Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 6,076,066 G→C R105R (CGG→CGC)  PA14_RS27775 → methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084636,076,0660GC99.8% 2032.0 / NA 531R105R (CGG→CGC) PA14_RS27775methyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base C (260/270);  minor base A (0/1);  total (260/271)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 531 total aligned reads displayed.

GCGCAGCCTCGAACAGGCCGACGCGGAGCACAACCAGCCCACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGGTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCTGGGCCGCCTGCTGGCCCATGCGGGGCAACC  >  NC_008463/6075919‑6076212
                                                                                                                                                   |                                                                                                                                                  
   cAGCCTCGAACAGGCCGACGCGGAGCACAACCAGCCCACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCaa                                                                                                                                               >  1:11066977/1‑150 (MQ=255)
    aGCCTCGAACAGGCCGACGCGGAGCACAACCAGCCCACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAAt                                                                                                                                              >  1:3190053/1‑150 (MQ=255)
     gCCTCGAACAGGCCGACGCGGAGCACAACCAGCCCACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGCACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATg                                                                                                                                             >  1:8546263/1‑150 (MQ=255)
      ccTCGAACAGGCCGACGCGGAGCACAACCAGCCCACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGt                                                                                                                                            <  1:10921896/150‑1 (MQ=255)
       cTCGAACAGGCCGACGCGGAGCACAACCAGCCCACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGTCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTc                                                                                                                                           <  1:7138294/150‑1 (MQ=255)
         cGAACAGGCCGACGCGGAGCACAACCAGCCCACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCCTTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGa                                                                                                                                         <  2:4824355/150‑1 (MQ=255)
         cGAACAGGCCGACGCGGAGCACAACCAGCCCACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGa                                                                                                                                         <  1:8405850/150‑1 (MQ=255)
         cGAACAGGCCGACGCGGAGCACAACCAGCCCACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGa                                                                                                                                         <  2:16155/150‑1 (MQ=255)
         cGAACAGGCCGACGCGGAGCACAACCAGCCCACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGa                                                                                                                                         >  2:8721704/1‑150 (MQ=255)
          gAACAGGCCGACGCGGAGCACAACCAGCCCACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCAGTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGAc                                                                                                                                        >  2:10237941/1‑150 (MQ=255)
                      gcgGAGCACAACCAGCCCACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGc                                                                                                                            <  2:1894274/150‑1 (MQ=255)
                      gcgGAGCACAACCAGCCCACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATGGGCGGAACGCCCGCAGGCACCGCACCGCTTCAATGTCGACGTACCGGGACGc                                                                                                                            >  2:554275/1‑150 (MQ=255)
                       cgGAGCACAACCAGCCCACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCa                                                                                                                           >  2:9651973/1‑150 (MQ=255)
                        ggAGCACAACCAGCCCACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCac                                                                                                                          >  1:2903992/1‑149 (MQ=255)
                               aaCCAGCCCACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGgcag                                                                                                                   <  1:10795930/150‑1 (MQ=255)
                                aCCAGCCCACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCTAGTGgcagc                                                                                                                  <  2:2459027/150‑1 (MQ=255)
                                aCCAGCCCACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGgcagc                                                                                                                  <  2:3337877/150‑1 (MQ=255)
                                 ccAGCCCACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGACCTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGgctgca                                                                                                                 >  2:5960640/1‑150 (MQ=255)
                                   aGCCCACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTAACGGGACGCAAGTGGCAGCAGa                                                                                                               >  2:2116623/1‑150 (MQ=255)
                                    gCCCACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGAt                                                                                                              <  1:7819747/150‑1 (MQ=255)
                                     cccACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATc                                                                                                             <  1:7556139/150‑1 (MQ=255)
                                     cccACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATc                                                                                                             <  2:5013864/150‑1 (MQ=255)
                                     cccACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATc                                                                                                             <  2:10457163/150‑1 (MQ=255)
                                     cccACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATc                                                                                                             <  1:5185098/150‑1 (MQ=255)
                                      ccACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGCACGCCCGCAGGCACCGACCCGCTTCAATGTCGACGTACCGGGACGCAAGGGGCAGCAGATCg                                                                                                            >  2:6356220/1‑150 (MQ=255)
                                      ccACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCg                                                                                                            >  2:7377450/1‑150 (MQ=255)
                                          ccGCCTGGCCGCGCCCGCGCCTTGTCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCt                                                                                                        <  2:8443949/150‑1 (MQ=255)
                                          ccGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCt                                                                                                        <  1:2257423/150‑1 (MQ=255)
                                            gCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGt                                                                                                      >  2:2406149/1‑150 (MQ=255)
                                             ccTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGTCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGtt                                                                                                     <  2:5762515/150‑1 (MQ=255)
                                             ccTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGtt                                                                                                     <  1:6188975/150‑1 (MQ=255)
                                              cTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTc                                                                                                    <  1:9413215/150‑1 (MQ=255)
                                                ggCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGTTCGGCCTGTTCGc                                                                                                  >  1:9511390/1‑150 (MQ=255)
                                                 gCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGcc                                                                                                 <  2:6068817/150‑1 (MQ=255)
                                                    gcgcccgcgccTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCggg                                                                                              >  1:1008888/1‑150 (MQ=255)
                                                    gcgcccgcgccTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCggg                                                                                              <  1:10682050/150‑1 (MQ=255)
                                                     cgcccgcgccTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGCCGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGgc                                                                                             >  2:4484594/1‑150 (MQ=255)
                                                        ccgcgccTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGcc                                                                                          >  2:6633554/1‑150 (MQ=255)
                                                             ccTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAg                                                                                     >  2:6790423/1‑150 (MQ=255)
                                                                tATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGACCTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTc                                                                                  >  1:6979037/1‑150 (MQ=255)
                                                                  tCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCACTGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGc                                                                                <  1:4147897/150‑1 (MQ=255)
                                                                      cAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCccgg                                                                            >  1:46449/1‑150 (MQ=255)
                                                                      cAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCccgg                                                                            <  2:897835/150‑1 (MQ=255)
                                                                       aGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCccggc                                                                           >  2:3883383/1‑150 (MQ=255)
                                                                          tCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGGTCGCccggccgg                                                                        >  2:3057696/1‑150 (MQ=255)
                                                                           cgccgcCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGgt                                                                       <  2:9721902/150‑1 (MQ=255)
                                                                           cgccgcCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGgt                                                                       <  1:440098/150‑1 (MQ=255)
                                                                           cgccgcCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGgt                                                                       >  1:3451029/1‑150 (MQ=255)
                                                                             ccgccCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGACATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGgtcg                                                                     <  1:4444366/150‑1 (MQ=255)
                                                                               gccCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGgtcgtc                                                                   <  1:6504700/150‑1 (MQ=255)
                                                                                cccAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTcc                                                                  >  1:7165678/1‑150 (MQ=255)
                                                                                cccAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTcc                                                                  >  2:7129104/1‑150 (MQ=255)
                                                                                  cAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCAc                                                                >  1:9207933/1‑150 (MQ=255)
                                                                                   aGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACt                                                               >  2:10652664/1‑150 (MQ=255)
                                                                                   aGGCCGCAGCGCTGGGCGCGGTCGGCGAACAGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACt                                                               <  2:2187286/150‑1 (MQ=255)
                                                                                     gCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTgg                                                             >  2:7789045/1‑150 (MQ=255)
                                                                                       cGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCt                                                           >  1:396920/1‑150 (MQ=255)
                                                                                         cAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGcgcg                                                         >  2:8243584/1‑150 (MQ=255)
                                                                                          aGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGACCCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGa                                                        <  2:854321/150‑1 (MQ=255)
                                                                                          aGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGa                                                        <  1:7725050/150‑1 (MQ=255)
                                                                                             gcTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTg                                                     <  2:2615219/150‑1 (MQ=255)
                                                                                                 ggCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGc                                                 >  1:3583183/1‑150 (MQ=255)
                                                                                                    gcgGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTcc                                              >  1:166783/1‑150 (MQ=255)
                                                                                                    gcgGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTcc                                              <  1:8308352/150‑1 (MQ=255)
                                                                                                    gcgGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGACCTGCCGGTCGTCCACAGGCGCGACGGGTGCTcg                                              >  2:3162337/1‑149 (MQ=255)
                                                                                                     cgGTCGGCGAACTGCCATGGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCg                                             <  1:10247705/150‑1 (MQ=255)
                                                                                                      ggTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCgg                                            >  1:6304279/1‑150 (MQ=255)
                                                                                                        tCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCa                                          >  1:4292258/1‑150 (MQ=255)
                                                                                                        tCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCa                                          <  2:9104034/150‑1 (MQ=255)
                                                                                                        tCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCa                                          >  1:9891686/1‑150 (MQ=255)
                                                                                                         cGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCCGTTCGCCCGGCCGGTCGTCGTCTGGCTCGCCTGGTGCTCAGGCaa                                         >  2:10943062/1‑150 (MQ=255)
                                                                                                         cGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCaa                                         <  1:10654891/150‑1 (MQ=255)
                                                                                                         cGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCaa                                         <  1:8142660/150‑1 (MQ=255)
                                                                                                           gCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAgg                                       >  2:8036587/1‑150 (MQ=255)
                                                                                                            cGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGGCCACTGGCTCGACTGGTGCTCCGGCAAggg                                      >  2:5337301/1‑150 (MQ=255)
                                                                                                                cTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCAt                                  >  2:366337/1‑150 (MQ=255)
                                                                                                                  gCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCt                                <  2:418480/150‑1 (MQ=255)
                                                                                                                  gCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCt                                <  1:5713975/150‑1 (MQ=255)
                                                                                                                  gCCAGTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCACGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCt                                <  2:2889510/150‑1 (MQ=255)
                                                                                                                      ttGGCGGAACGCCCGCAGGCACCGAACCGCTTCACTGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCTGGgc                            <  1:48816/150‑1 (MQ=255)
                                                                                                                          cGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCTGGgccgcc                        <  1:9680676/150‑1 (MQ=255)
                                                                                                                            gAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCTGGGCCGCctg                      >  1:1212094/1‑150 (MQ=255)
                                                                                                                                 cccGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCTGGGCCGCCTGCTGGc                 >  1:5447560/1‑150 (MQ=255)
                                                                                                                                  ccGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCTGGGCCGCCTGCTGGcc                >  1:2422495/1‑150 (MQ=255)
                                                                                                                                   cGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCTGGGCCGCCTGCTGGccc               >  2:9480866/1‑150 (MQ=255)
                                                                                                                                    gCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCTGGGCCGCCTGCTGGCCCa              <  1:3033670/150‑1 (MQ=255)
                                                                                                                                    gCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCGGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCTGGGCCGCCTGCTGGCCCa              <  1:886314/150‑1 (MQ=255)
                                                                                                                                      aGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCTGGGCCGCCTGCTGGCCCATg            >  1:592035/1‑150 (MQ=255)
                                                                                                                                      aGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCTGGGCCGCCTGCTGGCCCATg            >  2:6161820/1‑150 (MQ=255)
                                                                                                                                       ggCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCTGGGCCGCCTGCTGGCCCATGc           <  1:1397936/150‑1 (MQ=255)
                                                                                                                                       ggCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCTGGGCCGCCTGCTGGCCCATGc           <  2:2255394/150‑1 (MQ=255)
                                                                                                                                        gCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCTGGGCCGCCTGCTGGCCCATGCg          <  1:2646711/150‑1 (MQ=255)
                                                                                                                                        gCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCTGGGCCGCCTGCTGGCCCATGCg          <  1:6637599/150‑1 (MQ=255)
                                                                                                                                        gCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCTGGGCCGCCTGCTGGCCCATGCg          <  2:1468857/150‑1 (MQ=255)
                                                                                                                                          aCCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGGTCGACTGGTGCTCCGGCAAGGGCCATCTGGGCCGCCTGCTGGCCCTTGCggg        >  2:7533/1‑150 (MQ=255)
                                                                                                                                              aaCCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCTGGGCCGCCTGCTGGCCCATGCGGGGCaa    >  1:6041634/1‑150 (MQ=255)
                                                                                                                                              aaCCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCTGGGCCGCCTGCTGGCCCATGCGGGGCaa    >  1:201211/1‑150 (MQ=255)
                                                                                                                                              aaCCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCTGGGCCGCCTGCTGGCCCATGCGGGGCaa    <  1:8416196/150‑1 (MQ=255)
                                                                                                                                               aCCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCTGGGCCGCCTGCTGGCCCATGCGGGGCAAc   <  2:6981921/150‑1 (MQ=255)
                                                                                                                                                ccGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCTGGGCCGCCTGCTGGCCCATGCGGGGCAAcc  >  2:6472121/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GCGCAGCCTCGAACAGGCCGACGCGGAGCACAACCAGCCCACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGGTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCTGGGCCGCCTGCTGGCCCATGCGGGGCAACC  >  NC_008463/6075919‑6076212

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑