Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 1,356,548 G→C R69G (CGT→GGT)  PA14_RS06375 ← MFS transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084631,356,5480GC100.0% 2009.6 / NA 514R69G (CGT→GGT) PA14_RS06375MFS transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (218/296);  total (218/296)

Only 100 of 514 total aligned reads displayed.

TGCTGTCGTAGCCGGGGAGGACGCCGATCAGGGTGGTGGATACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACGGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAATTCGATGGCGGTGCCGATGAAGCTGGCGACTGCGACCC  >  NC_008463/1356401‑1356694
                                                                                                                                                   |                                                                                                                                                  
  cTGTCGTAGCCGGGGAGGACGCCGATCAGGGTGGTGGATACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAg                                                                                                                                                >  1:8485761/1‑150 (MQ=255)
     tCGTAGCCGGGGAGGACGCCGATCAGGGTGGTGGATACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTg                                                                                                                                             <  2:9809503/150‑1 (MQ=255)
     tCGTAGCCGGGGAGGACGCCGATCAGGGTGGTGGATACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTg                                                                                                                                             <  2:4588089/150‑1 (MQ=255)
       gTAGCCGGGGAGGACGCCGATCAGGGTGGTGGATACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAg                                                                                                                                           >  1:9220709/1‑150 (MQ=255)
        tAGCCGGGGAGGACGCCGATCAGGGTGGTGGATACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAgg                                                                                                                                          <  1:6691625/150‑1 (MQ=255)
          gCCGGGGAGGACGCCGATCAGGGTGGTGGATACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGaa                                                                                                                                        >  1:3382178/1‑150 (MQ=255)
            cGGGGAGGACGCCGATCAGGGTGGTGGATACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAgg                                                                                                                                      <  2:10684908/150‑1 (MQ=255)
                 aggaCGCCGATCAGGGTGGTGGATACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTg                                                                                                                                 <  2:8861305/150‑1 (MQ=255)
                   gaCGCCGATCAGGGTGGTGGATACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAg                                                                                                                               <  1:4408449/150‑1 (MQ=255)
                     cGCCGATCAGGGTGGTGGATACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCa                                                                                                                             <  1:8786970/150‑1 (MQ=255)
                     cGCCGATCAGGGTGGTGGATACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCa                                                                                                                             <  1:11106295/150‑1 (MQ=255)
                         gATCAGGGTGGTGGATACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTg                                                                                                                         >  1:10575314/1‑150 (MQ=255)
                         gATCAGGGTGGTGGATACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTg                                                                                                                         >  1:6949875/1‑150 (MQ=255)
                          aTCAGGGTGGTGGATACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTgc                                                                                                                        >  1:10062476/1‑150 (MQ=255)
                          aTCAGGGTGGTGGATACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTgc                                                                                                                        >  2:2506425/1‑150 (MQ=255)
                              gggTGGGGGATACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCgg                                                                                                                    <  2:7640934/150‑1 (MQ=255)
                               ggtggtGGATACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGt                                                                                                                   >  1:4135141/1‑150 (MQ=255)
                                 tggtggATACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGc                                                                                                                 <  1:551493/150‑1 (MQ=255)
                                  ggtggATACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGcc                                                                                                                >  2:10358450/1‑150 (MQ=255)
                                   gtggATACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCg                                                                                                               >  1:11051306/1‑150 (MQ=255)
                                     ggATACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGa                                                                                                             >  1:3558070/1‑150 (MQ=255)
                                     ggATACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGa                                                                                                             >  1:5285136/1‑150 (MQ=255)
                                      gATACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGAt                                                                                                            >  2:9420027/1‑150 (MQ=255)
                                      gATACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGAt                                                                                                            <  2:3746984/150‑1 (MQ=255)
                                        tACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGt                                                                                                          <  1:6614840/150‑1 (MQ=255)
                                        tACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGt                                                                                                          <  2:2072796/150‑1 (MQ=255)
                                             ccATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCg                                                                                                     >  1:9741284/1‑150 (MQ=255)
                                             ccATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCg                                                                                                     <  2:8372155/150‑1 (MQ=255)
                                             ccATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCg                                                                                                     <  2:7845682/150‑1 (MQ=255)
                                             ccATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCg                                                                                                     >  1:2211658/1‑150 (MQ=255)
                                              cctccccagccgcGAGGCGACCCGACGTGACGTGCGCCCGAGGCGGTCGCCGCAGTGGCCGACCAGCGCCGAGCCCACCGGGCGCGCGAGGAAGGCGATACCGAAGGTGAGGACGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCgg                                                                                                    <  2:5744052/144‑1 (MQ=255)
                                              cATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCgg                                                                                                    >  1:9924419/1‑150 (MQ=255)
                                                tCAGCAGCAGCGAGGCGACCAGGGGTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGga                                                                                                  <  1:6395445/150‑1 (MQ=255)
                                                  agcagcagcGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGgaag                                                                                                >  1:7616331/1‑150 (MQ=255)
                                                    cagcagcGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGgaagaa                                                                                              <  2:5931470/150‑1 (MQ=255)
                                                     agcagcGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGTCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGAGGTCTGCGGGAAGAAt                                                                                             <  2:7949285/150‑1 (MQ=255)
                                                     agcagcGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGACGAAt                                                                                             >  2:1061425/1‑150 (MQ=255)
                                                     agcagcGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAAt                                                                                             <  2:3250327/150‑1 (MQ=255)
                                                     agcagcGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAAt                                                                                             >  2:5419367/1‑150 (MQ=255)
                                                     agcagcGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAAt                                                                                             <  1:10931056/150‑1 (MQ=255)
                                                      gcagcGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATa                                                                                            >  2:6122361/1‑150 (MQ=255)
                                                        agcGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATAcc                                                                                          >  1:2894938/1‑150 (MQ=255)
                                                        agcGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATAcc                                                                                          >  2:5102592/1‑150 (MQ=255)
                                                            aGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGAc                                                                                      >  1:9991159/1‑150 (MQ=255)
                                                            aGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGAc                                                                                      <  1:10184358/150‑1 (MQ=255)
                                                             ggCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGAcc                                                                                     >  1:8002652/1‑150 (MQ=255)
                                                              gCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCg                                                                                    <  2:1149627/150‑1 (MQ=255)
                                                                 aCCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATc                                                                                 <  1:381450/150‑1 (MQ=255)
                                                                     gggTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCa                                                                             >  1:1774772/1‑150 (MQ=255)
                                                                     gggTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCa                                                                             <  2:4222631/150‑1 (MQ=255)
                                                                      ggTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAg                                                                            >  2:7040088/1‑150 (MQ=255)
                                                                           aCTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGggcgg                                                                       <  2:1648299/150‑1 (MQ=255)
                                                                                cgcCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCa                                                                  >  1:9314691/1‑150 (MQ=255)
                                                                                cgcCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCa                                                                  >  1:4957373/1‑150 (MQ=255)
                                                                                   ccGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTg                                                               <  2:965894/150‑1 (MQ=255)
                                                                                   ccGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTg                                                               <  1:1365952/150‑1 (MQ=255)
                                                                                    cGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTgg                                                              <  1:2261631/150‑1 (MQ=255)
                                                                                      aTGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCa                                                            <  2:1995566/150‑1 (MQ=255)
                                                                                       tGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGAGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCAt                                                           <  2:8141774/150‑1 (MQ=255)
                                                                                        gCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATa                                                          <  1:10372726/150‑1 (MQ=255)
                                                                                        gCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATa                                                          <  1:1948808/150‑1 (MQ=255)
                                                                                          ggTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGa                                                        <  2:5506418/150‑1 (MQ=255)
                                                                                           gTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCTGGGCGGCGGCAGTGGCATAGAc                                                       >  2:4946079/1‑150 (MQ=255)
                                                                                           gTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGAc                                                       >  1:5102019/1‑150 (MQ=255)
                                                                                             cGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGt                                                     >  2:7330879/1‑150 (MQ=255)
                                                                                              gCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTa                                                    <  2:4748427/150‑1 (MQ=255)
                                                                                               ccGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAg                                                   >  1:7805346/1‑150 (MQ=255)
                                                                                               ccGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAg                                                   >  1:2977266/1‑150 (MQ=255)
                                                                                                cGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGa                                                  <  1:1631193/150‑1 (MQ=255)
                                                                                                agAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATCCCGAAGGTGAGGAAGGCGCTGAGCATCGGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCAGAGACGTAGa                                                  >  2:2882127/2‑150 (MQ=255)
                                                                                                 gAAGTGGCCGAACCGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGaa                                                 <  2:3027802/150‑1 (MQ=255)
                                                                                                  aaGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGaaa                                                >  2:8797800/1‑150 (MQ=255)
                                                                                                  aaGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGaaa                                                >  2:6797403/1‑150 (MQ=255)
                                                                                                    gTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATc                                              >  1:9012022/1‑150 (MQ=255)
                                                                                                          gAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCAGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGaa                                        >  1:5981510/1‑150 (MQ=255)
                                                                                                           aaCAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAAt                                       <  1:1197308/150‑1 (MQ=255)
                                                                                                            aCAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAAtt                                      >  1:683194/1‑150 (MQ=255)
                                                                                                            aCAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAAtt                                      <  1:7252335/150‑1 (MQ=255)
                                                                                                             cAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGACGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCAGAGACGTAGAAATCGTAGAATTc                                     >  2:2213038/1‑150 (MQ=255)
                                                                                                                  ccGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAATTCGATgg                                >  2:853773/1‑150 (MQ=255)
                                                                                                                     aGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAATTCGATGGCgg                             >  1:4684864/1‑150 (MQ=255)
                                                                                                                     aGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAATTCGATGGCgg                             <  2:3493614/150‑1 (MQ=255)
                                                                                                                        ccAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCATGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAATTCGATGGCGGTGc                          >  1:10595974/1‑150 (MQ=255)
                                                                                                                         cAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAATTCGATGGCGGTGcc                         <  2:1774772/150‑1 (MQ=255)
                                                                                                                          aGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAATTCGATGGCGGTGCCg                        >  1:2794654/1‑150 (MQ=255)
                                                                                                                                 gagcgagGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAATTCGATGGCGGTGCCGATGAAGc                 <  2:9037025/150‑1 (MQ=255)
                                                                                                                                  agcgagGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAATTCGATGGCGGTGCCGATGAAGCt                >  2:8670189/1‑150 (MQ=255)
                                                                                                                                   gcgagGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAATTCGATGGCGGTGCCGATGAAGCTg               <  2:400450/150‑1 (MQ=255)
                                                                                                                                    cgagGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAATTCGATGGCGGTGCCGATGAAGCTgg              <  1:5818171/150‑1 (MQ=255)
                                                                                                                                    cgagGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAATTCGATGGCGGTGCCGATGAAGCTgg              <  2:6434269/150‑1 (MQ=255)
                                                                                                                                     gagGAAGGCGATACCGACGGTGAGGCAGGCGCTGAGCATCTGCGCGGGGCCGGATGTCTGCGGGAAGAAGACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAATTCGATGGCGGTGCCGATGAAGCTGGc             <  2:6636746/150‑1 (MQ=255)
                                                                                                                                       ggAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAATTCGATGGCGGTGCCGATGAAGCTGGCGa           >  1:705305/1‑150 (MQ=255)
                                                                                                                                       ggAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAATTCGATGGCGGTGCCGATGAAGCTGGCGa           <  2:11108800/150‑1 (MQ=255)
                                                                                                                                        gAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAATTCGATGGCGGTGCCGATGAAGCTGGCGAc          <  2:10143441/150‑1 (MQ=255)
                                                                                                                                         aaGGCGATACCGAAGGTGAGGAAGGCGCTGAGCTTCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAATTCGATGGCGGTGCCGATGAAGCTGGCGACt         <  2:6752871/150‑1 (MQ=255)
                                                                                                                                         aaGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAATTCGATGGCGGTGCCGATGAAGCTGGCGACt         <  2:4043627/150‑1 (MQ=255)
                                                                                                                                         aaGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAATTCGATGGCGGTGCCGATGAAGCTGGCGACt         <  1:3076431/150‑1 (MQ=255)
                                                                                                                                              gATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAATTCGATGGCGGTGCCGATGAAGCTGGCGACTGCGAc    >  1:8281591/1‑150 (MQ=255)
                                                                                                                                               aTACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGCCGTAGAAATCGTAGACTTCGATGGCGGTGCCGATGAAGCTGGCGACTGCGAcc   >  2:7553844/1‑150 (MQ=255)
                                                                                                                                                tACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAATTCGATGGCGGTGCCGATGAAGCTGGCGACTGCGAccc  <  2:6931110/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TGCTGTCGTAGCCGGGGAGGACGCCGATCAGGGTGGTGGATACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACGGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAATTCGATGGCGGTGCCGATGAAGCTGGCGACTGCGACCC  >  NC_008463/1356401‑1356694

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑