BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_008463	3,387,881	A→C	M47L (ATG→CTG)	cynS →	cyanase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_008463	3,387,881	0	A	C	99.8%	1611.5 / NA	414	M47L (ATG→CTG)	cynS	cyanase
Reads supporting (aligned to +/- strand): ref base A (0/0); major base C (178/235); minor base G (0/1); total (178/236)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 414 total aligned reads displayed.

AGTCATCCCATGCAACACAGCCAAGTGTCCCCCAACGCGCGCCAGCAACTGGCCGAGACCGTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTATCGCCCTGACATTAGTCACCGCCGCCATGTTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCATGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGCGACCCGACCATCTAC  >  NC_008463/3387734‑3388027

 gTCATCCCATGCAACACAGCCAAGTGTCCCCCAACGCGCGCCAGCAACTGGCCGAGACCGTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTgc                                                                                                                                                 <  1:68341/150‑2 (MQ=255)

       ccATGCAACACAGCCAAGTGTCCCCCAACGCGCGCCAGCAACTGGCCGAGACCGTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGcc                                                                                                                                           >  2:2513469/1‑150 (MQ=255)

        cATGCAACACAGCCAAGTGTCCCCCAACGCGCGCCAGCAACTGGCCGAGACCGTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCa                                                                                                                                          <  1:1479417/150‑1 (MQ=255)

         aTGCAACACAGCCACGTGTCCCCCAACGCGCGCCAGCAACTGGCCGAGACCGTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAg                                                                                                                                         >  2:9700447/1‑150 (MQ=255)

           gCAACACAGCCAAGTGTCCCCCAACGCGCGCCAGCAACTGGCCGAGACCGTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCa                                                                                                                                       <  1:6447277/150‑1 (MQ=255)

           gCAACACAGCCAAGTGTCCCCCAACGCGCGCCAGCAACTGGCCGAGACCGTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCa                                                                                                                                       <  1:1699649/150‑1 (MQ=255)

            cAACACAGCCAAGTGTCCCCCCACGCGCGCCAGCAACGGGCCGAGACCGTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCAt                                                                                                                                      <  1:3520684/150‑1 (MQ=255)

            cAACACAGCCAAGTGTCCCCCAACGCGCGCCAGCAACTGGCCGAGACCGTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCAt                                                                                                                                      >  2:8417390/1‑150 (MQ=255)

               cacaGCCAAGTGTCCCCCAACGCGCGCCAGCAACTGGCCGAGACCGTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGcc                                                                                                                                   <  2:8385014/150‑1 (MQ=255)

                acaGCCAAGTGTCCCCCAACGCGCGCCAGCAACTGGCCGAGACCGTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGccc                                                                                                                                  <  1:5135172/150‑1 (MQ=255)

                 caGCCAAGTGTCCCCCAACGCGCGCCAGCAACTGGCCGAGACCGTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCt                                                                                                                                 >  1:5758497/1‑150 (MQ=255)

                 caGCCAAGTGTCCCCCAACGCGCGCCAGCAACTGGCAGAGACCGTGGTCCAGAACCTGGCCCGCCGGTGCCAGTCATGGCAGGATCTGGCCGACGGCACCTGTCTCGCCCTGTCATTCGTCACCGCCGCCCGGCTCGGCCAGCATGCCCt                                                                                                                                 >  1:3881605/1‑150 (MQ=255)

                      aaGTGTCCCCCAACGCGCGCCAGCAACTGGCCGAGACCGTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCag                                                                                                                            <  1:1984397/150‑1 (MQ=255)

                      aaGTGTCCCCCAACGCGCGCCAGCAACTGGCCGAGACCGTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCag                                                                                                                            >  2:10234675/1‑150 (MQ=255)

                      aaGTGTCCCCCAACGCGCGCCAGCAACTGGCCGAGACCGTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCag                                                                                                                            >  1:8988745/1‑150 (MQ=255)

                       aGTGTCCCCCAACGCGCGCCAGCAACTGGCCGAGTCCGTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCTGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCaga                                                                                                                           <  1:10127607/150‑1 (MQ=255)

                        gtgtCCCCCAACGCGCGCCAGCAACTGGCCGAGACCGTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCagag                                                                                                                          >  1:2432283/1‑150 (MQ=255)

                           tCCCCCAACGCGCGCCAGCAACTGGCCGAGACCGTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAggcg                                                                                                                       <  1:2109660/150‑1 (MQ=255)

                            cccccAACGCGCGCCAGCAACTGGCCGAGACCGTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAggcgg                                                                                                                      >  1:10008705/1‑150 (MQ=255)

                             ccccAACGCGCGCCAGCAACTGGCCGAGACCGTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAggcggc                                                                                                                     >  2:2858603/1‑150 (MQ=255)

                               ccAACGCGCGCCAGCAACTGGCCGAGACCGTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAggcggcgg                                                                                                                   <  1:7146201/150‑1 (MQ=255)

                               ccAACGCGCGCCAGCAACTGGCCGAGACCGTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAggcggcgg                                                                                                                   <  2:3040420/150‑1 (MQ=255)

                                 aaCGCGCGCCAGCAACTGGCCGAGACCGTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCTTGCCCTGCCAGTGgcggcggcg                                                                                                                 >  2:10118854/1‑150 (MQ=255)

                                 aaCGCGCGCCAGCAACGGGCCGAGACCGTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGgcggcggcg                                                                                                                 <  2:9902134/150‑1 (MQ=255)

                                   cgcgcgCCAGCAACTGGCCGAGACCGTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGgcgcg                                                                                                               >  2:5738930/1‑150 (MQ=255)

                                       cgcCAGCAACTGGCCGAGACCGTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAg                                                                                                           <  1:9249174/150‑1 (MQ=255)

                                        gcCAGCAACTGGCCGAGACCGTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAgg                                                                                                          <  2:7140697/150‑1 (MQ=255)

                                         ccAGCAACTGGCCGAGACCGTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGt                                                                                                         <  2:4524456/150‑1 (MQ=255)

                                            gCAACTGGCCGAGACCGTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTcgc                                                                                                      >  2:2140412/1‑150 (MQ=255)

                                            gCAACTGGCCGAGACCGTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTcgc                                                                                                      >  1:555466/1‑150 (MQ=255)

                                              aaCTGGCCGAGACCGTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCCGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTcgccg                                                                                                    >  1:9468437/1‑150 (MQ=255)

                                                   gCCGAGACCGTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCaa                                                                                               >  2:133226/1‑150 (MQ=255)

                                                   gCCGAGACCGTGGTCCTGAACAAGGCCCGCCAGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCac                                                                                               >  2:3489161/1‑149 (MQ=255)

                                                    ccGAGACCGTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAAc                                                                                              >  2:88888/1‑150 (MQ=255)

                                                      gagaCCGTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTc                                                                                            <  1:8554520/150‑1 (MQ=255)

                                                      gagaCCGAGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCGGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTc                                                                                            <  2:7365273/150‑1 (MQ=255)

                                                       agaCCGTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCg                                                                                           >  2:3346099/1‑150 (MQ=255)

                                                        gaCCGTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCgg                                                                                          >  2:11098745/1‑150 (MQ=255)

                                                                    gAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGgacgacga                                                                              <  2:4385812/150‑1 (MQ=255)

                                                                      aCAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCACCTCGGCCTGGccgtcgtcg                                                                            >  2:9778041/1‑150 (MQ=255)

                                                                         aGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCgg                                                                         >  1:8037679/1‑150 (MQ=255)

                                                                          ggCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGt                                                                        <  1:9694317/150‑1 (MQ=255)

                                                                           gCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTg                                                                       >  1:8349992/1‑150 (MQ=255)

                                                                            cccGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGc                                                                      <  2:1232618/150‑1 (MQ=255)

                                                                             ccGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCt                                                                     <  1:10778696/150‑1 (MQ=255)

                                                                                  tGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCctgc                                                                >  2:4043617/1‑150 (MQ=255)

                                                                                  cgggCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCATCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTCCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCctgc                                                                <  2:5774752/149‑1 (MQ=255)

                                                                                     gCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCtgctgc                                                             <  2:9406249/150‑1 (MQ=255)

                                                                                       cTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTTCaa                                                           <  2:5621758/150‑1 (MQ=255)

                                                                                         gTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAg                                                         <  1:4293347/150‑1 (MQ=255)

                                                                                          tCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGc                                                        <  2:6235953/150‑1 (MQ=255)

                                                                                           ccTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCa                                                       <  2:9221863/150‑1 (MQ=255)

                                                                                            cTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCAt                                                      <  1:11128891/150‑1 (MQ=255)

                                                                                               gCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATccc                                                   <  1:2580808/150‑1 (MQ=255)

                                                                                                 aGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGc                                                 <  1:3138578/150‑1 (MQ=255)

                                                                                                   gATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTg                                               <  2:8482311/150‑1 (MQ=255)

                                                                                                    aTCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAACGCATCCCGCTgc                                              <  2:3772296/150‑1 (MQ=255)

                                                                                                     tCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTgcg                                             >  2:8682337/1‑150 (MQ=255)

                                                                                                      cTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTgcgc                                            <  2:6637468/150‑1 (MQ=255)

                                                                                                        ggCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCgg                                          >  1:6081815/1‑150 (MQ=255)

                                                                                                         gCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGgc                                         <  1:9563091/150‑1 (MQ=255)

                                                                                                          ccGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGgca                                        <  2:4220385/150‑1 (MQ=255)

                                                                                                           cGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGgcag                                       <  1:7531753/150‑1 (MQ=255)

                                                                                                           cGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGgcag                                       <  1:3659946/150‑1 (MQ=255)

                                                                                                            gACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGgcagc                                      <  1:3364017/150‑1 (MQ=255)

                                                                                                             aCGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGgcagca                                     <  1:1131873/150‑1 (MQ=255)

                                                                                                             aCGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGgcagca                                     >  1:8937522/1‑150 (MQ=255)

                                                                                                             aCGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGgcagca                                     <  2:4178616/150‑1 (MQ=255)

                                                                                                               ggCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCAtt                                   <  2:494520/150‑1 (MQ=255)

                                                                                                                gCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTc                                  <  2:7389179/150‑1 (MQ=255)

                                                                                                                 cACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTcc                                 >  2:7040123/1‑150 (MQ=255)

                                                                                                                  aCCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTccc                                >  2:5906170/1‑150 (MQ=255)

                                                                                                                    cGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCcgg                              >  1:10575048/1‑150 (MQ=255)

                                                                                                                    cGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCAGTCCcgg                              >  2:6787473/1‑150 (MQ=255)

                                                                                                                     ggTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCcggc                             >  1:6475070/1‑150 (MQ=255)

                                                                                                                      gtctCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCcggcg                            >  2:9607475/4‑150 (MQ=255)

                                                                                                                       tctCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCcggcgg                           <  1:4381164/148‑1 (MQ=255)

                                                                                                                            cccTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTc                      >  1:8823070/1‑150 (MQ=255)

                                                                                                                            cccTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTc                      >  1:8454501/1‑150 (MQ=255)

                                                                                                                            cccTGACCTTCGTCACCGCCGCCCTGCACGGCCAGCACGCCCAGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTc                      <  2:10859659/150‑1 (MQ=255)

                                                                                                                              cggACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCCGAGGCGGCGGCGCGCCAGGTCGCCGCCCAACTCGGCCTGGACGACGTCGCGGTGCTCCTGCTGCAAAGCATCGCGCTGCGCGGCAGCATTCGCGGCGGCATTccc                    >  2:9498583/3‑150 (MQ=255)

                                                                                                                               tGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCa                   <  1:1267858/150‑1 (MQ=255)

                                                                                                                                gACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCACCTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAg                  <  2:10578310/150‑1 (MQ=255)

                                                                                                                                gACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAg                  <  2:5226935/150‑1 (MQ=255)

                                                                                                                                gACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAg                  <  2:10430434/150‑1 (MQ=255)

                                                                                                                                 accttcgTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGc                 <  1:4730131/144‑1 (MQ=255)

                                                                                                                                 accttcgTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGc                 <  1:6224914/144‑1 (MQ=255)

                                                                                                                                  ccttcgTCACCGCCGCCCTGCTCGGCCAGCCTGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGcg                <  2:8195499/145‑1 (MQ=255)

                                                                                                                                  ccttcgTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGcg                <  1:6653936/145‑1 (MQ=255)

                                                                                                                                    ttcgTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCAGCCCGCTGCGCGGCAGCAGTCCCGGCGGCATTCCCAGcgac              <  2:7803804/147‑1 (MQ=255)

                                                                                                                                     tcgTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGcgacc             <  2:5092267/148‑1 (MQ=255)

                                                                                                                                     tcgTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGcgacc             <  1:9856626/148‑1 (MQ=255)

                                                                                                                                     tcgTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGcgacc             >  2:1658733/3‑150 (MQ=255)

                                                                                                                                       gTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGcgacccg           >  1:2883057/1‑150 (MQ=255)

                                                                                                                                        tCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGcgacccga          <  1:4130853/150‑1 (MQ=255)

                                                                                                                                        tCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGcgacccga          <  1:10366972/150‑1 (MQ=255)

                                                                                                                                         cACCGCCGCCCTGCTCGGCCAGCAGGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCGGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGcgacccgac         <  1:4020538/150‑1 (MQ=255)

                                                                                                                                            cgccgcCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGCGACCCGACCAt      >  1:8501454/1‑150 (MQ=255)

                                                                                                                                               cgccCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGCGACCCGACCATCTa   <  1:766195/150‑1 (MQ=255)

                                                                                                                                                gccCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGCGACCCGACCATCTAc  <  1:5376251/150‑1 (MQ=255)

AGTCATCCCATGCAACACAGCCAAGTGTCCCCCAACGCGCGCCAGCAACTGGCCGAGACCGTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTATCGCCCTGACATTAGTCACCGCCGCCATGTTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCATGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGCGACCCGACCATCTAC  >  NC_008463/3387734‑3388027

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`