGGGCGCGCGCTTAGAAGACCAACCTGCGGGTTAGTGCACCTCCATCCAGTACTCGCGCGTgaggagtt                                                                                  >  1:8671513/1‑148 (MQ=255)
                                                                     gCAGGTCAGCTTGTTGATTGAAGCCATGGAGCCCTCCTCGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGGAGGGCGCGCGCTTCGAAGTCCAAAGTGCGGGTTAGTGCACGTCCTTCCAGTACTCGCGCGTGTGGGGAt                                                                                  >  1:9766871/1‑150 (MQ=255)
                                                                     gCAGGTCAGCTTGTTGATTGAAGCCATGGAGCCCTCCTCGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGGAGGGCGCGCGCTTCGAAGACCAACGTGCGGGTTAGTGCACGTCCTTCCAGTACTCGCGCTTGAGGAGAt                                                                                  >  2:10752988/1‑150 (MQ=255)
                                                                           cAGCTTGTTGATTGAAGCCATGGAGCCCTCCTCGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGTAGGGCGCGCGCGTAGAAGACCAACGTGCGGGTTAGTGCACGTCCTTCCAGGACTCGCGCGTGAGGCGTTAGGCGa                                                                            >  1:8043555/1‑150 (MQ=255)
                                                                             gCTTGTTGATTGAAGCCATGGAGCCCTCCTCGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCCCCCCGTAGGGCGCGCGCGTAGAAGACCAACCTGCGGGTTAGTGCACGTCCATCCAGTACTCGCGCGTGTGGAGATAGGCGaaa                                                                          >  1:9468481/1‑149 (MQ=255)
                                                                               ttgttgATTGAAGCCATGTAGCCCTCCTCGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGTGGGGCGCGCGCGTAGAAGACCAACGTGCGGGTTAGTGAACGTCCTTCCAGTAATCGCGCGTGAGGAGATAGGCGAATAc                                                                        >  1:2594656/1‑150 (MQ=255)
                                                                               ttgttgATTGAAGCCATGGAGCCCTCCTCGCAGGTCGACTTCAGGGAGGTGAAAAGACGCGCGCGCCCCGTAGGGCGCGCGCTTCGAAGACCAACGTGCGGGTTAGTGCACGTCCTTCCAGTACTCGCGCTTGAGGAGATAGGCGAATAc                                                                        >  2:9199385/1‑150 (MQ=255)
                                                                               tgtttgttttaAGCATTGGCGCCCGCCGCGCAGTGCGACTTGGGGGTGGTTAAAAGACGCGCGCGCCCCGGCGGGCGCGCGCTTCGAAGACCAACGTGCGGGTTAGTGCACGTCCTTCCAGTACTCGCGCTTGAGGAGATAGGCGAATAc                                                                        <  2:8755303/147‑1 (MQ=255)
                                                                                tgttgATTGAAGCCATGGAGCCCTCCTCGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGTAGGGCGCGCGCTTCGAATACCAACGTGCGGGTTATTGCACGTCCTTCCAGTACACGCGCTTGAGGAGATAGGCGAATACg                                                                       >  1:11143259/1‑150 (MQ=255)
                                                                                tgttgATTGAAGCCATGGAGCCCTCCTCGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGTAGGGCGCGCGCTTCGAAGACCAACGTGCGGGTTAGTGCACGTCCTTCCAGTACTCGCGCTTGAGGAGATAGGCGAATACg                                                                       >  2:10802872/1‑150 (MQ=255)
                                                                                  ttgattgaAGCCATGGAGCCCTCCTCGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGTAGGGCGCGCGCTTCGAAGACCAACGTGCGGGTTAGTGCACGTCCATCCAGTACTCGCGCTTGAGGAGATAGGCGAATACgaa                                                                     >  1:8084522/1‑150 (MQ=255)
                                                                                      ttgaAGCCATGGAGCCCTCCTCGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGTAGGGCGCGCGCTTCGAAGACCAACGTGCGGGGTAGTGCACGTCCTTCCAGTACGCGCGCGTGAGGAGATAGGCGAATaagaagaag                                                                 >  1:3389025/1‑150 (MQ=255)
                                                                                        gaAGCCATGGAGCCCTCCTCGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGTAGGGCGCGCTCTTCGAAGACCAACGTGCGGGTTAGTGCACGTCCTTCCAGTACTCGCGCTTGAGGAGATAGGCGAATACgaagaagaa                                                               <  2:8511522/150‑1 (MQ=255)
                                                                                          tgcctgggAGCCCTCCTCGCTGGTCGAGTTGTGGGTGGTTAAACGACGCGCGCGCCCCGTAGGGCGCGCGCTTCGAAGACCAACGTGCGGGTTAGTGCACGTCCTTCCAGTACTCGCGCTTGAGGAGATAGGCGAATACGAAGAAGAAgg                                                             <  2:7143960/144‑1 (MQ=255)
                                                                                          aGCCATGGAGCCCTCCTCGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGTAGGGCGCGCGCGTCGAAGACCAACGTGCGGGTTAGTGCACGTCCTTACAGTACTCGCGCTTGAGGAGATAGGCGAATACGAAGAAGAAgg                                                             >  2:5386578/1‑150 (MQ=255)
                                                                                           gCCATGGAGCCCTCCTCGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGTAGGGCGCGCGCTTCGAAGACCAACGTGCGGGTTAGTGCACGTCCATCCAGTACACGCGCTTGAGGAGATAGGCGAATACGAATAAGAATGc                                                            >  2:2591149/1‑150 (MQ=255)
                                                                                             cATGGAGCCCTCCTCGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGGAGGGCGCGCGCTTCGAAGAACAACGTGCGGGATAGTTCAAGTCCTTCCAGACCTCGCGCATGAGGAGATAGCCGAATAAgaaaaagaatgaca                                                          >  2:7647408/1‑145 (MQ=255)
                                                                                              aTGGAGCCCTCCTCGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCCGTGGGCGCGCGCTTAGTAGACCAACCTGCGGGTTGGTTCACGTCCTTCCAGTAATCGCGCTTGAGGAGTTATGCGAAAACGATGAAGGAGGCCAg                                                         >  1:3226764/1‑150 (MQ=255)
                                                                                              aTGGAGCCCTCCTCGCAGGTCGACTTGGGGGAGGTGAAAAGACGCGCGCGCCCCGGCGGGCGCGCGCTTCGCAGACCAACGTGCGGGTTAGTGCACGTGCGTCCAGTACTCGCGCTTGAGGAGATAGGCGAATACGAAGAAGTAGGCCAg                                                         >  2:6801521/1‑150 (MQ=255)
                                                                                                ggAGCCCTCCGCGCTGGTCGATTTGTGGTTGTTTAAATCACGCGCGCGCCCGGTTGGGCGCGCGCTTCGAAGACCAACGTGCGGGTTAGTGCACGTCCTTCCAGTACTCGCGCTTGAGGAGATAGGCGAATACGAAGAAGAAGGCCAGGt                                                       <  2:5674994/150‑1 (MQ=255)
                                                                                                   gCCCTCCTCGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGGAGGGCGCGCGCTTCGAAGACCAACGGGCGGGTTAGTGCACGTCCGTCCAGTACTCGCGCTTGTGGAGATAGGCGAATACGAAGAAGAAGGCCAGGTaga                                                    >  1:9144298/1‑150 (MQ=255)
                                                                                                       tcctcGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGGAGGGCGCGCGCGTCGAAGAGCCACGTGCGGGGGAGTGCACGTCCTTCCAGGACTCGCGCGGGAGGAGATAGGCGAATAAGAAGAAGTAGGCCAGGGAGAGCAg                                                >  1:270864/1‑150 (MQ=255)
                                                                                                         ctcGCTGGTCGATTTGTGGTTGGTTAAACGACGCGCGCGCCCCGGTGGGCGCCCGCTTCGAAGACCAACGTGCGGGTTAGTGCACGTCCTTCCAGTACTCGCGCTTGAGGAGATAGGCGAATACGAAGAAGAAGGCCAGGTAGAGCAGGa                                              <  2:7049414/150‑1 (MQ=255)
                                                                                                         ctcGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGTAGGGCGCGCGCTTCGAAGACCAACGTGCGGGTTATTGCACGTCCTTCCAGTACTCGCGCTTGAGGAGATAGGCGCATACGAAGAAGAAGGCCAGGTAGAGCAGGa                                              >  1:4228647/1‑150 (MQ=255)
                                                                                                          tcGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGTAGGGCGCGCGCGTCGAAGACCAACGTGCGGGGTAGTGCACCTCCATACACTACTCGCGCGTGTGGAGATAGGCGAATTAGAAGAATTATGCCAGGTAGAGCAGGAc                                             >  1:5188421/1‑150 (MQ=255)
                                                                                                             cAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCCGAGGGCGCGCCCTTCGAAGACCAACTTGAGGGTTAATGCACGTCAATCCAGTACAAGCGCGTTGGGAGATAGGCGAAAACGAAGAAGTAGGACCGGTAGAGCAAGAAGTa                                          >  2:4133681/1‑150 (MQ=255)
                                                                                                              aGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGTAGGGCGCGCGCTTCGAAGACCAACGTGCGGGTTAGTGCACGTCCTTCCAGTACTCGCGCTTGAGGAGATAGGCGAATACGAAGAAGAAGGCCAGGTAGAGCAGGACGTAg                                         >  2:7881350/1‑150 (MQ=255)
                                                                                                              aGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGTAGGGCGCGCGCTTAGAACACCAACGGGCGGGTTAGTGCACGTCCTTACAGTACTCGCGCGTGAGGAGATAGGCGAATACGAATAAGAAGGCCAGGTAGAGCAGGACGTAg                                         >  2:6540190/1‑150 (MQ=255)
                                                                                                                ttcgttttGTGGGAGGTTAAACGACGCGCGCGCCCCGTGGGGCGCGCGCTTCGAAGACCAACGTGCGGGTTAGTGCACGTCCTTCCAGTACTCGCGCTTGAGGAGATAGGCGAATACGAAGAAGAAGGCCAGGTAGAGCAGGACGTAGGt                                       <  2:11084946/144‑1 (MQ=255)
                                                                                                                  cGACTTGTGGGTGGTTAAAAGACGCGCCCGCCCCGTCGGGCGCCCGCTTCGAAGACCAACGTGCGGGTTAGTGCACGTCCTTCCAGTACTCGCGCTTGAGGAGATAGGCGAATACGAAGAAGAAGGCCAGGTAGAGCAGGACGTAGGTAc                                     <  2:9797063/150‑1 (MQ=255)
                                                                                                                    aCTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGTAGGGCGCGCGCTTCGAAGACCAACCTGCGGGTTAGTGCACGTCCATCCAGTACACGCGCTTGAGGAGATAGGCGAAAAAGAAGAAGAAGGCCAGGTATAGCAGGACGTAGGTACCg                                   >  1:7353507/1‑150 (MQ=255)
                                                                                                                     cTTGGGGGAGTGTAAATGCCGCGCGCCCCCCGATGGGCGCCCGCTTCGAAGACCAACGTGCGGGTTAGTGCACGTCCTTCCAGTACTCGCGCTTGAGGAGATAGGCGAATACGAAGAAGAAGGCCAGGTAGAGCAGGACGTAGGTACCGa                                  <  2:9105726/150‑1 (MQ=255)
                                                                                                                      ttGTGGGAGGTCACACGACGCGCCCCCCCCTTCGGCCCCCCGCTTCGAAGACCAACGTGCGGGTTAGTGCACGTCCTTCCAGTACTCGCGCTTGAGGAGATAGGCGAATACGAAGAAGAAGGCCAGGTAGAGCAGGACGTAGGTACCGAt                                 <  2:1989589/150‑1 (MQ=255)
                                                                                                                        gtgGGAGGTGAAAAGACGCGCGCGCCCCGTAGGGCGCGCGCTTCGAAGACCAACGTGCGGGTTAGTGCACGTCCTTCCAGTACTCGCGCTTGAGGAGATAGGCGAATACGAAGAAGAAGGCCCGGTTGAGCAGGACGTAGGTACCGATAc                               >  2:748022/1‑150 (MQ=255)
                                                                                                                          gggAGGTGAAAAGACGCGCGCGCCCCCGAGGGCGCGCGCGTAGAAGAACAACGTGCGGGTTAGTGCACGTACTTCCACTACACGCGCGTGAGGAGATAGGCGAAAACGAAGAACAATGCCAGGTAGAGCAGGACGTAGGTACCCATACGc                             >  1:7060710/1‑150 (MQ=255)
                                                                                                                           ggtggTTAAACGCCGCGCGCCCCCCGTGGGGCGCGCGCTTCGAAGACCAACGTGCGGGTTAGTGCACGTCCTTCCAGTACTCGCGCTTGAGGAGATAGGCGAATACGAAGAAGAAGGCCAGGTAGAGCAGGACGTAGGTACCGATACGCt                            <  2:5243634/147‑1 (MQ=255)
                                                                                                                            gtggTGAAATTCCGCGCGCGCCCGCTTGGCCGCGCGCTTCGAAGACCAACGTGCGGGTTAGTGCACGTCCTTCCAGTACTCGCGCTTGAGGAGATAGGCGAATACGAAGAAGAAGGCCAGGTAGAGCAGGACGTAGGTACCGATACGCTc                           <  2:2308280/148‑1 (MQ=255)
                                                                                                                              gttcaaaaCACGCCCGCCCCCCGCCGGCCCCCCGCTTCGAAGACCAACGTGCGGGTTAGTGCACGTCCTTCCAGTACTCGCGCTTGAGGAGATAGGCGAATACGAAGAAGAAGGCCAGGTAGAGCAGGACGTAGGTACCGATACGCTCgg                         <  1:3478910/146‑1 (MQ=255)
                                                                                                                                    acgccgcgccccCCCCGGCGGCCGCCCGCTTCGAAGACCAACGTGCGGGTTAGTGCACGTCCTTCCAGTACTCGCGCTTGAGGAGATAGGCGAATACGAAGAAGAAGGCCAGGTAGAGCAGGACGTAGGTACCGATACGCTCGGAGGCCa                   <  1:4835970/146‑1 (MQ=255)
                                                                                                                                    aaGCCGCGCGCCCCCCGGTGGGCGCGCGCTTCGAAGACCAACGTGCGGGTTAGTGCACGTCCTTCCAGTACTCGCGCTTGAGGAGATAGGCGAATACGAAGAAGAAGGCCAGGTAGAGCAGGACGTAGGTACCGATACGCTCGGAGGCCa                   <  2:3961349/150‑1 (MQ=255)
                                                                                                                                     cgACCCGCGCGCCCGCCGGGGCCCCCGCTTCGAAGACCAACGTGCGGGTTAGTGCACGTCCTTCCAGTACTCGCGCTTGAGGAGTTCGGCGAATACGAAGAAGAAGGCCAGGTAGAGCAGGACGTAGGTACCGATACGCTCGGAGGCCAg                  <  1:8192556/149‑1 (MQ=255)
                                                                                                                                      gccgcgcgccCCCCGACGGGCGCCCGCTTCGAAGACCAACGTGCGGGTTAGTGCACGTCCTTCCAGTACTCGCGCTTGAGGAGATAGGCGAATACGAAGAAGAAGGCCAGGTAGAGCAGGACGTAGGTACCGATACGCTCGGAGGCCAGc                 <  2:4991858/148‑1 (MQ=255)
                                                                                                                                       ccgcgcgccCCCCGGCGGCCCCCCGCTTCGAAGACCAACGTGCGGGTTAGTGCACGTCCTTCCAGTACTCGCGCTTGAGGAGATAGGCGAATACGAAGAAGAAGGCCAGGTAGAGCAGGACGTAGGTACCGATACGCTCGGAGGCCAGCt                <  1:6540167/149‑1 (MQ=255)
                                                                                                                                       aCGCGCGCGCCCGGGCGGCCGCGCGCTTCGAAGACCAACGTGCGGGTTAGTGCACGTCCTTCCAGTACTCGCGCTTGAGGAGATAGGCGAATACGAAGAAGAAGGCCAGGTAGAGCAGGACGTAGGTACCGATACGCTCGGAGGCCAGCt                <  2:5915118/150‑1 (MQ=255)
                                                                                                                                       aCGCGCGCGCCCCGTAGGGCGCGCGCTTCGAAGACCAACGTGCGGGTTAGTGCACGTCCTTCCAGTACTCGCGCTTGAGGAGATAGGCGAATACGAAGAAGAAGGCCAGGTAGAGCAGGACGTAGGTACCGATTCGCTCGGAGGCCAGCt                >  2:10555608/1‑150 (MQ=255)
                                                                                                                                        cgcgcgcgCCCCGTAGGGCGCGCGCTTCGAAGACCAACGTGCGGGTTAGTGCACGTCCTTCCAGTACTCGCGCTTGAGGAGATAGGCGAATACGAAGAAGAAGGCCAGGTAGAGCAGGACGTAGGTACCGATACGCTCGGAGGCCAGCtt               >  2:7865622/1‑150 (MQ=255)
                                                                                                                                          cccgcgcCCCGTCGGGCCCCCGCTTCGAAGACCAACGTGCGGGTTAGTGCACGTCCTTCCAGTACTCGCGCTTGAGGAGATAGGCGAATACGAAGAAGAAGGCCAGGTAGAGCAGGACGTAGGTACCGATACGCTCGGAGGCCAGCTTGt             <  2:8392892/148‑1 (MQ=255)
                                                                                                                                           gcgcccCCCTTTGGGCCCCCGCTTCGAAGACCAACGTGCGGGTTAGTGCACGTCCTTCCAGTACTCGCGCTTGAGGAGATAGGCGAATACGAAGAAGAAGGCCAGGTAGAGCAGGACGTAGGTACCGATACGCTCGGAGGCCAGCTTGtt            <  2:5864261/150‑1 (MQ=255)
                                                                                                                                            cgcgcCCCGTCGGGCGCCCGCTTCGAAGACCAACGTGCGGGTTAGTGCACGTCCTTCCAGTACTCGCGCTTGAGGAGATAGGCGAATACGAAGAAGAAGGCCAGGTAGAGCAGGACGTAGGTACCGATACGCTCGGAGGCCAGCTTGTTc           <  2:3420579/150‑1 (MQ=255)
                                                                                                                                             gcccccgGTAGGGCCCCCGCTTCGAAGACCAACGTGCGGGTTAGTGCACGTCCTTCCAGTACTCGCGCTTGAGGAGATAGGCGAATACGAAGAAGAAGGCCAGGTAGAGCATGAATTTGGTACCGATTAGCTCGTATGACTTCTTGTTCg          <  2:3810990/147‑1 (MQ=255)
                                                                                                                                                ccccGTAGGGCGCGCGCTTCGAAGACCAACGTGCGGGTTAGTGCACGTCCTTCCAGTACTCGCGCTTGAGGAGATAGGCGAATACGAAGAAGAAGGCCAGGTAGAGCAGGACGTAGGTACCGATACGCTCGGAGGCCAGCTTGTTCGGGt       >  1:2869017/1‑150 (MQ=255)
                                                                                                                                                  ccGTAGGGCGCGCGCTTCGAAGACCAACGTGCGGGTTAGTGCACGTCCTTCCAGTACTCGCGCTTGAGGAGATAGGCGAATACGAAGAAGAAGGCCAGGTAGAGCAGGACGTAGGTACCGATACGCTCGGAGGCCAGCTTGTTCGGGTTg     >  2:266701/1‑150 (MQ=255)
                                                                                                                                                  ccGTAGGGCGCGCGCTTCGAAGACCAACGTGCGGGTTAGTGCACGTCCTTCCAGTACTCGCGCTTGAGGAGATAGGCGAATACGAAGAAGAAGGCCAGGTAGAGCAGGACGTAGGTACCGATACGCTCGGAGGCCAGCTTGTTCGGGTTg     >  1:1243759/1‑150 (MQ=255)
                                                                                                                                                   cGTAGGGCGCGCGCTTCGAAGACCAACGTGCGGGTTAGTGCACGTCCTTCCAGTACTCGCGCTTGAGGAGATAGGCGAATACGAAGAAGAAGGCCAGGTAGAGCAGGACGTAGGTACCGATACGCTCGGAGGCCAGCTTGTTCGGGTTgg    >  1:1205961/1‑150 (MQ=255)
                                                                                                                                                    gTAGGGCGCGCGCTTCGAAGACCAACGTGCGGGTTAGTGCACGTCCTTCCAGTACTCGCGCTTGAGGAGATAGGCGAATACGAAGAAGTAGGCCAGGTAGAGCAGGACGTAGGTACCGATACGCTCGGAGGCCAGCTTGTTCGGGTTGGc   >  1:672681/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TACCGCCACGTTCTTCTCGGCCAGGACCAGGCGGATGCGATGGCAGTAATGATCGGCAGGGTCGGAATAGCAGGTCAGCTTGTTGATTGAAGCCATGGAGCCCTCCTCGCAGGTCGACTTGTGGGAGGTGAAAAGACGCGCGCGCCCCGTAGGGCGCGCGCTTCGAAGACCAACGTGCGGGTTAGTGCACGTCCTTCCAGTACTCGCGCTTGAGGAGATAGGCGAATACGAAGAAGAAGGCCAGGTAGAGCAGGACGTAGGTACCGATACGCTCGGAGGCCAGCTTGTTCGGGTTGGCC  >  NC_008463/5125650‑5125948

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑