Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 3,443,292 C→G P459A (CCC→GCC)  PA14_RS15680 → GntP family permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084633,443,2920CG99.5% 1623.8 / NA 411P459A (CCC→GCC) PA14_RS15680GntP family permease
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base G (206/203);  minor base T (1/1);  total (207/204)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 416 total aligned reads displayed.

GGCGGGATGGATACCCTGCCGCACAACGGCGCGGTGATCACCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACCCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTGGGCATCGCCCTGAGCCTGGCCGAGGCCGGCGCCGACCTG  >  NC_008463/3443145‑3443438
                                                                                                                                                   |                                                                                                                                                  
ggcggGATGGATACCCTGCCGCACAACGGCGCGGTGATCACCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTAcgcc                                                                                                                                                  <  1:42460/150‑1 (MQ=255)
ggcggGATGGATACCCTGCCGCACAACGGCGCGGTGATCACCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTAcgcc                                                                                                                                                  >  2:6242614/1‑150 (MQ=255)
 gcggGATGGATACCCTGCCGCACAACGGCGCGGTGATCACCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCa                                                                                                                                                 >  2:3458372/1‑150 (MQ=255)
  cggGATGGATACCCTGCCGCACAACGGCGCGGTGATCACCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCAc                                                                                                                                                >  1:1911688/1‑150 (MQ=255)
  cggGATGGATACCCTGCCGCACAACGGCGCGGTGATCACCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCAc                                                                                                                                                <  1:2403146/150‑1 (MQ=255)
   gggATGGATACCCTGCCGCACAACGGCGCGGTGATCACCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCAcc                                                                                                                                               >  1:254919/1‑150 (MQ=255)
       tggatACCCTGCCGCACAACGGCGCGGTGATCACCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGcc                                                                                                                                           >  1:10530987/1‑150 (MQ=255)
          atACCCTGCCGCACAACGGCGCGGTGATCACCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTgg                                                                                                                                        >  1:9777825/1‑150 (MQ=255)
            aCCCTGCCGCACAACGGCGCGGTGATCACCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTTCAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTc                                                                                                                                      <  2:7294950/150‑1 (MQ=255)
              ccTGCCGCACAACGGCGCGGTGATCACCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTg                                                                                                                                    <  1:926711/150‑1 (MQ=255)
                 gCCGCACCACGGCTCGGTGATCACCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTATGATc                                                                                                                                 <  2:10977087/150‑1 (MQ=255)
                   cGCACAACGGCGCGGTGATCACCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCg                                                                                                                               >  2:7505285/1‑150 (MQ=255)
                     cacaACGGCGCGGTGATCACCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGcc                                                                                                                             <  2:8430843/150‑1 (MQ=255)
                      acaACGGCGCGGTGATCACCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGccc                                                                                                                            <  1:3583136/150‑1 (MQ=255)
                       caACGGCGCGGTGATCACCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCt                                                                                                                           <  2:6121731/150‑1 (MQ=255)
                          cGGCGCGGTGATCACCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCtttt                                                                                                                        <  1:438124/150‑1 (MQ=255)
                           ggCGCGGTGATCACCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCttttt                                                                                                                       <  2:4412414/150‑1 (MQ=255)
                           ggCGCGGTGATCACCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCttttt                                                                                                                       <  2:1021566/150‑1 (MQ=255)
                           ggCGCGGTGATCACCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCttttt                                                                                                                       <  1:10373507/150‑1 (MQ=255)
                            gcgcGGTGATCACCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCtttttt                                                                                                                      <  1:10265585/150‑1 (MQ=255)
                            gcgcGGTGATCACCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCtttttt                                                                                                                      <  1:5655388/150‑1 (MQ=255)
                              gcgGTGATCACCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCa                                                                                                                    <  2:3755952/150‑1 (MQ=255)
                                   gATCACCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGTTCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTg                                                                                                               >  2:9816245/1‑150 (MQ=255)
                                   gATCACCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGGCTGATCCGCCCTTTTTTCATCCTg                                                                                                               >  2:5380116/1‑150 (MQ=255)
                                    aTCACCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGc                                                                                                              <  2:1704926/150‑1 (MQ=255)
                                      cACCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCgg                                                                                                            <  1:8123228/150‑1 (MQ=255)
                                      cACCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCgg                                                                                                            <  1:7545485/150‑1 (MQ=255)
                                       aCCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCggg                                                                                                           <  1:6559896/150‑1 (MQ=255)
                                          ctcctGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGgaga                                                                                                        >  2:3583395/1‑150 (MQ=255)
                                           tcctGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGaa                                                                                                       >  2:6902024/1‑150 (MQ=255)
                                              tGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACa                                                                                                    >  2:8054354/1‑150 (MQ=255)
                                                gCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGc                                                                                                  <  1:7215934/150‑1 (MQ=255)
                                                 cGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCa                                                                                                 <  1:3960769/150‑1 (MQ=255)
                                                 cGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCa                                                                                                 <  1:9151788/150‑1 (MQ=255)
                                                  ggTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCAt                                                                                                >  2:1046852/1‑150 (MQ=255)
                                                     cACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGAc                                                                                             <  2:8086596/150‑1 (MQ=255)
                                                       ccGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGAccc                                                                                           <  1:8051644/150‑1 (MQ=255)
                                                           ccTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGaa                                                                                       >  1:3387646/1‑150 (MQ=255)
                                                           ccTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGaa                                                                                       >  1:895062/1‑150 (MQ=255)
                                                            cTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGaaa                                                                                      <  2:9010948/150‑1 (MQ=255)
                                                             tGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAg                                                                                     >  2:1786957/1‑150 (MQ=255)
                                                              gACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAgg                                                                                    >  1:5023382/1‑150 (MQ=255)
                                                                    tCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGAc                                                                              <  1:11094236/150‑1 (MQ=255)
                                                                    tCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGAc                                                                              <  1:8345218/150‑1 (MQ=255)
                                                                     cGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGAcc                                                                             <  2:2696899/150‑1 (MQ=255)
                                                                      gggAGGCGTACTATGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTGATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCACTTTTTTCCTCCTGCGGGAGTATCAGCTTGACCCTGATTGGCAAGACCg                                                                            <  2:5885238/150‑1 (MQ=255)
                                                                      gggAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCg                                                                            >  2:3936307/1‑150 (MQ=255)
                                                                      gggAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCg                                                                            >  1:5204041/1‑150 (MQ=255)
                                                                      gggAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCg                                                                            >  1:10806724/1‑150 (MQ=255)
                                                                       ggAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGc                                                                           >  1:7697749/1‑150 (MQ=255)
                                                                        gAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGcc                                                                          <  1:3671777/150‑1 (MQ=255)
                                                                        gAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGcc                                                                          >  1:9848932/1‑150 (MQ=255)
                                                                        gAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGcc                                                                          >  1:2846065/1‑150 (MQ=255)
                                                                         aGGCGTACATGGACATTTTCGGCATTCCCCTGATCAAGAGCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGATACAGCTTGACCCTGAAAGGCATGACCGccc                                                                         <  2:9379885/150‑1 (MQ=255)
                                                                         aGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGccc                                                                         <  2:4660175/150‑1 (MQ=255)
                                                                          ggCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCt                                                                        <  1:4508022/150‑1 (MQ=255)
                                                                          ggCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCt                                                                        <  1:7407189/150‑1 (MQ=255)
                                                                           gCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTg                                                                       >  2:7739539/1‑150 (MQ=255)
                                                                           gCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTg                                                                       >  1:7812397/1‑150 (MQ=255)
                                                                              tACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTc                                                                    >  1:8919824/1‑150 (MQ=255)
                                                                                cAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCAc                                                                  <  1:9975668/150‑1 (MQ=255)
                                                                                cAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCAc                                                                  >  1:8748656/1‑150 (MQ=255)
                                                                                cAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCAc                                                                  >  1:6326379/1‑150 (MQ=255)
                                                                                 aaGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCAcc                                                                 >  2:4844926/1‑150 (MQ=255)
                                                                                  aGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCg                                                                >  2:10067697/1‑150 (MQ=255)
                                                                                   ggACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCgg                                                               >  1:4190220/1‑150 (MQ=255)
                                                                                            cGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAg                                                      <  1:7057374/150‑1 (MQ=255)
                                                                                               cATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCgg                                                   <  2:3254533/150‑1 (MQ=255)
                                                                                               cATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCgg                                                   <  2:5197310/150‑1 (MQ=255)
                                                                                                aTTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGc                                                  >  2:6625481/1‑150 (MQ=255)
                                                                                                 ttACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCa                                                 >  2:2807351/1‑150 (MQ=255)
                                                                                                  tACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCAt                                                <  1:6768529/150‑1 (MQ=255)
                                                                                                   aCCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATc                                               <  2:3678353/150‑1 (MQ=255)
                                                                                                     ccTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCgg                                             <  2:1485419/150‑1 (MQ=255)
                                                                                                      cTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGc                                            >  1:2255156/1‑150 (MQ=255)
                                                                                                        gATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCt                                          >  1:10088638/1‑150 (MQ=255)
                                                                                                        gATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCt                                          <  2:11100195/150‑1 (MQ=255)
                                                                                                        gATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCt                                          <  1:4913963/150‑1 (MQ=255)
                                                                                                        gATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCt                                          <  1:698169/150‑1 (MQ=255)
                                                                                                          tCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGACAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTgg                                        >  2:7941309/1‑150 (MQ=255)
                                                                                                          tCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTgg                                        >  2:9552572/1‑150 (MQ=255)
                                                                                                            aaGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTGGGc                                      >  1:3119042/1‑150 (MQ=255)
                                                                                                            aaGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTGGGc                                      >  1:1510479/1‑150 (MQ=255)
                                                                                                             aGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTGGGCa                                     >  1:5122807/1‑150 (MQ=255)
                                                                                                              gACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTGGGCAt                                    <  2:8838277/150‑1 (MQ=255)
                                                                                                               aCCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTGGGCATc                                   >  2:2197499/1‑150 (MQ=255)
                                                                                                                   tGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTGGGCATCGccc                               <  1:1700058/150‑1 (MQ=255)
                                                                                                                      cGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTGGGCATCGCCCTGa                            <  1:1347849/150‑1 (MQ=255)
                                                                                                                       ggTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTGGGCATCGCCCTGAg                           >  2:7035482/1‑150 (MQ=255)
                                                                                                                       ggTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTGGGCATCGCCCTGAg                           <  2:4802215/150‑1 (MQ=255)
                                                                                                                              ttcGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTGGGCATCGCCCTGAGCCTGGcc                    >  1:2198510/1‑150 (MQ=255)
                                                                                                                                cGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCCGCGGCATCGGCCTGGGCATCGCCCTGAGCCTGGCCGa                  >  2:202100/1‑150 (MQ=255)
                                                                                                                                 gTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTGGGCATCGCCCTGAGCCTGGCCGAg                 >  2:11177825/1‑150 (MQ=255)
                                                                                                                                  tCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTGGGCATCGCCCTGAGCCTGGCCGAgg                >  1:542153/1‑150 (MQ=255)
                                                                                                                                  tCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTGGGCATCGCCCTGAGCCTGGCCGAgg                <  2:2405546/150‑1 (MQ=255)
                                                                                                                                    aTCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTGGGCATCGCCCTGAGCCTGGCCGAGGcc              <  2:4237712/150‑1 (MQ=255)
                                                                                                                                     tCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTGGGCATCGCCCTGAGCCTGGCCGAGGCCg             >  2:10783345/1‑150 (MQ=255)
                                                                                                                                     tCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTGGGCATCGCCCTGAGCCTGGCCGAGGCCg             >  1:8008684/1‑150 (MQ=255)
                                                                                                                                          cTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTGGGCATCGCCCTGAGCCTGGCCGAGGCCGGCGcc        <  1:5720506/150‑1 (MQ=255)
                                                                                                                                              tCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTGGGCATCGCCCTGAGCCTGGCCGAGGCCGGCGCCGAcc    >  2:10507790/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GGCGGGATGGATACCCTGCCGCACAACGGCGCGGTGATCACCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACCCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTGGGCATCGCCCTGAGCCTGGCCGAGGCCGGCGCCGACCTG  >  NC_008463/3443145‑3443438

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑