Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 927,917 T→C I56T (ATC→ACC)  PA14_RS04340 → PAS domain S‑box protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_008463927,9170TC99.8% 2439.3 / NA 616I56T (ATC→ACC) PA14_RS04340PAS domain S‑box protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (265/350);  minor base G (1/0);  total (266/350)
Fisher's exact test for biased strand distribution p-value = 4.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 618 total aligned reads displayed.

AGTTCAGGCTGAGCCGCTGGGCGGGCGCGACACTGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGATCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCCGCGACATCGACCGCGAGTTTCCCGGCGCCCGCGGCT  >  NC_008463/927770‑928063
                                                                                                                                                   |                                                                                                                                                  
aGTTCAGGCTGAGCCGCTGGGCGGGCGCGACACTGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGAccc                                                                                                                                                  >  1:9331976/1‑150 (MQ=255)
   tCAGGCTGAGCCGCTGGGCGGGCGCGACACTGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCACCAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACccgcc                                                                                                                                               >  1:9691587/1‑150 (MQ=255)
   tCAGGCTGAGCCGCTGGGCGGGCGCGACACTGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACccgcc                                                                                                                                               <  1:1173426/150‑1 (MQ=255)
    cAGGCTGAGCCGCTGGGCGGGCGCGACACTGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACccgccg                                                                                                                                              >  1:5691513/1‑150 (MQ=255)
      ggCTGAGCCGCTGGGCGGGCGCGACACTGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCcgcg                                                                                                                                            >  2:7179572/1‑150 (MQ=255)
      ggCTGAGCCGCTGGGCGGGCGCGACACTGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCcgcg                                                                                                                                            >  1:9426302/1‑150 (MQ=255)
         tGAGCCGCTGGGCGGGCGCGACACTGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTgg                                                                                                                                         <  1:6151430/150‑1 (MQ=255)
         tGAGCCGCTGGGCGGGCGCGACACTGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTgg                                                                                                                                         <  1:5067313/150‑1 (MQ=255)
           aGCCGCTGGGCGGGCGCGACACTGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGaa                                                                                                                                       >  1:7484643/1‑150 (MQ=255)
               gCTGGGCGGGCGCGACACTGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAActct                                                                                                                                   <  1:6322297/150‑1 (MQ=255)
               gCTGGGCGGGCGCGACACTGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAActct                                                                                                                                   <  2:3040322/150‑1 (MQ=255)
                      gggcgCGACACTGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTa                                                                                                                            <  2:10924193/150‑1 (MQ=255)
                      gggcgCGACACTGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTa                                                                                                                            >  1:3407504/1‑150 (MQ=255)
                      gggcgCGACACTGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTa                                                                                                                            <  1:3105890/150‑1 (MQ=255)
                       ggcgCGACACTGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTAt                                                                                                                           <  2:5843612/150‑1 (MQ=255)
                       ggcgCGACACTGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTAt                                                                                                                           >  2:2760264/1‑150 (MQ=255)
                          gcgACACTGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGc                                                                                                                        >  1:2571323/1‑150 (MQ=255)
                           cgACACTGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCt                                                                                                                       >  2:9083687/1‑150 (MQ=255)
                            gACACTGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCtt                                                                                                                      <  1:8511709/150‑1 (MQ=255)
                             acacTGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTg                                                                                                                     <  1:2759460/150‑1 (MQ=255)
                             acacTGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTg                                                                                                                     >  2:7939208/1‑150 (MQ=255)
                             acacTGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTg                                                                                                                     >  1:1372076/1‑150 (MQ=255)
                             acacTGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTg                                                                                                                     >  2:4875424/1‑150 (MQ=255)
                                cTGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGt                                                                                                                  >  2:11102498/1‑150 (MQ=255)
                                     gcTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGt                                                                                                             >  2:89516/1‑150 (MQ=255)
                                          gagtGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCGGGTCCACGAGCAACTGGCGCGGAGGGCGCGGGAACGCGAGGCGGACCTGCGGGGCGCGGCGGGGCCGCGCCGCGTGGAAGTCTTCCGGTCTGGCGTGGGGGGGGGGcgcg                                                                                                        >  2:10303359/4‑150 (MQ=255)
                                          gACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGcgcg                                                                                                        >  1:5986923/1‑150 (MQ=255)
                                          gACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGcgcg                                                                                                        >  2:6639031/1‑150 (MQ=255)
                                            cTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGt                                                                                                      <  1:10021258/150‑1 (MQ=255)
                                               ttGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGcc                                                                                                   <  2:8828733/150‑1 (MQ=255)
                                               ttGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGcc                                                                                                   <  2:351684/150‑1 (MQ=255)
                                                tGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCt                                                                                                  <  2:6060302/150‑1 (MQ=255)
                                                 gCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCtt                                                                                                 <  2:532417/150‑1 (MQ=255)
                                                 gCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCtt                                                                                                 <  2:9489153/150‑1 (MQ=255)
                                                  cTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTg                                                                                                <  1:2022983/150‑1 (MQ=255)
                                                    gTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCt                                                                                              <  2:3603048/150‑1 (MQ=255)
                                                      ccGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGa                                                                                            <  1:6495063/150‑1 (MQ=255)
                                                        gggCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACa                                                                                          <  1:5797665/150‑1 (MQ=255)
                                                          gCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGc                                                                                        <  2:1787204/150‑1 (MQ=255)
                                                          gCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGc                                                                                        <  2:9378543/150‑1 (MQ=255)
                                                                 gcCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAg                                                                                 <  2:7560106/150‑1 (MQ=255)
                                                                 gcCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAg                                                                                 <  1:8713040/150‑1 (MQ=255)
                                                                  ccTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAgg                                                                                >  1:10777960/1‑150 (MQ=255)
                                                                  ccTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAgg                                                                                >  2:2550652/1‑150 (MQ=255)
                                                                   cTGGGGGCTGGCGCTGGTCACCGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCGTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGgc                                                                               <  2:2057847/150‑1 (MQ=255)
                                                                    tGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGgcg                                                                              >  1:10194506/1‑150 (MQ=255)
                                                                     gggggCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGgcgc                                                                             >  1:8922935/1‑150 (MQ=255)
                                                                      ggggCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGca                                                                            <  2:10376704/150‑1 (MQ=255)
                                                                      ggggCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGca                                                                            >  2:2659272/1‑150 (MQ=255)
                                                                       gggCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGcac                                                                           <  2:8116858/150‑1 (MQ=255)
                                                                          cTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATc                                                                        >  1:7706008/1‑150 (MQ=255)
                                                                            ggCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGa                                                                      >  1:8107148/1‑150 (MQ=255)
                                                                            ggCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGa                                                                      >  1:5511601/1‑150 (MQ=255)
                                                                              cgcTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGAcc                                                                    <  1:9845477/150‑1 (MQ=255)
                                                                              cgcTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGAcc                                                                    <  2:8390948/150‑1 (MQ=255)
                                                                                cTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACcgc                                                                  <  1:10496970/150‑1 (MQ=255)
                                                                                cTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACcgc                                                                  >  2:5727894/1‑150 (MQ=255)
                                                                                 tGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACcgcg                                                                 <  2:1490341/150‑1 (MQ=255)
                                                                                         gAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTc                                                         <  2:4316179/150‑1 (MQ=255)
                                                                                         gAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTc                                                         <  2:2299640/150‑1 (MQ=255)
                                                                                            cAACAGGCGCGGATGGCGCTGGACCGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTccgc                                                      <  1:2436983/150‑1 (MQ=255)
                                                                                             aaCAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTccgcc                                                     <  2:159582/150‑1 (MQ=255)
                                                                                             aaCAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTccgcc                                                     >  2:6245247/1‑150 (MQ=255)
                                                                                              aCAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTccgccg                                                    >  1:1593260/1‑150 (MQ=255)
                                                                                                    gcgGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGACCTGTTCCGCCGCTACAg                                              >  2:4140550/1‑150 (MQ=255)
                                                                                                     cgGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGc                                             >  1:11142248/1‑150 (MQ=255)
                                                                                                      ggATGGCGCTGGACCGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGcc                                            <  1:6828293/150‑1 (MQ=255)
                                                                                                      ggATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGACCTGTTCCGCCGCTACAGcc                                            >  2:6937871/1‑150 (MQ=255)
                                                                                                      ggATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGcc                                            >  2:10535039/1‑150 (MQ=255)
                                                                                                         tGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGa                                         <  2:5180506/150‑1 (MQ=255)
                                                                                                           gcgcTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGAcc                                       >  2:1154194/1‑150 (MQ=255)
                                                                                                           gcgcTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGAGACCCGCCGCGTGGAACTCTACGAGTATGGCTTGCGTGGGGGGCGCGGTGCCTTGCTGACAGCGGGCGACGCGCACATCGACCGCGAACTGTGCCGCCGCTCCAGCCTGAcc                                       >  2:7645740/1‑150 (MQ=255)
                                                                                                             gcTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCcg                                     <  1:11039225/150‑1 (MQ=255)
                                                                                                                 gAACGCGAGGCGGAACTGCGGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCCGCGAc                                 <  1:7811531/150‑1 (MQ=255)
                                                                                                                   aCGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCCGCGACAt                               <  1:3715632/150‑1 (MQ=255)
                                                                                                                       gAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCCGCGACATCGAc                           <  1:4259806/150‑1 (MQ=255)
                                                                                                                        aGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCCGCGACATCGAcc                          >  2:4492767/1‑150 (MQ=255)
                                                                                                                         ggCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCCGCGACATCGACcg                         >  1:4345387/1‑150 (MQ=255)
                                                                                                                          gCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCCGCGACATCGACcgc                        >  1:10656935/1‑150 (MQ=255)
                                                                                                                            ggAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCCGCGACATCGACCGCGa                      <  1:4831185/150‑1 (MQ=255)
                                                                                                                             gAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGCCAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCCGCGACATCGACCGCGAg                     <  2:6499746/150‑1 (MQ=255)
                                                                                                                               aCTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCCGCGACATCGACCGCGAGtt                   <  2:3385516/150‑1 (MQ=255)
                                                                                                                                ctgctgGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCCGCGACATCGACCGCGAGttt                  <  2:9592310/150‑1 (MQ=255)
                                                                                                                                  gctgGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCCGCGACATCGACCGCGAGTTTcc                >  1:4725905/1‑150 (MQ=255)
                                                                                                                                  gctgGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCCGCGACATCGACCGCGAGTTTcc                >  1:9617378/1‑150 (MQ=255)
                                                                                                                                   ctgGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCCGCGACATCGACCGCGAGTTTccc               <  2:10065464/150‑1 (MQ=255)
                                                                                                                                   ctgGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCCGCGACATCGACCGCGAGTTTccc               <  2:6779881/150‑1 (MQ=255)
                                                                                                                                   ctgGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCCGCGACATCGACCGCGAGTTTccc               >  2:4746898/1‑150 (MQ=255)
                                                                                                                                     ggCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCCGCGACATCGACCGCGAGTTTCCCgg             >  2:3945030/1‑150 (MQ=255)
                                                                                                                                      gCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCCGCGACATCGACCGCGAGTTTCCCGgc            <  1:7103539/150‑1 (MQ=255)
                                                                                                                                        cGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCCGCGACATCGACCGCGAGTTTCCCGgcgc          >  1:2968676/1‑150 (MQ=255)
                                                                                                                                         gAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCCGCGACCTCGACCGCGAGTTTCCCGGCGcc         >  2:9737819/1‑150 (MQ=255)
                                                                                                                                          aGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCCGCGACATCGACCGCGAGTTTCCCGGCGccc        >  1:4009393/1‑150 (MQ=255)
                                                                                                                                          aGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCCGCGACATCGACCGCGAGTTTCCCGGCGccc        >  2:9847257/1‑150 (MQ=255)
                                                                                                                                           ggCGGTGACCCGCCGCGTGGAACTCTACCCGTATGGCTTGCGTGGGGGGCGCGGTGCCTTGCTGACTGCGGGCGAGGCGCAACTCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCCGCGACATCGACCGCGAGTTTCCCGGCGCCcg       <  1:8373892/150‑1 (MQ=255)
                                                                                                                                            gCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCCGCGACATCGACCGCGAGTTTCCCGGCGCCcgc      >  2:10363922/1‑150 (MQ=255)
                                                                                                                                            gCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCCGCGACATCGACCGCGAGTTTCCCGGCGCCcgc      >  1:2217457/1‑150 (MQ=255)
                                                                                                                                            gCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCCGCGACATCGACCGCGAGTTTCCCGGCGCCcgc      >  1:1761761/1‑150 (MQ=255)
                                                                                                                                            gCGGTGACCCGCCGCGTGGAACACTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCCGCGACATCGACCGCGAGTTTCCCGGCGCCcgc      >  1:5298577/1‑150 (MQ=255)
                                                                                                                                               gTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCCGCGACATCGACCGCGAGTTTCCCGGCGCCCGcggc   >  1:4604150/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
AGTTCAGGCTGAGCCGCTGGGCGGGCGCGACACTGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGATCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCCGCGACATCGACCGCGAGTTTCCCGGCGCCCGCGGCT  >  NC_008463/927770‑928063

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑