Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 3,387,941 A→C M67L (ATG→CTG)  cynS → cyanase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084633,387,9410AC99.8% 1692.0 / ‑4.7 433M67L (ATG→CTG) cynScyanase
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base C (147/285);  total (148/285)
Fisher's exact test for biased strand distribution p-value = 3.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

Only 100 of 433 total aligned reads displayed.

GTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTATCGCCCTGACATTAGTCACCGCCGCCATGTTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCATGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGCGACCCGACCATCTACCGCTTCTACGAGATGCTGCAAGTCTACGGCAGCACCCTGAAGGCACTGGTCCACGAACAG  >  NC_008463/3387794‑3388087
                                                                                                                                                   |                                                                                                                                                  
gTGGTCCTGAACCAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCCTGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTg                                                                                                                                                  <  1:10980405/150‑1 (MQ=255)
gTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTg                                                                                                                                                  >  1:6278639/1‑150 (MQ=255)
 tGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTgg                                                                                                                                                 >  2:7476163/1‑150 (MQ=255)
     ccTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCCCCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGgacga                                                                                                                                             <  2:4193689/150‑1 (MQ=255)
        gAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGgacgacga                                                                                                                                          <  2:4385812/150‑1 (MQ=255)
        gAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGgacgacga                                                                                                                                          <  1:9340846/150‑1 (MQ=255)
           cAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGAcgc                                                                                                                                       >  2:7017277/1‑150 (MQ=255)
           cAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGAcgc                                                                                                                                       <  1:279000/150‑1 (MQ=255)
               gCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTg                                                                                                                                   <  1:2044145/150‑1 (MQ=255)
               gCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTg                                                                                                                                   >  1:8349992/1‑150 (MQ=255)
                 ccGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCt                                                                                                                                 <  1:10778696/150‑1 (MQ=255)
                   gCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCAGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTcc                                                                                                                               >  2:6386842/1‑150 (MQ=255)
                     cTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCctg                                                                                                                             >  2:10150739/1‑150 (MQ=255)
                      tGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCctgc                                                                                                                            >  2:4043657/1‑150 (MQ=255)
                      cgggCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCATCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTCCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCctgc                                                                                                                            <  2:5774752/149‑1 (MQ=255)
                         gCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCtgctgc                                                                                                                         <  1:5803596/150‑1 (MQ=255)
                           cTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTTCaa                                                                                                                       <  2:5621758/150‑1 (MQ=255)
                             gTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAg                                                                                                                     <  1:4293347/150‑1 (MQ=255)
                             gTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAg                                                                                                                     <  1:10012056/150‑1 (MQ=255)
                              tCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGc                                                                                                                    >  1:3053151/1‑150 (MQ=255)
                               ccTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCa                                                                                                                   <  2:9220746/150‑1 (MQ=255)
                                cTGGCAGGATCGGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCAt                                                                                                                  <  2:5105657/150‑1 (MQ=255)
                                 tGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATc                                                                                                                 <  1:8537628/150‑1 (MQ=255)
                                     aGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGc                                                                                                             <  2:3367085/150‑1 (MQ=255)
                                     aGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGc                                                                                                             <  2:3051589/150‑1 (MQ=255)
                                       gATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTg                                                                                                           <  2:8482311/150‑1 (MQ=255)
                                       gATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTg                                                                                                           >  1:9796426/1‑150 (MQ=255)
                                        gtCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCCGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCACAGCATGCCGGTgc                                                                                                          >  1:9601512/2‑150 (MQ=255)
                                        aTCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTgc                                                                                                          <  2:3772274/150‑1 (MQ=255)
                                           tGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGcgcg                                                                                                       <  1:6666133/150‑1 (MQ=255)
                                            ggCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCgg                                                                                                      <  1:4073745/150‑1 (MQ=255)
                                            ggCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCgg                                                                                                      >  1:6081815/1‑150 (MQ=255)
                                               cGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGgcag                                                                                                   <  1:7531753/150‑1 (MQ=255)
                                               cGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGgcag                                                                                                   <  1:3659946/150‑1 (MQ=255)
                                                 aCGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGgcagca                                                                                                 <  1:1118952/150‑1 (MQ=255)
                                                 aCGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGgcagca                                                                                                 <  2:7686418/150‑1 (MQ=255)
                                                    gCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTc                                                                                              <  2:9753906/150‑1 (MQ=255)
                                                     cACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTcc                                                                                             <  1:10628314/150‑1 (MQ=255)
                                                      aCCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTccc                                                                                            >  2:5906170/1‑150 (MQ=255)
                                                        cGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCcgg                                                                                          <  1:5193461/150‑1 (MQ=255)
                                                         ggTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCcggc                                                                                         >  1:6475070/1‑150 (MQ=255)
                                                                cccTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTc                                                                                  >  1:8454941/1‑150 (MQ=255)
                                                                cccTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTc                                                                                  >  1:9051543/1‑150 (MQ=255)
                                                                 ccTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCGGCGCGGCAGCAGTCCCGGCGGCATTcc                                                                                 <  2:9578533/150‑1 (MQ=255)
                                                                  cTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTccc                                                                                >  2:5385359/1‑150 (MQ=255)
                                                                    gACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCACCTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAg                                                                              <  2:10578310/150‑1 (MQ=255)
                                                                    gACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAg                                                                              <  2:10430434/150‑1 (MQ=255)
                                                                      ccttcgTCACCGCCGCCCTGCTCGGCCAGCCTGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGcg                                                                            <  2:8195499/145‑1 (MQ=255)
                                                                             cACCGCCGCCCTGCTCGGCCAGCAGGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCGGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGcgacccgac                                                                     <  1:4020538/150‑1 (MQ=255)
                                                                                cgccgcCCTGCTCGGCCAGCATGCCCTGCCAGAGTCGGCGGCGCGCAAGGTCGACGCCCAACTCCGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGCGACCCGACCAt                                                                  <  2:4936702/150‑1 (MQ=255)
                                                                                cgccgcCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGCGACCCGACCAt                                                                  <  2:9411610/150‑1 (MQ=255)
                                                                                   cgccCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGCGACCCGACCATCTa                                                               <  1:766195/150‑1 (MQ=255)
                                                                                    gccCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGCGACCCGACCATCTAc                                                              <  2:2578787/150‑1 (MQ=255)
                                                                                     ccctgctCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGCGACCCGACCATCTAcc                                                             <  1:5088534/144‑1 (MQ=255)
                                                                                     ccctgctCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGCGACCCGACCATCTAcc                                                             >  1:5463289/7‑150 (MQ=255)
                                                                                      cctgctCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGCGACCCGACCATCTACCg                                                            <  2:6681953/145‑1 (MQ=255)
                                                                                        tgctCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGCGACCCGACCATCTACCGCt                                                          >  2:1492073/4‑150 (MQ=255)
                                                                                         gctCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGCGACCCGACCATCTACCGCtt                                                         <  1:1177251/148‑1 (MQ=255)
                                                                                           tCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGCGACCCGACCATCTACCGCTTCt                                                       <  1:8897323/150‑1 (MQ=255)
                                                                                            cGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGCGACCCGACCATCTACCGCTTCTa                                                      <  2:4559315/150‑1 (MQ=255)
                                                                                            cGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGCGACCCGACCATCTACCGCTGCTa                                                      >  2:1242634/1‑150 (MQ=255)
                                                                                             ggCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGCGACCCGACCATCTACCGCTTCTAc                                                     >  2:6071218/1‑150 (MQ=255)
                                                                                             ggCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGCGACCCGACCATCTACCGCTTCTAc                                                     >  2:7868563/1‑150 (MQ=255)
                                                                                               ccAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGCGACCCGACCATCTACCGCTTCTACga                                                   <  1:7074225/150‑1 (MQ=255)
                                                                                                   cATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGCGACCCGACCATCTACCGCTTCTACGAGAtg                                               >  1:9488155/1‑150 (MQ=255)
                                                                                                    aTGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGCGACCCGACCATCTACCGCTTCTACGAGAtgc                                              <  1:10275691/150‑1 (MQ=255)
                                                                                                      gCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGCGACCCGACCATCTACCGCTTCTACGAGAtgctg                                            <  2:11032787/150‑1 (MQ=255)
                                                                                                        ccTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGCGACCCGACCATCTACCGCTTCTACGAGATGCTGCa                                          >  2:1074045/1‑150 (MQ=255)
                                                                                                           gCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGCGACCCGACCATCTACCGCTTCTACGAGATGCTGCAAGt                                       <  2:9867678/150‑1 (MQ=255)
                                                                                                            ccAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTTGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCAGTCCCGGCGGCATTCCCAGCGACCCGACCATCTACCGCTTCTACGAGATGCTGCAAGTc                                      >  1:137821/1‑150 (MQ=255)
                                                                                                            ccAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGCGACCCGACCATCTACCGCTTCTACGAGATGCTGCAAGTc                                      <  2:2064062/150‑1 (MQ=255)
                                                                                                            ccAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGCGACCCGACCATCTACCGCTTCTACGAGATGCTGCAAGTc                                      >  1:1888916/1‑150 (MQ=255)
                                                                                                            ccAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGCGACCCGACCATCTACCGCTTCTACGAGATGCTGCAAGTc                                      <  1:8950062/150‑1 (MQ=255)
                                                                                                              agagGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGCGACCCGACCATCTACCGCTTCTACGAGATGCTGCAAGTCTa                                    <  1:4936558/150‑1 (MQ=255)
                                                                                                               gagGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGCGACCCGACCATCTACCGCTTCTACGAGATGCTGCACGTCTAc                                   >  1:6435697/1‑150 (MQ=255)
                                                                                                               gagGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGCGACCCGACCATCTACCGCTTCTACGAGATGCTGCAAGTCTAc                                   <  1:10477265/150‑1 (MQ=255)
                                                                                                                 ggcggcggcGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGCGACCCGACCATCTACCGCTTCTACGAGATGCTGCTAGTCTACgg                                 >  1:245801/1‑150 (MQ=255)
                                                                                                                 ggcggcggcGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGCGACCCGACCATCTACCGCTTCTACGAGATGCTGCAAGTCTACgg                                 <  1:5890937/150‑1 (MQ=255)
                                                                                                                    ggcggcgCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGCGACCCGACCATCTACCGCTTCTACGAGATGCTGCAAGTCTACGgcag                              <  2:8620895/150‑1 (MQ=255)
                                                                                                                     gcggcgCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGCGACCCGACCATCTACCGCTTCTACGAGATGCTGCAAGTCTACGgcagc                             <  1:1647159/150‑1 (MQ=255)
                                                                                                                         cgcgcAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGCGACCCGACCATCTACCGCTTCTACGAGATGCTGCAAGTCTACGGCAGCAccc                         <  1:2331083/150‑1 (MQ=255)
                                                                                                                          gcgcAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGCGACCCGACCATCTACCGCTTCTACGAGATGCTGCAAGTCTACGGCAGCACCCt                        <  2:7188177/150‑1 (MQ=255)
                                                                                                                           cgcAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGCGACCCGACCATCTACCGCTTCTACGAGATGCTGCAAGTCTACGGCAGCACCCTg                       >  2:5163458/1‑150 (MQ=255)
                                                                                                                           cgcAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGCGACCCGACCATCTACCGCTTCTACGAGATGCTGCAAGTCTACGGCAGCACCCTg                       >  2:6202459/1‑150 (MQ=255)
                                                                                                                            gcAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGCGACCCGACCATCTACCGCTTCTACGAGATGCTGCAAGTCTACGGCAGCACCCTGa                      <  1:8619470/150‑1 (MQ=255)
                                                                                                                            gcAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGCGACCCGACCATCTACCGCTTCTACGAGATGCTGCAAGTCTACGGCAGCACCCTGa                      <  1:4143594/150‑1 (MQ=255)
                                                                                                                            gcAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGCGACCCGACCATCTACCGCTTCTACGAGATGCTGCAAGTCTACGGCAGCACCCTGa                      <  1:9937188/150‑1 (MQ=255)
                                                                                                                             cAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGCGACCCGACCATCTACCGCTTCTACGAGCTGCTGCAAGTCTACGGCAGCACCCTGaa                     >  2:9086808/1‑150 (MQ=255)
                                                                                                                               aGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGCGACCCGACCATCTACCGCTTCTACGAGATGCTGCAAGTCTACGGCAGCACCCTGAAgg                   >  1:6803664/1‑150 (MQ=255)
                                                                                                                               aGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGCGACCCGACCATCTACCGCTTCTACGAGATGCTGCAAGTCTACGGCAGCACCCTGAAgg                   <  2:4322184/150‑1 (MQ=255)
                                                                                                                               aGGGCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCAGTCCCAGCGACCCGACCATCTACCGCTTCTACGAGATGCTGCAAGTCTACGTCAGCACCCTGAAgg                   >  2:10883343/1‑150 (MQ=255)
                                                                                                                                 gTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGCGACCCGACCATCTACCGCTTCTACGAGATGCTGCAAGTCTACGGCAGCACCCTGAAGGcc                 >  2:11155322/1‑149 (MQ=255)
                                                                                                                                      cgccCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGCGACCCGACCATCTACCGCTTCTACGAGATGCTGCAAGTCTACGGCAGCACCCTGAAGGCACTGGt            <  1:4553413/150‑1 (MQ=255)
                                                                                                                                      cgccCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGCGACCCGACCATCTACCGCTTCTACGAGATGCTGCAAGTCTACGGCAGCACCCTGAAGGCACTGGt            >  1:3350591/1‑150 (MQ=255)
                                                                                                                                       gccCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGCGACCCGACCATCTACCGCTTCTACGAGATGCTGCAAGTCTACGGCAGCACCCTGAAGGCACTGGTc           <  1:8066929/150‑1 (MQ=255)
                                                                                                                                       gccCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGCGACCCGACCATCTACCGCTTCTACGAGATGCTGCAAGTCTACGGCAGCACCCTGAAGGCACTGGTc           >  2:7063477/1‑150 (MQ=255)
                                                                                                                                        cccAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGCGACCCGACCATCTACCGCTTCTACGAGATGCTGCAAGTCTACGGCAGCACCCTGAAGGCACTGGTcc          >  2:1746044/1‑150 (MQ=255)
                                                                                                                                         ccAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGCGACCCGACCATCTACCGCTTCTACGAGATGCTGCAAGTCTACGGCAGCACCCTGAAGGCACTGGTCCa         <  2:217712/150‑1 (MQ=255)
                                                                                                                                          cAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGCGACCCGACCATCTACCGCTTCTACGAGATGCTGCAAGTCTACGGCAGCACCCTGAAGGCACTGGTCCAc        <  2:3673094/150‑1 (MQ=255)
                                                                                                                                              tCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGCGACCCGACCATCTACCGCTTCTACGAGATGCTGCAAGTCTACGGCAGCACCCTGAAGGCACTGGTCCACGAAc    <  1:1271336/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTATCGCCCTGACATTAGTCACCGCCGCCATGTTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCATGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGCGACCCGACCATCTACCGCTTCTACGAGATGCTGCAAGTCTACGGCAGCACCCTGAAGGCACTGGTCCACGAACAG  >  NC_008463/3387794‑3388087

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑