Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 2,985,345 A→G K2233E (AAA→GAA)  pvdD → pyoverdine non‑ribosomal peptide synthetase PvdD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084632,985,3450AG100.0% 1569.4 / NA 392K2233E (AAA→GAA) pvdDpyoverdine non‑ribosomal peptide synthetase PvdD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (201/191);  total (201/191)

Only 100 of 395 total aligned reads displayed.

TGTTACAAGACCCTCGCCATGGCGCTGCGGGATCGTCATCCGGTCAAGGGTGTTGTCTGCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCAAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCGGCTGGATCGATGCGCCGGCACCGGTCAGGGTCGAAGCGTTCTGGAAC  >  NC_008463/2985198‑2985491
                                                                                                                                                   |                                                                                                                                                  
  ttACAAGACCCTCGCCATGGCGCTGCGGGATCGTCATCCGGTCAAGGGTGTTGTCTGCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAgg                                                                                                                                                <  1:1806935/150‑1 (MQ=255)
   tACAAGACCCTCGCCATGGCGCTGCGGGATCGTCATCCGGTCAAGGGTGTTGTCTGCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAggg                                                                                                                                               >  1:2675628/1‑150 (MQ=255)
     cAAGACCCTCGCCATGGCGCTGCGGGATCGTCATCCGGTCAAGGGTGTTGTCTGCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGt                                                                                                                                             <  2:311145/150‑1 (MQ=255)
     cAAGACCCTCGCCATGGCGCTGCGGGATCGTCATCCGGTCAAGGGTGTTGTCTGCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGt                                                                                                                                             <  2:833149/150‑1 (MQ=255)
     cAAGACCCTCGCCATGGCGCTGCGGGATCGTCATCCGGTCAAGGGTGTTGTCTGCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGt                                                                                                                                             >  1:9882569/1‑150 (MQ=255)
      aaGACCCTCGCCATGGCGCTGCGGGATCGTCATCCGGTCAAGGGTGTTGTCTGCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGtt                                                                                                                                            <  1:963251/150‑1 (MQ=255)
      aaGACCCTCGCCATGGCGCTGCGGGATCGTCATCCGGTCAAGGGTGTTGTCTGCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGtt                                                                                                                                            <  1:8600447/150‑1 (MQ=255)
        gACCCTCGCCATGGCGCTGCGGGATCGTCATCCGGTCAAGGGTGTTGTCTGCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGtttt                                                                                                                                          <  1:574969/150‑1 (MQ=255)
         aCCCTCGCCATGGCGCTGCGGGATCGTCATCCGGTCAAGGGTGTTGTCTGCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTc                                                                                                                                         >  1:2237216/1‑150 (MQ=255)
          cccTCGCCATGGCGCTGCGGGATCGTCATCCGGTCAAGGGTGTTGTCTGCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCa                                                                                                                                        <  1:11067342/150‑1 (MQ=255)
           ccTCGCCATGGCGCTGCGGGATCGTCATCCGGTCAAGGGTGTTGTCTGCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCaa                                                                                                                                       <  1:8315405/150‑1 (MQ=255)
           ccTCGCCATGGCGCTGCGGGATCGACATCCGGTCAAGGGTGTTGTCTGCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCaa                                                                                                                                       <  1:7424434/150‑1 (MQ=255)
            cTCGCCATGGCGCTGCGGGATCGTCATCCGGTCAAGGGTGTTGTCTGCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAAc                                                                                                                                      <  1:8930080/150‑1 (MQ=255)
             tCGCCATGGCGCTGCGGGATCGTCATCCGGTCAAGGGTGTTGTCTGCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAAcc                                                                                                                                     >  1:8834025/1‑150 (MQ=255)
             tCGCCATGGCGCTGCGGGATCGTCATCCGGTCAAGGGTGTTGTCTGCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAAcc                                                                                                                                     >  2:1184809/1‑150 (MQ=255)
              cGCCATGGCGCTGCGGGATCGTCATCCGGTCAAGGGTGTTGTCTGCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCt                                                                                                                                    <  1:5245241/150‑1 (MQ=255)
               gCCATGGCGCTGCGGGATCGTCATCCGGTCAAGGGTGTTGTCTGCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTg                                                                                                                                   >  2:251138/1‑150 (MQ=255)
                ccATGGCGCTGCGGGATCGTCATCCGGTCAAGGGTGTTGTCTGCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTgg                                                                                                                                  >  1:10996400/1‑150 (MQ=255)
                 cATGGCGCTGCGGGATCGTCATCCGGTCAAGGGTGTTGTCTGCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGc                                                                                                                                 >  2:9600105/1‑150 (MQ=255)
                 cATGGCGCTGCGGGATCGTCATCCGGTCAAGGGTGTTGTCTGCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGc                                                                                                                                 <  1:3016608/150‑1 (MQ=255)
                   tGGCGCTGCGGGATCGTCATCCGGTCAAGGGTGTTGTCTGCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCgg                                                                                                                               >  1:2085044/1‑150 (MQ=255)
                     gcgcTGCGGGATCGTCATCCGGTCAAGGGTGTTGTCTGCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGa                                                                                                                             <  1:5931891/150‑1 (MQ=255)
                     gcgcTGCGGGATCGTCATCCGGTCAAGGGTGTTGTCTGCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGa                                                                                                                             <  1:2694048/150‑1 (MQ=255)
                     gcgcTGCGGGATCGTCATCCGGTCAAGGGTGTTGTCTGCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGa                                                                                                                             >  1:2908556/1‑150 (MQ=255)
                       gcTGCGGGATCGTCATCCGGTCAAGGGTGTTGTCTGCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATg                                                                                                                           >  1:352698/1‑150 (MQ=255)
                         tGCGGGATCGTCATCCGGTCAAGGGTGTTGTCTGCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGt                                                                                                                         <  2:3241375/150‑1 (MQ=255)
                         tGCGGGATCGTCATCCGGTCAAGGGTGTTGTCTGCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGt                                                                                                                         <  2:2851636/150‑1 (MQ=255)
                         tGCGGGATCGTCATCCGGTCAAGGGTGTTGTCTGCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGt                                                                                                                         <  1:4481073/150‑1 (MQ=255)
                               aTCGTCATCCGGTCAAGGGTGTTGTCTGCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTcg                                                                                                                   >  1:194630/1‑150 (MQ=255)
                                tCGTCATCCGGTCAAGGGTGTTGTCTGCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTcgg                                                                                                                  <  1:4927769/150‑1 (MQ=255)
                                tCGTCATCCGGTCAAGGGTGTTGTCTGCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTcgg                                                                                                                  >  1:5092372/1‑150 (MQ=255)
                                 cGTCATCCGGTCAAGGGTGTTGTCTGCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTcggc                                                                                                                 >  2:7152725/1‑150 (MQ=255)
                                   tCATCCGGTCAAGGGTGTTGTCTGCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTcggcgg                                                                                                               <  1:7472323/150‑1 (MQ=255)
                                    cATCCGGTCAAGGGTGTTGTCTGCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCggcggc                                                                                                              >  1:4342965/1‑150 (MQ=255)
                                     agccGGTCAAGGGTGTTGTCTGCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCa                                                                                                             <  2:217842/148‑1 (MQ=255)
                                     aTCCGGTCAAGGGTGTTGTCTGCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCa                                                                                                             <  1:710781/150‑1 (MQ=255)
                                       ccGGTCAAGGGTGTTGTCTGCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAAc                                                                                                           >  1:4205039/1‑150 (MQ=255)
                                          gTCAAGGGTGTTGTCTGCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTg                                                                                                        <  1:4623143/150‑1 (MQ=255)
                                             aaGGGTGTTGTCTGCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGCGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCg                                                                                                     >  2:4519574/1‑150 (MQ=255)
                                              aGGGTGTTGTCTGCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCga                                                                                                    <  2:5694777/150‑1 (MQ=255)
                                                  tgttgtCTGCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCgatgga                                                                                                <  2:3392462/150‑1 (MQ=255)
                                                        cTGCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTcgc                                                                                          >  1:7210575/1‑150 (MQ=255)
                                                         tGCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTcgcg                                                                                         >  2:9315784/1‑150 (MQ=255)
                                                               gCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCgg                                                                                   <  1:9815390/150‑1 (MQ=255)
                                                                 ccTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCt                                                                                 >  2:9521663/1‑150 (MQ=255)
                                                                 ccTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCt                                                                                 <  1:1588769/150‑1 (MQ=255)
                                                                    gctgGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGa                                                                              <  2:1796868/150‑1 (MQ=255)
                                                                        ggCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGTATACGCCGTGCAATTGCTGCAGGAGCACCCCGATGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGAGGGAGGTCGCGGCCCGGCTGGagcag                                                                          <  2:5418943/150‑1 (MQ=255)
                                                                        ggCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGagcag                                                                          >  2:6488459/1‑150 (MQ=255)
                                                                         gcgcTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGagcagc                                                                         <  2:10319180/150‑1 (MQ=255)
                                                                          cgcTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCg                                                                        <  1:7718834/150‑1 (MQ=255)
                                                                           gcTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGt                                                                       <  2:5849653/150‑1 (MQ=255)
                                                                            cTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTg                                                                      >  2:7905192/1‑150 (MQ=255)
                                                                            cTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTg                                                                      >  1:10276634/1‑150 (MQ=255)
                                                                            cTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTg                                                                      >  2:139417/1‑150 (MQ=255)
                                                                                 cgcgAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGggcgg                                                                 >  1:8467192/1‑150 (MQ=255)
                                                                                   cgAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCa                                                               >  1:10676390/1‑150 (MQ=255)
                                                                                    gAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAg                                                              <  1:1081119/150‑1 (MQ=255)
                                                                                    gAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAg                                                              >  2:8764740/1‑150 (MQ=255)
                                                                                            ggAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGCTGGTCGCTCGGCGGCAACCTGGCGCTGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCttt                                                      <  2:4948725/150‑1 (MQ=255)
                                                                                            ggAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCttt                                                      >  2:11043034/1‑150 (MQ=255)
                                                                                               gTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTtcgt                                                   <  2:2697769/150‑1 (MQ=255)
                                                                                                tGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTtcgtc                                                  >  2:7648039/1‑150 (MQ=255)
                                                                                                   gACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCGGc                                               <  2:4664463/150‑1 (MQ=255)
                                                                                                   gACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCGGc                                               >  2:3925546/1‑150 (MQ=255)
                                                                                                   gACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCGGc                                               <  2:6820195/150‑1 (MQ=255)
                                                                                                      gATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCGGCTgg                                            >  1:5554127/1‑150 (MQ=255)
                                                                                                      gATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCGGCTgg                                            >  2:7451945/1‑150 (MQ=255)
                                                                                                      gATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCGGCTgg                                            <  1:3169201/150‑1 (MQ=255)
                                                                                                        tatGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCGGCTGGAt                                          >  2:5205087/1‑150 (MQ=255)
                                                                                                        tatGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCGGCTGGAt                                          <  2:975354/150‑1 (MQ=255)
                                                                                                              tGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCGGCTGGATCGATgc                                    <  1:9570032/150‑1 (MQ=255)
                                                                                                              tGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCGGCTGGATCGATgc                                    >  2:10450770/1‑150 (MQ=255)
                                                                                                                cGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCGGCTGGATCGATgcgc                                  >  1:2975060/1‑150 (MQ=255)
                                                                                                                cGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCGGCTGGATCGATgcgc                                  <  2:8972060/150‑1 (MQ=255)
                                                                                                                 ggAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCGGCTGGATCGATGCGcc                                 >  1:2439536/1‑150 (MQ=255)
                                                                                                                  gAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCGGCTGGATCGATGCGCCg                                >  2:2873800/1‑150 (MQ=255)
                                                                                                                  gAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCGGCTGGATCGATGCGCCg                                >  2:6327248/1‑150 (MQ=255)
                                                                                                                     tACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCGGCTGGATCGATGCGCCGGCa                             <  1:6120675/150‑1 (MQ=255)
                                                                                                                       cGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCGGCTGGATCGATGCGCCGGCAcc                           >  1:9189827/1‑150 (MQ=255)
                                                                                                                       cGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCGGCTGGATCGATGCGCCGGCAcc                           >  1:6721770/1‑150 (MQ=255)
                                                                                                                        gCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCGGCTGGATCGATGCGCCGGCACCg                          >  2:1990632/1‑150 (MQ=255)
                                                                                                                        gCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCGGCTGGATCGATGCGCCGGCACCg                          >  1:4878661/1‑150 (MQ=255)
                                                                                                                        gCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCGGCTGGATCGATGCGCCGGCACCg                          <  1:3960756/150‑1 (MQ=255)
                                                                                                                         ccGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCGGCTGGATCGATGCGCCGGCACCgg                         >  1:8104432/1‑150 (MQ=255)
                                                                                                                         ccGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTATCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCGGCTGGATCGATGCGCCGGCACCgg                         >  1:1162317/1‑150 (MQ=255)
                                                                                                                           gAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCGGCTGGATCGATGCGCCGGCACCGGTc                       >  1:10105065/1‑150 (MQ=255)
                                                                                                                           gAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCGGCTGGATCGATGCGCCGGCACCGGTc                       >  1:3808379/1‑150 (MQ=255)
                                                                                                                           gAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCGGCTGGATCGATGCGCCGGCACCGGTc                       <  1:2506936/150‑1 (MQ=255)
                                                                                                                               aaTTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCGGCTGGATCGATGCGCCGGCACCGGTCAggg                   >  2:377188/1‑150 (MQ=255)
                                                                                                                                 ttGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCGGCTGGATCGATGCGCCGGCACCGGTCAGGGTc                 <  2:8160414/150‑1 (MQ=255)
                                                                                                                                   gctgcAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCGGCTGGATCGATGCGCCGGCACCGGTCAGGGTCGa               >  2:3612752/1‑150 (MQ=255)
                                                                                                                                      gcAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCGGCTGGATCGATGCGCCGGCACCGGTCAGGGTCGAAGc            >  1:10533904/1‑150 (MQ=255)
                                                                                                                                      gcAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCGGCTGGATCGATGCGCCGGCACCGGTCAGGGTCGAAGc            >  2:4276754/1‑150 (MQ=255)
                                                                                                                                        aGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCGGCTGGATCGATGCGCCGGCACCGGTCAGGGTCGAAGCGt          >  1:6474879/1‑150 (MQ=255)
                                                                                                                                        aGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCGGCTGGATCGATGCGCCGGCACCGGTCAGGGTCGAAGCGt          >  2:5543716/1‑150 (MQ=255)
                                                                                                                                        aGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCGGCTGGATCGATGCGCCGGCACCGGTCAGGGTCGAAGCGt          >  1:10013394/1‑150 (MQ=255)
                                                                                                                                           aGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCGGCTGGATCGATGCGCCGGCACCGGTCAGGGTCGAAGCGTTCt       <  1:3752879/150‑1 (MQ=255)
                                                                                                                                              aCCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCGGCTGGATCGATGCGCCGGCACCGGTCAGGGTCGAGGCGTTCTGGa    >  2:8490108/1‑150 (MQ=255)
                                                                                                                                               ccccGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCGGCTGGATCGATGCGCCGGCACCGGTCAGGGTCGAAGCGTTCTGGaa   >  2:10986597/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TGTTACAAGACCCTCGCCATGGCGCTGCGGGATCGTCATCCGGTCAAGGGTGTTGTCTGCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCAAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCGGCTGGATCGATGCGCCGGCACCGGTCAGGGTCGAAGCGTTCTGGAAC  >  NC_008463/2985198‑2985491

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑