Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 3,670,384 A→G Q128Q (CAA→CAG)  PA14_RS16675 → ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084633,670,3840AG99.5% 1490.3 / NA 386Q128Q (CAA→CAG) PA14_RS16675ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (200/184);  minor base T (2/0);  total (202/184)
Fisher's exact test for biased strand distribution p-value = 5.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 386 total aligned reads displayed.

CCCCTACATCAGCCAACTGATCGCCGAGAAGGACGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAAGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCA  >  NC_008463/3670237‑3670530
                                                                                                                                                   |                                                                                                                                                  
ccccTACATCAGCCAACTGATCGCCGAGAAGGACGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGc                                                                                                                                                  >  2:2454925/1‑150 (MQ=255)
   cTACATCAGCCAACTGATCGCCGAGAAGGACGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCcg                                                                                                                                               <  1:8626327/150‑1 (MQ=255)
     aCATCAGCCAACTGATCGCCGAGAAGGACGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGACGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCcgcg                                                                                                                                             >  2:8394805/1‑150 (MQ=255)
     aCATCAGCCAACTGATCGCCGAGAAGGACGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCcgcg                                                                                                                                             >  1:1812039/1‑150 (MQ=255)
        tCAGCCAACTGATCGCCGAGAAGGACGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACg                                                                                                                                          >  1:1085836/1‑150 (MQ=255)
        tCAGCCAACTGATCGCCGAGAAGGACGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACg                                                                                                                                          >  2:1889101/1‑150 (MQ=255)
            ccAACTGATCGCCGAGAAGGACGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCt                                                                                                                                      <  1:4497438/150‑1 (MQ=255)
             cAACTGATCGCCGAGAAGGACGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTc                                                                                                                                     <  2:9045579/150‑1 (MQ=255)
             cAACTGATCGCCGAGAAGGACGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTc                                                                                                                                     <  1:2539702/150‑1 (MQ=255)
              aaCTGATCGCCGAGAAGGACGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCg                                                                                                                                    <  2:4936766/150‑1 (MQ=255)
               aCTGATCGCCGAGAAGGACGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGc                                                                                                                                   >  1:1463429/1‑150 (MQ=255)
               aCTGATCGCCGAGAAGGACGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGc                                                                                                                                   >  2:1322792/1‑150 (MQ=255)
                 tGATCGCCGAGAAGGTCGGCTGGGTGCTTTGGCCGCCGATCCCCTCCCTCTACGACACCAGAACCTCCGAGCTGCAGGTTCCCGACCCCGCCCCGCCGTCGGCGGAAAACCGGCTGGGCACCGCCGACCAGGCCCGCGACGTGCTCGccc                                                                                                                                 <  2:675658/150‑1 (MQ=255)
                   aTCGCCGAGAAGGACGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCgg                                                                                                                               <  1:7482239/150‑1 (MQ=255)
                   aTCGCCGAGAAGGACGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCgg                                                                                                                               >  1:11084685/1‑150 (MQ=255)
                    tCGCCGAGAAGGACGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCggg                                                                                                                              <  1:2080358/150‑1 (MQ=255)
                       ccGAGAAGGACGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGa                                                                                                                           >  1:9587644/1‑150 (MQ=255)
                        cGAGAAGGACGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGAt                                                                                                                          >  1:1649795/1‑150 (MQ=255)
                         gagaAGGACGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGAtt                                                                                                                         <  2:744728/150‑1 (MQ=255)
                         gagaAGGACGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGAtt                                                                                                                         <  1:1237975/150‑1 (MQ=255)
                          agaAGGACGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGAttt                                                                                                                        >  2:8622853/1‑150 (MQ=255)
                          agaAGGACGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGAttt                                                                                                                        >  2:1952296/1‑150 (MQ=255)
                            aaGGACGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTAc                                                                                                                      >  1:10271363/1‑150 (MQ=255)
                                   gCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGGTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACAGAAGACCAGGCCCGCGACGAGCTCGCCCGGGTGATTTACGGCTTcg                                                                                                               >  2:706436/1‑149 (MQ=255)
                                         tGCTTTGGCCGCCGATCCCCTCCAGCTACGACACCATCAACTAAGAGCTGAAGGTTACCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCgtgt                                                                                                         <  1:2365366/150‑1 (MQ=255)
                                         tGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCgtgt                                                                                                         <  1:5049967/150‑1 (MQ=255)
                                          gCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTc                                                                                                        >  1:4660616/1‑150 (MQ=255)
                                           cTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCg                                                                                                       >  2:2039136/1‑150 (MQ=255)
                                            tttGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGa                                                                                                      <  1:6484404/150‑1 (MQ=255)
                                             ttGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGAt                                                                                                     <  1:3846922/150‑1 (MQ=255)
                                               ggCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATcc                                                                                                   <  2:5365161/150‑1 (MQ=255)
                                                 ccgccgATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCctc                                                                                                 <  2:4326112/150‑1 (MQ=255)
                                                  cgccgATCCCCTACCGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCctct                                                                                                <  2:1038315/150‑1 (MQ=255)
                                                  cgccgATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCctct                                                                                                <  2:291543/150‑1 (MQ=255)
                                                   gccgATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCtt                                                                                               <  1:193732/150‑1 (MQ=255)
                                                   gccgATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCtt                                                                                               >  1:9286531/1‑150 (MQ=255)
                                                        tCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGccc                                                                                          >  1:5847087/1‑150 (MQ=255)
                                                         ccccTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGccct                                                                                         >  1:5532284/1‑150 (MQ=255)
                                                          cccTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGccctg                                                                                        <  2:37930/150‑1 (MQ=255)
                                                              aCAGCTACGACACCATCCACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGccctgaccc                                                                                    <  2:5671365/150‑1 (MQ=255)
                                                              aCAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGccctgaccc                                                                                    <  2:5530502/150‑1 (MQ=255)
                                                              aCAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGccctgaccc                                                                                    <  2:5783197/150‑1 (MQ=255)
                                                               cAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGccctgaccct                                                                                   >  2:2687929/1‑150 (MQ=255)
                                                                  cTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCcctgaccctgac                                                                                <  2:8490940/150‑1 (MQ=255)
                                                                  cTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCcctgaccctgac                                                                                >  2:11015320/1‑150 (MQ=255)
                                                                   tACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCctgaccctgacc                                                                               >  2:6549349/1‑150 (MQ=255)
                                                                   tACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCctgaccctgacc                                                                               >  1:8240908/1‑150 (MQ=255)
                                                                   tACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCctgaccctgacc                                                                               >  1:5932218/1‑150 (MQ=255)
                                                                    aCGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCg                                                                              >  2:468530/1‑150 (MQ=255)
                                                                     cGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGCCGACCAGGCCCGCGACGTGCTCGCGCGGGTGATTTACGGCTGCCGCGTGTCGAGCAGCTTCGCCCTGACCCTGACCGt                                                                             >  2:10525865/1‑150 (MQ=25)
                                                                      gACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTg                                                                            <  2:6714268/150‑1 (MQ=255)
                                                                        cacCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCt                                                                          <  2:10696892/150‑1 (MQ=255)
                                                                         acCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTc                                                                         >  1:883537/1‑150 (MQ=255)
                                                                         acCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTc                                                                         >  2:3330196/1‑150 (MQ=255)
                                                                         acCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTc                                                                         >  1:5427781/1‑150 (MQ=255)
                                                                          ccATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCa                                                                        >  1:6335473/1‑150 (MQ=255)
                                                                            aTCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGt                                                                      <  1:6806760/150‑1 (MQ=255)
                                                                             tCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCACAGtt                                                                     <  2:9848225/150‑1 (MQ=255)
                                                                              cAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTtc                                                                    >  1:7685862/1‑150 (MQ=255)
                                                                               aaCTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTtct                                                                   >  2:10829895/1‑150 (MQ=255)
                                                                                  tACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTg                                                                >  2:7324144/1‑150 (MQ=255)
                                                                                   aCGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGAGTCGATCCTCTTCGCCCTGACCCGGACCGGGATCAGTGCTCTGa                                                               >  2:1144644/1‑150 (MQ=25)
                                                                                   aCGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCGTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGa                                                               <  1:10046612/150‑1 (MQ=255)
                                                                                    cGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGAt                                                              <  2:5935941/150‑1 (MQ=255)
                                                                                      aGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCg                                                            >  2:3476206/1‑150 (MQ=255)
                                                                                          gAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGt                                                        <  1:5152463/150‑1 (MQ=255)
                                                                                          gAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGt                                                        >  2:3038298/1‑150 (MQ=255)
                                                                                          gAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGt                                                        >  1:9012564/1‑150 (MQ=255)
                                                                                           aaGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTc                                                       >  1:8091165/1‑150 (MQ=255)
                                                                                           aaGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTc                                                       >  1:8140506/1‑150 (MQ=255)
                                                                                             ggTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCAc                                                     >  1:4105425/1‑150 (MQ=255)
                                                                                             ggTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCAc                                                     <  1:4202638/150‑1 (MQ=255)
                                                                                              gTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCAcc                                                    <  1:3549571/150‑1 (MQ=255)
                                                                                                 cccgcccccgccCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTc                                                 <  2:8025082/150‑1 (MQ=255)
                                                                                                   cgcccccgccccGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCgg                                               <  2:1255936/150‑1 (MQ=255)
                                                                                                       cccgccccgcCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGcc                                           >  1:3014667/1‑150 (MQ=255)
                                                                                                           ccccgccGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCGTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGc                                       <  2:7757612/150‑1 (MQ=255)
                                                                                                             ccgccgTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCaa                                     >  2:10947647/1‑150 (MQ=255)
                                                                                                              cgccgTCGGCGGAAAACTGGCTGGGCACCGACGAACAGGCCCGCGACGTGCTCGACCGGGTGATTTACGGCTACCGCGTCTCGATCCACTTCGCCCTGACCCTGACCGTGCTAAGTTCTCTGAAAGGCGTCACCGTCGGCGCCCTGCAAg                                    >  2:878582/1‑150 (MQ=255)
                                                                                                               gccgTCGGCGGACAACGGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGGGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAgg                                   <  2:4451495/150‑1 (MQ=255)
                                                                                                                ccgTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGc                                  <  1:5376914/150‑1 (MQ=255)
                                                                                                                 cgTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGct                                 <  2:10213825/150‑1 (MQ=255)
                                                                                                                   tCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGctac                               >  1:10670155/1‑150 (MQ=255)
                                                                                                                       cggAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTAcg                           >  1:6158603/1‑150 (MQ=255)
                                                                                                                          aaaaCTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTAcggcg                        <  2:3638864/150‑1 (MQ=255)
                                                                                                                             aCTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCt                     >  2:6084744/1‑150 (MQ=255)
                                                                                                                              ctggctggGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTg                    <  2:9457776/150‑1 (MQ=255)
                                                                                                                                 gctggGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGt                 <  1:3704409/150‑1 (MQ=255)
                                                                                                                                    gggCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGa              <  2:10134280/150‑1 (MQ=255)
                                                                                                                                     ggCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGAc             <  1:141929/150‑1 (MQ=255)
                                                                                                                                      gCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGAcc            <  1:9075481/150‑1 (MQ=255)
                                                                                                                                         ccgccgACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGc         <  2:1428824/150‑1 (MQ=255)
                                                                                                                                          cgacgaCCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCt        >  2:6840171/1‑150 (MQ=255)
                                                                                                                                          cgacgaCCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCt        <  1:7180036/150‑1 (MQ=255)
                                                                                                                                           gacgacCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTc       >  1:2877024/1‑150 (MQ=255)
                                                                                                                                             cgacCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCgg     <  2:3700558/150‑1 (MQ=255)
                                                                                                                                              gacCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGc    <  1:9481497/150‑1 (MQ=255)
                                                                                                                                               acCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGcc   >  2:10020887/1‑150 (MQ=255)
                                                                                                                                               acCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGcc   <  2:2170109/150‑1 (MQ=255)
                                                                                                                                                ccAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCa  <  2:9710755/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CCCCTACATCAGCCAACTGATCGCCGAGAAGGACGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAAGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCA  >  NC_008463/3670237‑3670530

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑