Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084635,910,2560AC30.1% 576.7 / 42.7 295intergenic (+55/‑90)aceE/aceFpyruvate dehydrogenase (acetyl‑transferring), homodimeric type/dihydrolipoyllysine‑residue acetyltransferase
Reads supporting (aligned to +/- strand):  ref base A (142/62);  new base C (3/85);  total (145/150)
Fisher's exact test for biased strand distribution p-value = 5.59e-29
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 303 total aligned reads displayed.

TGGCCGATCGCGGCGATATCGAGCCGAAAGTGGTGGCCGAGGCCATCGCCAAGTA‑CGGTATCGATCCGGAGAAGCGCAACCCGCTGGATTGCTAATTCGCTCCATA‑CTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCAACGGGCGGCGCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGTAAAAGGAGAGGAATGTGAGCGAACTCATTCGCGTACCCGACATCGGCAACGGTGAGGGTGAAGTCATCGAGCTG  >  NC_008463/5910107‑5910405
                                                                                                                                                       |                                                                                                                                                     
tGGCCGATCGCGGCGATATCGAGCCGAAAGTGGTGGCCGAGGCCATCGCCAAGTA‑CGGTATCGATCCGGAGAAGCGCAACCCGCTGGATTGCTAATTCGCTCCATA‑CTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCaa                                                                                                                                                       >  2:7267184/1‑150 (MQ=255)
 ggCCGATCGCGGCGATATCGAGCCGAAAGTGGTGGCCGAGGCCATCGCCAAGTA‑CGGTATCGATCCGGAGAAGCGCAACCCGCTGGATTGCTAATTCGCTCCATA‑CTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCCAc                                                                                                                                                      >  1:10313570/1‑150 (MQ=255)
  gCCGATCGCGGCGATATCGAGCCGAAAGTGGTGGCCGAGGCCATCGCCAAGTA‑CGGTATCGATCCGGAGAAGCGCAACCCGCTGGATTGCTAATTCGCTCCATA‑CTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCCACg                                                                                                                                                     >  1:2991797/1‑150 (MQ=255)
  gCCGATCGCGGCGATATCGAGCCGAAAGTGGTGGCCGAGGCCATCGCCAAGTA‑CGGTATCGATCCGGAGAAGCGCAACCCGCTGGATTGCTAATTCGCTCCATA‑CTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCAACg                                                                                                                                                     >  2:10037658/1‑150 (MQ=255)
   ccGATCGCGGCGATATCGAGCCGAAAGTGGTGGCCGAGGCCATCGCCAAGTA‑CGGTATCGATCCGGAGAAGCGCAACCCGCTGGATTGCTAATTCGCTCCATA‑CTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCAACgg                                                                                                                                                    <  2:9479635/150‑1 (MQ=255)
     gATCGCGGCGATATCGAGCCGAAAGTGGTGGCCGAGGCCATCGCCAAGTA‑CGGTATCGATCCGGAGAAGCGCAACCCGCTGGATTGCTAATTCGCTCCATA‑CTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCCACGggc                                                                                                                                                  >  1:6705576/1‑150 (MQ=255)
      tgcgcgGCGATATCGAGCCGAAAGTGGTGGCCGAGGCCATCGCCAAGTA‑CGGTATCGATCCGGAGAAGCGCAACCCGCTGGATTGCTAATTCGCTCCATA‑CTCCCCTCGGCAGCGCGGGGGTTCGAGCTCGCCGGCGCGGCCAACggggg                                                                                                                                                 >  2:5960611/3‑148 (MQ=255)
      aTCGCGGCGATATCGAGCCGAAAGTGGTGGCCGAGGCCATCGCCAAGTA‑CGGTATCGATCCGGAGAAGCGCAACCCGCTGGATTGCTAATTCGCTCCATA‑CTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCCACGggcg                                                                                                                                                 >  1:9929377/1‑150 (MQ=255)
       tCGCGGCGATATCGAGCCGAAAGTGGTGGCCGAGGCCATCGCCAAGTA‑CGGTATCGATCCGGAGAAGCGCAACCCGCTGGATTGCTAATTCGCTCCATA‑CTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCCCCCACcggcgg                                                                                                                                                >  1:1292532/1‑150 (MQ=255)
       tCGCGGCGATATCGAGCCGAAAGTGGTGGCCGAGGCCATCGCCAAGTA‑CGGTATCGATCCGGAGAAGCGCAACCCGCTGGATTGCTAAGTCGCTCCATA‑CTCCCCTCGGCAGCGAGGGGTTTCGAGAACGCCGGCGCCGCCAACGggcgg                                                                                                                                                <  2:2830990/150‑1 (MQ=255)
         gcggcgATATCGAGCCGAAAGTGGTGGCCGAGGCCATCGCCAAGTA‑CGGTATCGATCCGGAGAAGCGCAACCCGCTGGATTGCTAATTCGCTCCATA‑CTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCAACGGgcggcg                                                                                                                                              >  2:11162288/1‑150 (MQ=255)
           ggcgATATCGAGCCGAAAGTGGTGGCCGAGGCCATCGCCAAGTA‑CGGTATCGATCCGGAGAAGCGCAACCCGCTGGATTGCTAATTCGCTCCATA‑CTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCAACGGGCGGCGcc                                                                                                                                            <  1:6625902/150‑1 (MQ=255)
            gctatatCGAGCCGAATGTGGGGGCCGAGGCCATCGCCAAGTA‑CTGTATCTATCCGGATAAGCGCACCCCGCTGGATTGCTAATTCGCTCCATA‑CTCCCCTCGGCAGCGTGGGGTTTCGAGCACGCCGGCGCCGCCAACGGGCGGCGCCg                                                                                                                                           <  1:3829386/147‑1 (MQ=255)
             cgATATCGAGCCGAAAGTGGTGGCCGAGGCCATCGCCAAGTA‑CGGTATCGATCCGGAGAAGCGCAACCCGCTGGTTTGCTAATTCGCTCCATA‑CTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCAACGGGCGGCGCCgg                                                                                                                                          <  1:6869177/150‑1 (MQ=255)
             cgATATCGAGCCGAAAGTGGTGGCCGAGGCCATCGCCAAGTA‑CGGTATCGATCCGGAGAAGCGCAACCCGCTGGATTGCTAATTCGCTCCATA‑CTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCAACGGGCGGCGCCgg                                                                                                                                          >  2:7392494/1‑150 (MQ=255)
               atatCGAGCCGAAAGTGGTGGCCGAGGCCATCGCCAAGTA‑CGGTCTCGATCCGGAGAAGCGCAACCCGCTGGATTGCTAATTCGCTCCATA‑CTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCAACGGGCGGCGCCGGCt                                                                                                                                        >  2:9746135/1‑150 (MQ=255)
                tatCGAGCCGAAAGTGGTGGCCGAGGCCATCGCCAAGTA‑CGGTATCGATCCGGAGAAGCGCAACCCGCTGGATTGCTAATTCGCTCCATA‑CTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCAACGGGCGGCGCCGGCTc                                                                                                                                       >  1:3400307/1‑150 (MQ=255)
                  tCGAGCCGAAAGTGGTGGCCGAGGCCATCGCCAAGTA‑CGGAATAGATCCGGAGAAGAGCAACCCGCTGGATTGCTAATTCGCACCATA‑CTCCCATCGTCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCAAAGTGCGGCGCCGGCTaac                                                                                                                                     >  2:2475834/1‑150 (MQ=255)
                        cGAAAGTGGTGGCCGAGGCCATCGCCAAGTA‑CGGTATCGATCCGGAGAAGCGCAACCCGCTGGATTGCTAATTCGCTCCATA‑CTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCCACGGGCGGCGCCGGCTCACAACGaa                                                                                                                               >  2:2335269/1‑150 (MQ=255)
                        cGAAAGTGGTGGCCGAGGCCATCGCCAAGTA‑CGGTATCGATCCGGAGAAGCGCAACCCGCTGGATTGCTAATTCGCTCCATA‑CTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCAACGGGCGGCGCCGGCTCACAACGaa                                                                                                                               >  2:1517143/1‑150 (MQ=255)
                         gAAAGTGGTGGCCGAGGCCATCGCCAAGTA‑CGGTATCGATCCGGAGAAGCGCAACCCGCTGGATTGCTAATTCGCTCCATA‑CTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCCACGGGCGGCGCCGGCTCACAACGAAc                                                                                                                              >  2:10697349/1‑150 (MQ=255)
                           atgtggtgGCCGAGGCCAGCGCAAATTC‑GGGAATCGATCGGTACAAGCGAAACCGGCTGTTTTGAATATTCGCTCATTC‑CTCCCCGCGGCAGCGTGGGTTTTCGAGCCGGCGGGCGCCGCCACCGGGGGGCGCCGGCTCACAACGAACCa                                                                                                                            <  2:6492816/148‑1 (MQ=255)
                            tgggggggCGGAGGCCACCGCCACGTA‑CGGTTTCTATCGGGAGACGCCCAACCCGGTGGTTTGATATTTCGCGCCTCA‑CGCCCCCGGGCGGCGGGGGGTTTGGACCACGCCGGCGCCGCCACGGGGGGGCCCCGGCTCACAACGAACCaa                                                                                                                           <  2:6649544/144‑1 (MQ=255)
                             gtggtgGCCGAGGCCATCGCCAAGTA‑CGGTATCGATCCGGAGAAGCGCAACCCGCTGGATTGCTAATTCGCTCCATA‑CTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCAACGGGCGGCGCCGGCTCACAACGAACCAAg                                                                                                                          >  2:8364000/1‑150 (MQ=255)
                               ggtggCCGAGCCAATCG‑CAACTACCGTTATCTATCGGGAGACGCTCAACCGGCTGTTTTTTTATTTCGCT‑CATACCTCCCCTGGGCAGCGTGGGTTTTGGAGCAGGCCGGCGCCGCCACGGGGGGGCGCCGGCTCACAACGAACCAAGGa                                                                                                                        <  2:2122701/150‑1 (MQ=255)
                                gtggCCTCGGACATCGCAATGTA‑CGGTATGGAGCCGCTGAAGCTAAACCGGGTTGTTTGCTATTTCCATACTTC‑CTCCCCGCGGCTTCGTGGGTTTTCGCGCACGCGGGCGCGGCAACCGGGGGGCGCCGGCTCACAACGAACCAAGGAc                                                                                                                       <  2:4112799/150‑1 (MQ=255)
                                gtgcccgtgcccATCGCAATTTA‑CGTTATAGACCCGGAGAAGCCCAACCGGCTGGTTTGCTAACTCGCACCACA‑CCCCCCTCGGCAGGGGGGGGTATGGTGCACGGGGGCGCGGCCACGGGGCGGCGCCGGCTCACAACGAACCAAGGAc                                                                                                                       <  2:4107639/150‑1 (MQ=255)
                                       aGGCCATCGCCAAGTA‑CGGTATCGATCCGGAGAAGCGCAACCCGCTGGATTGCTAATTCGCTCCATA‑CTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCAACGGGCGGCGCCGGCTCACAACGAACCAAGGACCAAAAGt                                                                                                                >  2:6439070/1‑150 (MQ=255)
                                       aGGCCATCGCCAAGTA‑CGGTATCGATCCGGAGAAGCGCAACCCGCTGGATTGCTAATTCGCTCCATA‑CTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCAACGGGCGGCGCCCGCTCACAACGAACCAAGGACCAAAAGt                                                                                                                >  2:87849/1‑150 (MQ=255)
                                        ggCCATCGCCAAGTA‑CGGTATCGATCCGGAGAAGCGCAACCCGCTGGATTGCTAATTCGCTCCATA‑CTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCAACGGGCGGCGCCGGCTCACACAGAACCCAGGACCGAAATTc                                                                                                               >  2:4738364/1‑150 (MQ=255)
                                         gCCATCGCCAAGTA‑CGGTATCGATCCGGAGAAGCGCAACCCGCTGGATTGCTAATTCGCTCCATA‑CTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCAACGGGCGGCGCCGGCTGGCAACGAACCAAGGACCAAAAGtat                                                                                                              >  1:4896336/1‑148 (MQ=255)
                                          ccATCGCCAAGTA‑CGGTATCGATCCGGAGAAGCGCAACCCGCTGGATTGCTAATTCGCTCCATA‑CTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCAACGGGCGGCGCCCGCTCACCACGAACCAAGGACCAAAAGTCtt                                                                                                             >  2:958782/1‑150 (MQ=255)
                                            aTCGCCATGTC‑GGGTTTCCATCCGTCCACGCCCACCCCGTTGTTTTGATAATACTCTCAATA‑CACCCCTCGCCCGCGTGGGTTTTCGACCACGCCGCCGCCGCAACGGGGGGGCGCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTg                                                                                                           <  2:4288368/150‑1 (MQ=255)
                                                  acgTA‑GGGTATCGCCCCGGATACGCGCAACCCGCGGGATTGCTATTTCGCTCCATC‑CCCCCCTCGGCCGGGTGGGGTTTCTCGCCCGCCGGCGCCGCCACCGGGGGGCGCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTgc                                                                                                     <  2:7039384/148‑1 (MQ=255)
                                                    gTA‑CGGTATCGATCCGGAGAAGCGCAACCCGCTGGATTGCTAATTCGCTCCATA‑CTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCCACGGGCGGCGCCGGCGCACAACGAACCAAGGTCCAAAAGTATGTGTTTTgcgc                                                                                                   >  2:2944903/1‑150 (MQ=255)
                                                        cgttATCGATCCGGAGAAGCCCAACCCGGTGGTTTGCTAATCCGCCCCAAC‑CCCCCCTCGCCCGCGTGGGGTTTCGCCCCCGCCGGCGCCCCCAAGGGGCGGCCCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGcgcc                                                                                                <  2:5606466/147‑1 (MQ=255)
                                                         ggTATCGATCCGGAGAAGCGCAACCCGCTGGATTGCTAATTCGCTCCATA‑CTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCCCCCACCGGCGGCGCCCGCTCACAACCAAACAAAGACCAAAAATGTTTTTTTTTGGcgccc                                                                                               >  1:167179/1‑149 (MQ=255)
                                                          gTATCGATCCGGAGAAGCGCAACCCGCTGGATTGCTAATTCGCTCCATA‑CTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCAACGGGCGGCGCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGcgccgc                                                                                              >  2:10880803/1‑150 (MQ=255)
                                                             tCGATCCGGAGAAGCGCAACCCGCTGGATTGCTAATTCGCTCCATA‑CTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCCCCAACCGGCGGCGCCCGCTCACAACGAAACAAGGTACAAAAGTATTTTTTTTGGGCGCCGccca                                                                                           >  1:897637/1‑150 (MQ=255)
                                                              cGATCCGGAGAAGCGCAACCCGCTGGATTGCTAATTCGCTCCATA‑CTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCCACCGGCGGCGCCGGCGCACAACGAACCAAGGACCCAAAATATTTGTTTTTCGCGCCGcccac                                                                                          >  1:1501907/1‑149 (MQ=255)
                                                              cGAGCCGGATACGCGCACCCGGCGGTATTCATAATTCGCTCCTTC‑CACCCCGCGGCCGGGTGGGGTTTCGACCCCCCCGGCGCCGCCACCGGGCGGCGCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAg                                                                                          <  2:3193100/150‑1 (MQ=255)
                                                                aTCCGGAGAAGCGCAACCCGCTGGATTGCTAATTCGCTCCATA‑CTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCAACCGGCGGCGCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAACg                                                                                        >  1:10107493/1‑150 (MQ=255)
                                                                 tCCGGAGAAGCGCAACCCGCTGGATTGCTAATTCGCTCCATA‑CTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCAACGGGCGGCGCCGGCTCACAACGAAACAAGGACCAAAATTCGTTTTTTTTCGCGCCGCACAGCgg                                                                                       >  1:10156727/1‑150 (MQ=255)
                                                                  ccgtataaGCCCACCCCGCTGTTTGTCTAAGTCGCTCAATA‑CCCCCCTCGGCTGCGTGGGTTTTCGCGCACGCCGCCGCCGCCACCGGGCGGCGCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCggt                                                                                      <  2:450182/144‑1 (MQ=255)
                                                                    ggAGACGCGCAACCCGCTGGATTGCTAATTCGCTCCATA‑CTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCAACGGGCGGCGCCGGCTCACCACGAACCAAGGACCAAAAGTCTTTGTTTTGCGGGCCGCCCAGCggtgg                                                                                    >  1:11068127/1‑150 (MQ=255)
                                                                    ggAGAAGCGCAACCCGCTGGATTGCTAATTCGCTCCATA‑CTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCAACCGGCGGCGCCGGCTCCCAACGAACCAAGGACCAAAAGTCTTTGTTTTGAGCGCCGCACAGCggggg                                                                                    >  2:6157332/1‑150 (MQ=255)
                                                                       cacccgcAACCCGCTGTTTTGATATTTCGCACAATA‑CTCCCCCCGGCAGCGTGGGGTTTCTAGCCCCCCGGCGCCCCCACGGGGCGGCCCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGtggtgg                                                                                 <  1:4370534/146‑1 (MQ=255)
                                                                        acgcgcAACCCGCTGGTTTGCTAATTCGCTCCATC‑CTCCCCTCGCCTGCGTGGGGTTTCGAGCACGCCGGCGCCGCCAACGGGCGGCGCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGc                                                                                <  2:8209656/148‑1 (MQ=255)
                                                                        aaGCGCAACCCGCTGGATTGCTAATTCGCTCCATA‑CTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCCACGGGCGGCGGCGGCTCACAACGAACCAAGGACCAAAAGTGTTTGTTTTGGGCGCCGCACAGCGGTGGTGGc                                                                                >  1:5362555/1‑150 (MQ=255)
                                                                         aGCGCAACCCGCTGGATTGCTAATTCGCTCCATA‑CTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGACGCCAACGGGCGGCGCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCa                                                                               >  2:7246321/1‑150 (MQ=255)
                                                                             caccccGCTGTATTTCTATTTGGCCCAATC‑CCCCCCTCGGCAGGGTGGGTTTTCGAGCCCGCCGGCGCCGCCACCGGGGGGCCCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGTa                                                                           <  1:782615/147‑1 (MQ=255)
                                                                              aaCCCGCTGGATTGCTAATTCGCTCCATA‑CTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCCAAGGGCGGCGCCGGGGGACAACGAACCAAGGGCCAAAATTGTTTGTTTTGCGCGCCGCACCGCGGTGGTGGCACGGaa                                                                          >  1:6227409/1‑150 (MQ=255)
                                                                                        tttGCAAATTCCCCCCAAA‑CACCCCGCGGCTGCGTGGGTTTTCTAGCCCGCCGGCGCCCCCACGGGGGGGCCCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGTAAAAGGAGAGGa                                                                <  2:5034568/149‑1 (MQ=255)
                                                                                        agtgatAATGCGCACCATA‑CTCCCCTCGGAAGCGAGGGGTTTCGAGCACGCCGGCGCCCCCACCGGGCGGCGCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGTAAAAGGAGAGGa                                                                <  2:7927232/145‑1 (MQ=255)
                                                                                         tttcttaTTCGCACAATA‑CCCCCCTCGTCCGCTTGGGGTTTCTACCACGCGGGCGCCGAATAGGGGCGGCCCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGTAAAAGGAGAGGaa                                                               <  1:585364/144‑1 (MQ=255)
                                                                                         ttGCTAATTCGCTCCATA‑CTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCAACGGGCGGCGCCGGCTCACAACGAAACAAGGACCAAAATTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGGAAAAGGAGAGGaa                                                               >  2:8490718/1‑150 (MQ=255)
                                                                                           gatAATACGCTCAATC‑CCCCCCTCGGCCGCGCGGGTTTTCGACCCCGCCGGCGCCGCCACCGGGCGGCGCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGTAAAAGGAGAGGAAtg                                                             <  1:10685355/148‑1 (MQ=255)
                                                                                            cTAATTCGCTCCATA‑CTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCAACGGGCGGCGCCGGCTGACAACGAACCCAGGTCCAAAAGTCTTTGTTTTGGGCGCCGCACAGCGGGGGTGGCGCGGAAAAGGAGGGGAAtgt                                                            >  1:7442002/1‑150 (MQ=255)
                                                                                             gaaTGCGCTCCATC‑CGCCCCTCGGCAGCGAGGGGTTGCGAGCCCGCCGGCGCCCCCAACGGGCGGCGCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGTAAAAGGAGAGGAAtgtg                                                           <  2:8624191/149‑1 (MQ=255)
                                                                                              acttCGCCCCATC‑CTCCCCTCGCCCGCGCGGGGTTACCACCACCCCGGCGCCGCACACGGGCGGCGCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGTAAAAGGAGAGGAATGTGa                                                          <  2:7853248/148‑1 (MQ=255)
                                                                                              aaTTCGCTCCATA‑CTCCCCTGGGCTGCTTGGGGTTGCGCGCACGCGGGCGCCCCCAAGGGGCGGCCCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGTAAAAGGAGAGGAATGTGa                                                          <  2:9318155/150‑1 (MQ=255)
                                                                                              aaTGCGCTCCATA‑CCCCCCGCGGCTGCGTGGGTTTTCGCCCCCGCCGGCGCCCCCACCGGGCGGCCCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGTAAAAGGAGAGGAATGTGa                                                          <  1:8067184/150‑1 (MQ=255)
                                                                                               aTTCGCTCCAAA‑CCCCCCGCGGCAGGGGGGGTTTGCGCCCCCCCCGGCGCCCCCACGGGGCGGCCCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGTAAAAGGAGAGGAATGTGAg                                                         <  1:4437753/150‑1 (MQ=255)
                                                                                                ttCGCTCCATC‑CGCCCCTCGCCCGGGTGGGTTTTCTAGCACGCCGGCGCCGCCAAGGGGCGGCGCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGTAAAAGGAGAGGAATGTGAGc                                                        <  2:9266419/150‑1 (MQ=255)
                                                                                                tacGCTCCATA‑CTCCCCTCGCCCGCGTGGGGTTTCGAGCACGCCGGCGCCGCCACCGGGCGGCGCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGTAAAAGGAGAGGAATGTGAGc                                                        <  1:8231039/148‑1 (MQ=255)
                                                                                                      ccATC‑CTCCCCGCGGCCGGGTGGGGTCGCGACCCCGCGGGCGCCCCCCCGGGGCGGCGCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGTAAAAGGAGAGGAATGTGAGCGAACTc                                                  <  1:9352948/150‑1 (MQ=255)
                                                                                                        aTA‑CTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCAACGGGCGGCGCCGGCGCACAACGAACCACGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGTAAAAGGAGAGGAATGTGGGCGAACTCAt                                                >  2:8837686/1‑150 (MQ=255)
                                                                                                         atccTCCCCTCGGCAGCGTGGGTTTTCGAGCACGCCGGCGCCGCCAACGGGCGGCGCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGTAAAAGGAGAGGAATGTGAGCGAACTCAt                                                <  2:4379968/147‑1 (MQ=255)
                                                                                                          cccTCCCCTCGGCAGCGCGGGTTTTCGACCACCCCGGCGCCGCCACCGGGCGGCCCCGGCTCTCAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGTAAAAGGAGAGGAATGTGAGCGAACTCAtt                                               <  2:3925767/148‑1 (MQ=255)
                                                                                                          a‑CTCCCCACGGCAGCGTGGGGTTTCGAGCACGCCGGCGCCGCCAACGGGCGGCGCCGGCTCACATCGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGTAAAAGGAGAGGAATGTGAGCGAACTCATTc                                              <  2:3439092/150‑1 (MQ=255)
                                                                                                            cTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCAACGGGCGGCGCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGTAAAAGGAGAGGAATGTGAGCGAACTCATTcg                                             >  2:1727753/1‑150 (MQ=255)
                                                                                                             ccccaTCGGCTGCGTGGGTTTTCCCCCCCGCCGGCCCCCCCCCGGGGCGGCCCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGTAAAAGGAGAGGAATGTGAGCGAACTCATTcgc                                            <  1:1349023/149‑1 (MQ=255)
                                                                                                              ccccTCGGCTGGGTGGGTTTACGCCCCCGCCGGCGCCGCCACGGGGCGGCCCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGTAAAAGGAGAGGAATGTGAGCGAACTCATTcgcg                                           <  1:7662758/150‑1 (MQ=255)
                                                                                                               cccTGGGCCGTGTGGGTTTTCGTCCACGCGGGCGCCGCACAGGGGCGGCCCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGTAAAAGGAGAGGAATGTGAGCGAACTCATTCGCGt                                          <  1:8118022/150‑1 (MQ=255)
                                                                                                                ccgcGGCAGGGTGGGGTTTCGCGCACGCCGGCGCCGCCAAGGGGCGGCGCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGTAAAAGGAGAGGAATGTGAGCGAACTCATTCGCGTa                                         <  2:1831064/147‑1 (MQ=255)
                                                                                                                ccTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCCACGGGCGGCGCCGGCTCACAACGAACCAAGGACCCAAAGTCTTTGTTTTGCGCGCCGCACCGCGGGGGGGGGTCGGCCAAGGAGGGGAATGTGAGCGAACTCATTAGCGTa                                         >  2:10347898/1‑150 (MQ=255)
                                                                                                                 cTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCAACGGGCGGCGCCGGCGCACAACGAACCAAGGACCCAAAGTCTTTTTTTTTCGCGCCGCACCGCGGGGGGGGGACGTAAAAGGAGAGGAATGTGAGCGAACTCATTAGCGTaa                                        >  1:10860391/1‑149 (MQ=255)
                                                                                                                   cGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCAACGGGCGGCGCCGGCTCACAACGAACCAATGAACCAAAATATTTGTTTTGCGCGGCGCACCGCGGTGGTGGGACGTTAAATGGGAGGAATGTGAGCGAACGAATTCGCGTAccc                                      >  1:4517950/1‑150 (MQ=255)
                                                                                                                           aGGGGTTTCGAGCACGCCGGCGCCGCCCACGGGCGGCGCCGGCTAACCACGAACCAAGGTCCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACCGAAAAGGCGAGGACTGTGAGCGAACTCATTCGCGTACCCGACATCgg                              >  2:6042908/1‑150 (MQ=255)
                                                                                                                                tttCGCGCCCGCCGGCGCCGCCACGGGGGGGCCCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGTAAAAGGAGAGGAATGTGAGCGAACTCATTCGCGTACCCGACATCGGCAACg                         <  1:4126699/150‑1 (MQ=255)
                                                                                                                                  tCGAGCCCGCGGGCGCCGCCACGGGGGGGCCCCGGCTCACAACGAATCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGTAAAAGGAGAGGAATGTGAGCGAACTCATTCGCGTACCCGACATCGGCAACGGt                       <  1:8038403/150‑1 (MQ=255)
                                                                                                                                   ctcccccGCCGGCCCCCCCCCGGGGGGGCCCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGTAAAAGGAGAGGAATGTGAGCGAACTCATTCGCGTACCCGACATCGGCAACGGTg                      <  1:4617757/144‑1 (MQ=255)
                                                                                                                                   cccccACGCCCGCGCCCCCCCCGGGCGGCCCCGGCTCACAACCAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGTAAAAGGAGAGGAATGTGAGCGAACTCATTCGCGTACCCGACATCGGCAACGGTg                      <  1:2973034/146‑1 (MQ=255)
                                                                                                                                     cgCACGCCGCCGCCCCCACCGGCCGGCGCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGTAAAAGGAGAGGAATGTGAGCGAACTCATTCGCGTACCCGACATCGGCAACGGTGAg                    <  2:5477721/149‑1 (MQ=255)
                                                                                                                                       cACGCCGGCGCCGCCACCGGGCGGCGCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGTAAAAGGAGAGGAATGTGAGCGAACTCATTCGCGTACCCGACATCGGCAACGGTGAggg                  <  2:5530537/150‑1 (MQ=255)
                                                                                                                                        aCGCCGGCGCCGCCAACGGGCGGCGCCGGCGCACAACGAACCAAGGACCCAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGGACGGAAAAGGAGTGGAATGTGAGCGAACTCATTCGCGTACCCCCCAAACGCAACGGGGAgggg                 >  2:6864501/1‑149 (MQ=255)
                                                                                                                                          gCCGGCGCCGCCACCGGGCGGCGCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGTAAAAGGAGAGGAATGTGAGCGAACTCATTCGCGTACCCGACATCGGCAACGGTGAGGGTGa               <  1:7118507/150‑1 (MQ=255)
                                                                                                                                           cgggCGCCGCCACGGGGGGCCGCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGTAAAAGGAGAGGAATGTGAGCGAACTCATTCGCGTACCCGACATCGGCAACGGTGAGGGTGaa              <  1:859778/148‑1 (MQ=255)
                                                                                                                                           ccGGCGCCCCCACGGGGCGGCGCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGTAAAAGGAGAGGAATGTGAGCGAACTCATTCGCGTACCCGACATCGGCAACGGTGAGGGTGaa              <  1:2385034/150‑1 (MQ=255)
                                                                                                                                            cGGCGCCCCCAACGGGCGGCGCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGTAAAAGGAGAGGAATGTGAGCGAACTCATTCGCGTACCCGACATCGGCAACGGTGAGGGTGAAg             <  2:3781828/150‑1 (MQ=255)
                                                                                                                                              gcgcCGCCAACGGGCGGCGCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGTAAAAGGAGAGGAATGTGAGCGAACTCATTCGCGTACCCGACATCGGCAACGGTGAGGGTGAAGTc           >  2:5335700/1‑150 (MQ=255)
                                                                                                                                              gcgcCGCCAACGGGCGGCGCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGTAAAAGGAGAGGAATGTGAGCGAACTCATTCGCGTACCCGACATCGGCAACGGTGAGGGTGAAGTc           >  2:2217547/1‑150 (MQ=255)
                                                                                                                                                gccgccAACGGGCGGCGCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGTAAAAGGAGAGGAATGTGAGCGAACTCATTCGCGTACCCGACATCGGCAACGGTGAGGGTGAAGTCAt         >  1:1755229/1‑150 (MQ=255)
                                                                                                                                                ccccccACCGGGCGGCCCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGTAAAAGGAGAGGAATGTGAGCGAACTCATTCGCGTACCCGACATCGGCAACGGTGAGGGTGAAGTCAt         <  1:2826740/146‑1 (MQ=255)
                                                                                                                                                   gccAACGGGCGGCGCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGTAAAAGGAGAGGAATGTGAGCGAACTCATTCGCGTACCCGACATCGGCAACGGTGAGGGTGAAGTCATCGa      >  1:6900873/1‑150 (MQ=255)
                                                                                                                                                   gccAACGGGCGGCGCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGTAAAAGGAGAGGAATGTGAGCGAACTCATTCGCGTACCCGACATCGGCAACGGTGAGGGTGAAGTCATCGa      >  1:10037021/1‑150 (MQ=255)
                                                                                                                                                    ccAACGGGCGGCGCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGTAAAAGGAGAGGAATGTGAGCGAACTCATTCGCGTACCCGACATCGGCAACGGTGAGGGTGAAGTCATCGAg     >  2:10510036/1‑150 (MQ=255)
                                                                                                                                                    ccAACGGGCGGCGCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGTAAAAGGAGAGGAATGTGAGCGAACTCATTCGCGTACCCGACATCGGCAACGGTGAGGGTGAAGTCATCGAg     >  2:6015721/1‑150 (MQ=255)
                                                                                                                                                       aCGGGCGGCGCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGTAAAAGGAGAGGAATGTGAGCGAACTCATTCGCGTACCCGACATCGGCAACGGTGAGGGTGAAGTCATCGAgctg  >  1:2590664/1‑150 (MQ=255)
                                                                                                                                                       aCGGGCGGCCCCGGCACACCACGAACCAAGCACCAAACCTCTTTGGTTTGCGCGCCGCACAGCGGTGGTGGCACGTAAAAGGAGAGGAATGAGAGCGAACTCATTCGCGTACCCGACATCGGCACCGGTGAGGGTGAAGTCATCGAgctg  <  2:9003288/150‑1 (MQ=255)
                                                                                                                                                       |                                                                                                                                                     
TGGCCGATCGCGGCGATATCGAGCCGAAAGTGGTGGCCGAGGCCATCGCCAAGTA‑CGGTATCGATCCGGAGAAGCGCAACCCGCTGGATTGCTAATTCGCTCCATA‑CTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCAACGGGCGGCGCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGTAAAAGGAGAGGAATGTGAGCGAACTCATTCGCGTACCCGACATCGGCAACGGTGAGGGTGAAGTCATCGAGCTG  >  NC_008463/5910107‑5910405

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑