Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 4,997,786 T→C K173E (AAG→GAG)  PA14_RS22840 ← DUF853 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084634,997,7860TC100.0% 1867.7 / NA 472K173E (AAG→GAG) PA14_RS22840DUF853 family protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (222/250);  total (222/250)

Only 100 of 472 total aligned reads displayed.

GCCCCCGCCCGTCCGGCTCGGGGCGGAGGATGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTTGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAGCGCGGCCTGCTGGCTATCGGTCAACTCCAGGAGGTTGG  >  NC_008463/4997639‑4997932
                                                                                                                                                   |                                                                                                                                                  
gcCCCCGCCCGTCCGGCTCGGGGCGGAGGATGTCTTCCAGTGGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCCGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGc                                                                                                                                                  <  1:3792122/150‑1 (MQ=255)
  ccccGCCCGTCCGGCTCGGGGCGGAGGATGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCg                                                                                                                                                <  1:6071519/150‑1 (MQ=255)
      gcccgTCCGGCTCGGGGCGGAGGATGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGa                                                                                                                                            <  1:6437165/150‑1 (MQ=255)
       cccgTCCGGCTCGGGGCGGAGGATGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGAt                                                                                                                                           >  1:10921196/1‑150 (MQ=255)
       cccgTCCGGCTCGGGGCGGAGGATGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGAt                                                                                                                                           >  2:8683865/1‑150 (MQ=255)
        ccgTCCGGCTCGGGGCGGAGGATGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGAtc                                                                                                                                          <  2:9826614/150‑1 (MQ=255)
        ccgTCCGGCTCGGGGCGGAGGATGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGAtc                                                                                                                                          <  2:5474608/150‑1 (MQ=255)
         cgTCCGGCTCGGGGCGGAGGATGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGAtcg                                                                                                                                         >  1:457847/1‑149 (MQ=255)
            ccGGCTCGGGGCGGAGGATGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCg                                                                                                                                      <  1:9110605/150‑1 (MQ=255)
            ccGGCTCGGGGCGGAGGATGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCg                                                                                                                                      <  1:4100356/150‑1 (MQ=255)
              ggCTCGGGGCGGAGGATGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGa                                                                                                                                    <  2:7890717/150‑1 (MQ=255)
              ggCTCGGGGCGGAGGATGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGa                                                                                                                                    >  2:1201856/1‑150 (MQ=255)
                cTCGGGGCGGAGGATGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGAtt                                                                                                                                  >  1:10722121/1‑150 (MQ=255)
                cTCGGGGCGGAGGATGTCGTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGagt                                                                                                                                  >  2:6947375/1‑148 (MQ=255)
                 tCGGGGCGGAGGATGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTg                                                                                                                                 >  1:6951022/1‑150 (MQ=255)
                 tCGGGGCGGAGGATGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTg                                                                                                                                 >  1:6185767/1‑150 (MQ=255)
                    gggCGGAGGATGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTcc                                                                                                                              >  2:4438132/1‑150 (MQ=255)
                    gggCGGAGGATGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTcc                                                                                                                              <  2:8756552/150‑1 (MQ=255)
                      gCGGAGGCTGTCTTCCAGGTGCCGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCAGCTCCAGAGTGGCCCGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCtt                                                                                                                            <  2:10859453/150‑1 (MQ=255)
                        ggaggaTGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCa                                                                                                                          <  1:1808000/150‑1 (MQ=255)
                         gaggaTGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAg                                                                                                                         >  2:5603929/1‑150 (MQ=255)
                           ggaTGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAggt                                                                                                                       >  2:1682694/1‑150 (MQ=255)
                           ggaTGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAggt                                                                                                                       >  1:1208134/1‑150 (MQ=255)
                             aTGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAggtgg                                                                                                                     <  1:9168193/150‑1 (MQ=255)
                                   tCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGagc                                                                                                               >  2:3827682/1‑150 (MQ=255)
                                    ccAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGagca                                                                                                              <  2:9529637/150‑1 (MQ=255)
                                    ccAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGagca                                                                                                              >  2:6465205/1‑150 (MQ=255)
                                       gTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAgcg                                                                                                           <  1:6668453/150‑1 (MQ=255)
                                        ttGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAgcgc                                                                                                          <  1:5569681/150‑1 (MQ=255)
                                          gcagggcaggCTCGCCGAAGAAGGCCTCGGCGCCCTGCAGCACCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCTGAAGCGCCGGAGACCCGCGCCCGGTCCTCCCCGAGGCTCTCCGGATTGTCCTTCAGGTGTATGAGCAGCGcct                                                                                                        >  2:9234212/1‑150 (MQ=255)
                                             gggcaggCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGACCAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGccttca                                                                                                     >  2:1556144/1‑150 (MQ=255)
                                             gggcaggCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGccttca                                                                                                     <  1:7588007/150‑1 (MQ=255)
                                                caggCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGccttcaggt                                                                                                  <  2:8057032/150‑1 (MQ=255)
                                                 aggCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGccttcaggtc                                                                                                 >  1:3939628/1‑150 (MQ=255)
                                                 aggCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGccttcaggtc                                                                                                 <  2:2342791/150‑1 (MQ=255)
                                                 aggCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGccttcaggtc                                                                                                 >  2:7812455/1‑150 (MQ=255)
                                                   gCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGccttcaggtcct                                                                                               <  1:7303394/150‑1 (MQ=255)
                                                    cTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGccttcaggtcctt                                                                                              <  1:133105/150‑1 (MQ=255)
                                                     tCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGccttcaggtccttc                                                                                             <  1:7522741/150‑1 (MQ=255)
                                                      cGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGccttcaggtccttca                                                                                            >  2:10022648/1‑150 (MQ=255)
                                                      cGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGccttcaggtccttca                                                                                            <  2:4045306/150‑1 (MQ=255)
                                                        ccGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGccttcaggtccttcagg                                                                                          >  1:3309373/1‑150 (MQ=255)
                                                         cGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCNGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGccttcaggtccttcaggt                                                                                         >  1:5866572/1‑150 (MQ=255)
                                                            agaagGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGa                                                                                      >  1:8547172/1‑150 (MQ=255)
                                                             gaagGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGag                                                                                     <  2:10707312/150‑1 (MQ=255)
                                                             gaagGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGag                                                                                     >  2:4299600/1‑150 (MQ=255)
                                                                 gCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGagcagc                                                                                 <  2:3516407/150‑1 (MQ=255)
                                                                   cTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGCGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGagcagcag                                                                               <  2:6110792/150‑1 (MQ=255)
                                                                   cTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGagcagcag                                                                               >  1:1273579/1‑150 (MQ=255)
                                                                   cTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGagcagcag                                                                               >  2:7314339/1‑150 (MQ=255)
                                                                      ggCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGcagcagcag                                                                            <  2:2030981/150‑1 (MQ=255)
                                                                      ggCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGcagcagcag                                                                            >  2:2123528/1‑150 (MQ=255)
                                                                      ggCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGcagcagcag                                                                            >  1:294696/1‑150 (MQ=255)
                                                                      ggCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGcagcagcag                                                                            <  2:11088162/150‑1 (MQ=255)
                                                                       gcgcCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGGTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAgg                                                                           >  2:3058170/1‑150 (MQ=255)
                                                                         gcCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGcc                                                                         <  2:9614805/150‑1 (MQ=255)
                                                                          cccTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCTGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCt                                                                        >  1:10080429/1‑150 (MQ=255)
                                                                          cccTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCt                                                                        >  2:10494005/1‑150 (MQ=255)
                                                                          cccTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCt                                                                        >  1:7980886/1‑150 (MQ=255)
                                                                          cccTGCTGCTCCAGAGTGGACAGGCGGCGCAGCAGCGCCTGCCCGGACGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCATTCAGGTCCTTAAGGTCGAGCAGCAGCAGGCCt                                                                        >  2:5066361/1‑150 (MQ=255)
                                                                               ctgctCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCg                                                                   <  2:634727/150‑1 (MQ=255)
                                                                               ctgctCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCg                                                                   >  1:3664224/1‑150 (MQ=255)
                                                                                 gctCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGt                                                                 <  2:837716/150‑1 (MQ=255)
                                                                                   tCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCg                                                               >  1:7124300/1‑150 (MQ=255)
                                                                                      agagTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGcc                                                            <  1:11067369/150‑1 (MQ=255)
                                                                                          tGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCt                                                        >  2:2893978/1‑150 (MQ=255)
                                                                                           ggCCAGGCGGCGCAGCAGCCCCTGCCCGTGAGCGCCGGTGAACGGCGCCAGGTCCTCGCCGAGGTTCTCCGGCTTGTCGTTCTGGTGGTGGAGCAGCGACATCAGGGCCGTGAGGTCGAGAAGCTGCAGGCCTTCAAGGACGGCCagaag                                                       >  2:6712312/1‑146 (MQ=255)
                                                                                             ccAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGa                                                     <  2:9064205/150‑1 (MQ=255)
                                                                                              cAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGaa                                                    <  1:4035508/150‑1 (MQ=255)
                                                                                                    gcgcAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCCGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAggcggc                                              >  2:6255261/1‑150 (MQ=255)
                                                                                                      gcagcaGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGa                                            <  1:6487411/150‑1 (MQ=255)
                                                                                                       cagcagCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGAGCCTTCAGGTCCTTCAGCTCGAGCAGCATCAGGCCTACCCGGTCGGCCACCTGGAAGGCGGCGac                                           >  1:10486231/1‑149 (MQ=255)
                                                                                                        agcagcGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAAc                                          >  2:10656998/1‑150 (MQ=255)
                                                                                                         gcagcGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACa                                         >  1:3466526/1‑150 (MQ=255)
                                                                                                         gcagcGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACa                                         <  1:2295211/150‑1 (MQ=255)
                                                                                                           agcGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAgc                                       <  2:10294009/150‑1 (MQ=255)
                                                                                                           agcGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAgc                                       <  1:2417631/150‑1 (MQ=255)
                                                                                                            gcgcCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAgcg                                      <  1:9253704/150‑1 (MQ=255)
                                                                                                               ccTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAGCGCgg                                   >  1:6747276/1‑150 (MQ=255)
                                                                                                               ccTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCGCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCGTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAGCGCgg                                   >  2:4723199/1‑150 (MQ=255)
                                                                                                                 tGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAGCGCGGcc                                 <  1:8845289/150‑1 (MQ=255)
                                                                                                                     cGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAGCGCGGCctgct                             >  2:7653247/1‑150 (MQ=255)
                                                                                                                      ggAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAGCGCGGCctgctg                            <  1:5010427/150‑1 (MQ=255)
                                                                                                                       gAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAGCGCGGCCTGCTgg                           <  1:5390942/150‑1 (MQ=255)
                                                                                                                            gcCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAGCGCGGCCTGCTGGCTATc                      <  1:8163160/150‑1 (MQ=255)
                                                                                                                            gcCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAGCGCGGCCTGCTGGCTATc                      >  1:8292058/1‑150 (MQ=255)
                                                                                                                              cGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAGCGCGGCCTGCTGGCTATCgg                    <  2:7398119/150‑1 (MQ=255)
                                                                                                                                gTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAGCGCGGCCTGCTGGCTATCGGTc                  <  1:10344123/150‑1 (MQ=255)
                                                                                                                                gTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAGCGCGGCCTGCTGGCTATCGGTc                  >  2:2631319/1‑150 (MQ=255)
                                                                                                                                 tGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAGCGCGGCCTGCTGGCTATCGGTCa                 <  1:2884564/150‑1 (MQ=255)
                                                                                                                                   aaCAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAGCGCGGCCTGCTGGCTATCGGTCAAc               >  1:9847765/1‑150 (MQ=255)
                                                                                                                                   aaCAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAGCGCGGCCTGCTGGCTATCGGTCAAc               >  1:4293721/1‑150 (MQ=255)
                                                                                                                                   aaCAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAGCGCGGCCTGCTGGCTATCGGTCAAc               <  1:264258/150‑1 (MQ=255)
                                                                                                                                          cccGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAGCGCGGCCTGCTGGCTATCGGTCAACTCCagga        >  2:2770882/1‑150 (MQ=255)
                                                                                                                                          cccGGACCTCGCCTGGGATCTCCGGATTGTCCTTCAGGAGGTTGAGCAGCGCCTTCATGTCCTGCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGACACAAGGAATCCTGCGACCAGCGCGCCCTGCTGGCTATCGGTCAACTCCagga        >  2:5181971/1‑150 (MQ=255)
                                                                                                                                           ccGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAGCGCGGCCTGCTGGCTATCGGTCAACTCCaggag       <  1:94440/150‑1 (MQ=255)
                                                                                                                                            cGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAGCGCGGCCTGCTGGCTATCGGTCAACTCCaggagg      <  1:2234823/150‑1 (MQ=255)
                                                                                                                                              gTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAGCGCGGCCTGCTGGCTATCGGTCAACTCCAGGAGGtt    >  2:8827112/1‑150 (MQ=255)
                                                                                                                                              gTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAGCGCGGCCTGCTGGCTATCGGTCAACTCCAGGAGGtt    <  1:5075616/150‑1 (MQ=255)
                                                                                                                                               tCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAGCGCGGCCTGCTGGCTATCGGTCAACTCCAGGAGGTTg   <  1:8931156/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GCCCCCGCCCGTCCGGCTCGGGGCGGAGGATGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTTGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAGCGCGGCCTGCTGGCTATCGGTCAACTCCAGGAGGTTGG  >  NC_008463/4997639‑4997932

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑