Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 4,760,743 A→G L73P (CTT→CCT)  PA14_RS21850 ← HNH endonuclease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084634,760,7430AG100.0% 1661.0 / NA 418L73P (CTT→CCT) PA14_RS21850HNH endonuclease
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (179/239);  total (179/239)

Only 100 of 428 total aligned reads displayed.

ATCTCCCGGACACCACTACGCCAGTAGTTGCTGGCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAAGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCACCCCTGGCTTAAGGATGGCGCTGTATCGCCTT  >  NC_008463/4760596‑4760889
                                                                                                                                                   |                                                                                                                                                  
 tctcCCGGACACCACTACGCCAGTAGTTGCTGGCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGt                                                                                                                                                 >  1:5393107/1‑150 (MQ=255)
 tctcCCGGACACCACTACGCCAGTAGTTGCTGGCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGt                                                                                                                                                 <  1:5772688/150‑1 (MQ=255)
 tctcCCGGACACCACTACGCCAGTAGTTGCTGGCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGt                                                                                                                                                 >  2:6302011/1‑150 (MQ=255)
  ctcCCGGACACCACTACGCCAGTAGTTGCTGGCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTc                                                                                                                                                >  1:957754/1‑150 (MQ=255)
   tcCCGGACACCACTACGCCAGTAGTTGCTGGCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCa                                                                                                                                               <  2:6708347/150‑1 (MQ=255)
   tcCCGGACACCACTACGCCAGTAGTTGCTGGCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCa                                                                                                                                               >  1:7261812/1‑150 (MQ=255)
   tcCCGGACACCACTACGCCAGTAGTTGCTGGCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCa                                                                                                                                               <  1:9520524/150‑1 (MQ=255)
    cccGGACACCACTACGCCAGTAGTTGCTGGCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAg                                                                                                                                              >  1:4082987/1‑150 (MQ=255)
     ccGGACACCACTACGCCAGTAGTTGCTGGCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAgg                                                                                                                                             <  2:7408660/150‑1 (MQ=255)
        gACACCACTACGCCAGTAGTTGCTGGCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTa                                                                                                                                          <  2:10115926/150‑1 (MQ=255)
           accacTACGCCAGTAGTTGCTGGCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAAc                                                                                                                                       >  2:1185874/1‑150 (MQ=255)
            ccacTACGCCAGTAGTTGCTGGCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAAcc                                                                                                                                      <  1:152822/150‑1 (MQ=255)
             cacTACGCCAGTAGTTGCTGGCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCt                                                                                                                                     <  1:9220030/150‑1 (MQ=255)
               cTACGCCAGTAGTTGCTGGCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTg                                                                                                                                   >  2:5025626/1‑150 (MQ=255)
               cTACGCCAGTAGTTGCTGGCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTg                                                                                                                                   >  1:4311334/1‑150 (MQ=255)
                     cAGTAGTTGCTGGCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATa                                                                                                                             <  1:4018843/150‑1 (MQ=255)
                        tagtTGCTGGCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATAcgc                                                                                                                          <  1:10558019/150‑1 (MQ=255)
                          gtTGCTGGCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACgcgc                                                                                                                        <  1:3637994/150‑1 (MQ=255)
                          gtTGCTGGCCTTCGATGGCGGAAGGGGCTCTAGGTAGCTATTCCCTTCTTGTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACgcgc                                                                                                                        <  2:5710563/150‑1 (MQ=255)
                              cTGGCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATa                                                                                                                    >  1:8377538/1‑150 (MQ=255)
                                ggCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATAcc                                                                                                                  <  1:7729913/150‑1 (MQ=255)
                                     tCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGt                                                                                                             >  1:8916946/1‑150 (MQ=255)
                                     tCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGt                                                                                                             <  1:1114809/150‑1 (MQ=255)
                                       gATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTaa                                                                                                           >  1:2259889/1‑150 (MQ=255)
                                        aTGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAAt                                                                                                          >  2:5613346/1‑150 (MQ=255)
                                          ggCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGc                                                                                                        >  1:2979069/1‑150 (MQ=255)
                                              gAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCt                                                                                                    <  1:10220765/150‑1 (MQ=255)
                                                aTGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGa                                                                                                  <  2:761782/150‑1 (MQ=255)
                                                    gCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCAc                                                                                              <  1:8003087/150‑1 (MQ=255)
                                                     ctctAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACt                                                                                             >  2:3699925/1‑150 (MQ=255)
                                                     ctctAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACt                                                                                             <  1:7103909/150‑1 (MQ=255)
                                                       ctAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGa                                                                                           >  2:6562644/1‑150 (MQ=255)
                                                       ctAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGa                                                                                           <  2:3282953/150‑1 (MQ=255)
                                                       ctAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGa                                                                                           >  1:7207755/1‑150 (MQ=255)
                                                         aGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAgg                                                                                         <  2:5139237/150‑1 (MQ=255)
                                                         aGGTAGCTATTCCCTTCATTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCACAGTAATGCGGCTGAGCACTGAgg                                                                                         <  2:4768370/150‑1 (MQ=255)
                                                          ggTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAAGGTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGgc                                                                                        <  1:6747863/150‑1 (MQ=255)
                                                          ggTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGgc                                                                                        >  2:4017880/1‑150 (MQ=255)
                                                           gTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGgcg                                                                                       <  2:2352250/150‑1 (MQ=255)
                                                            tAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGgcgc                                                                                      <  1:2568454/150‑1 (MQ=255)
                                                              gCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTg                                                                                    >  1:9690219/1‑150 (MQ=255)
                                                               cTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGa                                                                                   <  2:8058860/150‑1 (MQ=255)
                                                                 aTTCCCTTCTTTTTCCAGGACTGCCTGCTCAAATGGCTGACAACTCTCGATCAACGCGAAGAGATCACCTTTCGCGCTTTCAGGGTCAGGGTAATCCTTGCCGATACGCGCAATTCCAACGTAATGCGGCTGAGCACTGAGGCGCTGATc                                                                                 <  1:912875/150‑1 (MQ=255)
                                                                  ttCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCa                                                                                >  2:8097275/1‑150 (MQ=255)
                                                                  ttCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCa                                                                                <  2:613670/150‑1 (MQ=255)
                                                                   tCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAg                                                                               <  2:9493019/150‑1 (MQ=255)
                                                                     ccTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCa                                                                             >  2:7129624/1‑150 (MQ=255)
                                                                      cttcttTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCaa                                                                            <  1:5516496/150‑1 (MQ=255)
                                                                          ttttGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGa                                                                        <  2:4252648/150‑1 (MQ=255)
                                                                             tGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACtt                                                                     <  2:6212367/150‑1 (MQ=255)
                                                                              gCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTa                                                                    <  2:9107852/150‑1 (MQ=255)
                                                                              gCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTa                                                                    >  1:6052063/1‑150 (MQ=255)
                                                                               ccAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTAt                                                                   <  2:10855696/150‑1 (MQ=255)
                                                                                cAGGACTGCCTGCTCAACTGGCTGATAACTCTCGCTCAACGCGTAGAGATCACCTGTCGAGCTTTCAGGGTCAGGGTCAACCTTGCCGATACGCGCAATCCCACAGTACTGCGGCTGACCACTGAGGCGCTGATCAGCAAAGGTCGTATc                                                                  <  2:7745002/150‑1 (MQ=255)
                                                                                cAGGACTGCCAGCTCAAATGGCTGAAAACTCTCGATCACCGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATc                                                                  <  1:3247689/150‑1 (MQ=255)
                                                                                 aGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCt                                                                 <  1:5066299/150‑1 (MQ=255)
                                                                                  ggACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTg                                                                >  2:8756380/1‑150 (MQ=255)
                                                                                    aCTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTc                                                              <  2:6044807/150‑1 (MQ=255)
                                                                                    aCTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTc                                                              <  1:7919287/150‑1 (MQ=255)
                                                                                       gccagcTCAACTGGCTGAAAACTCTCGAACAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGc                                                           <  2:10295558/150‑1 (MQ=255)
                                                                                        cctgcTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCt                                                          >  1:10007158/1‑150 (MQ=255)
                                                                                         ctgcTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCtt                                                         <  1:391195/150‑1 (MQ=255)
                                                                                             tCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGccc                                                     >  1:4570372/1‑150 (MQ=255)
                                                                                               aaaTGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCtt                                                   >  1:5615886/1‑150 (MQ=255)
                                                                                                aaTGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTg                                                  >  2:4573827/1‑150 (MQ=255)
                                                                                                aaTGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTg                                                  >  1:8464614/1‑150 (MQ=255)
                                                                                                   ggCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTgtag                                               >  2:10563300/1‑150 (MQ=255)
                                                                                                    gCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTgtagt                                              <  1:6503042/150‑1 (MQ=255)
                                                                                                       gAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTaaa                                           <  1:11024298/150‑1 (MQ=255)
                                                                                                        aaaaCTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAac                                          >  1:6312765/1‑150 (MQ=255)
                                                                                                          aaCTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAacca                                        <  2:5923369/150‑1 (MQ=255)
                                                                                                            ctctCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAaccacc                                      <  2:1801246/150‑1 (MQ=255)
                                                                                                             tctcGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCt                                     <  2:2861952/150‑1 (MQ=255)
                                                                                                              ctcGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTg                                    <  2:8317638/150‑1 (MQ=255)
                                                                                                              ctcGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTg                                    >  1:9872037/1‑150 (MQ=255)
                                                                                                                cGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCa                                  <  2:10634959/150‑1 (MQ=255)
                                                                                                                    cAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCAcccc                              <  2:5351072/150‑1 (MQ=255)
                                                                                                                    cAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCAcccc                              >  1:2752402/1‑150 (MQ=255)
                                                                                                                    cAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCAcccc                              <  1:2020944/150‑1 (MQ=255)
                                                                                                                     aaCGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCACCCCt                             >  1:1813223/1‑150 (MQ=255)
                                                                                                                        gcgAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCACCCCTGGc                          <  2:14664/150‑1 (MQ=255)
                                                                                                                        gcgAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCACCCCTGGc                          <  1:8654713/150‑1 (MQ=255)
                                                                                                                         cgAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCACCCCTGGCt                         >  1:11170179/1‑150 (MQ=255)
                                                                                                                         cgAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCACCCCTGGCt                         <  1:4530533/150‑1 (MQ=255)
                                                                                                                         cgAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCACCCCTGGCt                         <  1:4656388/150‑1 (MQ=255)
                                                                                                                          gAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGATGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCACCCCTGGCtt                        >  1:3396039/1‑150 (MQ=255)
                                                                                                                           aaGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCACCCCTGGCTTa                       >  2:3526475/1‑150 (MQ=255)
                                                                                                                           aaGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCACCCCTGGCTTa                       >  1:6964028/1‑150 (MQ=255)
                                                                                                                            agagATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCACCCCTGGCTTaa                      <  2:6913112/150‑1 (MQ=255)
                                                                                                                             gagaTCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCACCCCTGGCTTAAg                     >  2:9289815/1‑150 (MQ=255)
                                                                                                                             gagaTCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCACCCCTGGCTTAAg                     <  2:2682817/150‑1 (MQ=255)
                                                                                                                             gagaTCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCACCCCTGGCTTAAg                     >  1:113576/1‑150 (MQ=255)
                                                                                                                             gagaTCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCACCCCTGGCTTAAg                     <  1:4792527/150‑1 (MQ=255)
                                                                                                                                       ttCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCACCCCTGGCTTAAGGATGGCGCTg           >  2:9731080/1‑150 (MQ=255)
                                                                                                                                       ttCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCACCCCTGGCTTAAGGATGGCGCTg           <  1:927610/150‑1 (MQ=255)
                                                                                                                                        tCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGAAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCACCCCTGGCTTAAGGATGGCGCTGt          >  2:2302217/1‑150 (MQ=255)
                                                                                                                                         cGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCACCCCTGGCTTAAGGATGGCGCTGTa         <  1:1754765/150‑1 (MQ=255)
                                                                                                                                             cTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCACCCCTGGCTTAAGGATGGCGCTGTATCGc     >  2:8019113/1‑150 (MQ=255)
                                                                                                                                              tttCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCACCCCTGGCTTAAGGATGGCGCTGTATCGcc    <  1:4969284/150‑1 (MQ=255)
                                                                                                                                               ttCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCACCCCTGGCTGAAGGATGGCGCTGTATCGCCt   >  2:9616049/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
ATCTCCCGGACACCACTACGCCAGTAGTTGCTGGCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAAGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCACCCCTGGCTTAAGGATGGCGCTGTATCGCCTT  >  NC_008463/4760596‑4760889

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑