Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 732,660 G→A intergenic (+114/‑426) tyrS → / → PA14_RS03450 tyrosine‑‑tRNA ligase/16S ribosomal RNA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_008463732,6600GA99.0% 361.5 / NA 98intergenic (+114/‑426)tyrS/PA14_RS03450tyrosine‑‑tRNA ligase/16S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (73/24);  minor base T (0/1);  total (73/25)
Fisher's exact test for biased strand distribution p-value = 2.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 105 total aligned reads displayed.

GGCTTTCGCGCGCATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGGGTGGTGAAGCGCTCCGGCAGGTTGTTCGCCGCAG  >  NC_008463/732513‑732694
                                                                                                                                                   |                                  
ggCTTTCGCGCGCATCACGCTGAACGCTGAATAATTCTTTCATATAAGTGGTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGACGAGTAAATGCTTGAAAATCAAGGAGTTCCGGATTCTCTCCCAGGagt                                  >  2:240640/1‑150 (MQ=17)
  cTTTCGCGCGCATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGagtgg                                >  1:10219672/1‑150 (MQ=255)
  cTTTCGCGCGCATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGagtgg                                >  2:884627/1‑150 (MQ=255)
   tttCGCGCGCATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCGCAGGagtggt                               >  1:7350400/1‑150 (MQ=255)
   tttCGCGCGCATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGagtggt                               >  2:3991360/1‑150 (MQ=255)
   tttCGCGCGCATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGagtggt                               >  1:7350394/1‑150 (MQ=255)
   tttCGCGCGCATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGagtggt                               <  1:5472669/150‑1 (MQ=255)
    ttCGCGCGCATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAgtggtg                              <  1:9794923/150‑1 (MQ=255)
    ttCGCGCGCATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAgtggtg                              >  2:2696508/1‑150 (MQ=255)
    ttCGCGCGCATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAgtggtg                              >  1:8356162/1‑150 (MQ=255)
    ttCGCGCGCATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAgtggtg                              <  1:7068328/150‑1 (MQ=255)
    ttCGCGCGCATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAgtggtg                              <  1:6701344/150‑1 (MQ=255)
    ttCGCGCGCATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAgtggtg                              >  1:4775182/1‑150 (MQ=255)
     tCGCGCGCATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGa                             <  2:4210807/150‑1 (MQ=255)
     tCGCGCGCATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGa                             >  2:3464828/1‑150 (MQ=255)
     tCGCGCGCATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGa                             <  2:3322771/150‑1 (MQ=255)
     tCGCGCGCATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGa                             <  2:6165689/150‑1 (MQ=255)
     tCGCGCGCATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGa                             >  1:10138645/1‑150 (MQ=255)
      cgcgcgCATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGaa                            <  2:8269122/150‑1 (MQ=255)
      cgcgcgCATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGAAAATGCTTGAAAATCAAGGATATACGGATTCTCTCCAAGGAGTGGTGaa                            >  1:1692294/1‑150 (MQ=255)
      cgcgagCATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGaa                            >  1:8835742/1‑150 (MQ=255)
       gcgcgcATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGacg                           >  2:8816450/1‑148 (MQ=255)
       gcgcgcATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAg                           <  2:1605821/150‑1 (MQ=255)
       gcgcgcATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAg                           <  1:10618673/150‑1 (MQ=255)
       gcgcgcATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAg                           <  2:4429005/150‑1 (MQ=255)
       gcgcgcATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAg                           <  2:140865/150‑1 (MQ=255)
       gcgcgcATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAg                           <  1:10618713/150‑1 (MQ=255)
       gcgcgcATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAg                           >  1:5016492/1‑150 (MQ=255)
        cgcgcATCACGCTGAAAGCTGACTAATTCTTAAAAATAAGAGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCGCAGGAGTGGTGAAgc                          >  2:10826549/1‑150 (MQ=14)
         gcgcATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAgcg                         <  2:586270/150‑1 (MQ=255)
         gcgcATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAgcg                         >  2:8674936/1‑150 (MQ=255)
         gcgcATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAgcg                         >  2:10142895/1‑150 (MQ=255)
         gcgcATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAgcg                         >  1:3268718/1‑150 (MQ=255)
         gcgcATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAgcg                         >  1:5721824/1‑150 (MQ=255)
          cgcATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAgcgc                        >  1:11073710/1‑150 (MQ=255)
           gcATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCt                       >  2:5229830/1‑150 (MQ=255)
           gcATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCt                       >  1:9655066/1‑150 (MQ=255)
           gcATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCt                       >  1:9654967/1‑150 (MQ=255)
           gcATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTAGAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCt                       >  1:7582150/1‑150 (MQ=255)
            cATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTc                      >  2:10941595/1‑150 (MQ=255)
            cATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTc                      >  1:4085917/1‑150 (MQ=255)
             aTCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTcc                     >  1:10539064/1‑150 (MQ=255)
             aTCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTcc                     <  1:3296211/150‑1 (MQ=255)
             aTCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTcc                     >  1:3031110/1‑150 (MQ=255)
             aTCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCGATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTcc                     >  2:8790245/1‑150 (MQ=255)
              tCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCg                    <  1:7831547/150‑1 (MQ=255)
              tCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCg                    >  2:10183918/1‑150 (MQ=255)
              tCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCg                    >  2:2335768/1‑150 (MQ=255)
               cACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCgg                   >  2:1813795/1‑150 (MQ=255)
                aCGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGc                  >  1:9711108/1‑150 (MQ=255)
                 cGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCa                 >  2:343056/1‑150 (MQ=255)
                  gCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAg                <  2:7675514/150‑1 (MQ=255)
                  gCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAg                >  1:9596265/1‑150 (MQ=255)
                   cTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTAGAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGAGTTACGGATTCTCTCCCCGGAGTGGTGAAGCGCTCCGGCAgg               <  2:10189746/150‑1 (MQ=255)
                    tGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGTGTGGTGAAGCGCTCCGGCAGgt              <  1:1544697/150‑1 (MQ=255)
                    tGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGgt              <  1:1544459/150‑1 (MQ=255)
                    tGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGgt              >  1:9041834/1‑150 (MQ=255)
                    tGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGgt              >  1:5721235/1‑150 (MQ=255)
                    tGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGgt              >  2:8336466/1‑150 (MQ=255)
                     gAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGgtt             >  2:2317381/1‑150 (MQ=255)
                     gAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGgtt             >  2:10859054/1‑150 (MQ=255)
                     gAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGgtt             >  2:10859053/1‑150 (MQ=255)
                     gAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGgtt             <  2:10792708/150‑1 (MQ=255)
                     gAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGgtt             <  2:10790213/150‑1 (MQ=255)
                     gAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGgtt             >  2:10104532/1‑150 (MQ=255)
                     gAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGgtt             >  2:6469685/1‑150 (MQ=255)
                     gAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGgtt             <  1:7402227/150‑1 (MQ=255)
                      aaaGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGgttg            >  1:2778582/1‑150 (MQ=255)
                      aaaGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGgttg            >  1:6653678/1‑150 (MQ=255)
                      aaaGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGgttg            >  1:6653738/1‑150 (MQ=255)
                      aaaGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGgttg            >  1:6653749/1‑150 (MQ=255)
                       aaGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGgttgt           >  2:3373089/1‑150 (MQ=38)
                       aaGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGgttgt           >  1:932881/1‑150 (MQ=38)
                       aaGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGgttgt           >  2:8740367/1‑150 (MQ=38)
                       aaGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGgttgt           >  1:6718946/1‑150 (MQ=38)
                        aGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAACTGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGgttgtt          <  2:6574069/150‑1 (MQ=255)
                         gCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGGTTGTTc         <  1:9759347/150‑1 (MQ=37)
                          cTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGGTTGTTcg        >  2:2760267/1‑150 (MQ=25)
                           tGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGGTTGTTcgc       >  1:3936668/1‑150 (MQ=25)
                           tGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGGTTGTTcgc       >  2:4195721/1‑150 (MQ=25)
                           tGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGGTTGTTcgc       >  2:4981708/1‑150 (MQ=25)
                           tGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGGTTGTTcgc       >  2:4879403/1‑150 (MQ=25)
                            gAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGGTTGTTcgcc      >  1:1154320/1‑150 (MQ=21)
                            gAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGGTTGTTcgcc      >  2:11008004/1‑150 (MQ=21)
                             aataatTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGGTTGTTcgccg     >  2:4752926/1‑150 (MQ=18)
                             aataatTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGGTTGTTcgccg     >  2:4752753/1‑150 (MQ=18)
                             aataatTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGGTTGTTcgccg     >  1:4778661/1‑150 (MQ=18)
                             aataatTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGGTTGTTcgccg     >  1:1906357/1‑150 (MQ=18)
                              ataatTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGGTTGTTcgccgc    >  2:1180601/1‑150 (MQ=17)
                              ataatTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGGTTGTTcgccgc    >  1:2162800/1‑150 (MQ=17)
                              ataatTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGGTTGTTcgccgc    >  1:222206/1‑150 (MQ=17)
                              ataatTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGGTTGTTcgccgc    >  1:6709779/1‑150 (MQ=17)
                              ataatTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGGTTGTTcgccgc    >  2:3333330/1‑150 (MQ=17)
                              ataatTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCACCATATAATGCTCACCACTCCCTGCGAAGACTAAGAGTAAATGATTGAATTTCAAGGATTTACGGATTCTCTCAAAGGAGTGGTGAAGCGCTCCGGCAGGTTGTTcgcagc    >  2:1788582/1‑150 (MQ=11)
                               taatTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGGTTGTTCGCCGCa   >  1:5889438/1‑150 (MQ=17)
                               taatTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGGTTGTTCGCCGCa   >  1:1885675/1‑150 (MQ=17)
                               taatTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGGTTGTTCGCCGCa   >  1:9232158/1‑150 (MQ=17)
                               taatTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGGTTGTTCGCCGCa   >  1:9351860/1‑150 (MQ=17)
                                aatTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGGTTGTTCGCCGCAg  >  2:824740/1‑150 (MQ=14)
                                aatTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGGTTGTTCGCCGCAg  >  2:9094309/1‑150 (MQ=14)
                                                                                                                                                   |                                  
GGCTTTCGCGCGCATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGGGTGGTGAAGCGCTCCGGCAGGTTGTTCGCCGCAG  >  NC_008463/732513‑732694

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑