Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 3,673,524 A→G intergenic (+73/+25) fabI → / ← PA14_RS16690 enoyl‑ACP reductase FabI/SurA N‑terminal domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084633,673,5240AG100.0% 1631.6 / NA 435intergenic (+73/+25)fabI/PA14_RS16690enoyl‑ACP reductase FabI/SurA N‑terminal domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (227/208);  total (227/208)

Only 100 of 435 total aligned reads displayed.

GGTATCAGTGGCGAGATCCTCTACGTCGATGGCGGCTTCAACACTACCGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGNGTAAACGGCCTTTTTCTTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCTCGTCGTCGGAGATAGCCT  >  NC_008463/3673377‑3673670
                                                                                                                                                   |                                                                                                                                                  
 gTATCAGTGGCGAGATCCTCTACGTCGATGGCGGCTTCAACACTACCGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCgg                                                                                                                                                 <  1:2407256/150‑1 (MQ=255)
 gTATCAGTGGCGAGATCCTCTACGTCGATGGCGGCTTCAACACTACCGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCGTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAATGGCCGCGTAAGCgg                                                                                                                                                 >  1:3856506/1‑150 (MQ=255)
  tATCAGTGGCGAGATCCTCTACGTCGATGGCGGCTTCAACACTACCGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGc                                                                                                                                                <  1:7269262/150‑1 (MQ=255)
   aTCAGTGGCGAGATCCTCTACGTCGATGGCGGCTTCAACACTACCGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGcc                                                                                                                                               <  2:5683370/150‑1 (MQ=255)
   aTCAGTGGCGAGATCCTCTACGTCGATGGCGGCTTCAACACTACCGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGcc                                                                                                                                               >  1:10745825/1‑150 (MQ=255)
   aTCAGTGGCGAGATCCTCTACGTCGATGGCGGCTTCAACACTACCGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGcc                                                                                                                                               <  1:7473357/150‑1 (MQ=255)
    tCAGTGGCGAGATCCTCTACGTCGATGGCGGCTTCAACACTACCGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCt                                                                                                                                              >  2:9763366/1‑150 (MQ=255)
     cAGTGGCGAGATCCTCTACGTCGATGGCGGCTTCAACACTACCGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGTGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTTAGCGGCCtt                                                                                                                                             >  1:5314564/1‑150 (MQ=255)
     cAGTGGCGAGATCCTCTACGTCGATGGCGGCTTCAACACTACCGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCtt                                                                                                                                             <  1:4332645/150‑1 (MQ=255)
      aGTGGCGAGATCCTCTACGTCGATGGCGGCTTCAACACTACCGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCttt                                                                                                                                            <  1:1694146/150‑1 (MQ=255)
       gTGGCGAGATCCTCTACGTCGATGGCGGCTTCAACACTACCGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAAGAAAGAAAAGGCCGCGTAAGCGGCCttta                                                                                                                                           <  2:4043016/150‑2 (MQ=255)
          gCGAGATCCTCTACGACGATGGCGGCTTCAACCCTACCGCCTTGGGCCCGCTGGACTACGACGGATCACCCCGCTCATTAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCttttctc                                                                                                                                        <  2:826088/150‑4 (MQ=255)
             agaTCCTCTACGTCGATGGCGGCTTCAACACTACCGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCttttctctgc                                                                                                                                     >  1:6120444/1‑144 (MQ=255)
                 ccTCTACGTCGATGGCGGCTTCAACACTACCGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCAtct                                                                                                                                 >  2:8773162/1‑150 (MQ=255)
                   tctACGTCGATGGCGGCTTCAACACTACCGCCCTGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCg                                                                                                                               >  2:3642043/1‑150 (MQ=255)
                      acttCGATGGCGGCTTCAACACTACCGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGtc                                                                                                                            >  2:2303594/4‑150 (MQ=255)
                      aCGTCGATGGCGGCTTCAACACTACCGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCAGCTCGGtc                                                                                                                            >  1:1915356/1‑150 (MQ=255)
                       cGTCGATGGCGGCTTCAACACTACCGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCATTTTTCATGGGCGGAATAAAGAAAAGGCCGCTTAAGCGGCCTTTTCTCTGCATCTCGGtct                                                                                                                           <  1:10875234/150‑1 (MQ=255)
                        gTCGATGGCGGCTTCAACACTACCGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAATTGGCCGCTTCTAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGtctc                                                                                                                          <  1:5956770/150‑1 (MQ=255)
                         tCGATGGCGGCTTCAACACTACCGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCa                                                                                                                         >  1:5147722/1‑150 (MQ=255)
                           gATGGCGGCTTCAACACTACCGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTACTTGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGt                                                                                                                       >  1:7119743/1‑150 (MQ=255)
                             tGGCGGCTTCAACACTACCGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTAt                                                                                                                     >  2:3372892/1‑150 (MQ=255)
                             tGGCGGCTTCAACACTACCGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTAt                                                                                                                     >  1:7899913/1‑150 (MQ=255)
                                 ggcTTCAACACTACCCCCATGGGCCCGCTGGACGCCGACTTATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTACTGGGCGGAATAAAGAAATGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTtct                                                                                                                 <  1:4797478/150‑1 (MQ=255)
                                 ggcTACAACACTACCGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATCAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTtct                                                                                                                 <  1:8638171/150‑1 (MQ=255)
                                   cTTCAACACTACCGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCg                                                                                                               >  2:4304337/1‑150 (MQ=255)
                                    ttCAACACTACCGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGa                                                                                                              <  1:1519986/150‑1 (MQ=255)
                                     tCAACACTACCGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGAc                                                                                                             >  2:2674199/1‑150 (MQ=255)
                                     tCAACACTACCGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGAc                                                                                                             <  1:1616976/150‑1 (MQ=255)
                                        acacTACCCCCATGGGCCCGCGGGACGACGACTGATCACCCCGCTCATGAAAAAGGGCCGCTTCGAGCGGCCTTTTTCCTGGGCGGAATAAACAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTc                                                                                                          <  1:9466663/150‑1 (MQ=255)
                                          cctcccGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAATAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCg                                                                                                        <  1:4900839/146‑1 (MQ=255)
                                           cgcccGCCAGGGGCCCGCTGCACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAACAACCGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGc                                                                                                       <  1:6534597/147‑1 (MQ=255)
                                            tACCGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGcc                                                                                                      <  1:5862710/150‑1 (MQ=255)
                                               cGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTg                                                                                                   <  1:3280113/150‑1 (MQ=255)
                                                   aTGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCACGGTCTCAGTATTTCTCGACTTACGCCTTGTCCt                                                                                               >  1:1130413/1‑150 (MQ=255)
                                                   aTGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCATTTCTCTGCATCACGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCt                                                                                               <  2:11095438/150‑1 (MQ=255)
                                                    tGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTg                                                                                              >  2:7490583/1‑150 (MQ=255)
                                                      ggCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCa                                                                                            <  2:4663760/150‑1 (MQ=255)
                                                         ccGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACt                                                                                         <  1:7996013/150‑1 (MQ=255)
                                                         ccGCTGGACGACGACGGATCACCCCGCTCAAGAAAATTGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACt                                                                                         <  1:10405280/150‑1 (MQ=255)
                                                            cTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAACGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGAc                                                                                      <  1:10598645/150‑1 (MQ=255)
                                                               gacgacgacTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGAcggc                                                                                   <  1:6017667/150‑1 (MQ=255)
                                                               gacgacgacTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGAcggc                                                                                   <  2:299150/150‑1 (MQ=255)
                                                                acgacgacAGATCACCCCGCTCCTGAAAAATGGCCGCTTCGAGCGGCCTATTTCATGGGCGGAATAAAGACAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGAcggcg                                                                                  <  1:8180341/150‑1 (MQ=255)
                                                                     gacTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAACGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAgg                                                                             <  2:77620/150‑1 (MQ=255)
                                                                     gacTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAgg                                                                             >  2:6945668/1‑150 (MQ=255)
                                                                      acTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAggc                                                                            >  2:1678829/1‑150 (MQ=255)
                                                                      acTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGAGGACTAAAGAAAAGTCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCTTCTTCCGCCTTGTCCTGCACCTGACGGCGGAAggc                                                                            >  2:988164/1‑150 (MQ=255)
                                                                         gATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAggcggc                                                                         >  1:5559978/1‑150 (MQ=255)
                                                                         gATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAggcggc                                                                         >  2:5920680/1‑150 (MQ=255)
                                                                              ccccGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGt                                                                    >  1:3777891/1‑150 (MQ=255)
                                                                               cccGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTc                                                                   <  2:10165786/150‑1 (MQ=255)
                                                                                ccGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCt                                                                  >  2:8906751/1‑150 (MQ=255)
                                                                                 cGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTg                                                                 <  1:9209043/150‑1 (MQ=255)
                                                                                  gCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAACCGGCCGCGTAAGCGGCCATTTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGc                                                                <  1:1318787/150‑1 (MQ=255)
                                                                                       tGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTc                                                           <  2:3922471/150‑1 (MQ=255)
                                                                                       tGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTc                                                           >  2:6277434/1‑150 (MQ=255)
                                                                                       tGAAAAAGGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCCTCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTc                                                           <  2:308661/150‑1 (MQ=255)
                                                                                         aaaaaTGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGACGGCGGCGAAGTCTGCCTGTCCg                                                         >  1:6991645/1‑150 (MQ=255)
                                                                                           aaaGGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGCAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCt                                                       <  2:9292856/150‑1 (MQ=255)
                                                                                            aagggCCGCATCGCGCGGCCTCTTTCATGGGCGGACTACAGAAAAGGCCGCGTAAGCGGCCTTATCTCTGCCTCACGGTCTCAGTACTTCGCGACTTCCGCCATGGCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTg                                                      <  2:6310157/147‑1 (MQ=255)
                                                                                              tGGCCGCTTCGAGCGGCCATATTCATGGGCGGAATAAAGCAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATGTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTgcg                                                    <  2:11109118/150‑1 (MQ=255)
                                                                                                   gCTTCGAGCGGCCTTTTTCAGGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAg                                               <  1:8460633/150‑1 (MQ=255)
                                                                                                    cTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGc                                              >  1:9734622/1‑150 (MQ=255)
                                                                                                       cGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAg                                           <  2:10719048/150‑1 (MQ=255)
                                                                                                        gAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAgg                                          >  1:2147515/1‑150 (MQ=255)
                                                                                                         aGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGa                                         <  2:1541381/150‑1 (MQ=255)
                                                                                                         aGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGa                                         >  2:4189004/1‑150 (MQ=255)
                                                                                                         aGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGa                                         <  2:213693/150‑1 (MQ=255)
                                                                                                         aGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGa                                         >  1:6813583/1‑150 (MQ=255)
                                                                                                          gCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGaa                                        >  1:6680259/1‑150 (MQ=255)
                                                                                                            ggCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAgc                                      >  1:3093717/1‑150 (MQ=255)
                                                                                                            ggCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAgc                                      <  1:6316987/150‑1 (MQ=255)
                                                                                                             gCCTTTTTCATGGGCGGAATACAGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAgcg                                     <  1:10184050/150‑1 (MQ=255)
                                                                                                               cTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAgcggc                                   >  1:548267/1‑150 (MQ=255)
                                                                                                               cTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTGTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAgcggc                                   >  1:9077560/1‑150 (MQ=255)
                                                                                                                tttttCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAgcggcg                                  >  1:6358045/1‑150 (MQ=255)
                                                                                                                tttttCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAgcggcg                                  >  1:4648807/1‑150 (MQ=255)
                                                                                                                      aTGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACAt                            <  2:6715398/150‑1 (MQ=255)
                                                                                                                       tGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATg                           >  1:3639069/1‑150 (MQ=255)
                                                                                                                        gggCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATgg                          >  2:4883364/1‑150 (MQ=255)
                                                                                                                         ggCGGAATCAAGAAACGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGc                         <  2:9452980/150‑1 (MQ=255)
                                                                                                                         ggCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGACGCGGCGGTACATGGc                         >  2:1054890/1‑150 (MQ=255)
                                                                                                                            ggAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCtt                      >  2:1768171/1‑150 (MQ=255)
                                                                                                                            ggAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCtt                      >  1:7656328/1‑150 (MQ=255)
                                                                                                                              aaTAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTtct                    <  2:5098588/150‑1 (MQ=255)
                                                                                                                               aTAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGACGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTtctc                   >  2:10300607/1‑150 (MQ=255)
                                                                                                                               aTAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCGtctc                   >  2:965060/1‑150 (MQ=255)
                                                                                                                                 aaaGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCtcgt                 >  1:285641/1‑150 (MQ=255)
                                                                                                                                  aaGAACAGGCCGCGTAAGCGGCCTTTTCTCTGCCTCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCtcgtc                <  1:898671/150‑1 (MQ=255)
                                                                                                                                  aaGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCtcgtc                <  2:7286032/150‑1 (MQ=255)
                                                                                                                                  aaGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCtcgtc                >  2:1705423/1‑150 (MQ=255)
                                                                                                                                   aGAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCtcgtcg               >  1:3448645/1‑150 (MQ=255)
                                                                                                                                    gAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCtcgtcgt              >  2:8076841/1‑150 (MQ=255)
                                                                                                                                    gAAAAGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCtcgtcgt              <  1:9982376/150‑1 (MQ=255)
                                                                                                                                       caGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCTCGTCGTCgg           <  1:4132577/149‑1 (MQ=255)
                                                                                                                                        aGGCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCTCGTCGTCGga          >  2:10862163/1‑150 (MQ=255)
                                                                                                                                         ggCCGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCTCGTCGTCGgag         >  2:2000738/1‑150 (MQ=255)
                                                                                                                                           ccGCGTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCTCGTCGTCGGAGAt       <  2:8847791/150‑1 (MQ=255)
                                                                                                                                            cgcgTAAGCGGCCTTTTCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCTCGTCGTCGGAGATa      <  1:832836/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GGTATCAGTGGCGAGATCCTCTACGTCGATGGCGGCTTCAACACTACCGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGNGTAAACGGCCTTTTTCTTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCTCGTCGTCGGAGATAGCCT  >  NC_008463/3673377‑3673670

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑