Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 2,955,468 A→G L277L (TTG→CTG)  PA14_RS13700 ← PepSY domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084632,955,4680AG99.7% 1429.2 / NA 369L277L (TTG→CTG) PA14_RS13700PepSY domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (198/170);  minor base T (1/0);  total (199/170)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 369 total aligned reads displayed.

CGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAAGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCAGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAAGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTGCGGCGCGGGGCACGTCGGCGCGTGCGATGACCGGAGGGATGCCGTCGTTGGTCA  >  NC_008463/2955321‑2955614
                                                                                                                                                   |                                                                                                                                                  
cGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGTCGTTGCCGGGCAGGa                                                                                                                                                  >  2:2595692/1‑150 (MQ=255)
cGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGa                                                                                                                                                  <  1:57264/150‑1 (MQ=255)
 ggTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGaa                                                                                                                                                 >  2:3620340/1‑150 (MQ=255)
   tGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAaca                                                                                                                                               <  2:6140750/150‑1 (MQ=255)
    gACGAACTGCCGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAacac                                                                                                                                              <  2:166199/150‑1 (MQ=255)
    gACGAACTGCAGGGGGGTACGGTCGCTGCGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAacac                                                                                                                                              <  2:9378378/150‑1 (MQ=255)
    gACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAacac                                                                                                                                              <  1:3046133/150‑1 (MQ=255)
        aaCTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACcgcg                                                                                                                                          >  2:8866343/1‑150 (MQ=255)
        aaCTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACcgcg                                                                                                                                          >  2:7195386/1‑150 (MQ=255)
        aaCTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACcgca                                                                                                                                          >  1:9822927/1‑149 (MQ=255)
         aCTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCgcgc                                                                                                                                         >  1:10740462/1‑150 (MQ=255)
         aCTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCgcgc                                                                                                                                         <  2:4494611/150‑1 (MQ=255)
            gCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTgg                                                                                                                                      >  1:1820577/1‑150 (MQ=255)
            gCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTgg                                                                                                                                      >  2:5692303/1‑150 (MQ=255)
             cAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGc                                                                                                                                     <  2:9055018/150‑1 (MQ=255)
             cAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGc                                                                                                                                     <  1:2767583/150‑1 (MQ=255)
              aGGGGGGTACGGTCGCATAGCAGGCGGGGGGTTTCGATCTTGCCGGTATAAGGGTTCTGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCCCGAACATCGGGCTGGGGGCGTGGCCGGGCCGGAAGAGCGCGCGGGcc                                                                                                                                    >  2:5858179/1‑150 (MQ=255)
               ggggggTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCt                                                                                                                                   >  1:117414/1‑150 (MQ=255)
                 ggggTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTcc                                                                                                                                 <  1:9024026/150‑1 (MQ=255)
                    gTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAgg                                                                                                                              <  1:4795550/150‑1 (MQ=255)
                     tACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGc                                                                                                                             >  1:3753022/1‑150 (MQ=255)
                        ggTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTgg                                                                                                                          <  1:6672594/150‑1 (MQ=255)
                        ggTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTgg                                                                                                                          <  2:4726529/150‑1 (MQ=255)
                        ggTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTgg                                                                                                                          >  1:632288/1‑150 (MQ=255)
                         gTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTggg                                                                                                                         >  1:358196/1‑150 (MQ=255)
                          tCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTgggg                                                                                                                        >  2:7250684/1‑150 (MQ=255)
                           cGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGa                                                                                                                       <  1:9275824/150‑1 (MQ=255)
                            gCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGAt                                                                                                                      >  2:3349364/1‑150 (MQ=255)
                            gCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGAt                                                                                                                      <  2:8138626/150‑1 (MQ=255)
                             cTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATa                                                                                                                     >  2:7501146/1‑150 (MQ=255)
                             cTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATa                                                                                                                     <  1:4984100/150‑1 (MQ=255)
                             cTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATa                                                                                                                     <  1:1691951/150‑1 (MQ=255)
                             cTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATa                                                                                                                     <  2:5211067/150‑1 (MQ=255)
                                  caggCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGccc                                                                                                                <  1:1492546/147‑1 (MQ=255)
                                  caggCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGccc                                                                                                                >  2:6026015/4‑150 (MQ=255)
                                    ggCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCgg                                                                                                              >  2:10949234/2‑150 (MQ=255)
                                    ggCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCgg                                                                                                              >  1:3428110/2‑150 (MQ=255)
                                    ggCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCgg                                                                                                              >  1:2900370/2‑150 (MQ=255)
                                      cGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTg                                                                                                            <  2:8407852/150‑1 (MQ=255)
                                       gggTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGa                                                                                                           <  1:5380776/150‑1 (MQ=255)
                                        ggtggtTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGAc                                                                                                          >  1:8043043/1‑150 (MQ=255)
                                           ggtTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAg                                                                                                       >  2:1176020/1‑150 (MQ=255)
                                            gtTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGc                                                                                                      <  2:4588502/150‑1 (MQ=255)
                                             tttCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGcc                                                                                                     <  1:4250560/150‑1 (MQ=255)
                                                  aTCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCAcc                                                                                                >  1:10206053/1‑150 (MQ=255)
                                                       gCCGGTAGAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTc                                                                                           <  1:8427743/150‑1 (MQ=255)
                                                        ccGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCg                                                                                          <  2:5451164/150‑1 (MQ=255)
                                                            tataAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCa                                                                                      >  2:6624611/1‑150 (MQ=255)
                                                              taAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGc                                                                                    >  2:3823998/1‑150 (MQ=255)
                                                                 gggTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCg                                                                                 >  1:4832088/1‑150 (MQ=255)
                                                                  ggTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGa                                                                                <  1:6497554/150‑1 (MQ=255)
                                                                  ggTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGa                                                                                >  1:10592914/1‑150 (MQ=255)
                                                                   gTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGAt                                                                               <  2:2834822/150‑1 (MQ=255)
                                                                    ttCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCACCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGACCAGCTCGATg                                                                              >  2:2938000/1‑150 (MQ=255)
                                                                      cAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTa                                                                            <  1:5704639/150‑1 (MQ=255)
                                                                          gcgGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGa                                                                        >  1:9937504/1‑150 (MQ=255)
                                                                          gcgGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGa                                                                        >  1:7057757/1‑150 (MQ=255)
                                                                           cgGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGaa                                                                       <  1:135114/150‑1 (MQ=255)
                                                                            ggCCGACTGGAACATGAGCGGATCCCAGCCGCGTCCGGCCACGAACATCGGGCGGTCGGCGTTGCCGGGCAGGCACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCCCCGCCTCGTCCAGCTCGATGTATGGAAc                                                                      >  2:4015398/1‑150 (MQ=255)
                                                                                 aCTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCt                                                                 <  2:8765161/150‑1 (MQ=255)
                                                                                 aCTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTGGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCt                                                                 <  1:215236/150‑1 (MQ=255)
                                                                                  cTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTg                                                                <  2:6504684/150‑1 (MQ=255)
                                                                                    ggAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGAc                                                              >  1:10974144/1‑150 (MQ=255)
                                                                                    ggAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGAc                                                              <  2:9443495/150‑1 (MQ=255)
                                                                                    ggAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGAc                                                              >  1:5593658/1‑150 (MQ=255)
                                                                                     gAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGAcc                                                             >  1:3006634/1‑150 (MQ=255)
                                                                                     gAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGAcc                                                             <  1:9695217/150‑1 (MQ=255)
                                                                                      aaCATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGAGGTCTGGACCCTTCTGACCg                                                            >  2:952349/1‑150 (MQ=255)
                                                                                       aCATGAGCGGCTCCCAGCCGCGTCCGGCCACGAACCTCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCgg                                                           <  2:240548/150‑1 (MQ=255)
                                                                                       aCATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCCGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCgg                                                           >  2:9847672/1‑150 (MQ=255)
                                                                                        cATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCggg                                                          >  1:4372524/1‑150 (MQ=255)
                                                                                        cATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCGGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTAGGGAACCTTCTGACCggg                                                          >  2:10638459/1‑150 (MQ=255)
                                                                                          tGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTg                                                        >  2:11151078/1‑150 (MQ=255)
                                                                                          tGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTg                                                        >  2:5510843/1‑150 (MQ=255)
                                                                                            aGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTgcg                                                      <  1:7811249/150‑1 (MQ=255)
                                                                                             gCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTgcgg                                                     >  1:2292716/1‑150 (MQ=255)
                                                                                             gCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTgcgg                                                     <  1:3075580/150‑1 (MQ=255)
                                                                                             gCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGAGACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGACCCTTCTGACCGGGTgcgg                                                     >  2:6767029/1‑150 (MQ=255)
                                                                                                    ccAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTGCGGCGCgggg                                              <  2:7781446/150‑1 (MQ=255)
                                                                                                      aGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTGCGGCGCGGGGCa                                            >  2:3108176/1‑150 (MQ=255)
                                                                                                        ccGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTGCGGCGCGGGGCACg                                          <  1:8184839/150‑1 (MQ=255)
                                                                                                        ccGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTGCGGCGCGGGGCACg                                          <  2:2759891/150‑1 (MQ=255)
                                                                                                            gTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGCGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTGCGGCGCGGGGCACGTCgg                                      <  2:10580005/150‑1 (MQ=255)
                                                                                                            gTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTGCGGCGCGGGGCACGTCgg                                      >  2:4242775/1‑150 (MQ=255)
                                                                                                               cggCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTGCGGCGCGGGGCACGTCGgcgc                                   >  2:10411332/3‑150 (MQ=255)
                                                                                                               cggCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTGCGGCGCGGGGCACGTCGgcgc                                   >  2:3537851/3‑150 (MQ=255)
                                                                                                                 gCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGCTACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTGCGGCGCGGGGCACGTCGGCGCGt                                 >  2:6269764/1‑150 (MQ=255)
                                                                                                                 gCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTGCGGCGCGGGGCACGTCGGCGCGt                                 <  2:8965031/150‑1 (MQ=255)
                                                                                                                      gAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTGCGGCGCGGGGCACGTCGGCGCGTGCGAt                            <  1:2610923/150‑1 (MQ=255)
                                                                                                                       aaCATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCACGATGTATGGAACCTTCTGACCGGGTGCGGCGCGGGGCACGTCGGCGCGTGCGATg                           >  2:1129433/1‑150 (MQ=255)
                                                                                                                        aCATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTGCGGCGCGGGGCACGTCGGCGCGTGCGATGa                          <  1:6191088/150‑1 (MQ=255)
                                                                                                                           tCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTGCGGCGCGGGGCACGTCGGCGCGTGCGATGACCg                       <  2:10164256/150‑1 (MQ=255)
                                                                                                                              ggCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTGCGGCGCGGGGCACGTCGGCGCGTGCGATGACCGGAg                    >  1:7438796/1‑150 (MQ=255)
                                                                                                                               gCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTGCGGCGCGGGGCACGTCGGCGCGTGCGATGACCGGAgg                   >  2:2098713/1‑150 (MQ=255)
                                                                                                                                      gCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTGCGGCGCGGGGCACGTCGGCGCGTGCGATGACCGGAGGGATGCcg            <  1:10384756/150‑1 (MQ=255)
                                                                                                                                        gTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTGCGGCGCGGGGCACGTCGGCGCGTGCGATGACCGGAGGGATGCcgtc          <  1:10101413/150‑1 (MQ=255)
                                                                                                                                         ttGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTGCGGCGCGGGGCACGTCGGCGCGTGCGATGACCGGAGGGATGCcgtcg         >  2:741483/1‑150 (MQ=255)
                                                                                                                                          tGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTGCGGCGCGGGGCACGTCGGCGCGTGCGATGACCGGAGGGATGCcgtcgt        <  2:225972/150‑1 (MQ=255)
                                                                                                                                           gCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTGCGGCGCGGGGCACGTCGGCGCGTGCGATGACCGGAGGGATGCCGTCGtt       >  2:1534796/1‑150 (MQ=255)
                                                                                                                                                gCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTGCGGCGCGGGGCACGTCGGCGCGTGCGATGACCGGAGGGATGCCGTCGTTGGTCa  <  2:1841203/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAAGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCAGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAAGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTGCGGCGCGGGGCACGTCGGCGCGTGCGATGACCGGAGGGATGCCGTCGTTGGTCA  >  NC_008463/2955321‑2955614

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑